• The Korean Journal of Malacology
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• v.27 no.1
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• pp.77-86
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• 2011

The unwanted artificial oil-spill has severely contaminated the coastal environment in the world. Level of contamination has so far been monitered by various indicator species including mussel, oysters, flounder, and cockle. In this study, we decided to use the oyster as a model organism to observe the morphological changes being exposed to the artificial oil-spill in the coastal areas in Taean, Korea. The oysters were collected from four local sites (Sindu-ri, Uiwang-ri, Jonghyeon-dong, Ansan and Uihang-ri) exposed to various levels of pollution after an oil spill in Taean. Microscopic analysis of the hepatopancreatic microstructure in the digestive gland from the collected oysters show that the swelling, whorl, and destruction phenomenon of the nuclear membrane, a well-known microstructure induced by heavy metal exposure, was observed. Nuclear body (Nb), another typical characteristic of contamination or infection were also observed in some samples. Necrosis was observed in tissue samples collected from the area with a high degree of oil pollution. In addition, parasite-like particles (virus, perkinsus) were observed in most samples. Taken together, these results suggest that oil contamination in the oyster habitats influences the cytopathological changes in Crassostrea gigas.

• Journal of Forest and Environmental Science
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• v.29 no.3
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• pp.200-210
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• 2013

Biochemical characteristics of 24 Pongamia pinnata genotypes (candidate plus trees) from three agroclimatic zones were estimated and molecular characterization through RAPD markers was done. Various biochemical characters viz. seed oil, total carbohydrates, protein, acid value and Iodine number recorded significant variation among different genotypes. The highest seed oil content was 41.87% while seeds of 14 genotypes recorded above average (32.11%) for the trait. Seed oil and protein content exhibited a significant positive correlation and moderate heritability. Out of the initially selected twenty-five random primers, twenty-two RAPD primers were found to be highly reproducible and produced a total of 183 loci of which 147 (80.32%) loci were polymorphic. Percentage of polymorphism varied from 44% to 100% with an average of 80.62%. High level of genetic variation was found among different genotypes of P. pinnata. Both molecular and oil content (biochemical) markers appeared useful in analyzing the extent of genetic diversity in Pongamia and the result of these analyses will help to better understand the genetic diversity and relationship among populations. Overall, the Pongamia genotypes included in the study showed a correlation with their geographical origins such that genotypes from the same region tend to have higher genetic similarity as compared to those from different regions. However, in UPGMA based Nei's analysis, some genotypes were found not to be grouped based on geographical origins possibly due to the exchange of germplasm over time between farmers across the regions. The results from oil content analyses showed that several genotypes in 'Central and Western Plateau' agroclimatic zone of Jharkhand displayed a good potential for high oil content. The study provides insight about P. pinnata populations in Jharkhand (India) and constitutes a set of useful background information that can be used as a basis for future breeding strategy and improvement of the species.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2004.05a
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• pp.106-106
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• 2004

• Korean journal of applied entomology
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• v.39 no.4
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• pp.259-266
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• 2000

This study was carried out to investigate the status of spot damage by fruit piercing pests and the kinds of these pests on yuzu (Citrus junos) fruit in Koheung, the most chief producing district of yuzu fruit in Korea, from ’97 to ’99. The extent of spot damage by fruit piercing pests on yuzu was increasing in recent years. This damage of fruits was severe in the lower canopy than the high one from ground and intercropping groves between yuzu trees had a greater damage to compare with single cropping of yuzu. Spot damage of yuzu fruit was occurred mainly from late September to early November when yuzu fruit is enlarging and coloring yellow. The blackish concave spot on yuzu fruit surface was appeared in 3 days after introduction of Riptortus clavatus into a netted cage containing one yuzu fruit and the diameters of this spot was 8.3 mm. At 10 days after introduction, this spot changed into milky-white with 9.2 mm diameters. One concave spot has contained oil cells by 17.7 and its external appearances has unchanged until harvest. The kinds of piercing pests of yuzu fruit were investigated with 3 orders, 16 families and 37 species. These pests were classified by 11 species of bugs, 5 species of hoppers and 21 species of moths. Among them, dominant species were Physopelta gutta, Halyamorpha halys, Empoasca vitis, Aedia leucomelas, Agrotis tokionis, etc. Macroglossum bombylans and Acherontia s쇼x are newly confirmed species as the fruit piercing moths in Korea.

• Applied Microscopy
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• v.45 no.3
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• pp.144-149
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• 2015

Nothobranchius guentheri and Nothobranchius patrizii have special life cycle to sustain the dry season. So, we investigated the fertilized eggs morphology, and compared ultrastructures of surface structures and the cross section of fertilized egg envelopes using light and electron microscopes to determine whether these fertilized eggs and egg envelopes show the species specificity or have special structure to sustain the dry season. These fertilized eggs were spherical, yellowish, demersal and adhesive, and had a one-sided large oil droplet. The whip-like structures, adhesive filament were distributed throughout egg envelope in both species. But, that of N. guentheri was covered with fibrous structures, and that of N. patrizii was smooth. The egg envelope consisted of two distinct layers: an outer, electron-dense layer containing adhesive filaments and an inner layer of 16 to 17 horizontal electron-dense lamellae alternating with 15 to 16 interlamellae of lower electron density in both species. The external shapes of fertilized egg and section of fertilized egg envelope were same, but ultrastructure of adhesive filaments on the outer surface was concluded to show species specificity. Our data indicate that the ultrastructural differences of adhesive filament and outer surface of fertilized egg envelope show species specificity although these species belong to same genus.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.730-735
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• 2003

The effect of pathogenicity on trehalose accumulation was studied using six species of entomopathogenic nematodes adapted to either cold or warm temperature conditions. Also, trehalose accumulation was studied by treating nematodes with glycerol or oil at trehalose concentration and mortality of cold temperature $condition(5^{\circ}C)$, S. longicaudum Gl was increased 50% than control. And at trehalose concent and mortality of warm temperature $condition(30^{\circ}C)$, S. glaseri Agr B3 was increased 45%. Intracellular trehalose accumulation was increased with treatment with 20% glycerol or oil. As the final results, the pathogenic duration of nematodes was increased as intracellular trehalose accumulation was increased

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.805-809
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• 2004

The antimite activities of cumin seed oil-derived cuminaldehyde and eleven commercial components of Cuminum cyminum oil were examined against Dermatophagoides farinae and Dermatophagoides pteronyssinus adults and compared with those of benzyl benzoate and N,N-diethyl-m-toluamide. Responses varied according to dose and mite species. On the basis of $LD_{50}$ values, the compound most toxic to D. farinae adults was cuminaldehyde ($2.40\mug/cm^2$) followed by benzyl benzoate ($9.32\mug/cm^2$), thymol ($9.43\mug/cm^2$), DEET ($36.84\mug/cm^2$), and 3-carene ($42.11\mug/cm^2$). Against D. pteronyssinus adults, cuminaldehyde ($1.94\mug/cm^2$) was much more effective than benzyl benzoate ($6.50\mug/cm^2$) thymol ($6.92\mug/cm^2$), DEET ($17.79\mug/cm^2$), and 3-carene ($39.85\mug/cm^2$). These results indicate that the antimite activity of cumin seed oil could be caused by cuminaldehyde. Cuminaldehyde was about 3.9 and 3.4 times more toxic than benzyl benzoate against D. farinae and D. pteronyssinus adults, respectively. Therefore, further study is needed to confirm the findings of this study and the possibility of cuminaldehyde as a house dust mite control agent or a lead compound.

• Journal of Forest and Environmental Science
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• v.38 no.1
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• pp.12-20
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• 2022

Pongamia (Pongamia pinnata L.) as a source of non-edible oil, is potential tree species for biodiesel production. For several reasons, both technical and economical, the potential of P. pinnata is far from being realized. The exploitation of genetic diversity for crop improvement has been the major driving force for the exploration and ex situ/in situ conservation of plant genetic resources. However, P. pinnata improvement for high oil and seed production is not achieved because of unsystematic way of tree improvement. Performance of P. pinnata planted by Karnataka Forest Department was assessed based on yield potential by collecting 157 clones out of 264 clones established by Karnataka Forest Department research wing under different research circles/ranges. It was evident that the all the seed and pod traits were significantly different. Further, selection of superior germplasm based on oil and pod/seed parameters was achieved by application of Mahalanobis statistics and Tocher's technique. On the basis of D² values for all possible 253 pairs of populations the 157 genotypes were grouped into 28 clusters. The clustering pattern showed that geographical diversity is not necessarily related to genetic diversity. Cluster means indicated a wide range of variation for all the pod and seed traits. The best cluster having total oil content of more than 34.9% with 100 seed weight of above 125 g viz. Cluster I, II, III, IX, XV, XIX, XXI, XXIII, XXVI and XXVII were selected for clonal propagation.

• Journal of the Korean Society of Tobacco Science
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• v.27 no.1 s.53
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• pp.19-30
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• 2005

This study was to evaluate the biological activity of flavor components extracted from E. ciliata and E. splendens in order to survey the possibility applicable to tobacco and food industry. Flavor components were extracted with dividing into three parts; essential oil, absolute, oleoresin. In the nonenzymatic lipid peroxidation system, the inhibition rate($\%$) of essential oil were $67.3\;\pm\;20.7\%,\;58.1\;\pm\;19.3\%$ at the concentration of 50 ${\mu}g/mL$ of E. ciliata and E. splendens, respectively. The inhibition rate($\%$) of the oleoresin in E. ciliata was higher than one in E. splendens. In the enzymatic lipid peroxidation system, the inhibition rate($\%$) of essential oil and oleoresin was$14.28\;\pm\;2.38\%,\;and\;65.93\;\pm\;0.01\%,\;and\;was\;22.58\;\pm\;2.84\%\;and\;40.73\;pm\;6.04\%$. The oleoresin of two species were showed above $90\%$ of the inhibition rate($90\%$) against autooxidative lipid peroxidation system. $EC_{50}$ values in neutral red uptake assays 24 h of exposure times were $23.3\;{\mu}g/mL,\;341.0\;{\mu}g/mL\;and\;17.2\;{\mu}g/mL$ in essential oil, absolute and oleoresin from E. ciliata respectively, and were $46.4\;{\mu}g/mL,\;681.7\;{\mu}g/mL\;17.6\;{\mu}g/mL$ in three extractions of E. splendens. Oleoresin of two species showed high rate in the cytotoxic effect by neutral red uptake assay. Absolute and oleoresin did not show antibiotic and mutagenic activity. On the contrary, essential oil with over 500 ug/plate showed antibiotic and mutagenic activity in Ames test. Essential oil and oleoresin have a prolongating effect the ciliostasis of rat trachea. This results indicate that flavor components extracted from E. ciliata and E. splendens can be considered to be toxicological safe and to be the possibility applicable the cigarette, food and drug industry as a flavor for expectoration.

• Korean Journal of Environment and Ecology
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• v.31 no.6
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• pp.500-507
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• 2017

The study of insect fauna in Taeanhaean National Park in Korea began with the first survey of natural resources in 1996, and then the surveys were conducted seasonally from 2005 to 2014. The surveyed sites were mostly coastal areas, sand dunes, and back grasslands. Insects were collected by sweeping with insect net, suction, pitfall trap, light trap, and Malaise trap. As a result, a total of 1,540 species of 215 families belonging to 17 orders were identified. Lepidoptera was the most populous group at 34.2% and followed by Coleoptera at 28.3%, Hemiptera at 12.7%, Diptera at 8.5%, Hymenoptera at 7.1%, Orthoptera at 4.7%, Odonata at 2.0%, and others. The analysis of change of arenaceous insect fauna before and after the accident by the Hebei Spirit that spilt oil in Taean in December 2007 showed that 45 arenaceous insect species, mostly belonging to Coleoptera, were observed through the whole survey period. The impact of oil spill on the number of arenaceous insect species appearing in the area was minor.