• Journal of Plant Biotechnology
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• v.34 no.2
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• pp.103-109
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• 2007

The increasing industrialization of the world has led to precipitous rise for the demand of petroleum-based fuels. The world is presently confronted with the twin crises of fossil fuel depletion and environmental pollution. The search for alternative fuels, which promise a harmonious correlation with sustainable development, energy conservation, efficiency and environmental preservation, has become highly pronounced in the present. Bioenergy is playing an increasingly important role as an alternative and renewable source of energy. Use of Bioenergy has several potential environmental advantages. The most important perhaps is reduction in life cycle greenhouse gases emissions relatives petroleum fuels, since bioenergy is derived from plants which convert Carbon dioxide ($CO_{2}$) into Carbohydrates in their growth. Bioenergy includes solid biomass, biomas and liquid bio-fuels which are fuels derived from crop plants, and include biomass that's directly burned. The two most important bio liquid fuels today are bioethanol from fermenting grain, grass, straw or wood, and biodiesel from plant seed oil.

• Journal of Plant Biotechnology
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• v.4 no.3
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• pp.95-101
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• 2002

Molecular faming has the potential to provide large amounts of recombinant protein for use in diagnostics and as therapeutics. Various strategies have been developed to enhance the expression level, stability, and native folding of recombinant proteins produced in plants. Few investigations into the subcellular distribution of recombinant proteins within plant cells have been published despite the potential to increase the expression level and impact the purification process. This review article discusses the current strategies used for targeting recombinant proteins to various subcellular locations and the advantages of targeting to seed oil bodies for molecular farming applications. Specifically, the affinity capture of antibodies using recombinant oilbodies is discussed.

• Journal of Life Science
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• v.29 no.2
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• pp.147-151
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• 2019

Soybean [Glycine max (L.) Merr.] is grown worldwide for its high protein and oil content. Anthocyanins from black soybean seed coats are known to have many pharmaceutical effects. Soybean cultivars with large seed sizes and black seed coats are needed by soybean farmers. However, antinutritional factors, like protein, stachyose, and Kunitz trypsin inhibitor (KTI) exist in raw mature soybeans. Genetic elimination or reduction of these components is needed in soybean breeding. The objective of this research was to develop new a soybean strain with black seed coats and green cotyledons that was KTI protein free and low in stachyose. Six parents were used. The presence or absence of KTI protein was detected using the Western blot technique. The content of stachyose in mature seeds was detected using HPLC. One new strain was selected from 11 $F_2$ plants with black seed coats and green cotyledons that lacked KTI protein. The new strain had black seed coats and green cotyledons and was KTI protein free and low in stachyose. The plant height of the new strain was 66 cm, and its 100-seed weight was 28.4 g. The stachyose content of the new strain was 2.59 g/kg. The new strain developed in this research will be used to develop new cultivars that are KTI protein free and low in stachyose.

• The Plant Pathology Journal
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• v.33 no.6
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• pp.543-553
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• 2017

Sesame (Sesamum indicum) is an important oil seed crop of Asia. Yields can be negatively impacted by various factors, including disease, particularly those caused by fungi which create problems in both production and storage. Foliar diseases of sesame such as Alternaria leaf blight may cause significant yield losses, with reductions in plant health and seed quality. The work reported here determined the incidence of Alternaria species infecting sesame seeds grown in the Punjab, Pakistan. A total of 428 Alternaria isolates were obtained from 105 seed samples and grouped into 36 distinct taxonomic groups based on growth pattern and morphological characters. Isolation frequency and relative density of surface sterilized and non-surface sterilized seeds showed that three isolates (A13, A47 and A215) were the most common morphological groups present. These isolates were further identified using sequencing of the Internal Transcribed Spacer (ITS) region of ribosomal DNA (rDNA) and the Alternaria major allergen gene (Alt a 1). Whilst ITS of rDNA did not resolve the isolates into Alternaria species, the Alt a 1 sequences exhibited > 99% homology with Alternaria alternata (KP123850.1) in GenBank accessions. The pathogenicity and virulence of these isolates of Alternaria alternata was confirmed in inoculations of sesame plants resulting in typical symptoms of leaf blight disease. This work confirms the identity of a major source of sesame leaf blight in Pakistan which will aid in formulating effective disease management strategies.

• Journal of Life Science
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• v.33 no.8
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• pp.651-662
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• 2023

Peroxisomes, known as microbodies, are a class of morphologically similar subcellular organelles commonly found in most eukaryotic cells. They are 0.2~1.8 ㎛ in diameter and are bound by a single membrane. The matrix is usually finely granular, but occasionally crystalline or fibrillary inclusions are observed. They characteristically contain hydrogen peroxide (H₂O₂) generating oxidases and contain the enzyme catalase, thus confining the metabolism of the poisonous H₂O₂ within these organelles. Therefore, the eukaryotic organelles are greatly dynamic both in morphology and metabolism. Plant peroxisomes, in particular, are associated with numerous metabolic processes, including β-oxidation, the glyoxylate cycle and photorespiration. Furthermore, plant peroxisomes are involved in development, along with responses to stresses such as the synthesis of important phytohormones of auxins, salicylic acid and jasmonic acids. In the past few decades substantial progress has been made in the study of peroxisome biogenesis in eukaryotic organisms, mainly in animals and yeasts. Advancement of sophisticated techniques in molecular biology and widening of the range of genomic applications have led to the identification of most peroxisomal genes and proteins (peroxins, PEXs). Furthermore, recent applications of proteome study have produced fundamental information on biogenesis in plant peroxisomes, together with improving our understanding of peroxisomal protein targeting, regulation, and degradation. Nonetheless, despite this progress in peroxisome development, much remains to be explained about how peroxisomes originate from the endoplasmic reticulum (ER), then assemble and divide. Peroxisomes perform dynamic roles in many phases of plant development, and in this review, we focus on the latest progress in furthering our understanding of plant peroxisome functions, biogenesis, and dynamics.

• Journal of the Korean Applied Science and Technology
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• v.40 no.1
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• pp.103-112
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• 2023

This study selected 9 species of domestic native plants (Baechohyang, Red Pine, Turmeric, Ginger, San parsley seed, Bulsugam, Hwangchil, Tangja, Valerian) that have been proven effective for depression, and based on the analysis results of each fragrance component, 9 species were selected. Antioxidant (DPPH, ABTS), cytotoxicity (MTS), and anti-inflammatory (Nitric oxide) experiments were performed by combining essential oils. As a result of the analysis of fragrance components, DL-Limonene (38.44%), g-Terpinene (8.9%), Estragole (5.18%), and a-Pinene (1.73%), which were previously studied in combination oil, were identified. DPPH radical scavenging ability, which is an antioxidant activity, showed 75.4%, ABTS radical scavenging ability was 74.04%, and NO production inhibition was 33.14% at a concentration of 5uL/ml with no cytotoxicity confirmed. Through this, the effect of domestic blending essential oils on the improvement or prevention of depression is verified, and scientific efficacy and ingredient studies are conducted in a mutually cooperative manner to seek solutions to depression and provide basic data to confirm whether or not depressive symptoms are improved. want to do.

• Research in Plant Disease
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• v.15 no.1
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• pp.23-29
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• 2009

Cooking oil and yolk mixture (COY), a environmentally acceptable plant protection agent, and COY+$CaCO_3$+neem oil mixture were studied to control the powdery mildew occurring on eggplant, paprika, cherry tomato and maturity tomato in glass houses and vinyl houses during 2005 to 2007. The morphological changes of the pathogenic fungi on the leaf surface before and after treatment of COY were observed. COY made of rape seed oil and COY+$CaCO_3$+neem oil mixture were sprayed three times with 5 days interval to foliar parts of eggplant, paprika and tomato and the disease development were examined 5 days after final spray. In eggplant, the control efficacy of COY to powdery mildew was 94.6%. In paprika, the control efficacy of COY to powdery mildew was 91.6% and that of COY+$CaCO_3$+neem oil mixture was 96.2% that revealed little higher than COY itself. In tomatoes(cherry or maturity tomato), the control efficacy of COY were about 91 %, however, when COY mixture were sprayed to tomato leaves and stems the powdery mildew was controlled completely. Typical and healthy mycelia, conidiophores and condia were observed through scanning electron microscope in COY unsprayed leaf surface, on the other hand destroyed and winkled mycelia and conidiophores were observed in COY treated leaves regardless host plants nor taxonomic differences of fungi.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.3
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• pp.179-186
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• 1989

According to the results from the experiments of selecting a suitable variety of forage rape and comparing productivity and nutrient quality between forage rape and oil seed rape, Velox appeared to be the most suitable variety in terms of productivity and nutrient quality at the southern area of Korea among the varieties used in the experiments. Consequently, Velox was grown under two different cultural methods, individual culture and population culture, and productivity and variation of nutrient quality during the growth period were observed and compared between the cultural methods. The results from the experiment are summarized as follows: 1. Although variations were great in plant fresh weight and plant dry matter weight among plants, individual culture showed significantly higher value in these characters at the 1 % level than population culture. Plant fresh weight and plant dry matter weight were largely dependent upon the amount of branches in case of individual culture. However, in population culture, they were equally dependent upon the amount of branches and main stem. There was no significant difference in dry matter percentage between cultural methods, and main stem showed highest dry matter percentage. 2. Content of crude protein was decreasing gradually as plants continued to grow. Individual culture showed higher content of crude protein than population culture from 90 to 120 days after sowing but vice versa from 180 days after sowing to flowering stage. Contents of fiber such as NDF, ADF, cellulose and lignin was low at the early stage of growth. It was increasing gradually as plants grew older and at the latter stage of growth plants under individual culture showed higher values in contents of fiber. 3. In vitro dry matter digestibility (IVDMD) of both stems and leaves was decreasing gradually as plants grew older. Plants under individual culture showed higher IVDMD of stems than plants under population culture, but no significant difference in IVDMD of leaves was observed between cultural methods.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.220-220
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• 2017

Soybean (Glycine max (L.) Merill) is a very outstanding material crop with high protein and oil contents. We conducted this study to evaluate forage value and usability of soybean varieties as livestock forage. Three soybeans cultivars, OT93-26, Geomjeongsaeol, and Pungwon, were evaluated for forage use in this study, and Kwangpyeongok and Yeongwoo were used as check forage corn and rice, respectively. The whole soybean plants were harvested at the R5 (beginning seed development)- and R6 (full seed)-reproductive stages for analyzing forage usability and quality. Days to harvesting of the R5 stage-OT93-26 was the shortest among the tested crops while that of Yeongwoo was 122 days. The fresh and dry matter yields of all 3 soybeans were greater at R6 stage than at R5. Crude protein of the soybean cultivars harvested had a higher compared to the rice and corn regardless of the harvesting stage. Contents of crude fiber, neutral detergent fiber and acid detergent fiber of Yeongwoo had the lowest whereas Pungwon harvested at R5 were the highest. Among the soybeans, digestible dry matter, dry matter intake, and relative feed value of R6-harvested Geomjeongsaeol and Pungwon were high more compared to those at the R5-harvested, but in case of OT93-26 those at R6 stage were inversely measured rather than those at R5 stage. In conclusion, soybean can be used as s forage with high nutritive value for livestock. Moreover, Geomjeongsaeol can be applied to develop new forage soybean varieties with high nutritive value, and R6 stage is the optimum harvesting stage in yield and quality of the tested soybeans more than R5.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.282-290
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• 2019

Cuphea viscosissima plants accumulate medium-chain fatty acids (MCFAs), i.e., those containing 8 ~ 14 carbons, in their seeds, in addition to the longer carbon chain fatty acids (≥16 carbons) found in a variety of plant species. Previous studies have reported the existence of three C. viscosissima MCFA-producing acyl-acyl carrier protein (ACP) thioesterases with different substrate specificities. In this study, CvFatB4, a novel cDNA clone encoding an acyl-ACP thioesterase (EC 3.1.2.14), was isolated from developing C. viscosissima seeds. Sequence alignment of the deduced amino acid sequence revealed that four catalytic residues for thioesterase activity are conserved and a putative N-terminal chloroplast transit peptide is present. Overexpression of CvFatB4 cDNA, which was under the control of the cauliflower mosaic virus 35S promoter, in Arabidopsis thaliana led to an increase in 16:0 fatty acid (palmitate) levels in the seed oil at the expense of 18:1 and other non-MCFAs.