• 재활복지공학회논문지
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• 제7권1호
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• pp.21-27
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• 2013

수동 휠체어의 추진은 추진 동작의 낮은 효율로 인하여 사용자의 상지 관절에 고통과 부상까지 유발할 수 있다. 이에 따라 수동 휠체어 추진 중에 발생하는 상지 관절의 운동역학적 해석이 필요하다. 본 연구에서는 수동 휠체어 추진 중 상지 관절에 작용하는 토크를 구할 수 있는 2차원 역동역학 모델을 개발하였다. 개발한 모델은 시상면에서 상완, 하완, 손에 해당하는 3개의 체절로 상지를 구성하였고 몸통으로부터 3개의 체절을 회전조인트로 연결한 개방연쇄구조를 갖는다. 역동역학 해는 뉴턴-오일러 방법으로 구하였고 요구되는 입력자료는 실험을 통하여 획득하였다. 수동 휠체어 추진에 필요한 상지 거동의 운동학적 자료는 3차원 동작분석 시스템에서 추출하였고 역동역학 모델의 외력에 해당하는 운동역학적 자료는 브레이크식 다이나모미터에서 추출하였다. 역동역학 모델을 이용한 해석을 통하여 수동 휠체어 추진에 따른 상지 관절의 회전각과 관절 토크를 구하였다. 개발된 모델은 상지 관절에 관한 생체역학적 해석 도구이며 적은 노력으로 3차원 역동역학 모델로 확장하는 토대가 된다.

• 한국빅데이터학회지
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• 제3권1호
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• pp.73-82
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• 2018

미래 의학의 핵심은 개인을 중심으로 한 정밀의료(Precision Medicine)의 실현이다. 이것이 가능하기 위해서는 헬스케어 데이터를 언제 어디서나 열람, 관리 및 유통 할 수 있는 개방형 생태계를 갖추어야 한다. 하지만, 헬스케어 데이터는 민감한 개인정보를 다루기 때문에 상당한 수준의 신뢰성과 보안성이 동시에 요구된다. 이를 해결하기 위한 방안으로 최근 의료계에서도 블록체인 기술에 주목하고 있다. 블록체인 기술은 거래정보를 중앙 서버에 저장 및 관리하는 기존의 정보통신 인프라와는 달리 유통되는 데이터를 네트워크에 참여하는 모든 사용자가 분산 및 관리하는 방식의 분산 운영망 이다. 본 연구에서는 블록체인 기술을 이용해 헬스케어 데이터 유통 실증에 필요한 기술적 및 법리적 제반 사항, 코렌(KOREN SDI)망 기반 헬스케어 빅데이터 유통 실증 연구의 소개 및 헬스케어 분야에서 블록체인 기술을 활성화하기 위한 정책적 전략에 대해 논의한다.

• 컴퓨터교육학회논문지
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• 제18권2호
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• pp.21-33
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• 2015

본 논문의 목적은 오픈하드웨어 기반의 고고보드를 사용하여 IT융합 기반의 로봇교육용 콘텐츠를 설계 및 개발하고 프로젝트 수행 사례를 제공하는데 있다. 연구자는 3회에 걸쳐 프로젝트를 실시하였으며 초등학생 및 중학생들의 작품 사진, 산출물, 설문지 자료, 팀별 활동에 관한 비디오, 교수자 및 학생들의 면담 자료를 수집하여 분석하였다. 개별 학생은 고고보드를 활용하여 차체의 전면과 후면에 부착된 터치 센서의 입력신호로 모터를 구동하는 범퍼카를 직접 제작하는 과정을 통해 센싱에 의한 디지털 보드 제어 원리와 전동장치의 작동을 이해할 수 있었다. 참여 학생들은 후속 단계에서 도미노 게임과 3단계 주행구간에서 고고 면허증을 따는 팀별 활동을 수행하였다. 영재반 학생들은 자신의 아이디어를 새롭게 고안하여 로봇예술의 일환으로 개인 창작품을 제작하였다. 사례 분석 결과 제안된 프로젝트는 참여학생들 이 교수자의 면밀한 도움 아래 공작 체험이 디지털 보드 활용에 관한 기술에 대한 친밀감, 재미, 집중력을 제공하였고 더 나아가 자신의 아이디어를 점진적으로 발전시켜 창의적 작품을 만들어나가는 확장성을 보여주었다.

• 대한안전경영과학회지
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• 제5권3호
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• pp.81-98
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• 2003

In modern society, there are diverse sorts of VIPs including a king and individuals. Some like a king or president are in need of professional secret services, and others become VIPs by being guarded by a bodyguard company at their own request. The former is called a public guard, and the latter is a private one. In the field of the public guard, the nation should be furnished with a guard system in consideration of political circumstances to protect VIPs. That is, the parliamentary cabinet system and presidential system call for a different guard system, and the guard system's professional features vary to some degree with political situations and cultural climate. If the ultimate goal of guard is to protect the chief of the democratic and open state in a most efficient and safe manner, it's mandatorily necessary to set up a professional and specialized guard organization to provide democratic and efficient secret services. It's desired that the domestic presidential guard division should be staffed with not only expert guards but other personnels dispatched from the military and police. In the police, guard mission is splitted among various guard and security agencies, including the National Police Agency's public security bureau or the Seoul Metropolitan Police Agency's public security division, 101 security unit, mobile police and 22 special guard division. As a chain of command is separated and it's difficult to manage such organizations effectively, the National Police Agency should be exclusively in charge of VIP guard and security. In the area of private guard, the private police system has shared national security task as a middle organization between the private police and private guard. But now its intent has gradually weakened, and its mission should be redefined. In addition, the way people look at private guard should change, and they should be well informed that it could serve to prevent possible crimes, protect them against crimes and get rid of their inconveniences. To make a good impression on people, the guard associations and businesses should put their efforts into reinforcing publicity activities, and the government, academic circles and press should team up with them as well.

• Animal cells and systems
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• 제15권1호
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• pp.29-36
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• 2011

Insect lysozymes are basic, cationic proteins synthesized in fat body and hemocytes in response to bacterial infections and depolymerize the bacterial cell wall. The c-type lysozyme of the insect Spodoptera litura (SLLyz) is a single polypeptide chain of 121 residues with four disulfide bridges and 17 rare codons and is approximately 15 kDa. The full-length SLLyz cDNA is 1039 bp long with a poly(A) tail, and contains an open reading frame of 426 bp long (including the termination codon), flanked by a 54 bp long 5' UTR and a 559 bp long 3' UTR. As a host for the production of high-level recombinant proteins, E. coli is used most commonly because of its low cost and short generation time. However, the soluble expression of heterologous proteins in E. coli is not trivial, especially for disulfide-bonded proteins. In order to prevent inclusion body formation, GST was selected as a fusion partner to enhance the solubility of recombinant protein, and fused to the amplified products encoding mature SLLyz. The expression vector pGEX-4T-1/rSLLyz was then transformed into E. coli BL21(DE3)pLysS for soluble expression of rSLLyz, and the soluble fusion protein was purified successfully. Inhibition zone assay demonstrated that rSLLyz showed antibacterial activity against B. megaterium. These results demonstrate that the GST fusion expression system in E. coli described in this study is efficient and inexpensive in producing a disulfide-bonded rSLLyz in soluble, active form, and suggest that the insect lysozyme is an interesting system for future structural and functional studies.

• Journal of Microbiology
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• 제45권3호
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• pp.219-226
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• 2007

Insertion sequence IS1112 is a repetitive element with a relatively high number of copies in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice (Oryza sativa L.). Three new loci of IS1112 were identified in seven Chinese strains of Xoo using a single oligonucleotide primer J3; 5'-GCTCA GGTCAGGTGGCCTGG-3' by insertion-sequence-based polymerase chain reaction (IS-PCR). Among the three new loci of IS1112, two were located in the open-reading frame region of genes fhuA and cirA, which encode TonB-dependent receptors, and the third in ISXo2, another type of insertion sequence in Xoo genome. Three variants of IS1112 were identified in those three loci based on their sequence similarities: two were identical to IS1112a and IS1112b, reported in strain PXO86 from the Philippines, while the third was a new member of IS1112, defined as IS1112d. Inserting IS1112 in gene fhuA caused three bases, GGT, to be duplicated at the target site, but inserting it in gene cirA did not cause any duplication in the target site. The diversity of IS1112 sequence and insertion loci in Xoo genome and their potential effects are discussed.

• Environmental Analysis Health and Toxicology
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• 제26권
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• pp.5.1-5.11
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• 2011

Objectives: In order to identify the possibility of slender bitterling (SB) (Acheilognathus yamatsutae) being used as a test species for estrogenic endocrine disrupting chemicals (EEDCs), we carried out the cloning and sequence characterization of the estrogen receptor (ER). Methods: The ER from a slender bitterling was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR), 5'- and 3'-rapid amplification of cDNA ends (5'-RACE and 3'-RACE) and T-vector cloning. The expression of ER mRNA was also analyzed in six tissues (brain, liver, kidney, gill, gonad, and intestines) by real-time PCR. Results: We obtained an ER from the slender bitterling. The SB ER cDNA was 2189 base pairs (bp) in length and contained a 1707 bp open reading frame that encoded 568 amino acid residues. The SB ER amino acid sequence clustered in a monophyletic group with the $ER{\alpha}$ of other fish, and was more closely related to zebrafish $ER{\alpha}$(88% identity) than to the $ER{\alpha}$ of other fish. The SB ER cDNA was divided into A/B, C, D, E and F domains. The SB ER has conserved important sequences for ER functions, such as the DNA binding domain (D domain), which are consistent with those of other teleosts. Conclusions: The ER of the slender bitterling could provide basic information in toxicological studies of EEDCs in the slender bitterling.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2017년도 추계학술대회
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• pp.571-572
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• 2017

전자화폐를 구현하는 블록체인 기술은 분산화된 컴퓨팅을 사용하며 모든 거래가 모두에게 공개되어 있다. 이러한 기술은 사용자 대신 주소를 사용하여 거래에 수행하여 익명성을 보장하는 것처럼 보이지만 거래 그래프를 기반으로 비순환 방향 그래프를 이용하여 공개된 주소를 추적함으로써 프라이버시 문제가 발생하게 된다. 본 논문에서는 블록체인 기술 내에서 발생하는 프라이버스를 보호하기 위하여 그래프 상의 관련성을 찾기 어렵게 하는 중앙 집중적으로 처리하는 다양한 기법들을 분석한다. 또한, 프라이버시를 강화하기 위하여 분산화된 방법으로 익명화하는 기법들도 분석한다. 영지식 증명 기법을 이용하면 완전 분산 익명성을 보장하지만 더 많은 연산과 저장 공간이 필요하게 되며, 이를 효율적으로 하기 위한 여러 가지 기법들도 제안되었다. 본 논문에서는 기존 프라이버시 보호 기법들을 블록체인 기술에 융합하여 블록체인 기술의 프라이버시 보호를 위한 기법을 제안한다.

• The Plant Pathology Journal
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• 제36권6호
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• pp.618-627
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• 2020

Although limited progress have been made about pathogen system of Hibiscus rosa-sinensis and Hibiscus latent Fort Pierce virus (HLFPV), interaction between plant host and pathogen remain largely unknown, which led to deficiency of effective measures to control disease of hibiscus plants caused by HLFPV. In this study, infection of HLFPV in Hibiscus rosa-sinensis was firstly confirmed for the first time by traditional electron microscopy, modern reverse transcription polymerase chain reaction and RNA-seq methods in China (HLFPV-Ch). Sequence properties analyzing suggested that the full-length sequences (6,465 nt) of HLFPV-Ch had a high sequence identity and a similar genomic structure with other tobamoviruses. It includes a 5'-terminal untranslated region (UTR), followed by four open reading frames encoding for a 128.5-kDa replicase, a 186.5-kDa polymerase, a 31-kDa movement protein, 17.6-kDa coat protein, and the last a 3'-terminal UTR. Furthermore, HLFPV-Ch-derived virus-derived siRNAs (vsiRNAs) ant its putative target genes, reported also for the first time, were identified and characterized from disease Hibiscus rosa-sinensis through sRNA-seq and Patmatch server to investigate the interaction in this pathogen systems. HLFPV-Ch-derived vsiRNAs demonstrated several general and specific characteristics. Gene Ontology classification revealed predicted target genes by vsiRNAs are involved in abroad range of cellular component, molecular function and biological processes. Taken together, for first time, our results certified the HLFPV infection in China and provide an insight into interaction between HLFPV and Hibiscus rosa-sinensis.

• Journal of Microbiology
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• 제43권5호
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• pp.406-410
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• 2005

In contrast to Saccharomyces cerevisiae, little is known about the kinesin-like protein (KLP) in Candida albicans. The motor domain of kinesin, or KLP, contains a subregion, which is well conserved from yeast to humans. A similarity search, with the murine ubiquitous kinesin heavy chain region as a query, revealed 6 contigs that contain putative KLPs in the genome of C. albicans. Of these, the length of an open reading (ORF) of 375 amino acids, temporarily designated CaKAR3, was noticeably short compared with the closely related S. cerevisiae KAR3 (ScKAR3) of 729 amino acids. This finding prompted us to isolate a ${\lambda}$ genomic clone containing the complete CaKAR3 ORF, and here the complete sequence of CaKAR3 is reported. CaKAR3 is a C-terminus motor protein, of 687 amino acids, encoded by a non-disrupting gene. When compared with ScKAR3, the amino terminal region of 112 amino acids was unique, with the middle part of the 306 amino acids exhibiting $25\%$ identity and $44\%$ similarity, while the remaining C-terminal motor domain exhibited $64\%$ identity and $78\%$ similarity, and have been submitted to GeneBank under the accession number AY182242.