• Title/Summary/Keyword: open culture system

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Cloning, Sequencing and Baculovirus-based Expression of Fusion-Glycoprotein D Gene of Herpes Simplex Virus Type 1 (F)

  • Uh, Hong-Sun;Choi, Jin-Hee;Byun, Si-Myung;Kim, Soo-Young;Lee, Hyung-Hoan
    • BMB Reports
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    • v.34 no.4
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    • pp.371-378
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    • 2001
  • The Glycoprotein D (gD) gene of the HSV-1 strain F was cloned, sequenced, recombinated into the HcNPV (Hyphantria cunea nuclear polyhedrosis virus) expression vector and expressed in insect cells. The gD gene was located in the 6.43 kb BamHI fragment of the strainF. The open reading frame (ORF) of the gD gene was 1,185 by and codes 394 amino acid residues. Recombinant baculoviruses, GD-HcNPVs, expressing the gD protein were constructed. Spodoptera frugiperda cells, infected with the recombinant virus, synthesized a matured gX-gD fusion protein with an approximate molecular weight of 54 kDa and secreted the gD proteins into the culture media by an immunoprecipitation assay The fusion gD protein was localized on the membrane of the insect cells, seen by using an immunofluorescence assay The deduced amino acid sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane sequence. These results indicate the utility of the HcNPV-insect cell system for producing and characterizing eukaryotic proteins.

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Effect of in vitro B-6 Vitameric Forms on Lymphocyte Proliferation in Healthy Young Women with Oral Vitamin B-6 Supplementation

  • Kwak Ho Kyung;Leklem James E.
    • Journal of Community Nutrition
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    • v.7 no.2
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    • pp.79-84
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    • 2005
  • A vitamin B-6 (B-6) intake higher than the current Recommended Dietary Allowance (RDA) has been found to provide an improvement in immune system. Seven premenopausal women consumed their usual diet with the exception of foods relatively high in vitamin B-6 for a total of 27 d. After 7 d, all subjects received a multivitamin supplement containing 2mg B-6 and 4 subjects were given an additional 50mg of B-6 supplement for 20 d. Lymphocyte response to phytohemagglutinin (PHA) was measured before and after the supplementation. To determine the effect of different forms of B-6 on lymphocyte proliferation, cell culture media supplemented with pyridoxal (PL) and PLP, as well as B-6 free media, were tested. A 50mg B-6 supplement significantly increased vitamin B-6 status. There was no further enhancement on lymphocyte proliferation when subjects were taking an additional 50mg of vitamin B-6 supplement. In general, lymphocyte proliferation in media with either PLP or PL did not show any prominent difference. These [m-dings suggest that there may be no further benefits of a B-6 dose beyond twice that of the current RDA on lymphocyte proliferation. Further studies are necessary to examine the effect of the B-6 intake level on activities of enzymes involved in cellular B-6 metabolism in lymphocytes to provide substantial insight into the mechanisms underlying the role of B-6 in the lymphocyte proliferation.

A Study on the Characteristics of Interior Materials for the Public Area in the City Hotels - Focused on First Class Hotels in Seoul - (도시호텔 공용공간 실내건축의 내장재 특성에 관한 연구 - 서울시 특1급 호텔을 중심으로 -)

  • Jeun, Uoo-Kyoung;Lee, Chang-No
    • Proceedings of the Korean Institute of Interior Design Conference
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    • 2007.11a
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    • pp.149-154
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    • 2007
  • The city hotel of today lodges and, from eating and drinking putting first cultural exchange, information transmission and sports and the leisure back to be various the open community of the city which equips a composition facility as the market fixation it is being turning out. Like this regional and culture flow scope of the user fall in love, width widely try to be diffused the data about the internal organs re-analysis our country hotel plan the place where it is the actual condition where the system and diverse royal tomb city hotel plan is coming to be demanded regarding very is the actual condition which is insufficient. The research which it sees hereupon from domestic is erected in existing and the city hotel which is coming to be operated re- internal organs in the object it investigate, analyze re- internal organs and new city hotel plan hour to present a plan fundamental data and the guide regarding the internal organs re-quality of the crew-serve space interior construction it could be applied there is an objective of the research by the box sleep.

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Visual Information Tactile Transformation Display to Expand the Enjoyment of Art and Culture for the Blind (시각장애인 예술 문화 향유 확장을 위한 시각 정보 촉각 변환 디스플레이)

  • Sang-Don Lee;Ju-Hyeon Lee;Jae-Hyeong Hwang;Hyeon-Jung Hwang;Jae-Hun Lee
    • Annual Conference of KIPS
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    • 2023.11a
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    • pp.996-997
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    • 2023
  • 시각 장애인들의 시각 정보에 대한 낮은 접근성은 문화, 예술 활동에 큰 제약을 가져다 주고 있다. 실제로 시각 장애인 중 약 절반 이상이 문화, 여가생활에 만족하지 못한다고 답하였고 전시회, 미술품 감상 또는 관람 활동은 약 5%만이[1] 참여하고 있는 것으로 나타났다. 이러한 한계를 극복하기 위해 시각이라는 감각의 한계를 뛰어넘어 시각 미디어를 즐길 수 있게 하는 서비스를 제작하였고, 이는 크게 웹서비스인 web view editor와 물리적인 촉각 디스플레이로 구성된다. 시각 미디어인 이미지는 8×8로 나눠 각 영역을 OpenCV 라이브러리와 K-means clustering 알고리즘을 이용하여 9 level로 분류시키고, 구분된 level에 맞게 cell의 높낮이 차이를 두기 위하여 Arduino를 통한 회전-선형 변환기를 제작했다. Arduino의 PWM 기능을 이용해 모터의 속도와 방향을 제어하며, 각 모터의 드라이버는 Arduino와 연결되어 있어 모터의 회전을 제어하게 했다. 결과적으로 본 연구에서는 cell의 높낮이 차이를 9 level로 구분하여 시각 정보를 촉각으로 수용할 수 있는 장치를 제작하였고, 이 장치를 통해 기존의 시각 장애인들이 문화 생활을 쉽게 향유하고 이를 바탕으로 창의성과 상상력을 증대시켜 더욱 밀접하게 사회와 연결되고 소통 할 수 있는 기회의 초석이 되기를 기대하는 바이다.

A Case Study of Digital Transformation: Focusing on the Financial Sector in South Korea and Overseas

  • Eunchan Kim;Minjae Kim;Yeunwoong Kyung
    • Asia pacific journal of information systems
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    • v.32 no.3
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    • pp.537-563
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    • 2022
  • This study investigates the adoption and application of digital transformation in the financial sector and analyzes the process and outcomes of digitization and digitalization in the field of the finance industry of South Korea and overseas, in order to seek both managerial and strategic implications for successful implementation of digital transformation in the future. The findings show that, for successful digital transformation, it is necessary to maximize active and systematic use of advanced online and digital technologies that form the basis of business and create an open, horizontal organizational culture and communication system to equally share and distribute advanced technologies and competencies through the entire organization. Furthermore, this study also discovers the legitimacy to concentrate the organizational competencies and know-how in providing technical training for members, expanding customer experience, and improving customer satisfaction services to contribute to improving the quality of life for members of the organization and creating and improving social and public infrastructures, instead of using digital transformation only to improve productivity of organizations or firms. As such, it is necessary to concentrate corporate competencies in establishing and supplying digital transformation that is not just human-centered but also has productivity, innovativeness, and reliability at the same time.

A study on the development of surveillance system for multiple drones in school drone education sites (학내 드론 교육현장의 다중드론 감시시스템 개발에 관한 연구)

  • Jin-Taek Lim;Sung-goo Yoo
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.1
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    • pp.697-702
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    • 2023
  • Recently, with the introduction of drones, a core technology of the 4th industrial revolution, various convergence education using drones is being conducted in school education sites. In particular, drone theory and practice education is being conducted in connection with free semester classes and career exploration. The drone convergence education program has higher learner satisfaction than simple demonstration and practice education, and the learning effect is high due to direct practical experience. However, since practical education is being conducted for a large number of learners, it is impossible to restrict and control the flight of a large number of drones in a limited place. In this paper, we propose a monitoring system that allows the instructor to monitor multiple drones in real time and learners to recognize collisions between drones in advance when multiple drones are operated, focusing on education operated in schools. The communication module used in the experiment was equipped with GPS in Murata LoRa, and the server and client were configured to enable monitoring based on the location data received in real time. The performance of the proposed system was evaluated in an open space, and it was confirmed that the communication signal was good up to a distance of about 120m. In other words, it was confirmed that 25 educational drones can be controlled within a range of 240m and the instructor can monitor them.

A Study on the Spatial Composition and Expressive Characteristics in Mass-Complex Commercial Space by Jerde Partnership's - Through Analyzing the Space Structure by Space Syntax - (저드 파트너쉽의 대규모 복합상업공간에서 나타나는 공간구성과 표현특성에 관한 연구 - 공간구문론에 의한 공간구조 분석을 통해서 -)

  • Kim, Yun-Jung;Jang, So-Eun;Park, Chan-Il
    • Korean Institute of Interior Design Journal
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    • v.19 no.6
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    • pp.188-196
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    • 2010
  • The Commercial space are changed gradually large scalized and complexed to introduce different culture exchanges and experience. However, the mass-complex commercial space are limited as of their complexed space composition or degree of space depth, which may become factors causing people to get tired easily. Thus, having a design strategy is important to create economical effectiveness in order to overcome these limitations and for the people to stay longer and do their consumption activities through more experiences and social interchanges. Recently, Jerde Partnership's who designed many complex commercial spaces made a commercial success by their unique design strategy to setup a new space and lead the interaction between space and people. The purpose of this study is based on Jerd's design concept to analyze Jerd Partnership's design strategy and space related structure to propose their space construction and design method for mass-complex commercial spaces. The results are as follows. (1)Jerde Partnership's causes the abundant space experience of the user through mass and space constitution to have a theme and a story. (2)They builds an excursion type line of flow system with an organic curve, and a non-daily experience by the change of the space scale and the application of various programs is enabled and guides the stay for the long time. (3)They builds the doorway of various courses and a circulation system through the open space and controls depth of the space. In addition, various events are performed in the center of such a circulation system, and this event makes a unique place.

A study on the developing and implementation of the Cyber University (가상대학 구현에 관한 연구)

  • Choi, Sung;Yoo, Gab-Sang
    • Proceedings of the Technology Innovation Conference
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    • 1998.06a
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    • pp.116-127
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    • 1998
  • The Necessity of Cyber University. Within the rapidly changing environment of global economics, the environment of higher education in the universities, also, has been, encountering various changes. Popularization on higher education related to 1lifetime education system, putting emphasis on the productivity of education services and the acquisition of competitiveness through the market of open education, the breakdown of the ivory tower and the Multiversitization of universities, importance of obtaining information in the universities, and cooperation between domestic and oversea universities, industry and educational system must be acquired. Therefore, in order to adequately cope wi th these kinds of rapid changes in the education environment, operating Cyber University by utilizing various information technologies and its fixations such as Internet, E-mail, CD-ROMs, Interact ive Video Networks (Video Conferencing, Video on Demand), TV, Cable etc., which has no time or location limitation, is needed. Using informal ion and telecommunication technologies, especially the Internet is expected to Or ing about many changes in the social, economics and educational area. Among the many changes scholars have predicted, the development and fixations of Distant Learning or Cyber University was the most dominant factor. In the case of U. S. A., Cyber University has already been established and in under operation by the Federate Governments of 13 states. Any other universities (around 500 universities has been opened until1 now), with the help of the government and private citizens have been able to partly operate the Cyber University and is planning on enlarging step-by-step in the future. It could be seen not only as U. S. A. trying to elevate its higher education through their leading information technologies, but also could be seen as their objective in putting efforts on subordinating the culture of the education worldwide. UTRA University in U. S. A., for example, is already exporting its class lectures to China, and Indonesia regions. Influenced by the Cyber University current in the U.S., the Universities in Korea is willing .to arrange various forms of Cyber Universities. In line with this, at JUNAM National University, internet based Cyber University, which has set about its work on July of 1997, is in the state of operating about 100 Cyber Universities. Also, in the case of Hanam University, the Distant Learning classes are at its final stage of being established; this is a link in the rapid speed project of setting an example by the Korean Government. In addition, the department of education has selected 5 universities, including Seoul Cyber Design University for experimentation and is in the stage of strategic operation. Over 100 universities in Korea are speeding up its preparation for operating Cyber University. This form of Distant Learning goes beyond the walls of universities and is in the trend of being diffused in business areas or in various training programs of financial organizations and more. Here, in the hope that this material would some what be of help to other Universities which are preparing for Cyber University, I would 1ike to introduce some general concepts of the components forming Cyber University and Open Education System which has been established by JUNAM University. System of Cyber University could be seen as a general solution offered by tile computer technologies for the management on the students, Lectures On Demand, real hour based and satellite classes, media product ion lab for the production of the multimedia Contents, electronic library, the Groupware enabling exchange of information between students and professors. Arranging general concepts of components in the aspect of Cyber University and Open Education, it would be expressed in the form of the establishment of Cyber University and the service of Open Education as can be seen in the diagram below.

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A Study of the City of Guangzhou in the Geographical Perspecives (광저우(廣州)에 대한 지리적 고찰(考察))

  • Sohn, Yong-Taek
    • Journal of the Economic Geographical Society of Korea
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    • v.14 no.3
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    • pp.407-418
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    • 2011
  • The southern region including Guangzhou(黃州), the capital of Guangdong Province, was called "South of the Mountain Range" (嶺南) because it is located south of the Five Mountain Ranges (五嶺) which made mobility difficult prior to unification of China by Qin dynasty (秦) in 221 BCE. The Guangdong region of the South of the Mountain Range (嶺南) was an administratively independent unit and, as such, called the Nanyue kingdom (南越國). This is the origin of the Chinese character "yue", and terms such as yueyu (Cantonese), yuecai (Cantonese cuisine), yueju (Cantonese opera) are still in use today. Guangzhou, called Panyu (番禹) during the Nanyue kingdom period, was settled early in the northern part of the Pearl River (珠江, Zhu Jiang) delta. It became the first port to open its doors to the great powers of the West during the Qing period. Although it has now fallen behind Shanghai which developed later, Guangzhou is still the third largest city after Beijing and Shanghai, and thus, it is an influential open coastal city. Today, not only is Guangzhou the world center of the light textile industry, it also spurs development in various manufacturing industries. Along with nearby Hong Kong, Macao, and Shenzhen, it plays the role of cradle of the Pearl River delta economic zone. Firmly established early on as the greatest central city in southern China, Guangzhou is simultaneously a hub in various aspects such as regional politics, economics, and culture; it was also the center of revolution and resistance which attempted to challenge the northern political powers. Guangzhou is known for the history of the rise and fall of its port, but with developmental efforts, it still maintains its influence. Guangzhou's dynamic development of today brings with it issues such as the environment and moral system which must be dealt with.

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Design and Implementation of IEC62541-based Industry-Internet of Things Simulator for Meta-Factory (메타팩토리를 위한 IEC62541기반 IIoT·시뮬레이터 설계 및 구현)

  • Chae-Young Lim;Chae-Eun Yeo;Woo-jin Cho;Jae-Hoi Gu;Sang-Hyun Lee
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.3
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    • pp.789-795
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    • 2023
  • Digital-Twin are recognized as an important core technology for the realization of Smart Factories by simulating and optimizing the monitoring and predictive maintenance of manufacturing equipment and the operation of production lines in a digital space. To implement this system, we adopt the IEC62541-based OPC-UA (Open Platform Communications Unified-Architecture) Protocol, which has strengths in interoperability and connectivity between heterogeneous platforms. Therefore, In this paper, We designed and implemented an IIoT(Industry Internet of Things) system that connects heterogeneous platforms, and developed an OPC-UA simulator based on IEC 62541. We will present whether the data will be applied to the Digital-Twin Platform and whether it will work, and proceed with performance tests and evaluations. We evaluate the operation performance and OPC-UA performance of the Digital-Twin platform lightened by the proposed device, and present the optimal IEC62514-based simulator system. We proceeded with the performance evaluation of sending and receiving data with OPC-UA wrapping with the proposed simulator, and found that a lightweight Digital-Twin platform can be operated. This research can apply the OPC-UA protocol for implementing smart factory and meta-factory in the manufacturing shop floor with limited resources, avoiding the waste of time and space on the shop floor through the OPC-UA simulator. We expect that this will contribute to a significant improvement in efficiency by minimizing.