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Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects

  • Um, In-Woong;Hwang, Suk-Hyun;Kim, Young-Kyun;Kim, Moon-Young;Jun, Sang-Ho;Ryu, Jae-Jun;Jang, Hyon-Seok
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.42 no.2
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    • pp.90-98
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    • 2016
  • Objectives: The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2. Materials and Methods: Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (${\mu}CT$) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals. Results: Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a twofold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The ${\mu}CT$ analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks. Conclusion: Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.

Uptake and Accumulation of Arsenate on Lettuce (Lactuca sativa L.) Grown in Soils Mixed with Various Rates of Arsenopyrite Gravel (유비철석 입자 혼합 토양내 상추(Lactuca sativa L.)의 비소이온 흡수와 축적)

  • Shim, Ho-Young;Lee, Kyo-Suk;Lee, Dong-Sung;Jeon, Dae-Sung;Shin, Ji-Su;Kim, Soo-Bin;Cho, Jin-Woong;Chung, Doug-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.532-538
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    • 2014
  • Arsenic (As) is nonessential element toxic to plants. In Korea little is not only known about the extent of actual anthropogenic sources and inputs of arsenic to the agricultural land which plays a active role as a sink, but also systematic research on arsenic as an toxic element entering the food chain via the soil-plant pathway has not been investigated in the fields and greenhouses besides in few places of abandoned mining sites. Therefore, it is important to focus on the effect of As-contaminated soils on As uptake and biomass production of lettuce plants. In this study, As concentrations in the soil and accumulation of As in lettuce transferred by As uptake from soils were investigated. To do this, soil which was mixed with various rates of arsenopyrite gravels containing arsenic from 0 to 100% was packed into a round plastic pot. Then, 10 days old vegetable crops of chinese cabbage and lettuce after germination were transplanted into a pot. Growth of lettuce was observed for four weeks with one week interval. All experiments were done by triplicate. The results showed that the growth rates for number of leaves, width and length of the crop plants were retarded with increasing amount of gravel mixed due to increasing bioavailable amount of arsenate with increasing rate of gravel in soils. With these results, we conclude that the bioavailable amount of arsenate can influence the growth of lettuce.

An Exploratory Study on the Globalization of Korean Cuisine through the Application of Multicultural Cuisine (다문화적 조리 접근법을 통한 한식 세계화에 대한 탐색적 연구)

  • Jung, Yung-Woo
    • Culinary science and hospitality research
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    • v.16 no.2
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    • pp.170-184
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    • 2010
  • As Korean cuisine is one of our cultural assets and has global potential, it needs to be better marketed and known to the world. However, there is a lack of research examining what to and how to globalize Korean cuisine practically. Therefore, the purpose of this study is to suggest fundamental reasons and ways to globalize Korean cuisine from a multicultural point of view. A Delphi method was selected because the study is suitable for a structured group of experts instead of unstructured groups or individuals. A panel of experts were selected from two groups, Korean experts and international experts. This research established the characteristics of Korean foods, the difficulties of Korean cooking methods and the incorporation or substitution of Western ingredients in two rounds. A panel of experts suggested strategies for globalizing Korean cuisine in the third round. As a result, meaningful solutions were suggested such as increasing the global profile and knowledge of Korean cuisine from 18 experts out of 23, developing various cooking methods for Korean-cuisine having distinctively Korean characteristics from 17 experts out of 23, developing recipes that are compatible with equipment and appliances in a western kitchen, and considering the differences in taste and texture(mouth-feeling) between Korean and the Western people.

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A Study on the Changes of the Plan Type of Protestant Church Architecture in Korea - Focused on the distinction between the sexes(男女有別) and the seat position of the sexes - (한국 개신교 교회건축의 평면형태 변천 연구 - '남녀유별' 관념과 회중석의 남녀 위치를 중심으로 -)

  • Lee, Hee-Jun;Yoon, In-Suk
    • Journal of architectural history
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    • v.14 no.3 s.43
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    • pp.129-148
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    • 2005
  • The plan type of Korean protestant church architecture underwent changes as following five stages according to the changes of the distinction between the sexes. First stage - At the beginning of missionary work in Korea, Koreans generally worshiped in missionary houses which were traditional Korean styles. The plan type of these houses was a simple '-' figure. At that time, men and women worshiped separately because of the idea of the distinction between the sexes. They either worshiped in different places at the same time or in the same place at the different time. Second stage - At this stage, men and women started to worship together in the same place. At the beginning of this stage, men sat in the front of the chapel on a rostrum, and women sat behind the men. The plan type of the chapel was a simple '-' or a rectangular figure. Later, they sat separately on the right and left side. There are the visual interceptions which were set up between them. As the number of church members increased rapidly, a new type of church architecture appeared. It was a 'ㄱ' figure. At that time the entrances were separated by the sexes. Third stage - At this stage, the visual interceptions disappeared as the idea of the distinction between the sexes became weak. As new churches had been constructed by the plan type of rectangular figure, the visual interceptions weren't set up anymore. Fourth stage - At this stage, the separated entrances were unified as one. But the arrangement of their seats didn't change because of the old idea of the distinction between the sexes. Fifth stage - In the final stage, the plan type of the church architecture was not determined by the idea of the distinction between the sexes but social, economical, technological facts and the influence of the foreign architecture. At this stage, new and various kinds of the plan type appeared such as the fan, round, squared, or oval figure as well as the rectangular figure. Men and women were not classified anymore. They started to sit and worship together in the same place. Also, when men and women sat separately from side to side because of the Idea of the distinction between the sexes, men sat on the right and women sat on the left side of rostrum. It didn't applied Confucian ideas but the idea of protestant church; protestant churches have the idea that the right side is more important but it does not in Confucianism.

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Study on the histomorphometry of guided bone regeneration using automated image analysis system (자동 영상분석 계기를 이용한 골 유도재생능력의 분석에 관한 조직계측학적 연구)

  • Kim, T.I.;Ku, Y.;Rhyu, I.C.;Chung, C.P.;Han, S.B.;Choi, S.M.;Son, S.H.
    • Journal of Periodontal and Implant Science
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    • v.26 no.3
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    • pp.771-778
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    • 1996
  • The assessment of alveolar bone changes on dental radiographs to indicate progression of periodontal diseases or healing response to therapy is routine procedure. However, the diagnostic accuracy in detecting small alveolar bone changes is very limited. Recently, guided bone regeneration therapy is popular, but the quantification of new bone is somewhat difficult with conventional evaluation method. To quantificate the amount of new bone, various evaluating methods have been introduced including histomorphometry, radiomorphometry, biochemical analysis, X-ray probe microanalysis, scanning electron microscope backscatter method. In this study, guided bone regeneration using resorbable membrane with & without PDGF-BB is quatificated through histomorphmetry to evaluate the efficacy of histomorphometric analysis. 4 beagle dogs and 8 Sprague-Dawley rats were selected as experimental animals. In beagle dog experiment, $4{\times}4mm$ Class II defects were created in maxillary both second premolars, and biodegradable membrane containing PDGF-BB(experimental group) were covered over one defect, and same membrane without PDGF-BB(control group) were covered over the other defect. At 2 weeks, 5 weeks after surgery, each beagle dogs were sacrificed, and the tissues were treated by undecalcified fixation. In Sprague-Dawley rat experiment, 5mm round defect were created in temporal bone, the same membranes were covered on the defects. At 1 week, 2 weeks after surgery, each rats were sacrificed, and undecalcified fixation were taken. After grinding tissue specimen, we analyse them histomorphometrically using image analysis system. In beagle dog 2 weeks specimens, new bone formation area were $0.03123mm^2$ in experimental group,and $0.03012mm^2$ in control group. At 5 weeks specimens, $0.15324mm^2$ in experimental group, and $0.09123mm^2$ in control group. In Sprague-Dawley rat specimens, new bone fomation area were $0.20448mm^2$ in 1 week experimental group, $0.03604mm^2$ in 1 week control group. At 2 weeks specimens, $0.46349mm^2$ in experimental group, $0.17741mm^2$ in control group. The results indicated that histomorphometric analysis of new bone formation using image analysis system is very effective quantification method to evaluate the efficacy of treatment modalities.

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The Effect of UR on Chestnut Growers (우루과이 라운드(UR)가 밤 재배농가에 미치는 영향)

  • Choi, Kwan;Han, Sang Yeol;Woo, Tae Myung;Sung, Kyu Chul
    • Journal of Korean Society of Forest Science
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    • v.81 no.3
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    • pp.255-262
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    • 1992
  • Urguay Round(UR) has lots of implication in the forest product market as well as the other sectors of the economy. Chestnut, one of the major forest product in Korea, would be affected by free trade resulting from the agreement on UR. To establish effective policy measures dealing with negative effects of free trade, if any, the effect of UR on producers should be figured out. In this contest, the purposes of this study are (1) estimating the demand, supply and its price functions of this market and (2) forecasting the effect of UR on growers. Using econometric method, demand, supply and price function of this market are estimated. The total amount of yearly money loss of growers due to free trade from 1992 to 2001 are estimated for four different scenarios. In each scenario, it is assumed that the tariffication reduction is 30%, 40%, 50% and 90%. Yearly money loss of chestnut growers at the year 2001 are forecasted such as 14 billion won, 18 billion won, 24 billion won and 25 billion won for the rate of tariffication reduction of 30%, 40%, 50%, and 90%, respectively.

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Effects of Squalene on Mouse Liver Toxicity with Cadmium (Cadmium으로 유발된 흰쥐 간독성에 대한 스쿠알렌 효과)

  • Kim, Jong-Se;Yoon, Jung-Sik
    • Applied Microscopy
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    • v.30 no.2
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    • pp.141-152
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    • 2000
  • This study aims to demonstrate the effect of squalene (SQ), one of the natural chelator, on the ultrastructural changes in the mouse liver caused by $CdCl_2$. A total of 40 healthy ICR that weighted 30 gm $({\pm}2gm)$ was used for experiment. The experimental group was divided into two groups; group A and B. The group A administrated $CdCl_2$ (4.0 mg/kg) to the intraperitoneal. The group B administrated $CdCl_2$ (4.0 mg/kg) to the intraperitoneal treated with SQ (180 mg/kg, 2 time/day). Each group was observed at 24, 48, 72, 96 hours after injected $CdCl_2$. The results were as follows: 1. Group A Nuclear membrane was observed very irregular at the 24 hours and keep rounded-shape from 48 hours. Mitochondria were observed destruction of inner cavity to the 72 hours and some showed inner cavity destruction at 96 hours. RER (rough endoplasmic reticulum) showed the dilation of inner cavity all the around and reformed typical lamellae from 72 hours. Lysosome were observed in the cytoplasm from 24 hours. From 72 hours, glycogen showed over cytoplasm. 2. Group B Nuclear membrane was observed regular at overall the time. Mitochondria showed normal shapes (round, rod) at overall the time. To 48 hours, inner cavity of rER dilated and destructed lamellae. But from 72 hours, observed typical lamellae of rER Lysosome were observed from 24 hours. And glycogen showed from 24 hours. These results suggest that squalene attenuates the toxic effect of the $CdCl_2$ in the mouse liver.

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Relationship between Degree of Grain Shedding and Histological Peculiarities of Abcission Region of Red Rice(Oryza sativa L.) Collected in Korea (국내 자생벼 계통의 탈립성과 이층조직의 특성)

  • 임원재;최광귀;진일두
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.3
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    • pp.239-247
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    • 1990
  • In order to investigate the degree of grain shedding of red rices collected in Korea, breaking tensile and breaking bending strengths of 269 varieties; 82 Korean and 100 foreign red rices and 87 Korean cultivars including 26 native varieties, 30 Japonica-Indica hybrids and 31 Japonica type varieties, were measured at harvest time. Also, histological characteristics of abscission region between spikelet and pedicel were observed. The breaking tensile and breaking bending strengths of both Korean and foreign red rices were weaker than those of Japonica-Indica hybrids which were known as easily shedding varieties in Korea. Abscission layers were observed in the majority of Korean red rices except for 5 varieties, and those of all varieties belonging to both long grain types and 'Sare's were cracked completely at harvest time, while those of round grain types were not cracked except one variety. The breaking tensile and breaking bending strengths of the tested varieties having cracked abscission layer were weaker than those of non-cracked ones. Both strengths of breaking tensile and breaking bending were positively correlated with diameter of supporting zone and thickness of sclerenchyma tissue surrounding central vascular tissue of suporting zone at 0.1% significant level, respectively.

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Review of the Current Forage Production, Supply, and Quality Measure Standard in South Korea

  • Kim, Jong Duk;Seo, Myeongchon;Lee, Sang Cheol;Han, Kun-Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.3
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    • pp.149-155
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    • 2020
  • Cattle feeding in South Korea has been heavily dependent on domestically produced rice straw and imported grain. Around 42% of domestically produced rice straw is utilized for forage, and the remainder is recycled to restore soil fertility. Approximately 35% of round baleages were made with rice straw. However, higher quality hay is desired over rice straw. Due to increasing stockpiles of rice, there has been an economic burden on the government to store the surplus; therefore production of annual forage crops in rice fields has been further promoted in recent years. Hay import from the USA currently constitutes more than 80% of total imported hays. The main imported hays are alfalfa (Medicago sativa), timothy (Phleum pretense), and tall fescue (Festica arundinacea). The estimated forage required for cattle feeding was approximately 5.4 million MT in 2016. Domestically produced forage sates only 43% of that value, while low quality rice straw and imported hay covered the rest of demand by 33% and 20%, respectively. As utilization of domestically produced forage is more desirable for forage-based cattle production, long-term strategies have been necessary to promote domestic production of high quality baleage. One such strategy has been utilizing the fertile soil and abundance of fallow rice fields of western region of S. Korea to produce forage crops. Italian ryegrass (Lolium multiflorum) is the most successfully produced winter annual in the region and is approximately 56% of the total winter annual forage production. Forage sorghums (Sorghum bicolor), sorghum × sudangrass hybrids, and hybrid corn (Zea mays) produce a substantial amount of warm-season forage during summer. Produced forage has been largely stored through baleage due to heavy dew and frequent rains and has been evaluated according to S. Korea's newly implemented baleage commodity evaluation system. The system weighs 50% of its total grading points on moisture content because of its importance in deliverable DM content and desirable baleage fermentation; this has proved to be an effective method. Although further improvement is required for the future of forage production in South Korea, the current government-led forage production in rice fields has been able to alleviate some of the country's shortage for quality hay.

Evaluation of Nondestructive Evaluation Size Measurement for Integrity Assessment of Axial Outside Diameter Stress Corrosion Cracking in Steam Generator Tubes (증기발생기 전열관 외면 축균열 건전성 평가를 위한 비파괴검사 크기 측정 평가)

  • Joo, Kyung-Mun;Hong, Jun-Hee
    • Journal of the Korean Society for Nondestructive Testing
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    • v.35 no.1
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    • pp.61-67
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    • 2015
  • Recently, the initiation of outside diameter stress corrosion cracking (ODSCC) at the tube support plate region of domestic steam generators (SG) with Alloy600 HTMA tubes has been increasing. As a result, SGs with Alloy600 HTMA tubes must be replaced early or are scheduled to be replaced prior to their designed lifetime. ODSCC is one of the biggest threats to the integrity of SG tubes. Therefore, the accurate evaluation of tube integrity to determine ODSCC is needed. Eddy current testing (ECT) is conducted periodically, and its results could be input as parameters for evaluating the integrity of SG tubes. The reliability of an ECT inspection system depends on the performance of the inspection technique and abilty of the analyst. The detection probability and ECT sizing error of degradation are considered to be the performance indices of a nondestructive evaluation (NDE) system. This paper introduces an optimized evaluation method for ECT, as well as the sizing error, including the analyst performance. This study was based on the results of a round robin program in which 10 inspection analysts from 5 different companies participated. The analysis of ECT sizing results was performed using a linear regression model relating the true defect size data to the measured ECT size data.