• Korean journal of communication and information
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• v.66
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• pp.5-27
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• 2014

The past, present, and future of online journalism in Korea are inextricably bound up with the portal site news services. In traditional journalism, finite news media took hold on news consumption completely because of performing news production and distribution simultaneously. Since then, in the age of multi-media and multi-channel, the audience selection has become more important because of greatly increasing news media and then exploding news production. In online journalism, the news distribution have emerged as the most important field because of the intensive consuming the portal site news services making use of various news easier, the characteristic of news consumption focusing on not news media brands but news media contents, etc. Therefore, the news present landscape of Korean journalism oriented news distribution has been completed by that the portal site news services became the symbol of online journalism. That the portal site news services performing only news distribution have more influence than the paper based online newspapers and Internet based online newspapers producing news means the change of media power. By this, a conflict between news media and portal site news services appears at all times.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2012.11a
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• pp.279-280
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• 2012

The Construction Standard Production Unit(CSPU) has been used for the standard cost estimate in public and private construction projects. However, It is questionable if the reliability and/or authenticity of the system is acceptable due to the lack of consistent enactment and/or revision procedures. This study identifies informational conditions and issues relative to using daily work report as a data collection method for enacting and/or revising CSPU system, and establishes the measures for improving the daily work report. This study aims on the information constitution of daily job-site report, daily manpower report, wages register for specialty contractors. According to the research results, most of necessary data were included in a daily job-site report. In conclusion, it is investigated that data of daily manpower report and wage register need to be included in a daily job-site report for understanding the current state of worker in the future.

• Journal of Korean Society of Water and Wastewater
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• v.29 no.5
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• pp.575-581
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• 2015

Although disinfection in drinking water treatment plants provides a safer water supply by inactivating pathogenic microorganisms, harmful disinfection by-products may be formed. In this study, the disinfectant, chlorine, was produced on-site from the electrolysis of salt (NaCl), and the by-products of the disinfection process, bromate and chlorate, were analyzed. The provisional guideline levels for bromate and chlorate in drinking water are $10{\mu}g/L$ and $700{\mu}g/L$, in Korea, respectively. Bromide salt was detected at concentrations ranging from 6.0 ~ 622 mg/kg. Bromate and chlorate were detected at concentrations ranging from non-detect (ND) ~ 45.3mg/L and 40.5 ~ 1,202 mg/L, respectively. When comparing the bromide concentration in the salt to the bromate concentration in the chlorine produced by salt electrolysis, the correlation of bromide to bromate concentration was 0.870 (active chlorine concentration from on-site production: 0.6-0.8%, n=40). The correlation of bromate concentration in the chlorine produced to that in the treated water was 0.866.

• Archives of Pharmacal Research
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• v.28 no.3
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• pp.274-279
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• 2005

Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing the development and progression of disease in experimental models of autoimmunity. In this study, the effects of myricetin, a naturally occurring flavonoid present in fruits, vegetables and medicinal herbs, on the production of IL-12 were investigated in mouse macrophages stimulated with lipopolysaccharide (LPS). Myricetin significantly inhibited the LPS­induced IL-12 production from both primary macrophages and the RAW264.7 monocytic cell-line in a dose-dependent manner. The effect of myricetin on IL-12 gene promoter activation was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect was mapped to a region in the IL-12 gene promoter containing a binding site for NF-${\kappa}B$. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-${\kappa}B$ site, which significantly decreased upon addition of myricetin, indicating that myricetin inhibited IL-12 production in LPS-activated macrophages via the down­regulation of NF-KB binding activity.

• Journal of Microbiology and Biotechnology
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• v.33 no.3
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• pp.410-418
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• 2023

Bacilysin is a dipeptide antibiotic composed of L-alanine and L-anticapsin produced by certain strains of Bacillus subtilis. Bacilysin is gaining increasing attention in industrial agriculture and pharmaceutical industries due to its potent antagonistic effects on various bacterial, fungal, and algal pathogens. However, its use in industrial applications is hindered by its low production in the native producer. The biosynthesis of bacilysin is mainly based on the bacABCDEF operon. Examination of the sequence surrounding the upstream of the bac operon did not reveal a clear, strong ribosome binding site (RBS). Therefore, in this study, we aimed to investigate the impact of RBS as a potential route to improve bacilysin production. For this, the 5' untranslated region (5'UTR) of the bac operon was edited using the CRISPR/Cas9 approach by introducing a strong ribosome binding sequence carrying the canonical Shine-Dalgarno sequence (TAAGGAGG) with an 8 nt spacing from the AUG start codon. Strong RBS substitution resulted in a 2.87-fold increase in bacilysin production without affecting growth. Strong RBS substitution also improved the mRNA stability of the bac operon. All these data revealed that extensive RBS engineering is a promising key option for enhancing bacilysin production in its native producers.

• Preventive Nutrition and Food Science
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• v.6 no.1
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• pp.79-81
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• 2001

Deoxynucleoside kinases exist as heterodimeric pairs specific for deoxyadenosine/deoxyguanosine kinase (dAK/dGK) and deoxyadenosine/deoxycytidine kinase (dAK/dCK). The aspartic acid-84 in dGK was mutated to alanine, asparagine and glutamic acid by site-directed mutagenesis. The mutation resulted in a drastic decease in dGK activity compared to the unmodified cloned enzyme while it increased production of dAK activity. The mutated dak/dgk genes, which synthesize tandem deoxyadenosine/deoxyguanosine kinase, were inserted back to the Lactobacillus acidophilus and Lactococcus lactis by electroporation to determine the effect of site-directed mutation of he enzymes on the microbial growth. However, no significant change was observed in cell growth and lactic acid production between wild type and mutant lactic acid bacteria.

• Journal of Energy Engineering
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• v.23 no.3
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• pp.102-115
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• 2014

In the Ulleung basin in the East Sea of Korea, it is assumed that huge amounts of gas hydrate(GH) are buried. In 2010, drilling operation was performed at the 2nd Ulleung Basin Gas Hydrate Drilling Expedition(UBGH2) to designate a site for field production test. In this study, based on the field production test site model reflecting geological properties, GH dissociation flow interpretation is analyzed and sensitivity analysis is performed to gain understanding of production behavior properties following bottomhole pressure(BHP) variation by using the numerical simulation. The results of this study provide a basis for the preliminary analysis of field production test site.

• Advances in environmental research
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• v.3 no.2
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• pp.173-183
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• 2014

A comprehensive mathematical model was developed for this study to estimate on-site and off-site GHG emissions from wastewater treatment plants (WWTPs). The model was applied to three different hybrid WWTPs (S-WWTP, J-WWTP, and T-WWTP) including anaerobic, anoxic, and aerobic process, located in Seoul City, South Korea. Overall on-site and off-site GHG emissions from S-WWTP, J-WWTP, and T-WWTP were $305,253kgCO_2e/d$, $282,682kgCO_2e/d$, and $117,942kgCO_2e/d$, respectively. WWTP treating higher amounts of wastewater produced more on-site and off-site GHG emissions. On average, the percentage contribution of on-site and off-site emissions was 3.03% and 96.97%. The highest amount of on-site GHG emissions was generated from anoxic process and the primary on-site GHG was nitrous oxide ($N_2O$). Off-site GHG emissions related to electricity consumption for unit operation was much higher than that related to production of chemicals for on-site usage. Recovery and reuse of biogas significantly reduced the total GHG emissions from WWTPs. The results obtained from this study can provide basic knowledge to understand the source and amount of GHG emissions from WWTPs and strategies to establish lower GHG emitting WWTPs.

• Korean Journal of Construction Engineering and Management
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• v.5 no.1 s.17
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• pp.114-123
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• 2004

Contract time determination for highway construction projects has never been easy despite considerable research efforts from academia as well as industry. High variations in crew production rates are considered one of the main barriers to accurate contract time determination. This paper presents a methodology for collecting field information on crew production rates which will help to enhance the accuracy of contract time determination for highway bridge construction. Based on a standard data collection tool developed, data on field crew production rates was collected from 14 on going projects in Texas, USA, over the past two years. The production rates based on the data collected were considered by industry practitioners to be more realistic and practical than those available to the current practices. As more data becomes available, key drivers influencing production rates could be identified and provide site personnel with a means to better plan and control production in a project specific context.

• 한국태양에너지학회:학술대회논문집
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• 2008.11a
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• pp.300-305
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• 2008

Using WindPRO that was software for windfarm design developed by EMD from Denmark, wind resources for the western Jeju island were analyzed, and the performance of WindPRO prediction was evaluated in detail. The Hansu site and the Yongdang site that were located in coastal region were selected, and wind data for one year at the two sites were analyzed using WindPRO. As a result, the relative error of the Prediction for annual energy Production and capacity factor was about ${\pm}20%$. For evaluating wind energy more accurately, it is necessary to obtain lots of wind data and real electric power production data from real windfarm.