• Clinical and Experimental Reproductive Medicine
• /
• 제39권3호
• /
• pp.107-113
• /
• 2012

Objective: To determine whether animal age impacts in vitro preantral follicle growth. Effects of hCG, stem cell factor (SCF), and/or insulin-like growth factor (IGF) supplementation in growth medium were also investigated. Methods: Intact preantral follicles were mechanically isolated from fresh ovaries of BDF1 mice and cultured in growth medium for 9 to 11 days. Surviving follicles with antrum formation were transferred to maturation medium for 14 to 18 hours. Follicle survival, antrum formation, and retrieval of metaphase II (MII) oocytes were compared among three age categories (4-5, 7-8, and 10-11 week-old). By using 7- to 8-week-old mice, preantral follicles were cultured in growth medium supplemented with hCG (0, 5, or 10 mIU/mL), SCF (50 ng/mL), IGF-1 (50 ng/mL), and SCF+IGF-1. Results: Seven- to eight-week-old mice showed a higher follicle survival and antrum formation and produced more MII oocytes compared to other groups. In the 7- to 8-week-old mice, supplementation of 5 mIU/mL hCG significantly enhanced the antrum formation but the percentage of MII oocytes was similar to that of the control. Supplementation of SCF+IGF-1 did not enhance follicle survival or antrum formation but the percentage of MII oocytes increased modestly (39.1%) than in the control (28.6%, p>0.05, statistically not significant). Conclusion: Seven- to eight-week-old mice showed better outcomes in growth of preantral follicles in vitro than 4- to 5- or 10- to 11-week-old mice. Supplementation of hCG enhanced antrum formation and supplementation of SCF+IGF-1 yielded more mature oocytes; hence, these should be considered in the growth of preantral follicles in vitro.

• 생물환경조절학회지
• /
• 제8권4호
• /
• pp.242-249
• /
• 1999

수출용 백침계오이 재배시 수출기간내에 다수확 할 수 있는 최적 육묘방법을 구명코자 1997년과 1998년 2년간 육묘일수와 관수량을 달리하여 실험한 결과, 육묘일수간에는 30일 육묘구가 가장 좋았고, 다음으로 40일, 20일 육묘구 순이었다. 총수량 및 상품수량도 30일 육묘구가 가장 많았고 40일, 20일 육묘구 순이었는데 20일 육묘구는 수확개시기가 늦어 초기수량이 많이 떨어졌고 40일 육묘구는 초기수량은 다소 많았으나 초세의 노화가 빨라 수량이 조기에 급감 되었다. 관수량의 다소는 묘소질에 영향을 미치지 못하였다

• 대한치과교정학회지
• /
• 제22권3호
• /
• pp.657-673
• /
• 1992

Orthodontic treatment of cleft patients is difficult as the growth is different from that of normal ones. So it is very important to know the characteristic features of the craniofacial morphology and growth pattern in unilateral cleft lip and palate patients. The materials for this study consisted of 55 normal males and 50 unilateral cleft lip and palate ones who received cheiloplasty and palatoplasty previously. The cleft subjects were divided into 4 groups according to their ages kto find out the growth pattern of hard and soft tissue, and to compare the features with those of normal ones. Each cephalogram analysed by McNamara method and others. The obtained results were as follows 1. In the unilateral cleft lip and palate subjects, forward growth of the maxilla was smaller than that of normal ones from 9 years old. So the maxilla was retruded. The maxillary incisors were severely retruded in all age groups. 2. The mandibular overall length and its anteroposterior position did not show any significant differences between two groups. But the height of ramus was very short and the mandible had vertical growth tendency to compensate for undergrowth of the maxilla in cleft subjects after 12 years of age. 3. Horizontal growth of the soft tissue in middle face was smaller than that of any other facial region from 9 years old. The vertical growth rate of upper lip was decreased as growing old. 4. In cleft subjects, the upper and lower facial component angle and the facial convexity angle were large. So their facial profile changed to straight or concave as growing old.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권5호
• /
• pp.629-634
• /
• 2012

This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follicles of 75 to $99{\mu}m$ in diameter were collected daily from 7- to 14-day-old neonatal mice, and subsequently cultured in ${\alpha}$-MEM medium. Number of primary follicles isolated, growth of the follicle during in vitro-culture and maturation of intrafollicular oocytes were monitored. Overall, mean number of preantral follicles per animal was improved from 10.7 to 88.7 as the age of follicle donors was increased from 7 to 14-day-old. Number of primary follicles was increased gradually up to 11-day-old (35.7 follicle per an animal), then reduced to 29 in 14-day-old (p = 0.0013). More follicles retrieved from 10-day-old or 11-day-old females maintained their morphological normality at the end of primary culture than the follicles retrieved from 9-day-old. Of those cultured, primary follicles retrieved from 11-day-old mice yielded largest larger number of early secondary follicles than the follicles retrieved from in the other ages (39 vs. 13 to 29%). More than 3.3-times increase (0.86 to 2.86; p<0.05) in an average number of mature oocytes per animal was observed in the group of 11-day-old, compared with 9-day-old. However, no difference was found in the percentage of primary follicles developing into the pseudoantral stage (21 to 30%; p = 0.5222) and in the percentage of oocytes mucified (32 to 39%; p = 0.5792). In conclusion, a positive correlation between retrieval time and follicle growth was detected, which influences the efficiency to derive mature oocytes by follicle culture.

• 한국수산과학회지
• /
• 제37권5호
• /
• pp.400-404
• /
• 2004

Growth performances of the selected and the non-selected red sea bream (Pagrus major) lines were evaluated up to marketable size. The offspring were mass-produced either from the selected line (the fourth generation of broods from mass selection) or the non-selected line (the second generation of wild broods). Early (59 to 103 days old, trial I) and late growth performances (4 to 24 months old, trial II) were evaluated based on communal rearing in land-based tanks and sea cages, respectively. In trial I, the selected line exhibited significantly higher values in both weight gain (WG) and specific growth rate (SGR) when compared to those observed in the non-selected line (P<0.05). In trial II comprising of three growth phases (4-9, 9-16 and 16-24 months old), selected line showed only slightly improved in WG and SGR at the beginning two growth phases (4-9 and 9-16 months old) (P>0.05), however, displayed significantly higher values at the last growth phase (16-24 months old) (P<0.05). Overall WG and SGR throughout the experiment (4-24 months) were also significantly higher in the selected line than the non-selected line (P<0.05). Consequently, mean body weight of the selected line at 24 months old was 1.4-fold (40.8{\%}\;increased) as compared to that of the non-selected line. This present result indicates that mass selection of this species against the growth traits might be quite effective, and the present advance has important Implication in the productivity enhancement of red sea bream aquaculture.

• The Korean Journal of Ecology
• /
• 제8권4호
• /
• pp.215-223
• /
• 1985

The fluctuation of old climate was studied by the analysis of radial growth in trees, since 1782. For the effects of environmental factors on the radial growth, the authors proposed the relative radial growth(Rg) concept modified the emperical equation of Fritts (1969) as follow: Yt=aebt Yt: theoretical annual ring width time t a and b: constants in different tree species Rg=Yt/Yt Rg: ratio of real radial growth to theoretical growth with age Yt: real annual radial growth Used the Rg value, the relationship between climatic conditions and the radial growth during 70 years was investigated, and the fluctuations in climate during the last 200 years with the historical records was detected.

• The Korean Journal of Ecology
• /
• 제14권3호
• /
• pp.327-343
• /
• 1991

Clonal growth and rhizome architecture of calmagrostis epigeios, were studied in a 15-year old coastal reclamed land. As c. epigeios patch grew from center to margin radially over time, concentric annuli were added. the radial increases of the mature patch were 80~130cm/yr. the patch ages of c. epigeios estimated by relationships between the diameter and is radial increament, were 1~6year old. There were the marked variations of density, phytomass, shoot height and inflorecence frequency with increasing age of rhizome system within the patch. the maximum performance occurred at 2-year-old concentric annuli of the patch. The growth phasic continuum with aging was divided into 5 grwth phases ; pioneer, building, matyre, senile and degenerate. The rhizome aging had a more significant effect on the inflorescence frequency than on the other attributes. new rhizomes tended to diverge from the direction of the parent ramet by sympodial growth. in a 1-year-old patch, average length of rhizome segment was 7cm, average number of internode was 6, and the tillering frequency was 27%. The tillering in the early stage resultd in the increase of shoot density and the producation of multibranching rhizome. as the result of that, circular patchwas formed in a year. The branching structure based on such facts was represented graphically in branching degree of 60 and 100. therefore, c. epigeios with clonal growth may establish and grow vigorously earlier than other glycophytes in high salt conditions such as the coastal reclaimed land.

• Ocean and Polar Research
• /
• 제39권4호
• /
• pp.279-292
• /
• 2017

We determined the maturity and growth of the Marbled sole, Pseudopleuronectes yokohamae based on monthly sampling in the West Sea of Korea from February 2009 to December 2010. Determination of sex of P. yokohamae was by gonadal inspection, and age and growth were determined by analyzing the otolith. The biological minimum size of P. yokohamae female was 24.5 cm. The seasonal changes in the ratio of a translucent zone to an opaque zone revealed that the end of the translucent zone of the otolith were annuli formed in May once a year. We compared 8 type growth equations with several length-at-age data. By using the length-at-monthly age data of these, the calculated von Bertalanffy growth equations were $L_t=41.7(1-e^{-0.47(t+0.24)})$ for females and $L_t=32.7(1-e^{-0.75(t+0.01)})$ for males. We estimated that 24.5 cm was the total length of 2 year old P. yokohamae spawns in the cold water season from January to April, and grows to 8 years old for females and 6 years old for males in the West Sea of Korea.

• 대한치과교정학회지
• /
• 제24권2호
• /
• pp.349-366
• /
• 1994

The method of treatment in skeletal Class III malocclusion must be chosen according to an etiology and timing of the treatment. Maxillry protraction has been used as an effective treatment method in growing children with maxillary deficiency. The efficacy of maxillary protraction has been viewed as a result of downward-backward displacement of mandible and compensatory dental displacement during the treatment rather than forward -downward growth of maxilla itself. In this study, 104 subjects treated with maxllary protraction, and 19 males and 21 females with known annual growth amount have been chosen longitudinally as treated group and normal group, respectively. And changes in position of maxilla, mandible and dentition have been comparatively analyzed on the lateral cephalometric radiographs by age. The results were as follows : 1. Treated group showed more forward movement of maxilla compare to the normal group and the mandible displaced backward compare to the normal group. 2. Downward movement of maxilla in treated group was similar to that of normal group with statistical signigicance in female 12 year old group and downward movement of mandible in treated group was similar to that of normal group. 3. In treated group, maxillary central incisor moved more forward than the normal group with statistical significance in male 8, 10 year-old groups and female 8, 9, 10 year-old groups. In treated group, downward movement of maxillary central incisor was similar to that of the normal group with statistical significance in male and female 7,8 year-old groups. Considering the above results and the duration of the treatment, the forward movement of maxilla due to maxillary protraction was effective compared to normal growth amount of the normal group.

• 한국산림과학회지
• /
• 제74권1호
• /
• pp.61-66
• /
• 1986

잣나무 풍매차대(風媒次代) 15개(個) 가계(家系)에 대하여 2개년간(個年間)의 수고(樹高) 및 근원직경생장(根元直徑生長)을 조사(調査)하여 단일본(單一木) 및 가계유전력(家系遺傳力)을 분산분석(分散分析)에 의(依)한 분산기대성분(分散期待成分)을 이용(利用)하여 추정(推定)한 결과(結果) 5년생(年生)때의 수고(樹高)에 대한 단일본(單一木) 및 가계유전력(家系遺傳力)은 각각(各各) $h^2_I=0.40$, $h^2_F=0.77$을 나타내었으며, 6년생(年生)에서는 $h^2_I=0.57$ 및 $h^2_F=0.89$로 추정(推定)되었다. 5년생(年生)과 6년생(年生)의 근원직경(根元直徑)에 대한 단일본(單一木) 및 가계유전력(家系遺傳力)은 $h^2_I=0.16$과 $h^2_F=0.53$ 및$h^2_I=0.26$, $h^2_F=072$를 나타내었다.