• Title/Summary/Keyword: onion seed

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Development of a PCR Diagnostic System for Iris yellow spot tospovirus in Quarantine

  • Shin, Yong-Gil;Rho, Jae-Young
    • The Plant Pathology Journal
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    • v.30 no.4
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    • pp.440-444
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    • 2014
  • Iris yellow spot virus (IYSV) is a plant pathogenic virus which has been reported to continuously occur in onion bulbs, allium field crops, seed crops, lisianthus, and irises. In South Korea, IYSV is a "controlled" virus that has not been reported, and inspection is performed when crops of the genus Iris are imported into South Korea. In this study, reverse-transcription polymerase chain reaction (RT-PCR) and nested PCR inspection methods, which can detect IYSV, from imported crops of the genus Iris at quarantine sites, were developed. In addition, a modified positive plasmid, which can be used as a positive control during inspection, was developed. This modified plasmid can facilitate a more accurate inspection by enabling the examination of a laboratory contamination in an inspection system. The inspection methods that were developed in this study are expected to contribute, through the prompt and accurate inspection of IYSV at quarantine sites to the plant quarantine in South Korea.

Serratia plymuthica Strain A2l-4: A Potential Biocontrol Agent Against Phytophthora Blight of Pepper

  • Shen, Shun-Shan;Kim, Jin-Woo;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.138-141
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    • 2002
  • A promising biocontrol agent, A2l-4, against Phytophthora blight of pepper was selected from 351 bacterial isolates collected from rhizosphere soils and roots of onion (Allium fistulosum L.). The isolate A21-4 was identified as Serratia plymuthica based on its 16S rRNA sequence and key characteristics as compared with that of an authentic culture of S. plymuthica (ATCC No. 6109D01). The isolate readily colonized on roots of various crops including pepper when inoculated on seed and not. Strain A2l-4 showed narrow spectrum of antibiotic activity, as revealed in its strong inhibitory activity to the genera Pythium and Phytophthora, but not to Fuasrium and Rhizoctonia. In pot experiments, none of the pepper seedlings treated with A2l-4 were infected by Phytophthora capsici, while 86% of the control plants were killed by the pathogen.

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri

  • Nanda, Satyabrata;Chand, Subodh Kumar;Mandal, Purander;Tripathy, Pradyumna;Joshi, Raj Kumar
    • The Plant Pathology Journal
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    • v.32 no.6
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    • pp.519-527
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    • 2016
  • Purple blotch, caused by Alternaria porri (Ellis) Cifferi, is a serious disease incurring heavy yield losses in the bulb and seed crop of onion and garlic worldwide. There is an immediate need for identification of effective resistance sources for use in host resistance breeding. A total of 43 Allium genotypes were screened for purple blotch resistance under field conditions. Allium cepa accession 'CBT-Ac77' and cultivar 'Arka Kalyan' were observed to be highly resistant. In vitro inoculation of a selected set of genotypes with A. porri, revealed that 7 days after inoculation was suitable to observe the disease severity. In vitro screening of 43 genotypes for resistance to A. porri revealed two resistant lines. An additional 14 genotypes showed consistent moderate resistance in the field as well as in vitro evaluations. Among the related Allium species, A. schoenoprasum and A. roylei showed the least disease index and can be used for interspecific hybridization with cultivated onion. Differential reaction analysis of three A. porri isolates (Apo-Chiplima, Apn-Nasik, Apg-Guntur) in 43 genotypes revealed significant variation among the evaluated Allium species (P = 0.001). All together, the present study suggest that, the newly identified resistance sources can be used as potential donors for ongoing purple blotch resistance breeding program in India.

Use of Functional Films for Storage of Seed Tuber in Liriope platyphylla (맥문동 뿌리줄기의 저장력 향상을 위한 기능성필름 활용)

  • Seong, Eun Soo;Choi, Jae Hoo;Kim, Hee Kyu;Choi, Seung Hyuk;Kim, Chul Joong;Lee, Jae Geun;Yoo, Ji Hye;Kim, Na Young;Yu, Chang Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.4
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    • pp.296-301
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    • 2018
  • Background: The objective of this study was to investigate the effect of packaging material on the growth of rootstock of Liriope platyphylla. Methods and Results: This study examined the effects of two types of packaging material, LDPE (low density polyethylene) and functional film on the growth of the tubers of L. platyphylla, at $5^{\circ}C$. During the 16-weeks of storage period, the ratio of loss and decay of the tubers was examined at intervals of 4, 8, and 16 weeks to detect the quality of the plant. After 16 weeks of storage, the treated tubers were own. Subsequently, plant height and the number of leaves were recorded. The results revealed that functional film at $5^{\circ}C$ was the ideal material for the storage of L. platyphylla tubers. The rate of loss was the highest (57.42%) with a onion net and the lowest (22.12%) with a functional film. Similarly, the rate of tuber decay was highest (8.20%) using onion net and the least (4.60%) when the functional film was used. Conclusions: Thus, the use of the functional film proved to be the most effective in the storage of L. platyphylla tubers when compared with the LDPE.

Changes in Preference for Grape Seed Oil Dressing according to Mirepoix au Maigre Content Level (Mirepoix Au Maigre 함량 수준에 따른 포도씨유 드레싱의 수용도 변화)

  • Yoo, Seung-Seok;Seo, Min-Suk
    • Korean journal of food and cookery science
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    • v.23 no.5
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    • pp.685-695
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    • 2007
  • The purpose of this study was to develop an excellent salad dressing using grape seed oil, which contains high levels of unsaturated fatty acids. First, a vinaigrette was made with grape seed oil and vinegar and divided into five portions. Then, five types of mirepoix au maigre ($M_1,M_2,M_3,M_4,M_5$) were made with apple, onion, carrot, garlic, tomato puree, and tomato ketchup by mixing in a main material vessel. The samples were then allowed to ripen for three days. The control group $M_1$ was named $GD_1$, and the experimental groups $M_2,\;M_3,\;M_4,\;and\;M_5$ were named $GD_2,\;GD_3,\;GD_4,\;and\;GD_5$. respectively. To measure receptiveness, a sensory test was conducted using a 7 point category scale. The test concentrated on flavor, taste, color, agreeability, aftertaste, and overall acceptability: the panel consisted of 15 subjects According to the results, $GD_4$ had the best flavor, color and agreeability. The samples with the most preferred taste and aftertaste were $GD_4\;and\;GD_5$. Between $GD_4\;and\;GD_5$, there were no noticeable differences in taste, aftertaste, or overall acceptability, but differences in color and agreeability did exist. Thus, $GD_4$ proved to be the most favorable product. To determine the best complementing vegetables for $GD_4$ yam($VE_1$), broccoli sprouts($VE_2$), mustard leaves($VE_3$), beet leaves($VE_4$), cucumber($VE_5$), and lettuce(VE6) were selected as samples, and a sensory test was conducted. Each vegetable has its own peculiarities. According to the results, $VE_2$ had the nicest vegetable fragrance recording the highest mean value. The vegetable with the most preferred taste, aftertaste, and agreeability were $VE_2\;and\;VE_6$. $VE_1\;and\;VE_6$ had the nicest color and contained the brightest color as well. The highest overall acceptability was awarded to $VE_2\;and\;VE_6$ and there was no remarkable difference between thorn at a level of 0.05. In conclusion, $VE_2\;and\;VE_6$ were the best complementing vegetables with grape seed oil dressing. However, to commercialize such a dressing, many follow-up studies must be conducted.

Analysis of Literatures Related to Crop Growth and Yield of Onion and Garlic Using Text-mining Approaches for Develop Productivity Prediction Models (양파·마늘 생산성 예측 모델 개발을 위한 텍스트마이닝 기법 활용 생육 및 수량 관련 문헌 분석)

  • Kim, Jin-Hee;Kim, Dae-Jun;Seo, Bo-Hun;Kim, Kwang Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.23 no.4
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    • pp.374-390
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    • 2021
  • Growth and yield of field vegetable crops would be affected by climate conditions, which cause a relatively large fluctuation in crop production and consumer price over years. The yield prediction system for these crops would support decision-making on policies to manage supply and demands. The objectives of this study were to compile literatures related to onion and garlic and to perform data-mining analysis, which would shed lights on the development of crop models for these major field vegetable crops in Korea. The literatures on crop growth and yield were collected from the databases operated by Research Information Sharing Service, National Science & Technology Information Service and SCOPUS. The keywords were chosen to retrieve research outcomes related to crop growth and yield of onion and garlic. These literatures were analyzed using text mining approaches including word cloud and semantic networks. It was found that the number of publications was considerably less for the field vegetable crops compared with rice. Still, specific patterns between previous research outcomes were identified using the text mining methods. For example, climate change and remote sensing were major topics of interest for growth and yield of onion and garlic. The impact of temperature and irrigation on crop growth was also assessed in the previous studies. It was also found that yield of onion and garlic would be affected by both environment and crop management conditions including sowing time, variety, seed treatment method, irrigation interval, fertilization amount and fertilizer composition. For meteorological conditions, temperature, precipitation, solar radiation and humidity were found to be the major factors in the literatures. These indicate that crop models need to take into account both environmental and crop management practices for reliable prediction of crop yield.

A Research on Porridge that King Yeongjo Had Been Served - Based on The Daily Record of Royal Secretariat of Joseon Dynasty during King Yeongjo period - (영조가 복용한 죽(粥)에 대한 고찰 - 『승정원일기(承政院日記)』의 영조 기록을 중심으로 -)

  • Eom, Dongmyung;Kim, Yeonghyeon;Song, Jichung
    • Journal of Korean Medical classics
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    • v.30 no.1
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    • pp.17-29
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    • 2017
  • Objectives : Many curative methods are used utilized in order to rid human body of disease when people become sick. Traditional Korean medicine generally prescribe methods that involve acupunture, moxibustion, or herb formulae. However, different types of foods are sometimes used as well. While wondering the history and efficacy of Qi-elevation foods that were consumed by Koreans in the past, the author discovered that a record from the Joseon Dynasty, called The Daily Record of Royal Secretariat of Joseon Dynasty, henceforth the Records, contained information about different types of porridge. Hence, the author looked through the records of porridge as written in the historical material in an attempt to learn the examples and efficacy of medicine-porridge consumed in Joseon's royal palace. Methods : After searching for the keyword, 'porridge', in the Records as provided by the National Institute of Korean History, the author extracted the porridges recorded during the Yeongjo period that each has its own special name. Results : Different types of porridge were recorded in the Records as following: arrowroot porridge, bean-leaf porridge, mung bean porridge, bean porridge, malt-rice porridge, oriental arborvitae seed porridge, crucian porridge, lotus seed porridge, adlay porridge, red bean porridge, welsh onion porridge, milk porridge, seashell porridge, ginko nut porridge, black sesame porridge, and mandarin porridge. Each porridge was used for the purpose of alleviating any disease that afflicted the king Yeongjo or his royal family members in relation with the ingredient herb's medicinal function. Conclusions : These porridges consumed by the king Yeongjo and his royal family members were used not only with a purpose of aiding their body's recovery from disease, but with the goal to actively curing them of ailments.

Identification of Morphological Appearance of Minute Seed Herbs Using Stereoscope (Report II) - Alli Tuberosi Semen✳Alli Fistulosi Semen, Malvae Semen✳Abutili Semen, Plantaginis Semen✳Schizonepetae Semen (Stereoscope를 이용한 미세종자류 한약재 외부형태 감별연구(제2보(報)) -구자(韭子)와 총자(蔥子), 동규자(冬葵子)와 경마자(苘麻子), 차전자(車前子)와 형개자(荊芥子)-)

  • Kim, Young-Sik;Ju, Young-Sung
    • The Korea Journal of Herbology
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    • v.31 no.4
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    • pp.61-69
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    • 2016
  • Objectives : This study is to propose discriminative keys with stereoscope for minute seed herbs easily mixed up, but hard to discriminate by naked eyes: Alli Tuberosi Semen(ATS)✳Alli Fistulosi Semen(AFS), Malvae Semen(MS)✳ Abutili Semen(AS), Plantaginis Semen(PS)✳Schizonepetae Semen(SS).Objectives : We reviewed the description of original plants and medicinal herbs from literature. The specimen of original plant were collected, determinated and compared to samples in the market. Primary classification was performed with naked eyes. and we found out other discrimination keys for non-distinctive herbs with stereoscope. Discrimination keys were set as the morphological criteria of authentic herbs, percentage of adulteration, and standards for discriminating genuine herbs from adulteration.Results : 1) ATS is bigger, has reticulate pattern on protuberant side and unique garlic chives taste. AFS which is usually mixed up, is smaller, has 1~2 ridges and unique welsh onion taste. 2) MS is smaller, has no villus, but its length of bumps are similar. AS easily mixed up, is bigger and has white villus. Its upper side bump is longer and more sharp. 3) PS has dent at middle of the back side. It becomes very sticky when it is put or boiled in water. SS which can be mixed up has no dent and low viscosity compared to PS.Conclusions : With this result, we propose discriminative keys which can identify the original plants and processed herbal state of six herbs. Because minute medicinal herbs are hardly distinguishable by sensory test, It is essential to differentiate by using stereoscope.

The Growth Characteristics and Yield of Treatment Onion (Allium cepa L.)Seed in Direct Sowing Cultivation (종자처리 양파(Allium cepa L.)의 포장직파재래 생육 특성)

  • 이성춘
    • Korean Journal of Plant Resources
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    • v.14 no.2
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    • pp.94-101
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    • 2001
  • This study was intended to evaluate the relationship environmental condition and growth characteristics of seed treatment seed in direct sowing with different sowing date, and to suggest new cultivation model. The emergence percentage and hours were down as the delayed sowing date. Optimum sowing time was Sep. 10, and sowing must end before Sep. 20 for safety yield. When did sowing ended before Sep.20 in direct sowing cultivation, the seedling growth(SG) and No of roots of direct sowing seedling were larger than transplanting cultivation . At March 8 evaluated seedling, the SG of direct sowing seedling was higher than transplanting, and at Sep. 2(1 those were similar. The missing plant rate in transplanting cultivation was under 3 %, and that in direct sowing cultivation was about 13∼18%, and the extent were sever as delayed sowing date. The bulb fresh weight was decreased as delayed sowing, and those in direct sowing and transplanting cultivation were 230, 217g, respectively. The blot rate was high in direct sowing cultivation, and that was decreased as delayed sowing. The yield in direct sowing and transplanting cultivation were 5,134, 5,300kg, respectively, and those were decreased as delayed sowing. The average yield in early and medium-late maturity cultivars were 3,750, 4,908kg, respectively, and Sonic was highest yield in early maturity cultivars and Nongwoodego was highest in medium-late maturity cultivars. The emergence weed were 9 species as Loportea bulbifera Weddell and others, and Loported bulbifera Weddell, Digitaria violascens Link and Persicaria longiseta Kitagawa were dominant species in direct sowing cultivation with black hole vinyl mulching

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An Etiological Study on the Anthracnose Fungus of Pepper Caused by Colletotrichum dematium in Korea (고추에서 분리(分離)한 탄저병균(炭疽病菌) Colletotrichum dematium에 대한 병원학적(病原學的) 연구(硏究))

  • Chung, Bong-Koo;Chang, Sun-Hwa
    • The Korean Journal of Mycology
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    • v.12 no.4
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    • pp.153-157
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    • 1984
  • In order to investigate an etiological characteristics of the pepper anthracnose fungus infected with Collectotrichum dematium, this experiment was undertaken. Isolation percent of the fungus was 55.3% from the seed sample of Jewon and 0.3% from that of Eumsung Kun, according to seed health blotter method. Although the acervulus was similar to the known two Colletotricum species, C. acutatum and C. gloeosporioides, setae was not only straightly extended above the acervulus, but also shaped as falcate conidia ranging $12{\sim}31.2{\times}2.4{\sim}4.8{\mu}m$. Acervului of the fungus were evenly scattered on the surface of pepper seed coat, and caused seedling blight after seed germination. Pre-and post-emergence seedling blight as well as foliar lesion and fruit rot was confirmed by inoculating conidial suspension. No typical anthracnose sysmptom was obtained from soybean seedlings, welsch onion and spinach seedlings upon inoculation. Therefore, this fungus is said to the undescribed form species of the fungus of pepper in Korea (Colletotricum dematium f. sp. capsicum).

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