• Title/Summary/Keyword: oil-crop

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Morphological characters, Total phenolic content, and Fatty Acid Compositions of Safflower (Carthamus tinctorius) Genetic Resources

  • Awraris Derbie Assefa;Young Jee Kim;Ae-Jin Hwang;Bich-Saem Kim;Jae-Eun Lee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.94-94
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    • 2020
  • Safflower, a draught and salt tolerant oil seed crop of Compositae family, has been cultivated around the world mainly as source of edible oils and dyes, where India, the USA, Mexico, Australia, and Ethiopia contributing about 85% of the production altogether. In this study we have characterized some selected morphological properties of safflower plant and determined the the total phenolic content (TPC) and fatty acid composition in seeds of 237 genetic resources. All the seed coats were white colored while the petals had red, yellow and white pigments. The yellow was the predominant petal color being recorded in 182 accessions followed by red occurring in 49 accessions. The petal color of 47 of the accessions changed with development while the 190 accession showed no change of color. The leaves are ovate to obovate, mostly with dentate (21 moderate and 205 weak) and few smooth (11) margins. The plant length, leaf length, and leaf width were ranged between 65.7 and 160.8 cm, 14.3 and 37.0 cm, and 3.3 and 12.1 cm, respectively. The TPC was determined using Folin-Ciocalteu method and fatty acid compositions were evaluated using gas chromatography. The TPC content ranged from 23.71 to 132.72 µgGAE/mg dried extract (DE). The seeds of safflower genetic resources accounted an average crude fat content of 26.25% (14.84 to 41.70%). The total fatty acid is mainly comprised of 71.72% linoleic acid (18:2) and 20.08% oleic acid (18:1) on average, the remaining palmitic acid (16:0), stearic acid (18:0) and linolenic acid (18:3) contributing 5.84, 2.23 and 0.15 %, respectively. The fatty acid composition of safflower seeds has shown great variability, where oleic and linoleic acid have a wide range of variation, from 9.23 to 83.35% and from 10.46 to 82.62%, respectively

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Analysis of Quantitative Trait Loci (QTLs) for Seed Size and Fatty Acid Composition Using Recombinant Inbred Lines in Soybean (콩 재조합자식계통을 이용한 콩 종자의 크기와 지방산 조성의 양적 형질 유전자좌 분석)

  • Kim, Hyeun-Kyeung;Kim, Yong-Chul;Kim, Sun-Tae;Son, Beung-Gu;Choi, Yong-Whan;Kang, Jum-Soon;Park, Young-Hoon;Cho, Young-Son;Choi, In-Soo
    • Journal of Life Science
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    • v.20 no.8
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    • pp.1186-1192
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    • 2010
  • Soybean [Glycine max(L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvements in economic traits, such as quality and oil constituents, arethe most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control seed size and fatty acid contents in soybean. 115 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Iksan10' were used. Narrow-sense heritability estimates based on a plot mean on 100 seed weight, saturated fatty acid (palmitic acid + stearic acid), and oleic, linoleic, and linolenic acid content were 0.72, 0.60, 0.83, 0.77 and 0.81, respectively. The 100 seeds weight was related to seven QTLs located on chromosomes 1, 3, 8, 9, 16 and 17. Two independent QTLs for saturated fatty acid content were identified on chromosomes 17 and 19. Five independent QTLs for oleic acid content wereidentified on chromosomes7, 11, 14, 16 and 19. Five QTLs for linoleic acid content were located on chromosomes 2, 11, 14, 16 and 19. Three QTLs for linolenic acid content were located on chromosomes 8, 10 and 19. Oleic, linoleic, and linolenic acid had one major common QTL on chromosome 19. Thus, linoleic and linolenic acid content were identified as common QTLs.

Study on the biological activity of Artemisia iwayomogi KITAMURA (한인진(韓茵蔯)의 생리활성에 관한 연구)

  • Song, Young-Eun;Ryu, Ji-Sung;Chung, Ju-Ri;Kwak, Joon-Soo;Kim, Dae-Hyang;Kim, Bum-Suk;Rim, Chai-Woong
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.2
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    • pp.116-123
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    • 2001
  • This study was carried out to investigate antioxidative, antimicrobial activity and the effect on hepatotoxicity in various extracts of Artemisia iwayomogi. The herb has been used widely for jaundice, hepatitis and liver cirrhosis in chinese medicine. Solid yield by various extraction solvents, 18.1%, was the highest in water extract. To find antioxidative activity in Artemisia iwayomogi was estimated radical scavenging effect by DPPH method in various extracts and change of the POV(peroxide value) of various extracts added in soybean oil during 20 days at $60^{\circ}C$. Radical scavenging effect by DPPH method was the most effective in methanol extract. Added 1,000ppm water extract and methanol extract in soybean oil, the POV of them, 46.8(meq/kg) and 50.8(meq/kg) were lower than that of control, 79.1(meq/kg), during 20 days storage. After antimicrobial activity of various extracts of Artemisia iwayomogi on bacteria was carried out by paper disc method, it found that the ethanol extract was the strongest activity on Vibrio parahaemolyticus. In vivo experiment was to investigate the effect of Artemisia iwayomogi water extract(AIWE) on hepatotoxicity by carbon tetrachloride$(CCl_4)$ in rats. The experiment groups were divided into five groups for recovery(for 3 days) and three groups for protection(for 10 days) in rat liver. The weights and morphological changes of liver and the body weight were examined in each groups. Compared with $CCl_4$ treatment groups$(CCl_4\;only)$, liver and liver/body(%) weights of AIWE pretreatment groups for 3 days and AIWE posttreatment groups for 10 days were declined. In macrography, fibrious exudates and swelling of liver were decreased in AIWE treatment groups. Accumulation of lipid droplets and necrosis of hepatocytes were also decreased in AIWE treatment groups in microscopically. In these results, AIWE seems to enhance hepato-protective and recoverable effect on $CCl_4$ induced hepatotoxicity in rats.

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Growth, Yield and Nutrient Uptake of Radish as Affected by Amount of Organic Fertilizer in a Volcanic Ash soil (화산회토에서 유기질비료 시용량이 무 생장과 수량 및 양분흡수에 미치는 영향)

  • Kim, Yu-Kyoung;Cho, Young-Yuen;Oh, Han-Jun;Kang, Ho-Jun;Yang, Sang-Ho;Moon, Bong-Chun;Jwa, Chang-Sook
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.829-846
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    • 2015
  • This study was conducted to determine the effects of organic fertilization rates on the nutrient accumulation and recovery in radish (Raphanus sativus L.) as well as growth and yield of radish in Jeju island. An understanding the relationships between organic fertilization rate, crop nutrient recovery and crop yield can assist in making organic fertilizer recommendation which balances crop value and environmental risk in organic cultivation. Nitrogen (T-N), phosphate ($P_2O_5$) and potassium ($K_2O$) were applied at 0, 115-35-40, 230-70-80 (standard application rate), 460-140-160, 230-200-100 (recommended application rate) and 158-53-35 kg/ha (customary application rate), respectively as the broadcast application of mixed organic fertilizer (N 4.5% - $P_2O_5$ 1.5% - $K_2O$ 1%) in combination with langbeinite ($K_2O$ 22%), 100% at sowing period. The organic fertilizer was made of organic materials like oil cakes. Total yield of radish, as fresh weight of roots, increased with increasing organic fertilizer doses to a maximum at rate of standard or soil-testing application. Nitrogen, phosphate and potassium accumulations of radish increased curvilinearly with increasing organic fertilization rate to a maximum at rate of N 460 - $P_2O_5$ 140 - $K_2O$ 160 kg/ha. However, nitrogen, phosphate and potassium use efficiency of applied organic fertilizer decreased curvilinearly or linearly with increasing organic fertilization rate. Application of organic fertilizer in combination with langbeinite (as a potassium source) had significant effect on the yield of radish. Organic fertilization on a basis of standard or soil-testing application rate is recommended for maximun radish yield in organic cultivation.

Studies on the Fruiting Phase of Rape Under the Different Cultural Conditions

  • Kae, B.M.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.77-87
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    • 1972
  • The fruiting phase of rape under transplanting and direct-sowing conditions has been studied at Mokpo during the 2 years period from 1970 to 1971. Two varieties, Yudal and Miyuki were used in this study. The planting space and sowing time were also incorporated into this study. The results could be summarized as follows: 1. The plant tape of rape was nearly umbrella-shaped of all, but has changed to the laid elliptical-shaped, broadly ovate and spindle-shaped under different varieties and cultural conditions in the plant diagram(Fig. 2). 2. The length of the primary branches for each nodes had a tendency to the symmetric apical curve with the apex at the upper 10-12th node in the transplanting. but to the upper bias apical curve with the apex at the upper 5-7th node in the dense-sowing(Fig.3). 3. The ear of main stem was longer, more pods, heavier 1, 000 grains and more grain yield than ear of primary branches of all, Especially, as for that, the rate of yield constitution per plant in the direct-sowing was higher than in the transplanting(Fig. 4, 5, 6, 7, 8, 9). 4. The ear-length of the primary branches for each nodes had a tendency to the relatively slowly apical curve with the apex at the upper 3-4th node in the transplanting, but to the lower bias apical curve with the apex at the upper 2nd node in the dense-sowing. Especially, the possibility of growth at the lower ears was few in the early variety (Fig. 4). 5. The number of pod per ear on the primary branches for each nodes had a .tendency to the curve of ear-length with the apex at the upper 5-8thnode in the transplanting and at the upper 4-5th node in the dense-sowing (Fig. 5). Accordingly, a high positive correlation was found between the ear-length and number of pod per ear (Table 2) 6. In the transplanting, the high rate of effective ear was from the upper nods to the 12th node, but below the 16-17th nodes was ineffective. However, in the early dense-sowing the high rate of effective was to the 7th node. but below the 10th nodes was. ineffective. Especially, in the early variety has difficult to secure of poi-numbers for ineffective of the lower nodes(Fig. 6.). 7. The density of pod setting of the ear of main stem was the longest of all ears, and the lower nods were, the shorter it became. That had a tendency to the evidently apical growth. However. in the early variety, it was lengthened according to growth of ear-length(Fig.7). 8. The pod-length of the medium nodes was longer than the upper and lower, and the possitive correlation between pod-length and number of grain per poi was very high(Table 2.). 9. In the grain yield per node of primary branches, the most yielding node of transplanting was the upper 9th node, of dense-sowing 4-5th node(Fig 8.), and the possitive correlation between grain yield per node and ear-length or number of pod per ear was very high(Table 2). 10. The grain yield of ear of main stem was higher than that of primary branches in the percentage of dependence for grain yield per plant. The limint node of 50% of dependence to cumulative grain yield per plant was the upper 7-8th node in tranplanting, in the early dense-sowing 4-5th node, and in tke late dense-sowing-3th node(Fig. 9). 11. In the weight of 1, 000 grains the lower nodes were, the lighter it becames in dense-sowing. Therefore, this was also lighter than in the transplanting to the (Fig. 10.). 12. The oil content of grain at the medium nodes was low in the early variety, but at the ear of main stem and upper 1st node it was extremely high(Fig. 11.).

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Genotype and Environment Influence on Raffinose and Stachyose Content of Soybean Seed (콩 종자의 Raffinose 및 Stachyose 함량에 대한 유전자형과 환경의 영향)

  • Sung, Mi Kyung;Han, Sung Jin;Seo, Hyung Jin;Choi, Sang Woo;Nam, Sang Hae;Chung, Jong Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.319-324
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    • 2014
  • Soybean (Glycine max (L.) Merr.) is an important crop for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. But, antinutritional factors in the raw mature soybean are exist. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. The objective of this research is to obtain the information on raffinose and stachyose content according to genotype and environment. A total of twenty two soybean genotypes (11 cultivars, 3 germplasms and 8 breeding lines) were selected. Each genotype was grown in the field for two years with two replications and harvested in bulk at natural maturity for two years. Content of raffinose and stachyose was detected by HPLC. The raffinose content (g/kg) of 22 genotypes was $2.68{\pm}0.21-5.87{\pm}2.43$ in year 1 and was $3.24{\pm}0.37-9.05{\pm}0.16$ in year 2. The stachyose content (g/kg) was $4.23{\pm}0.98-27.68{\pm}9.90$ at year 1 and was $5.11{\pm}1.09-25.32{\pm}0.35$ in year 2. Genotype and environment have highly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyose content at 5% significant level in two years. A positive correlation ($R^2=0.1985^*$) between raffinose and stachyose was observed in year 2. These informations are valuable in soybean genetics and breeding program related with raffinose and stachyose content.

Thermophilic Anaerobic Biodegradability of Agro-industrial Biomass (농축산바이오매스 고온 혐기성 생분해도 평가)

  • Heo, Namhyo;Kang, Ho;Lee, Seungheon
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.11a
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    • pp.101-101
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    • 2010
  • Anaerobic digestion(AD) is the most promising method for treating and recycling of different organic wastes, such as organic fraction of municipal solid waste, household wastes, animal manure, agro-industrial wastes, industrial organic wastes and sewage sludge. During AD, i.e. organic materials are decomposed by anaerobic forming bacteria and fina1ly converted to excellent fertilizer and biogas which is a mixture of carbon dioxide and methane. AD has been one of the leading technologies that can make a large contribution to produce renewable energy and to reduce $CO_2$ and other green-house gas(GHG) emission, it is becoming a key method for both waste treatment and recovery of a renewable fuel and other valuable co-products. Currently some 80% of the world's overall energy supply of about 400 EJ per year in derived from fossil fuels. Nevertheless roughly 10~15% of this demand is covered by biomass resources, making biomass by far the most important renewable energy source used to date. The representative biofuels produced from the biomass are bioethanol, biodiesel and biogas, and currently biogas plays a smaller than other biofuels but steadily growing role. Traditionally anaerobic digestion applied for different biowaste e.g. sewage sludge, manure, other organic wastes treatment and stabilization, biogas has become a well established energy resource. However, the biowaste are fairly limited in respect to the production and utilization as renewable source, but the plant biomass, the so called "energy crops" are used for more biogas production in EU countries and the investigation on the biomethane potential of different crops and plant materials have been carried out. In Korea, with steadily increasing oil prices and improved environmental regulations, since 2005 anaerobic digestion was again stimulated, especially on the biogasification of different biowastes and agro-industrial biomass including "energy crops". This study have been carried out to investigate anaerobic biodegradability by the biochemical methane potential(BMP) test of animal manures, different forage crops i.e. "energy crops", plant and industrial organic wastes in the condition of thermophilic temperature, The biodegradability of animal manure were 63.2% and 58.2% with $315m^3CH_4/tonVS$ of cattle slurry and $370m^3CH_4/tonVS$ of pig slurry in ultimate methane yields. Those of winter forage crops were the range 75% to 87% with ultimate methane yield of $378m^3CH_4/tonVS$ to $450m^3CH_4/tonVS$ and those of summer forage crops were the range 81% to 85% with ultimate methane yield of $392m^3CH_4/tonVS$ to $415m^3CH_4/tonVS$. The forge crops as "energy crops" could be used as good renewable energy source to increase methane production and to improve biodegradability in co-digestion with animal manure or only energy crop digestion.

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Development of multiplex PCR-based detection method for five approved LM canola events in Korea (Multiplex PCR 방법을 이용한 국내 승인 5개 LM 유채의 검출법 개발)

  • Jo, Beom-Ho;Lee, Jung Ro;Choi, Wonkyun;Moon, Jeong Chan;Shin, Su Young;Eum, Soon-Jae;Seol, Min-A;Kim, Il Ryong;Song, Hae-Ryong
    • Journal of Plant Biotechnology
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    • v.42 no.2
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    • pp.117-122
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    • 2015
  • Canola is a crop globally used for production of oil and biofuel. Cultivation area and import volume of living modified (LM) canola have been increasing every year. As canola import dependence has reached 100% in Korea, efforts have been made for safety management of LM canola and ecological risk assessment. We developed a set of multiplex PCR method for simultaneous detection of 5 LM canola events (Topas 19/2, Rf3, Ms8, RT73 and T45) approved in Korea. The multiplex PCR assay developed allows amplification of estimated products of 5 LM canolas from event specific primer sets. Primer extension time was skipped for a time-consuming process and two annealing steps (20 cycles at $55^{\circ}C$ and 20 cycles at $60^{\circ}C$) were performed for yielding the best result which was sufficient to distinguish five LM canolas. Our results suggest that multiplex PCR method provides a cost and time-effective approach for LM canola detection.

Integrated Eco-Engineering Design for Sustainable Management of Fecal Sludge and Domestic Wastewater

  • Koottatep, Thammarat;Polprasert, Chongrak;Laugesen, Carsten H.
    • Journal of Wetlands Research
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    • v.9 no.1
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    • pp.69-78
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    • 2007
  • Constructed wetlands and other aquatic systems have been successfully used for waste and wastewater treatment in either temperate or tropical regions. To treat waste or wastewater in a sustainable manner, the integrated eco-engineering designs are explained in this paper with 2 case studies: (i) a combination of vertical-flow constructed wetland (CW) with plant irrigation systemfor fecal sludge management and (ii) integrated CW units with landscaping at full-scale application for domestic wastewater treatment. The pilot-scale study of fecal sludge management employed 3 vertical-flow CW units, each with a dimension of $5{\times}5{\times}0.65m$ (width ${\times}$ length ${\times}$ media depth) and planted with cattails (Typha augustifolia). At the solid loading rate of 250 kg total solids (TS)/$m^2.yr$ and a 6-day percolate impoundment, the CW system could achieve chemical oxygen demand (COD), TS and total Kjeldahl nitrogen (TKN) removal efficiencies in the range of 80 - 96%. The accumulated sludge layers of about 80 - 90 cm was found at the CW bed surface after operating the CW units for 7 years, but no clogging problem has been observed. The CW percolate was applied to 16 irrigation Sunflower plant (Helianthus annuus) plots, each with a dimension of $4.5{\times}4.5m$ ($width{\times}length$). In the study, the CW percolate were fed to the treatment plots at the application rate of 7.5 mm/day but the percolate was mixed with tap water at different ratio of 20%, 80% and 100%. Based on a 1-year data of 3-crop plantation were experimented, the contents of Zn, Mn and Cu in soil of the experimental plots were found to increase with increasing in CW percolate ratios. The highest plant biomass yield and oil content of 1,000 kg/ha and 35%, respectively, were obtained from the plots fed with 20% or 50% of the CW percolate, whereas no accumulation of heavy metals in the plant tissues (i.e. leaves, stems and flowers) of the sunflower is found. In addition to the pilot-scale and field experiments, a case study of the integrated CW systems for wastewater treatment at Phi Phi Island (a Tsunami-hit area), Krabi province, Thailand is illustrated. The $5,200-m^2$ CW systems on Phi Phi Island are not only for treatment of $400m^3/day$ wastewater from hotels, households or other domestic activities, but also incorporating public consultation in the design processes, resulting in introducing the aesthetic landscaping as well as reusing of the treated effluent for irrigating green areas on the Island.

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Estimation on ability of livestock manure digestion for upland crops (밭작물별 가축분 소화능 계량화 평가)

  • Hyun, Byung-Keun;Yun, Hong-Bae;Kwon, Soon-Ik;Jung, Kwang-Yong;Koh, Mun-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.3
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    • pp.165-172
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    • 2001
  • Owing to raising number of livestock, we have a problem to solve disposal of livestock manure. We know that soil have the digestion ability of livestock manure as one of multifunctionality. I carried out to investigate of livestock manure digestion (especially pig and chicken manure) that is considered as nitrogen fertilizer in upland crops. The results were summarized as follows: 1. The amount of pig manure was(1999) 4,592,375 tons/year, and chicken manure was 4,488,166 tons/year and equivalent to 41,912 tons N/year and 76,223 tons N/year, respectively. 2. The definition of the digestion ability of livestock manure is as the maximum application amount of livestock manure without injuring soil and plant. And the calculation model of digestion ability of livestock manure(ALMD) is follows: ALMD = amount of nitrogen requirement per each upland crop / {(total nitrogen contents in livestock manure) ${\times}$ (nitrogen fertilizer efficiency of livestock manure)} 3. The amount of ability of pig and chicken manure for upland crops (dry based) were 1,142.9kg/10a and 540.1kg/10a, respectively. 4. The order of amount of digestion ability of livestock manure on upland were vegetables > orchards > miscellaneous grains(corn) > barley > potatoes > pulses > oil seeds & special crops ) fodder crops) mulberry.

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