• Title/Summary/Keyword: ocean science & technology

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Antioxidant Activity of a Chitin-degrading Bacterium Bacillus idriensis (CGH18) (키틴분해 박테리아 Bacillus idriensis (CGH18)의 항산화 활성)

  • Jung, Myoung Eun;Hong, Joo Wan;Lee, Jeong-Im;Kwak, Myoung Kuk;Kim, Hojun;Sohn, Jae Hak;Song, Young-Sun;Oh, Kwang-Suk;Seo, Youngwan
    • KSBB Journal
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    • v.28 no.4
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    • pp.217-224
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    • 2013
  • A bacterium CGH18 exhibiting antioxidizing and chitin-degrading activities in the colloidal chitin culture medium was isolated from salt-fermented crab. This strain was identified as Bacillus idriensis based on 16S rDNA sequence homology search. Its crude extract was partitioned between n-BuOH and $H_2O$. The organic layer was further partitioned between $CH_2$ $Cl_2$ and $H_2O$. Antioxidant activities of crude extract and its solvent fractions were evaluated using five different bioassay methods, including the degree of occurrence of intracellular reactive oxygen species (ROS), peroxynitrite scavenging (ONOO), and oxidative damage of genomic DNA. All fractions exhibited significant antioxidant activity in bioassay systems used.

Comparative Ecological Characteristics of Two Potentially Toxic Epiphytic Dinoflagellate Species, Ostreopsis sp. and Coolia canariensis, Native to Jeju Island

  • Mi Ryoung Oh;Hyung Seop Kim;Bora Jang;Jong Hyeok Kim;Keon Gang Jang;Jong Woo Park;Wonho Yih
    • Ocean and Polar Research
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    • v.45 no.2
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    • pp.57-69
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    • 2023
  • Growth responses along the gradient of water temperature, salinity, and light intensity and cytotoxicity against Artemia nauplii were explored using Ostreopsis sp. and Coolia canariensis strains, representing the two potentially toxic epiphytic dinoflagellate (EPD) species from Jeju coastal waters of Korea. Variation in maximum growth rate (GRmax) and maximum biomass yield (Ymax) along the environmental gradients was quite contrasting between the two strains, which appears to be reflected in the in situ abundance distribution of the corresponding genera. The more eurythermal characteristics of Ostreopsis sp. strain were in good agreement with the relative distribution of Ostreopsis spp. and Coolia spp. in 520 macroalgal samples collected from 6 stations. The more stenohaline C. canariensis strain was well matched by a markedly narrower range of salinities in the in situ distribution of Coolia spp. than the salinity range for Ostreopsis species. The differences in light adaptation between the high light-preferring Ostreopsis sp. strain and the more euryphotic C. canariensis strain were remarkably consistent with the distinct vertical profiles of Ostreopsis spp. and Coolia spp. abundance in the red alga Amphiroa sp. off Moom-seom. Cytotoxicity against Artemia nauplii in the Ostreopsis sp. preparation with 1000 cells ml-1 was similar to that in C. canariensis preparation with 12000 cells ml-1, which is noteworthy. Thus, the new potential cytotoxicity risks from C. canariensis along with the well-known toxic genus Ostreopsis may be introduced to Jeju coasts, which necessitates further exploration into the contrasting ecological niches occupied by EPD species in relation to their cytotoxicity.

Anesthetic and Physiological Effects of Clove oil and Lidocaine-HCl on the Grass Puffer, Takifugu niphobles

  • Gil, Hyun Woo;Lee, Tae Ho;Choi, Cheol Young;Kang, Shin Beom;Park, In-Seok
    • Ocean and Polar Research
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    • v.39 no.1
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    • pp.1-11
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    • 2017
  • The aim of this study was to determine the physiological response and the applicable concentration ranges of anesthetic clove oil and anesthetic lidocaine-HCl, and to investigate the synergistic effect of a mixture of these two anesthetics on the in grass puffer (Takifugu niphobles). The anesthesia times decreased and the recovery times increased with increasing concentrations of clove oil and lidocaine-HCl. Applicable concentration ranges for long-term transportation requiring more than 1 hour were 2 ppm for clove oil and 50 ppm for lidocaine-HCl. With mixtures of the two anesthetics, the anesthesia time decreased as the admixture concentration of clove oil and lidocaine-HCl increased. Anesthesia times of experimental groups with the combined anesthetics were shorter than those with the same concentrations of clove oil or lidocaine-HCl alone. Plasma cortisol concentrations were highest at 6 hours in all experimental groups anesthetized with the mixture of clove oil and lidocaine-HCl, while all groups with clove oil or lidocaine-HCl alone had the highest plasma cortisol concentrations at 12 hours. Plasma glucose concentrations were highest at 12 hours in experimental groups anesthetized with the mixture of clove oil and lidocaine-HCl, while groups with clove oil or lidocaine-HCl alone had the highest plasma glucose at 24 hours. The results of this study provide basic information about anesthetics and the synergistic effect of mixtures of anesthetics in this fish species. This information should be useful for aquaculturists who require methods for safe and easy fish handling, and for transporters who require that minimal stress is imposed on fish during transport.

First Record of the Giant Grouper Epinephelus lanceolatus (Perciformes: Serranidae: Epinephelinae) from Jeju Island, South Korea

  • Myoung, Jung-Goo;Kang, Chung-Bae;Yoo, Jae Myung;Lee, Eun Kyung;Kim, Sung;Jeong, Choong-Hoon;Kim, Byung-Il
    • Fisheries and Aquatic Sciences
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    • v.16 no.1
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    • pp.49-52
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    • 2013
  • A single specimen of Epinephelus lanceolatus, belonging to Epinephelinae of the family Serranidae, was newly collected from Jeju Island, South Korea, in December 2009. Epinephelus lanceolatus is characterized by anterior lateral-line scales with branched tubules, cycloid scales, irregular white spots, and fins with numerous small black spots. We propose a Korean name, "Dae-wang-ba-ri' for this species.

Impact of Environmental Variables on the Diversity and Distribution of the Megabenthos in the South Sea of Korea

  • Kang, Su Min;Yu, Ok Hwan;Lee, Hyung Gon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.3
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    • pp.354-365
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    • 2019
  • Megabenthos samples were collected using 10-min trawls towed at 17 stations from 2015 to 2016. The dominant species (>1% of the total density) were Stegophiura sterea (10.4%) and two subtropical species, Mactrinula dolabrata (9.0%) and Acila divaricate (8.3%), respectively. The community structure of the megabenthos fell into four groups: the southeast, the southernmost region off Jeju Island, the frontal zone of the South Sea with C3, and a diagonal area from the south coast to the western side of Jeju Island. The total numbers of species, diversity, density and biomass were higher in the C3 region of the South Sea. Environmental factor analysis showed that differences in the megabenthos community were related to depth, gravel contents, and sorting value (${\sigma}$). These results indicate that changes in the marine environmental conditions in the South Sea of Korea affect the megabenthos species' composition and diversity.

Weekly Variation of Phytoplankton Communities in the Inner Bay of Yeong-do, Busan (부산 영도 내만에서 식물플랑크톤 군집의 주간 변동 특성)

  • YANG, WONSEOK;CHOI, DONG HAN;WON, JONGSEOK;KIM, JIHOON;HYUN, MYUNG JIN;LEE, HAEUN;LEE, YEONJUNG;NOH, JAE HOON
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.26 no.4
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    • pp.356-368
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    • 2021
  • To understand the temporal variation of phytoplankton communities in a coastal area, the biomass and diversity were weekly investigated in the inner bay of Yeong-do, Busan. In the study area, chlorophyll a concentration ranged from 0.43~7.58 mg m-3 during the study, indicating the study area was in mesotrophic or eutrophic status. The fractions of chlorophyll a occupied by large phytoplankton (> 3 ㎛ diameter) exhibited an average of 80% of total chlorophyll a in this study. Among the large phytoplankton, while Bacillariophyta was the most dominant in spring and summer, Cryptophyceae prevailed in the fall and winter. On the contrary, in the picophytoplankton community less than 3 ㎛ in diameter, Mamiellophyceae was the most dominant in most seasons, Cryptophyceae was relatively high with an average of 17.7 ± 17.6% throughout the year, but seasonal variations were large. Dinophyceae rarely occupied a higher fraction up to 60.4% of the picophytoplankton community. By weekly monitoring at a coastal station for 13 months, it is suggested that phytoplankton communities in coastal waters could be changed on a short time scale. If data are steadily accumulated at the time-series monitoring site for a long time, these will provide important data for understanding the long-term dynamics of phytoplankton as well as the impact of climate and environmental changes.

Validation of the semi-analytical algorithm for estimating vertical underwater visibility using MODIS data in the waters around Korea

  • Kim, Sun-Hwa;Yang, Chan-Su;Ouchi, Kazuo
    • Korean Journal of Remote Sensing
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    • v.29 no.6
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    • pp.601-610
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    • 2013
  • As a standard water clarity variable, the vertical underwater visibility, called Secchi depth, is estimated with ocean color satellite data. In the present study, Moderate Resolvtion Imaging Spectradiometer (MODIS) data are used to measure the Secchi depth which is a useful indicator of ocean transparency for estimating the water quality and productivity. To estimate the Secchi depth $Z_v$, the empirical regression model is developed based on the satellite optical data and in-situ data. In the previous study, a semi-analytical algorithm for estimating $Z_v$ was developed and validated for Case 1 and 2 waters in both coastal and oceanic waters using extensive sets of satellite and in-situ data. The algorithm uses the vertical diffuse attenuation coefficient, $K_d$($m^{-1}$) and the beam attenuation coefficient, c($m^{-1}$) obtained from satellite ocean color data to estimate $Z_v$. In this study, the semi-analytical algorithm is validated using temporal MODIS data and in-situ data over the Yellow, Southern and East Seas including Case 1 and 2 waters. Using total 156 matching data, MODIS $Z_v$ data showed about 3.6m RMSE value and 1.7m bias value. The $Z_v$ values of the East Sea and Southern Sea showed higher RMSE than the Yellow Sea. Although the semi-analytical algorithm used the fixed coupling constant (= 6.0) transformed from Inherent Optical Properties (IOP) and Apparent Optical Properties (AOP) to Secchi depth, various coupling constants are needed for different sea types and water depth for the optimum estimation of $Z_v$.

The Effect of Antenna Pattern Measurement According to Radio Wave Environment on Data Quality of HF Ocean Radar (전파환경에 따른 안테나패턴 측정(APM) 결과가 고주파 해양레이더의 자료 품질에 미치는 영향)

  • Jae Yeob, Kim;Dawoon, Jung;Seok, Lee;Kyu-Min, Song
    • Ocean and Polar Research
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    • v.44 no.4
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    • pp.287-296
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    • 2022
  • High-frequency (HF) radar measures sea surface currents from the radio waves transmitted and received by antenna on land. Since the data quality of HF radar measurements sensitively depend on the radio wave environment around antenna, Antenna Pattern Measurements (APM) plays an important role in evaluating the accuracy of measured surface currents. In this study, APM was performed by selecting the times when the background noise level around antenna was high and low, and radial data were generated by applying the ideal pattern and measured pattern. The measured antenna pattern for each case was verified with the current velocity data collected by drifters. The radial velocity to which the ideal pattern was applied was not affected by the background noise level around antenna. However, the radial velocity obtained with APM in the period of high background noise was significantly lower in quality than the radial velocity in a low noise environment. It is recomended that APM be carried out in consideration of the radio wave environment around antenna, and that the applied result be compared and verified with the current velocity measurements by drifters. If it is difficult to re-measure APM, we suggest using radial velocity in generating total vector with the ideal pattern through comparative verification, rather than poorly measured patterns, for better data quality.

Temporal Variation of Phytoplankton Community Related to Water Column Structure in the Korea Strait

  • Lee, Yong-Woo;Park, Hyun-Je;Choy, Eun-Jung;Kim, Yun-Sook;Kang, Chang-Keun
    • Ocean and Polar Research
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    • v.32 no.3
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    • pp.321-329
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    • 2010
  • Photosynthetic pigments, nutrients, and hydrographic variables were examined in order to elucidate the spatio-temporal variation of water column structure and its effect on phytoplankton community structure in the western channel of the Korea Strait in fall 2006 and spring 2007. High phytoplankton biomass in the spring was associated with high salinity, implying that nutrients were not supplied by coastal waters or the Yangtze-River Diluted water (YRDW) with low salinity. Expansion of the Korea Strait Bottom Cold Water (KSBCW) and a cold eddy observed during the spring season might enhance the nutrient supply from the subsurface layer to the euphotic zone. Chemotaxonomic examination showed that diatoms accounted for 60-70% of total biomass, followed by dinoflagellates. Nutrient supply by physical phenomena such as the expansion of the KSBCW and the occurrence of a cold eddy appears to be the controlling factors of phytoplankton community composition in the Korea Strait. Further study is needed to elucidate the mechanisms by which the KSBCW is expanded, and its role in phytoplankton dynamics.