• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1799-1808
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• 2006

A food-grade integration vector based on site-specific recombination was constructed. The 5.7-kb vector, pIMA20, contained an integrase gene and a phage attachment site originating from bacteriophage A2, with the ${\alpha}$-galactosidase gene from Lactobacillus plantarum KCTC 3104 as a selection marker. pIMA20 was also equipped with a controllable promoter of nisA ($P_{nisA}$) and a signal peptide-encoding sequence of usp45 ($SP_{usp45}$) for the production and secretion of foreign proteins. pIMA20 and its derivatives mediated site-specific integration into the attB-like site on the Lactococcus lactis NZ9800 chromosome. The vector-integrated recombinant lactococci were easily detected by the appearance of blue colonies on a medium containing $X-{\alpha}-gal$ and also by their ability to grow on a medium containing melibiose as the sole carbon source. Recombinant lactococci maintained these traits in the absence of selection pressure during 100 generations. The ${\alpha}-amylase$ gene from Bacillus licheniformis, lacking a signal peptide-encoding. sequence, was inserted downstream of $P_{nisA}\;and\;SP_{usp45}$ in pIMA20, and the plasmid was integrated into the L. lactis chromosome. ${\alpha}-Amylase$ was successfully produced and secreted by the recombinant L. lactis, controlled by the addition and concentration of nisin.

• Journal of Life Science
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• v.20 no.1
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• pp.97-102
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• 2010

Streptomyces platensis YK-2, newly isolated from forest soil, produces transglutaminase (TGase), which catalyses an acyl transfer reaction between the primary grade amine and protein or $\gamma$-carboxyamide group of peptide bound glutamine residues. For a molecular genetic study of S. platensis, an effective transformation method was established by using a conjugal transfer of DNA from Escherichia coli to spores of actinomycetes. The highest transconjugation frequency of S. platensis was obtained on an MS medium containing 50 mM $MgCl_2$, using $5{\times}10^7\;E$. coli as a DNA donor and $1{\times}10^8$ spores without heat treatment as a host. We also identified that S. platensis contains a single attB site within an ORF encoding a pirin-homolog, and that its attB site sequence shows high homology to that of S. logisporoflavus. In addition, it was confirmed by phenotypic analyses of exconjugants that the introduction of heterologous DNA into the attB site of the S. platensis chromosome does not affect its morphological differentiation and TGase production.

• Proceedings of KOSOMES biannual meeting
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• 2001.10a
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• pp.137-144
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• 2001

A Plankton ecosystem model was developed to investigate effects of hydrodynamic processes including advection and diffusion on size-structured phytoplankton dynamics in the mesohaline zone of the York River estuarine system, Virginia, USA. The model included 12 state variables representing the distribution of carbon and nutrients in the surface mixed layer. Groupings of autotrophs and heterotrophs were based on cell site and ecological hierarchy Forcing functions included incident radiation, temperature, wind stress, mean How and tide which includes advective transport and turbulent mixing. The ecosystem model was developed in FORTRAN using differential equations that were solved using the 4th order Runge-Kutta technique. The model showed that microphytoplankton blooms during winter-spring resulted from a combination of vertical advection and diffusion of phytoplankton cells rather than in-situ production in the lower York River estuary.

• Journal of architectural history
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• v.32 no.4
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• pp.51-62
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• 2023

Gyeongbokgungdo depicts the composition and layout of Gyeongbokgung Palace before the Japanese Invasion of Korea in 1592, as well as a monument related to pro-jamrye held at the site of Gyeongbokgung Palace in Yeongjo. Based on literature such as Dongguk Yeoji Seungram, such as Gyeongbokgungdo painted major buildings, government offices, and buildings in the backyard. In addition, the literature and the foot of the mountain, waterway, and Pond, which were identified as the site identified during the reconstruction process, are reflected, and some of the Gyeongbokgungdo contain reconstruction records. As such, Gyeongbokgungdo depicts Gyeongbokgung Palace in the early Joseon Dynasty and facilities built after the Imjin War based on the literature, and seems to have been produced around the time of reconstruction as it reflects the mountain and water system. In addition, the layout of the main hall of Gyeongbokgungdo was partially reflected in the reconstructed Gyeongbokgung Palace and used as a material to understand the layout of Gyeongbokgung Palace in the early Joseon Dynasty.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2020.06a
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• pp.142-143
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• 2020

A study was conducted on the production of LEFC using the Precast method, not the on-site construction. LEFC, Light Emotion Friendly Concrete, has the advantage of plastic rods being inserted to allow light to transmit, but because of the lack of adhesion to concrete, it leads to a decline in mechanical performance and durability. Therefore, it is necessary to apply precasting techniques to ensure homogeneous and superior quality of LEFC. In this study, wooden molds were used and plastic rods were arranged on porous acrylic plates. Prototyping was carried out with a UHPC mix proportioning to ensure flowability, self-consolidating performance and mechanical performance.

• International conference on construction engineering and project management
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• 2013.01a
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• pp.513-516
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• 2013

Earthmoving equipment's haul-route has a great influence on the productivity of the earth work operation. Haul-route grade is a critical factor in selecting the haul-route. The route that has low grade resistance contributes to increase machine travel speed and production. This study presents a mathematical model called "Hauling-Unit Optimal Routes Selecting system" (HUORS). The system identifies optimal path that maximize the earth-work productivity. It consists of 3 modules, i.e., (1) Module 1 which inputs site characteristic data and computes site location and elevation using GIS(Geographical Information System); (2) Module 2 which calculates haul time; (3) Module 3 which displays an optimum haul-route by considering the haul-route's gradient resistances (i.e., from the departure to the destination) and hauling time. This paper presents the system prototype in detail. A case study is presented to demonstrate the system and verifies the validity of the model.

• Journal of Soil and Groundwater Environment
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• v.20 no.3
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• pp.7-14
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• 2015

In this study, land farming and chemical oxidation of a diesel-contaminated site is compared to evaluate the environmental impact during soil remediation using the Spreadsheet for Environmental Footprint Analysis by U.S. EPA. Each remediation process is divided into four phases, consisting of soil excavation, backfill and transportation (Phase 0), construction of remediation facility (Phase 1), remediation operation (Phase 2), and restoration of site and waste disposal (Phase 3). Environmental footprints, such as material use, energy consumption, air emission, water use and waste generation, are analyzed to find the way to minimize the environmental impact. In material use and waste generation, land farming has more environmental effect than chemical oxidation due to the concrete and backfill material used to construct land farming facility in Phase 1. Also, in energy use, land farming use about six times more energy than chemical oxidation because of cement production and fuel use of heavy machinery, such as backhoe and truck. However, carbon dioxide, commonly considered as important factor of environmental impact due to global warming effect, is emitted more in chemical oxidation because of hydrogen peroxide production. Water use of chemical oxidation is also 2.1 times higher than land farming.

• Land and Housing Review
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• v.10 no.3
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• pp.67-75
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• 2019

The modular construction means that more than 70% of the parts such as walls, windows, electrical wiring, facility piping, bathrooms, and kitchen appliances are pre-assembled at the factory and transported to the site. It is possible to shorten the construction period than general construction work and to secure high quality through modular mass production since the modular construction works in the field at the same time as the modular production. However, there are only four domestic modular manufacturers, and each company's modular components and construction methods are different, so it is necessary to standardize them. Therefore, this study investigated the construction process centering on the stacking method of modular housing construction work applied to D site in Cheonan-si, and proposed the key points of quality management by construction stage. As the project was conducted as a pilot project for government R&D projects, some differences may occur from general modular housing construction. However, the construction process and quality control focus of each unit box type modular house analyzed in this study can be used as basic data in the future of modular housing construction. In addition, the results of this study can be used to establish construction standards, such as the development of checklists and establishment of standard processes.

• Industrial Engineering and Management Systems
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• v.9 no.3
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• pp.227-241
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• 2010

A memory module industry's supply chain usually consists of multiple manufacturing sites and multiple distribution centers. In order to fulfill the variety of demands from downstream customers, production planners need not only to decide the order allocation among multiple manufacturing sites but also to consider memory module industrial characteristics and supply chain constraints, such as multiple material substitution relationships, capacity, and transportation lead time, fluctuation of component purchasing prices and available supply quantities of critical materials (e.g., DRAM, chip), based on human experience. In this research, a directed graph-based supply network planning (DGSNP) model is developed for memory module industry. In addition to multi-site order allocation, the DGSNP model explicitly considers production planning for each manufacturing site, and purchasing planning from each supplier. First, the research formulates the supply network's structure and constraints in a directed-graph form. Then, a proposed genetic algorithm (GA) solves the matrix form which is transformed from the directed-graph model. Finally, the final matrix, with a calculated maximum profit, can be transformed back to a directed-graph based supply network plan as a reference for planners. The results of the illustrative experiments show that the DGSNP model, compared to current memory module industry practices, determines a convincing supply network planning solution, as measured by total profit.

• Journal of the Korea Organic Resources Recycling Association
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• v.21 no.1
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• pp.62-68
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• 2013

The effect of organic substance on its degradation rate in burial site was investigated using batch tests. Substrate were swine and cattle with the initial concentrations of 2, 4, 6, 8, and 10 g VS(volatile solids)/L, respectively. The highest methane production rates of swine and cattle were found at 2 g VS/L as 46.3 and 48.4 ml CH4/g VS.d, respectively. As substrate concentration increased, the methane production rate decreased. The inhibition constants were n and m that were estimated using nonlinear inhibition model. The values of n and m were inhibition constants of methane production rate and ultimate methane yield, respectively. The values of n and m were 4.9 and 0.6 on swine and 1.1 and 0.4 on cattle. The methane production rate was responded sensitively by increase and decrease of substrate concentration, whereas ultimate methane yield do not relatively. From a relation between n and m, inhibitory effect of substrate concentration was confirmed as uncompetitive inhibition.