• Title/Summary/Keyword: oil-crop

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Genetics and Breeding for Modified Fatty Acid Profile in Soybean Seed Oil

  • Lee, Jeong-Dong;Bilyeu, Kristin D.;Shannon, James Grover
    • Journal of Crop Science and Biotechnology
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    • v.10 no.4
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    • pp.201-210
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    • 2007
  • Soybean [Glycine max(L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates(palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid(16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups(LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content(18:1). Six QTLs($R^2$=4-25%) for increased 18:1 in N00-3350(50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid(18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2, and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3.

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Effect of Fertilization Rates on Growth, Root Yield and Essential Oil Composition in Korean Valerian (Valeriana fauriei var. dasycarpa Hara) (한국산 쥐오줌풀의 생육, 수량 및 정유성분 조성에 미치는 시비량의 영향)

  • Cho, Chang-Hwan;Han, Ouk-Kyu;Choi, Young-Hyun;Yun, Seung-Gil
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.814-820
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    • 1997
  • This study was conducted to establish standard fertilization rates for the cultivation of Korean valerian(Valeriana fauriei var. dasycarpa Hara) which is useful for medicinal and aromatic resources. Nitrogen, phosphate and potassium were treated with four application levels, 0, 6, 9, 12kg per 10a, and the growth characteristics, root yield and essential oil compositions of valerian were measured. The growth and root yield of valerian were the highest in the rate of 9kg per 10a for nitrogen, phosphate and potassium, respectively. Nitrogen had the most powerful effective followed by potassium and phosphate. The contents and compositions of essential oil also varied with the amount of fertilization. The contents of the essential oil was the highest in the application level of 9kg per 10a for nitrogen, phosphate and potassium, respectively. The essential oil compositions were affected by the fertilization rate.

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Variation of Seed Characteristic, Oil Content and Fatty Acid Composition in Sunflower Germplasm (해바라기 유전자원의 종실특성과 지방함량 및 지방산조성 변이)

  • Lee, Yun-Ho;Song, Hang-Lin;Piao, Xiang-Min;Park, Kyeong-Ho;Nam, Sang-Young;Kim, In-Jae;Choi, Sung-Yeol;Jang, Young-Seok;Kim, Hong-Sig
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.3
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    • pp.245-252
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    • 2010
  • The objective of this study was to obtain the basic informations on variability of germplasm and cultivation practice for the production of biodiesel in sunflower. A total of 275 accessions obtained from the National Agrobiodiversity Center (34 landraces from Korea, 219 and 22 introductions from America and other countries, respectively) were screened to evaluate variations of seed characteristics, oil content and fatty acid composition. Seed types were classified into 4 types (broad ovoid, narrow ovoid, rounded and elongated). The broad ovoid type was the highest proportion of 40.4%, while the elongated type was the lowest proportion of 5.0% among accessions. Also, the seed colors were classified into 4 colors (gray, brown, black and white). Gray and brown seed color showed the highest distribution and white seed color showed the lowest among accessions, respectively. Hundred and one liter seed weight ranged 2.9~15.5g and 178~439g with averages of 6.3g and 322.0g, respectively. Oil content ranged from 11.7% to 45.6% with an average of 25.5%. The frequency distribution of oil content showed the highest in range of 22~28% and the lowest in range of over 40%. Three promising accessions with higher oil content than 40.0% were IT031967, IT031970 and IT031965 introduced from America. Palmitic and stearic acid contents, saturated fatty acid, ranged 3.1~7.6% and 1.3~4.1% with averages of 4.7% and 2.2%, respectively, and a total content of saturated fatty acid ranged 5.4~9.4% with an average of 6.9%. Oleic and linoleic acid contents, unsaturated fatty acid, ranged 18.1~75.7% and 18.1~74.1% with averages of 55.2% and 38.0%, respectively. Five accessions, IT031831, IT031669, IT031895, IT031938 and IT031694, showed higher oleic acid content than 70%. A total content of unsaturated fatty acid ranged 89.6~94.8% with an average of 93.0%.

Fumigant Toxicity and the Repellent Effect of Essential Oils against Sitophilus zeamais Motschulsky (어리쌀바구미에 대한 식물정유의 훈증독성과 기피효과)

  • Kim, Yeon-Kook;Kim, Kwang-Ho;Lee, Jong-Jin;Lee, Hoi-Seon;Lee, Sang-Guei
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.389-395
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    • 2012
  • Stored grain insects appear frequently as grains are harvested, stored, and distributed on the market. Producers mostly depend on chemical fumigants to control and repel insect pests, but this application of chemicals may cause side effects for humans and the environment. Safer control approaches, including the use of natural products are required, and in this study, we assessed the control effect of essential oils on stored grain insects. The essential oils of 8 plant species from 6 families were tested for their fumigant toxicity and repellent effects using a Y-tube Olfactometer against Sitophilus zeamais adults. Pennyroyal and tea tree oil respectively exhibited 97% and 63% mortality at $10{\mu}{\ell}L^{-1}$ air concentration in tested fumigant toxicity. Of particular note, pennyroyal oil ($LT_{50}$, hour=3.87) showed higher adulticidal activities than tea tree oil ($LT_{50}$, hour=4.09). When assessing repellent effects, using a Y-tube Olfactometer at $10{\mu}{\ell}$/filter paper concentration, the cedarwood, cinnamon, cypress, patchouli and pennyroyal essential oils showed potent repellent activities against Sitophilus zeamais adults. Cinnamon, cypress and pennyroyal essential oils showed notably high repellent activities of 82.4%, 80.0% and 86.7%, respectively, at $1{\mu}{\ell}$/filter paper concentration.

Changes in Fatty Acid Composition and Phytosterol Content in Double Cropping Maize (이기작에 따른 옥수수의 지방산 조성 및 Phytosterol 함량 변화)

  • Kim, Sun-Lim;Jung, Gun-Ho;Kim, Mi-Jung;Son, Beom-Young;Kim, Jung-Tae;Bae, Hwan-Hee;Go, Young-Sam;Yi, Gibum;Lee, Jin-Seok;Baek, Seong-Bum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.2
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    • pp.93-103
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    • 2020
  • The average growth day of 11 maize varieties from planting to silking in the first cropping (FC) was 89.5 days and in the second cropping (SC) was 46.7 days, which was 43 days faster than in the FC. The average 100-kernel weight (100 KW) in the FC was 28.4 g and 18.3 g in the SC, which was approximately 36.4% lower than that in the FC. The average crude oil content of FC was 3.97% and SC was 3.08%, which was about 0.89% lower than that of FC. The composition of stearic and oleic acid was significantly higher in FC, whereas palmitic and linoleic acid were higher in SC; however, linolenic acid was not statistically different between the two crops. The crude oil content was negatively correlated with linoleic acid (FC -0.264ns, SC -0.504**) and positively correlated with linolenic acid (0.526**). Unsaturated fatty acid (USFA) composition showed a significant difference between FC (83.48%) and SC (82.96%). Total phytosterol content was 598.3 mg/100 g and 701.9 mg/100 g in FC and SC, respectively, and showed significant difference by planting dates. The β-sitosterol content showed no statistical difference between the planting dates, but campesterol and stigmasterol were significantly higher in SC than in FC. Therefore, it was considered that the temperature condition during the ripening period affects the 100 KW of maize, and this leads to the variation in phytosterol content. However, among phytosterols, β-sitosterol was relatively little affected by the planting dates. USFA composition showed a significantly negative correlation with phytosterol content. Considering the results, the relatively high phytosterol content in SC was mainly because of the delay in progress of starch accumulation as daily air temperature decreased from the middle of the ripening period, whereas progress of phytosterol accumulation in the maize kernel was considered relatively faster than that of starch accumulation.

Changes of Growth Characteristics, Rosmarinic Acid and Essential Oil Contents according to Harvest time in Agastache rugosa O. Kuntze (배초향의 수확시기별 생육특성 및 로즈마린산과 정유함량의 변화)

  • Lee, Sung-Woo;Kim, Jung-Bong;Kim, Kwan-Su;Kim, Myong-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.2
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    • pp.83-88
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    • 1999
  • To find out proper harvest time and to select a variety having high yield and good quality, growth characteristics and contents of rosmarinic acid and essential oil were investigated according to harvest time of Agastache rugosa O. Kuntze collected in Korea. Only the number of flower cluster per plant among growth characteristics of 7 collections showed not significant difference. The range of dry matter weight of above-ground part used as crude drug was $666{\sim} 1328kg/10a$ and the weight of flower cluster and root was not significant among the plant parts of collections. Dry matter weight of top was the highest on Sep. 20, full-bloom stage. Rosmarinic acid contents among in 7 collections showed significant difference and the range were $0.32{\sim}2.34%$ in flower, $0.97{\sim}1.64%$ in leaf, $0.03{\sim}0.21%$ in stem and $0.91{\sim}6.56%$ in root. The contents of rosmarinic acid and essential oil in leaf was 3.76%, 5.78% before flowering and 1.55%, 4.28% after flowering respectively. The total content of rosmarinic acid of all plant parts was decreased after flowering but that of essential oil was increased after flowering.

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Studies on the Heterosis Breeding in Rape by Using Cytoplasmic Genetic Male Sterile lines 3. Development of Complete Cytoplasmic Genetic Male Sterile Line "MOKPO-MS" Having Improved Quality of Oil and Oil Cake (세포질.유전자적 웅성불임 계통을 이용한 유채 Heterosis 육종에 관한 연구 제3보 유채 세포질 유전자적 웅성불임 계통의 불임안전성과 유.박의 성분 개량)

  • Lee, J.I.;Kwon, B.S.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.25 no.3
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    • pp.50-58
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    • 1980
  • A cytoplasmic-genetic male sterile line(MS) in rapes of which fertility is not restored under any temperature regimes and have the better quaility of and oil cake was developed. A MS line named Mokpo-MS, which is form the cross between Tower and Isuzu and its stamens is degenerated completely by interact~g with cytoplasmic and nuclear genes was selected. This MS line was found as a complete cytoplasmic-genetic male sterile having the quality of oil and oil cake were greatly improved by introducing Zero-erucic and Zero-glucosinolate gene from Tower.

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Changes of Seed Chemical Component during Grain Filling in Job′s Tears (율무의 등숙에 따른 종실 성분함량 변화)

  • Kim, Yul-Ho;Lee, Bong-Ho;Lee, Jung-Il;Hur, Han-Sun;Lee, Sang-Chul;Kim, Bong-Yoen
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.92-97
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    • 1994
  • The reports on the chemical composition of developing job's tears seeds is very insufficient by this time. The purpose of this study was to obtain the fundamental information on chemical component of the developing job's tears seed, including starch, sugar, oil, protein, fatty acid, Alkali Digestibility Value(ADV), 100-grain weight. Two job's tears varieties in different by maturity types were analyzed for those components from 10 days after heading to maturity, with 5-day intervals. Total sugar content in seeds followed a pattern of linear increase after heading, but it started to decreased around 15 days after heading. On the other hand, starch content continued to increased until maturity and the highest increasing occurred between 15 and 20 days after heading. The protein content of seeds was decreased, while oil content was increased through the ripening process. Starch content showed a positive correlation with 100-grain weight, ADV, oil and germination percent, but it had negative correlation between total sugar and protein contents in ripening process. Maximum starch content, 100 grain weight, and germination percent obtained by quadratic equation were attained 38 to 43 days after heading. So that the physiological maturity of job's tears seeds was considered to be 40 days after heading.

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Composition and Content of Soyasaponins and Their Interaction with Chemical Components in Different Seed-Size Soybeans

  • Kim Sun-Lim;Berhow Mark A.;Kim Jung-Tae;Chung Ill-Min;Chi Hee-Youn;Song Jin;Park Nam-Kyu;Son Jong-Rok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.4
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    • pp.340-347
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    • 2006
  • Soyasaponins $A_1$, DDMP-conjugated group B soyasaponins ${\alpha}g\;and\;{\beta}g$, non-DDMP counterpart soyasaponin I, II+III, and DDMP moiety were quantified in the large-, midium-, and small-seed soybean varieties. Protein contents were ranged from 38.1% to 41.8%, and oil contents were ranged from 15.5% to 18.9%, respectively. Oil contents in the large-seed varieties were significantly higher than those of medium- and small-seed varieties. Among detected soyasaponin peaks, ${\beta}g$ was a major soyasaponin in DDMP-conjugated group B soyasaponins followed by soyasaponin I, DDMP moiety and $A_1$. Soyasaponin concentration among different seed size soybean varieties. The soyasaponin concentration of mediumseed ($4014.5{\mu}g/g$) was slightly higher than those of largeseed ($3755.0{\mu}g/g$) and small-seed varieties ($3620.3{\mu}g/g$), however, the differences was statistically not significant. The composition rates of soyasaponins in the large-size seeds were 9.4% of soyasaponin $A_1$, 26.5% of DDMP-conjugated soyasaponins, 49.9% of non-DDMP counterpart soyasaponins, and 14.2% of DDMP moiety, respectively. Similar results were observed in the composition ratios of middle- and small-size seeds. Oil content and C:N ratio showed the significant positive correlations with total soyasaponin concentration, while the 100-seed weight, fiber, and ash contents showed the negative correlations with total soyasaponin but statistically not significant. It was noted that protein contents didn't have any relationship with group A, group B, DDMP moiety, and total soyasaponin. This fact suggested that protein contents are not affects the variation of soyasaponin concentration.

Schizandrin, Oil Compounds, and Their Extraction Yield in Fruits of Schizandra chinensis Baillon (오미자의 Schizandrin과 기름성분 및 추출수율 변화)

  • 김관수;박춘근;류수노;방진기;이봉호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.3
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    • pp.158-162
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    • 2000
  • Histochemical distribution and varietal variation of schizandrin and oil compounds, and the changes of their extraction yield were investigated in fruits of collected Schizandra chinensis including Inje cultivar. In histochemical analysis on the distribution of schizandrin and oil in fruits of Inje cultivar, higher concentrations of them were found in the seed (1.01% and 27.6%, respectively) than in the epicarp and mesocarp of the whole fruit. Average contents of schizandrin in fruits and oil in seeds of collected lines were 0.84% and 27.9%, respectively. The mean composition of fatty acids in seeds oil was 3.6% of palmitic acid, 0.6% of stearic acid, 19.7% of oleic acid, 73.0% of linoleic acid, and 3.1% of linolenic acid, showing high composition(95.8%) of total unsaturated fatty acid. Oil extracted from seeds of Inje cultivar contained 4.29% of schizandrin, indicating that seed oil contained much schizandrin, a bioactive lipid-soluble compound. Compared with 80% methanol extraction in fruits and seeds, yields of schizandrin and oil were lower showing 23.8% and 17.3%, respectively in boiling water extraction of the fruits and seeds without grinding. The seeds soaked with water during four months contained 1.18% of schizandrin and 25.2% of oil, whose contents were similar to those of the seeds stored at room temperature. These results demonstrated that the seed in the whole fruit could be utilized as a source to extract its functional oil and bioactive lipid-soluble compounds like schizandrin, especially after using Schizandra fruits for the beverage manufacture.

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