• Korean Journal of Medicinal Crop Science
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• v.6 no.4
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• pp.299-304
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• 1998

This experiment was conducted to determine the essential oil content and the aromatic constituents in the leaves of Saururus chinensis Baill, Angelica dahurica Fischer and Cnidium officinale Makino. Volatile aromatic compounds in three aromatic medicinal plants were extracted with steam distillation extraction method and identified by GC/MS. Major aromatic compounds in Saururus chinensis Baill were 1,6-octadien-3-ol, 1, 3-benzodioxole, myristicin, ${\alpha}-cadinol$ and patchouene. Major aromatic compounds in Angelica dahurica Fischer were terpinolene, 3-carene, ${\beta}-caryophyllene$, ${\beta}-cubebene$, butylated hydroxy toluene, caryophyllene oxide, piperonal, and in Cnidium officinale Makino were aristolene, benzocycloheptene, ylangene, valencene, ${\beta}-cedrene$, satene, and menthofuran. Essential oil content was highest in Saururus chinensis plant.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.141-146
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• 1998

Production of medicinal soybean [Glycine max (L.) Merrill], characterized with black seed, white stripe at hilum border, yellow cotyledon and small seed, is increasing with increasing consumption. The objective of this study was to investigate the chemical composition of medicinal soybean seed and to provide basic information, for the characterization of these soybeans among genetic resources. Forty-four lines of medicinal soybeans collected from Korea and two control cultivars, 'Hwangkeumkong' (Yellow seed coat) and 'Geomjeongkong l' (Black seed coat) were planted at the Research Farm of the College of Natural Resources, Korea University, located at Namyangju City on May 25, 1996. Seeds of these lines were harvested at full maturity and analyzed for protein, oil, sugar, starch and mineral contents. Mean protein and oil content of the medicinal line seeds were 42.6 and 16.1%, respectively, and those of the control cultivars were in the middle range for protein and oil content. However, sugar and starch content of the medicinal line seeds appeared to be in the lower range of the distributions compared to the control cultivars and were 10.0 and 1.68%, respectively. Mean P, K, Ca, and Mg contents of the seeds of medicinal soybean lines were 15.9, 21.5, 3.11, and 2.81 mg/g, respectively, indicating that these lines had higher P, K, and Mg and lower Ca contents when compared to the control cultivars. Mean Na and Fe contents were 671 and 224 mg/kg, respectively, showing lower Na and similar Fe contents. The observed results provided that chemical compositions of medicinal soybean were, on average, different from those of the general soybean cultivars.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.298-302
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• 2006

A number of field experiments were conducted to assess the role of combined application of nitrogen and sulfur to increase the seed and oil yield of nonnodulating soybean (Glycine max (L) Merr.) cv. PK-416 $(V_1)$ and cv. PK-1024 $(V_2)$. Six combinations of N and S in three replicates each were used for this purpose i.e. $0\;S+23.5kg\;N\;ha^{-1}(T_1);\;0\;S+23.5+20kg\;N \;ha^{-1}(T_2);\;40\;S+23.5kg\;N\;ha^{-1}(T_3);\;40\;S+23.5+20kg\;N\;ha^{-1}(T_4);\;20+20\;S+23.5kg\;N\;ha^{-1}(T_5);\;20+20\;S+23.5+20kg\;N\;ha^l(T_6)$. Nitrate reductase (NR) and ATP-sulphurylase activities in the leaves were measured at various growth stages as the two enzymes catalyze the rate limiting steps of the assimilatory pathways of nitrate and sulphate, respectively. The activities of these enzymes were strongly correlated with seed yield. The higher seed, oil and protein yields were achieved with the treatment $T_6$ in both the cultivars due to optimization of NR activity and ATP-sulphurylase activity, as these parameters were influenced by N and S assimilation. Any variation from this combination was observed to decrease the activity of these enzymes resulting in reductions in the seed, oil and protein yield of soybean.

• Korean Journal of Medicinal Crop Science
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• v.21 no.6
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• pp.493-497
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• 2013

This study was designed to analyze the chemical composition of essential oil in 'Shiranuhi' immature fruit and to test their biological activities. 'Shiranuhi' immature essential oils (SIEO) were obtained by steam distillation from fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Fourteen components were identified in the essential oil. Limonene (75.21%) and terpineol (8.68%) were the major components in SIEO. Since acne vulgaris is the combined result of a bacterial infection and the inflammatory response to that infection, we examined whether SIEO possessed antibacterial against skin pathogens. As a result, SIEO showed excellent antibacterial activities against drug-susceptible and -resistant Propionibacterium acnes and Staphylococcus epidermidis, which are acne-causing bacteria. In this study, SIEO was examined on DPPH radical scavenging activities, which showed moderate antioxidant activity ($SC_{50}$, $15.36{\mu}L/mL$). In order to determined whether SIEO can be safely applied to human skin, the cytotoxicity effects of SIEO were determined by colorimetric MTT assays in normal human fibroblasts and keratinocyte HaCaT cells. They exhibited low cytotoxicity at $0.5{\mu}L/mL$ in both celllines. Based on these results, we suggest the possibility that essential oil of 'Shiranuhi' maybe considered as an antibacterial and antioxidant agent.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.41-46
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• 2015

In the present study, different expression of protein from Taekwang was revealed by 2-DE, and expressions of protein on each week after flowering was investigated. After analysis of expression of protein, MALDI-TOF was executed to identify expected protein function. Results revealed that there were three patterns of expression of protein during the maturing. The first pattern was that proteins were gradually expressed as up-regulation from 1 week to 6 week. The second pattern was that proteins were expressed gradually from 1 week to 5 week and then it started down-regulation in 6 week. The last pattern was that proteins were gradually as up-regulation from 1 week to 3 week and then down-regulation until 6 week. This phenomenon suggests that young stage has more protein related to correspondence mechanism against disease and growth and then maturing stage has more expression of protein related to storage protein. In MALDI-TOF analysis, p24 oleosin isoform A protein was identified that relates oleosin which is synthetic product in oil body. This protein spot increased gradually until 5 week and then decreased after 5 week. It explained that the protein is active until maturing stage to protect oil in seed and then its activity has gradually degraded. This result may be expected that a protein, related to growth of a seed has increased until maturing and then a seed fills up with a storage protein.

• Journal of Plant Biotechnology
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• v.37 no.3
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• pp.319-326
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• 2010

An interest in the production of seed-oil based fuel and raw materials, which comes from renewable plant sources, has been intrigued by the phenomenon of global warming and shortage of fossil fuels. Rapeseed (Brassica napus) is the most important oilseed crop, which produces seeds with 40% oil. It is desirable to develop genetically modified rapeseed producing oils, which can be easily converted to biodiesel. As an initial step for development of genetically modified rapeseed for the production of biofuels or bio-based materials, Korean rapeseed cultivars, Naehan, Youngsan, Tammi and Halla, were analyzed. Four Korean rapeseed cultivars produce 32 to 40% oil of seed dry weight, which is rich in oleic acid (more than 60 mole%). The cotyledonary petioles of rapeseed cultivar, Halla, were transformed using Agrobacterium tumefaciens strain GV3101, carrying the uidA gene encoding $\beta$-glucuronidase (GUS) as a reporter gene and the phosphinothricin acetyltransferase (PAT) gene as a selectable marker. The stable integration of PAT gene in the genome of transgenic rapeseeds was confirmed by PCR analysis. Expression of uidA gene in various rapeseed organs was determined by fluorometric assay and histochemical staining. Transformation efficiency of a Korean rapeseed Halla cultivar was 10.4%. Genetic inheritance of transgenes was confirmed in $T_2$ generation.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.172-176
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• 2011

'Suan' is a new $F_1$ hybrid variety of rapeseed (Brassica napus L.) with early maturing and high oleic acid. This hybrid variety was bred by the cross between Mokpo-CGMS (male sterile line) and 8516-B-5-6-5-3 (restore line) for the production of bio-diesel and edible oil in 2006. 'Suan' has green and parted leaf, yellowish flower, and black seed coat. 'Suan' is more tolerant to lodging and stem rot compared to 'Sunmang', check variety. The ripening date of 'Suan' is June 2nd which is 6 days earlier than 'Sunmang'. Yield trials were conducted from 2006 to 2007 and regional adaptation trials were examined at five locations each in 2008 and 2009. The average seed yield of regional adaptation trials was 381 kg/10a that was 4% higher than that of 'Sunmang'. Total oil content of 'Suan' was 44.3%. Oleic acid content was 68.3%, which is 5.2% higher than 'Sunmang' but, erucic acid was not detected. Total glucosinolate content was 2.31 mg/g. Therefore, this variety is recommended as a leading variety at southwestern area including Jeonnam, Jeonbuk and Kyongnam provinces of South Korea.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.664-668
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• 2009

"Boram" is a new $F_1$ hybrid of rapeseed (Brassica napus L.) with early maturing, high yield and high oleic acid. This cultivar was developed from the cross between Mokpo-CGMS (male steile line) and 8634-B-1-3-1-8 (restore line) for the production of edible oil and biodiesel in 2006. Yield trials were conducted from 2007 to 2008 and regional adaptation trials were examined at four locations in 2008. "Boram" has green and parted leaf, yellowish flower, and black seed coat. The ripening period of "Boram" is similar to "Sunmang", but 3 days faster than "Tammiyuchae". The average seed yield of the regional adaptation trials was 424 kg/10a, which was 11 and 48% higher than "Sunmang" and "Tammiyuchae" variety. Total oil content of "Boram" was 44.2%. In fatty acid composition, "Boram" has no erucic acid. However, oleic acid content is 68.5%, which is 3.7 and 5.4% higher than "Tammiyuchae" and "Sunmang". And total glucosinolate content was 2.27mg/g. Therefore this variety is recommended as a leading variety at southwestern areas including Jeonnam, Jeonbuk and Kyeongnam provinces and Jeju island of Korea.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.236-240
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• 2010

'Jomang' is a new $F_1$ hybrid variety of rapeseed (Brassica napus L.) with early maturation, high yield and high oleic acid. This hybrid variety was developed from the cross between Mokpo-CGMS (male sterile line) and 8516-B-5-6-5-3 (restore line) for the production of edible oil and biodiesel in 2006. Yield trials were conducted from 2007 to 2008, and regional adaptation trials were examined at four locations in 2008. 'Jomang' has green and parted leaf, yellowish flower and black seed coat. The ripening date of 'Jomang' is 1~4 days earlier than 'Sunmang' and 'Tammiyuchae'. The average seed yield of the regional adaptation trials was 406 kg/10a, which was 7% and 42% higher than those of 'Sunmang' and 'Tammiyuchae,' respectively. Total oil content of 'Jomang' was 44.5%. 'Jomang' has no erucic acid in fatty acid composition. However, oleic acid content is 68.2%, which is 3.4% and 5.1% higher than 'Tammiyuchae' and 'Sunmang,' respectively. Total glucosinolate content was 2.31 mg/g. Therefore, this variety is recommended as a leading variety at southwestern area including Jeonnam, Jeonbuk and Kyongnam provinces and Cheju island of Korea.

• Korean Journal of Environmental Agriculture
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• v.39 no.3
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• pp.263-272
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• 2020

BACKGROUND: This paper describes the successful discrimination of GM crops from the respective wild type (WT) controls using spectroscopy and chemometric analysis. Despite the many benefits that GM crops, their development has raised concerns, particularly about their potential negative effects on food production and the environment. From this point of view, the introduction of GM crops into the market requires the development of rapid and accurate identification technologies to ensure consumer safety. METHODS AND RESULTS: The development of a GM crop discrimination model using spectroscopy involved the pre-processing of the collected spectral information, the selection of a discriminant model, and the verification of errors. Examples of GM versus WT discrimination using spectroscopy are available for soybeans, tomatoes, corn, sugarcane, soybean oil, canola oil, rice, and wheat. Here, we found that not only discrimination but also cultivar grouping was possible. CONCLUSION: Since for the determination of GM crop there is no pre-defined pre-processing method or calibration model, it is extremely important to select the appropriate ones to increase the accuracy in a case-by-case basis.