• Title/Summary/Keyword: one-step separation

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Autopsy of Nanofiltration membrane of a decentralized demineralization plant

  • El-ghzizel, Soufian;Jalte, Hicham;Zeggar, Hajar;Zait, Mohamed;Belhamidi, Sakina;Tiyal, Fathallah;Hafsi, Mahmoud;Taky, Mohamed;Elmidaoui, Azzedine
    • Membrane and Water Treatment
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    • v.10 no.4
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    • pp.277-286
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    • 2019
  • In 2014, the first demineralization plant, using nanofiltration (NF) membrane coupled with renewable energies was realized at Al Annouar high school of Sidi Taibi, Kenitra, Morocco. This project has revealed difficulties related to the membrane performances loss (pressure increase, flux decline, poor water quality of the produced water and increase of energy consumption), as consequences of membrane fouling. To solve this problem, an autopsy of the membrane was done in order to determine the nature and origin of the fouling. The samples of membrane and fouling were then analyzed by scanning electron microscopy using a scanning electron microscope (SEM) connected with an energy dispersive X-ray (EDX) detection system and X-ray diffractometer (XRD). Moreover, three cleaning solutions (hydrochloric acid, nitric acid and sulfuric acid) were tested and assessed in a single cleaning step to find the suitable one for the fouled membrane to regain its initial permeability and performances. The analysis of the experimental results showed that the fouling layer is mainly composed of calcium carbonate (inorganic fouling). Results showed also that the permeability is improved by the hydrochloric acid cleaning (pH=3) with a cleaning efficiency of 93%. Cleaning efficiency did not exceed 75 % with nitric acid (pH=3) and 40 % with sulfuric acid (pH=3).

Structural Design and Analysis of Pico-class Satellite named STEP Cube Lab

  • Jeon, Su-Hyeon;Jang, Su-Eun;Jung, Hyun-Mo;Cha, Jin-Yeong;Oh, Hyun-Ung
    • International Journal of Aerospace System Engineering
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    • v.1 no.1
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    • pp.34-43
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    • 2014
  • The STEP Cube Lab (Cube Laboratory for Space Technology Experimental Projects) is a 1U cube satellite developed by the Space Technology Synthesis Laboratory of Chosun University to be launched in 2015. Its mission objective is twofold: to determine which of the fundamental space technologies researched at domestic universities, will be potential candidates for use in future space missions and to verify the effectiveness of the technologies by investigating mission data obtained from on-orbit operation of the cube satellite. In this paper, a structural design concept based on the 1U standard to achieve the mission objective is introduced. The validity of the design has been demonstrated by quasi-static analysis and modal analysis. In addition, a non-explosive separation device triggered by burn wire heating, which is one of the main mission payloads is introduced.

NEAR INFRARED TRANSFLECTANCE SPECTROSCOPY (NIRS) IN PHYTOCHEMISTRY

  • Huck, C.W.;W.Guggenbichler;Bonn, G.K.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.3114-3114
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    • 2001
  • During the last years phytochemistry and phytopharmaceutical applications have developed rapidly and so there exists a high demand for faster and more efficient analysis techniques. Therefore we have established a near infrared transflectance spectroscopy (NIRS) method that allows a qualitative and quantitative determination of new polyphenolic pharmacological active leading compounds within a few seconds. As the NIR spectrometer has to be calibrated the compound of interest has at first to be characterized by using one or other a combination of chromatographic or electrophoretic separation techniques such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC), capillary electrophoresis (CE), gas chromatography (GC) and capillary electrochromatography (CEC). Both structural elucidation and quantitative analysis of the phenolic compound is possible by direct coupling of the mentioned separation methods with a mass spectrometer (GC-MS, LC-MS/MS, CE-MS, CEC-MS) and a NMR spectrometer (LC-NMR). Furthermore the compound has to be isolated (NPLC, MPLC, prep. TLC, prep. HPLC) and its structure elucidated by spectroscopic techniques (UV, IR, HR-MS, NMR) and chemical synthesis. After that HPLC can be used to provide the reference data for the calibration step of the near infrared spectrometer. The NIRS calibration step is time consuming, which is compensated by short analysis times. After validation of the established NIRS method it is possible to determine the polyphenolic compound within seconds which allows to raise the efficiency in quality control and to reduce costs especially in the phytopharmaceutical industry.

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A New Chemical for the Separation of the CRT Panel Glass from its Funnel

  • Lee, Ki-Won;Byun, Ji-Young;Kim, Kyong-Tae;Oh, Jong-Kee
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.71-75
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    • 2001
  • The first step for recycling the CRT bulb is to remove frist seals between the panel and funnel. For this purpose, various kinds of methods have been used. One of those is to use the nitric acid, which is a proven technology and widely used in CRT-making industries. The process. however. has a problem of NOx generation. Such a drawback can be overcome by using a new chemical. This new chemical can remove the frit without NOx generation. This paper describes the dissolution ability of the chemical for lead and zinc oxides and the application to the separation of the CRT panel from its funnel.

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Purification of a Mosquitocidal Toxic Protein from B. thuringiensis strain H9B by Immuno-Affinity Chromatography (Immuno-Affinity Chromatography에 의한 B. thuringiensis H9B 균주의 모기살충성 내독소 단백질의 정제)

  • 김광현;배수장;이광배
    • Journal of environmental and Sanitary engineering
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    • v.12 no.2
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    • pp.59-64
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    • 1997
  • For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with protein A-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na$_{2}$HPO$_{4}$ buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.

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Performance Evaluation of Hinge Driving Separation Nut-type Holding and Releasing Mechanism Triggered by Nichrome Burn Wire

  • LEE, Myeong-Jae;LEE, Yong-Keun;OH, Hyun-Ung
    • International Journal of Aeronautical and Space Sciences
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    • v.16 no.4
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    • pp.602-613
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    • 2015
  • As one of the mission payloads to be verified through the cube satellite mission of Cube Laboratory for Space Technology Experimental Project (STEP Cube Lab), we developed a hinge driving separation nut-type holding and releasing mechanism. The mechanism offers advantages, such as a large holding capacity and negligible induced shock, although its activation principle is based on a nylon cable cutting mechanism triggered by a nichrome burn wire generally used for cube satellite applications for the purpose of holding and releasing onboard appendages owing to its simplicity and low cost. The basic characteristics of the mechanism have been measured through a release function test, static load test under qualification temperature limits, and shock measurement test. In addition, the structural safety and operational functionality of the mechanism module under launch and on-orbit environments have been successfully demonstrated through a vibration test and thermal vacuum test.

Preferential Sorption and Its Role on Pervaporation of Organic Liquid Mixtures

  • 박현채;김은영
    • Proceedings of the Membrane Society of Korea Conference
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    • 1995.04a
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    • pp.34-35
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    • 1995
  • The unique feature of pervaporation is the mass transfer from a liquid phase to a vapor phase through a non-porous polymeric membrane. When a liquid mixture is brought into contact with a membrane at one side, it is sorbed into the membrane. Due to a driving force applied across the membrane, the sotbed liquid molecules permeate through the membrane and evaporate at the downstream side of the membrane. In pervaporation the permeated species are usually removed from the downstream side under a relatively low vapor pressure, for example by evacuation with a vacuum pump. As far as this condition is fulfilled, the evaporation step can be considered to be much faster than sorption or diffusion. Hence evaporation does not contribute to permselectivity. Therefore the separation by pervaporation results from the differences in the preferential sorption of the individual components of a mixture into the membrane together with the diffusion rates through the membrane. This postulation implies that both sorption and diffusion phenomena have to be accounted for to understand the physico-chemical nature of the pervaporation separation process.

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Evaluation of a Pretreatment Method for Two-Dimensional Gel Electrophoresis of Synovial Fluid Using Cartilage Oligomeric Matrix Protein as a Marker

  • Kong, Min-Kyung;Min, Byoung-Hyun;Lee, Pyung-Cheon
    • Journal of Microbiology and Biotechnology
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    • v.22 no.5
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    • pp.654-658
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    • 2012
  • Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA. The efficient and reliable preparation of SF proteomes is a critical step towards biomarker discovery. In this study, we have optimized a pretreatment method for twodimensional gel electrophoresis (2DE) separation of the SF proteome, by enriching low-abundance proteins and simultaneously removing hyaluronic acid, albumin, and IgG. SF samples pretreated using this optimized method were then evaluated by 1DE and 2DE separation followed by immunodetection of cartilage oligomeric matrix protein (COMP), a known OA biomarker, and by the identification of 3 proteins (apolipoprotein, haptoglobin precursor, and fibrinogen D fragment) that are related to joint diseases.

An Education Model of a Nano-Positioning System for Mechanical Engineers

  • Lee Dong-Yeon;Gweon Dae-Gab
    • Journal of Mechanical Science and Technology
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    • v.20 no.10
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    • pp.1702-1715
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    • 2006
  • The increasing use of nano-positioners in a wide variety of laboratory and industrial applications has created a need for nano-mechatronics education in all engineering disciplines. The subject of nano-mechatronics is broad and interdisciplinary. This article focuses on the way nano-mechatronics is taught in department of mechanical engineering at Korea Advanced Institute of Science and Technology (KAIST). As one model of nano-positioning systems, design and experimental methodology is presented in this article. For design phase, the stiffness and resonant frequencies are found analytically and verified by using a commercial finite element analysis program. Next, for experimental phase, various tests are performed to access the performances of the designed nano-positioner, for example, sine-tracking, multi-step response and travel-range check etc. Finally, the definition of 'separation frequency' is described and some comments are discussed.

Rapid Separation of Cellular Cyclosophoraoses Produced by Rhizobium Species

  • Seo, Dong-Hyuk;Lee, Sang-Hoo;Park, Hey-Lin;Kwon, Tae-Jong;Jung, Seun-Ho
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.522-525
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    • 2002
  • A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.