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Mycelial and cultural characteristics of a new high-yield Pleurotus pulmonarius cultivar 'Santari' for bottle culture (다수성 병재배용 산느타리 신품종 '산타리'의 균사배양 및 생육특성)

  • Choi, Jong In;Lee, Yun Hae;Jeon, Dae Hoon;Gwon, Hee Min;Chi, Jeong Hyun;Shin, Pyung Gyun
    • Journal of Mushroom
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    • v.15 no.4
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    • pp.190-194
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    • 2017
  • Oyster mushrooms are one of the most popular edible mushrooms in Korea. The 'Santari' cultivar bred in this study, which belongs to the species Pleurotus pulmonarius, is a new oyster mushroom cultivar for bottle culture. It was bred by mating monokaryons isolated from 'GMPO20404' and 'Hosan'. The optimum temperature for 'Santari' mycelial growth was $26-29^{\circ}C$ on PDA medium, and the temperatures for primordium formation and for growth of the fruit body on sawdust medium were $22^{\circ}C$ and $20^{\circ}C$, respectively. It took 34 days to complete the spawn run, 3 days to form primordia, and 3 days to finish fruit body growth in the bottle culture. The fruit body pilei were round in shape and brownish, whereas the stipes were long, thick, and white. The yield per bottle of 'Santari' was 172 g/1,100 mL, which was 43% higher than that of the reference cultivar ('Hosan'). The springiness, cohesiveness, gumminess, and brittleness of the stipe tissue were 87%, 82%, 193 g, and 16 kg, respectively. These physical property values of 'Santari' were lower than those of the control cultivar, except for the cohesiveness.

Development of CRS-Korea II and its Application to Setting the Priority of Toxic Chemicals for Local Provinces (개선된 화학물질 우선순위 선정 기법(CRS-Korea II)과 그 활용을 통한 지역별 유독물 우선순위의 도출)

  • Choi Seung Pil;Park Hoa Sung;Lee Dong Soo;Shin Yong Seung;Kim Ye Shin;Shin Dong Chun
    • Environmental Analysis Health and Toxicology
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    • v.20 no.4 s.51
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    • pp.311-325
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    • 2005
  • A chemical ranking and scoring system, CRS - Korea, has recently been developed and proposed to use to prioritize on a screening level the toxic chemicals for monitoring and risk assessment. As CRS-Korea requires rigorous assessments prior to its wide application, an assessment was conducted in this study by examining the contribution of individual parameter score to the final chemical score or ranking. The sensitivity of the system to the default values for various parameters of missing data was also tested. The chemical ranking/score was round to depend primarily on the score of a single parameter, i. e., the chemical release, while toxicity scores show little correlation with the priority Further analysis indicated that the dominating effect of the chemical release results from i) its multiplicative relationship with the other two exposure parameters (biodegradation and persistent) and ii) the fact that a maximum score of 10 was assigned to the chemical release parameter while 5 was assigned for all others. AE the fraction of the data that are missing exceeded $70\%$ for various toxicity parameters at compared to less than $10\%$ for exposure parameters, the sensitivity of the ranking to the default value was not significant (rank correlation coefficient = 0.98) for toxicity parameters. Bated on this assessment, an improved CRS system (CRS - Korea II) was proposed in which the impact of the chemical release was properly adjusted by changing the multiplicative relationship to additive one and the maximum score to 5. Chemical priority was derived for each of 16 provinces by using CRS-Korea II. The chemical priority was found to significantly vary among the provinces. It was concluded that not only the national chemical priority but the local chemical priority should be taken into account in setting the nationwide chemical monitoring and risk assessment strategy.

Studies on the Digestion of Beef by Ficin Treatment (Ficin 처리 우육의 소화에 관한 연구)

  • Kim, Jung-Sook;Kim, Jun-Pyong
    • Applied Biological Chemistry
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    • v.30 no.3
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    • pp.210-218
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    • 1987
  • In the previous report, we isolated and purified one of tendering enzyme 'ficin' from fig latex. In this study, various crude ficin concentrations and reaction time were employed to investigate the contents of free amino acids and other free nitrogen compounds after the treated with beef round muscle. 1. Free amino acids contents increased with the increase of temperature and time during the aging of beef at $1^{\circ}C$ and $8^{\circ}C$, and the increasing rate was remarkably high when fresh beef was treated with ficin. In the case of ficin treatment after various cooking, steaming showed the highest increase in free amino acid contents among three cooking methods such as boiling, steaming and pan broiling. The increased amounts of free amino acids in three groups-aging beef at $1^{\circ}C$ for 3 days, fresh beef treated with ficin(0.1%, 2hrs) and beef treated with ficin(0.1%, 2hrs) after cooking were 13%, 293% and 137% respectively. In contrast to aging group, the amount of free amino acids in other two groups treated with ficin was superiorly increased. 2. The amounts of total free nitrogen, free non-protein nitrogen and $NH_3-nitrogen$ increased with the increase of temperature and time during the aging of beef at $1^{\circ}C$ and $8^{\circ}C$, and the increasing rate was remarkably high in fresh beef treated with ficin. In the case of ficin treatment after cooking, steaming gave larger amount of total and non-protein nitrogen than other two cooking, e.g. boiling and panbroiling. The increasing rate of nonprotein nitrogen to the total nitrogen of fresh beef treated with ficin(0.1%, 2hrs) was 75 times greater than that of aging fresh beef at $1^{\circ}C$ for 3 days.

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Suppressive Activity of Extract of Termialia chebula Retz. on Hepatic Fibrosis (가자(Terminalia chebula Retz.) 추출물의 간섬유화 억제활성)

  • Lee, Hyun-Sun;Koo, Yun-Chang;Lee, Kwang-Won
    • Korean Journal of Food Science and Technology
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    • v.41 no.5
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    • pp.597-601
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    • 2009
  • Activation of hepatic stellate cells (HSCs) is known to be responsible for hepatic fibrosis and cirrhosis. When round-shape quiescent HSCs go to activation by liver injury, production of extracellular matrix is increased, and its shape becomes myofibroblast-like shape. The activated HSCs are characterized by the high rate of proliferation and the increased production of extracellular matrix. One way of the regeneration of activated HSCs is an apoptosis induction followed by removing the activated myofibroblast-like cells. The effect of extract of Terminalia chebula Retz. (TCE) on cytotoxicity was evaluated using the rat primary hepatocyte, HepG2 and T-HSC/Cl-6 by incubating these cells with TCE up to the dose of $1,000{\mu}g/mL$. At the maximum dose of TCE, no cytotoxicity was found on primary hepatocyte and HepG2, but cytotoxic effect of TCE was found on activated HSCs, and T-HSC/Cl-6 in a U-shaped dose-response manner with the highest effect at $500{\mu}g/mL$ of TCE. Finally, we confirmed the occurrence of apoptotic cell death by annexin-V/PI double staining. The population of annexin-V positive cells was increased in a dose dependent manner.

Development of the Speed Limit Model for Harbour and Waterway(I) - Considerations Discrimination for Speed Limit Decision - (항만과 수로의 제한속력 설정 모델 개발에 관한 연구(I) - 제한속력 설정을 위한 고려요소 식별 -)

  • Kim, Deug-Bong;Jeong, Jae-Yong;Park, Jin-Soo;Park, Young-Soo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.21 no.2
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    • pp.171-178
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    • 2015
  • This research is the first research on developing the speed limit select model and also it is the result of the research on the importance of each element and consideration factors when selecting the speed limit. For the consideration factor discrimination and calculation of the importance, the delphi method and AHP method was used. The delphi survey was processed through third round survey, 5 high consideration factor(Level 1) and 23 low consideration factor(Level 2) was discriminated. During the process of the third delphi survey, when the CVR cost was in the range between 0.4~1.0 it was treated as the consideration factor when selecting the speed limit and less than 0.4 cost was eliminated. In the process of the second delphi survey, 33 consideration factors were discriminated but was reordered into 23 categories through the third survey. Based on the 23 categories earned through the third delphi analysis, the AHP survey was processed. The result of the AHP survey was that out of the importance of the 5 high consideration factor(Level 1), the traffic condition was evaluated as the number one factor and the vessel condition, waterway condition, environment condition, supporting condition and etc. conditions were evaluated following the traffic condition. Out of the 23 low consideration factor(Level 2) consideration, the visibility was evaluated to be the first important and the performance of the vessel steering, objective factors within the harbor, amount of traffic and density, distance between the passing vessel, speed of the steering capacity and tidal current were the following evaluated factors.

Exploring the Success Factors of K-POP Globalization: Utilizing the VRIO Model (K-POP의 세계시장 진출 성공요인 분석: VRIO 모형을 중심으로)

  • Shin, Dong-Seok;Nam, Sung-Jip;Nam, Myung-Hyun
    • Journal of Distribution Science
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    • v.13 no.2
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    • pp.55-62
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    • 2015
  • Purpose - This study aims to investigate the success factors pertaining to K-POPs from an analysis of the internal business environment. Much research has investigated Korean Moves or how to popularize them. The research mainly focused on aspects of Korean Moves. However, few studies have attempted to examine Korean Moves or K-POPs from a managerial viewpoint. The current research tries to investigate the success factors of K-POP from strategic perspectives, specifically utilizing internal resource based view perspectives. It differentiates itself by looking at the competitiveness of K-POP from the internal resources. Research design, data, and methodology - In the entertainment industry, where creativity is heavily stressed, competitiveness is often regarded within the organization as a form of intangible asset, knowledge, or technology that is often related with the organization's personnel. Some research has tried to reveal the competitiveness of K-POP using Porter's competitiveness of nations framework. Others utilize the adapted model of Porter's structure. However, these models only look at the outside environment, and not inside a firm's resource, knowledge, or capabilities. This research utilizes the VRIO model to examine the internal resources and capabilities of K-POP producers. The model measures whether a firm's internal resources and capabilities are valuable, rare, difficult to imitate by competitors, or organizable. The research covered businesses whose yearly revenue exceeds $10 Million in music planning and recording in South Korea. There were only thirteen such companies (one percent of the total population). Of these, companies for whom 20 percent or more of the sales revenue comes from the abroad are targeted. Only seven are selected and these participated in the research. In order to find a firm's internal resources, we conducted qualitative research methodology. Their business names and persons who participated in this research are not revealed due to case sensitive issues. Instead, we use unrelated initials for their names and their statements. Results - From the in-depth interview with top-tier K-POP producers and managers, the current research tried to identify resources and capabilities that helped to strengthen their competitiveness. These resources and capabilities are sought from the scope of the VRIO model, which looks at the internal resources and capabilities from the scope of value, rarity, imitability, and organization. Interviews with the top tier producers and managers reveal the internal success factors of K-POPs. We conclude that these resources and capabilities are from internally accumulated producing know-how, unique managing (training) system, and outstanding all-round entertainment capabilities of the performers. Conclusions - These results indicate that the core resources and capabilities of K-POP are robust. It will take a significant amount of time and money to imitate for followers, because these resources and capabilities are the result of time investment and are embedded into producers' and performers' know-how. Taking Luo (2000)'s argument, K-POP is in the second stage of the globalization process, which is configuring and allocation resource capabilities to a global scope.

Integumentary Ultrastructure of the Blenny, Pholis nebulosa (Teleostei: Pholidae) (베도라치 (Pholis nebulosa) 피부계의 미세구조)

  • LEE Jung Sick;An Cheul Min;HUH Sung-Hoi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.2
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    • pp.148-152
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    • 2000
  • To provide basic information on the integumentary system of the blenny, Pholis nebulosa, ultrastructures of epidermal and dermal layer were examined by means of the light and transmission electron microscope. The skin of the blenny consisted of epidermal and dermal layer. Epidermal layer consisted of supporting cell and unicellular gland. The supporting cells were classified into superficial cell, intermediated cell and basal cell, and the gland cells were classified into mucous secretory cell and club cell. The cytoplasm of supporting cells was divided into cortex and medullar part. In the cortex and medullar part, microfilaments and cell organelles were well developed, respectively. Superficial cell of epidermal layer was cuboidal and contained nucleus of horseshoe shape. Intermediated cell had a nucleus of irregular form and the electron density was higher than the other supporting cells, Basal cell was columnar, but nucleus was situated in the upper cytoplasm. Cell organelles of the basal cell were poor than the other supporting cells, but membrane interdigitations were well developed. The cytoplasm of mucous secretory cell had a well-developed ovoid secretory granules, which reacted to red with AB-PAS reaction. The club cell had a we31-developed round secretory granules and endoplasmic reticulum. figment cells were classified into two type. The one contained pigment granules of electron dense, and the other contained reflecting platelets. The cytoplasm of fibrocyte had n well developed rough endoplasmic reticulum.

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The Effect on the Marine Water and Sediment Quality by the Use of Nano-S 1. Result of Water Tank Experiment (Nano-S가 해양수질 및 저질에 미치는 영향에 관한 연구 1. 실내수조실험 결과)

  • Cho, Hyeon-Seo;Cho, Chon-Rae;Jang, Young-Nam
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.8 no.3
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    • pp.158-163
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    • 2005
  • The purpose of this study was to observe the effect on the marine environment by the use of Nano-S. Nano-S was made to apply to improve the red tide bloom. The experiment was performed at round tank with volume of 180 L. Each tank was filled with an aggravated sediment about $14{\pm}1cm$ hight and sea water. The water flow-rate of tank was established on the rate of 6.25 L/hr. Sea water level was fitted to 40 cm, therefore the filled water was about 150 L. The sediment was stabilized during one week. Then the Nano-S and the red mud were added into each tank 0 kg(control), 1 kg(tank A), 2 kg(tank B), 5 kg(tank C) and 10 kg(tank D) each other. The quantity was fulfilled with 0 kg(control), 2.75 kg(tank A), 5.51 kg(tank B), 13.77 kg(tank C) and 27.55 kg(tank D) per square meter of sediment. The experiment was performed during 30 days. Water and sediment samples were collected from each tanks on the before 1hour and after 1, 3, 6, 12 hour and 1st, 3th, 5th, 7th, 10th, 15th, 30th day of the experiment period. The change of water and sediment quality was analyzed before and after applying the Nano-S and the red mud.

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Conservation and Pedestal Stability Estimation of the Bukji-ri Stone Pensive Bodhisattva of Bonghwa (봉화 북지리 석조반가상의 보존 및 받침대 안정성 평가)

  • Chae, Woomin;Jang, Minkyeong;Yi, Younghee;Hwang, Hyunsung
    • Conservation Science in Museum
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    • v.17
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    • pp.85-100
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    • 2016
  • The Bukji-ri Stone Pensive Bodhisattva of Bonghwa in the collection of Kyungpook National University Museum was transported to the National Museum of Korea for display in a special exhibition('Masterpieces of Early Buddhist Sculpture 100 BCE - 700 CE') and therefore underwent conservation ahead of the exhibition's start date. The stone sculpture had visibly encrusted surface dirt, granular disintegration and fissures upon arrival. Notably, a crack running obliquely across its lower half rendered the object unable to support its own weight without a pedestal, so one was created in order to maintain the sculpture in an upright position while on exhibition. The sculpture was further examined using a polarizing microscope and a stereoscopic microscope. SEM-EDS resulted in petrographic analysis of the stone's mineral composition and identification of its surface contaminants. Polarizing light microscopy confirmed biotite granite as the main mineral component of the object. Several urethane resins cast in round cross-sections were inserted into the newly made pedestal and stability tests were perform to measure the frictional force of the resins. An additional test was performed to compare urethane resin and epoxy resin, with results showing urethane to have a higher coefficient of friction. Utilizing a pedestal with urethane resin effectively ensured the stability of the Bukji-ri Stone Pensive Bodhisattva of Bonghwa during the aforementioned exhibition.

Breeding of Artificial Autotetraploids from Cold Hardness Lines of Yongchonppong and Yeongbyonppong (내동성계 재래뽕 용천뽕과 영변뽕의 동질4배체 육성)

  • 박광준
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.93-99
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    • 1996
  • By treatment(dropping) of 0.1~0.4% colchicine solution on the sprouts of winter buds of hard wood cutting slips for 4~5 days, two lines of artificial autotetraploids from Yongchonppong and one line from Yeongbyonppong were bred and the important cultivative characteristics of those new lines were as follows. The greentip sprouting stage of the new bred lines in spring season is later than the parental varieties by two days, but growth speed of the new lines after sprouting was faster than that of the parental varieties reaching the same level development with the parental varieties at the fifth leaf sprouting stage to be mid varieties same as the origins. The leaf shape of the new bred lines was wide round and the petioloes were long and thick. The thickness of leaf was thicker than the parental varieties by 17-33% and single leaf weight was heavy. The leaf area weight increased by 21-31% and the content of chlorophyll was also higher by 11-33%. With all the characteristics, the new breds produced good quality of leaves. The length and number of branches were shorter and less, respectively, than the parental varieties, but the internode length was either same or longer than the parents. Looking at the characteristics, the constitution of shoots was slightly inferior to the parental varieties. The cold hardness expressed by the death top rate of Sawonppong 23 and Sawonppong 24 was same level as that of Yongchonppong, but Sawonppong 25 was stronger than Yeongbyonppong in it with a high infection rate of dwarf disease. The productivity was lower than the parental varieties, but young shoot rate to shoot and branch and the ratio of leaf to young shoot were higher than the parental varieties. The fertility of Sawonppong 23 and Sawonppong 24 was comparatively high with 62% of cross success, but that of Sawonppong 25 was low with 23.9% of cross success.

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