• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1135-1145
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• 2024

When cells are exposed to freezing temperatures, high concentrations of cryoprotective agents (CPA) prevent ice crystal formation, thus enhancing cell survival. However, high concentrations of CPAs can also cause cell toxicity. Exopolysaccharides (EPSs) from polar marine environments exhibit lower toxicity and display effects similar to traditional CPA. In this study, we sought to address these issues by i) selecting strains that produce EPS with novel cryoprotective activity, and ii) optimizing culture conditions for EPS production. Sixty-six bacteria producing mucous substances were isolated from the Ross Sea (Antarctic Ocean) using solid marine agar plates. Among them, Pseudoalteromonas sp. RosPo-2 was ultimately selected based on the rheological properties of the produced EPS (p-CY02). Cryoprotective activity experiments demonstrated that p-CY02 exhibited significantly cryoprotective activity at a concentration of 0.8% (w/v) on mammalian cells (HaCaT). This activity was further improved when combined with various concentrations of dimethyl sulfoxide (DMSO) compared to using DMSO alone. Moreover, the survival rate of HaCaT cells treated with 5% (v/v) DMSO and 0.8% (w/v) p-CY02 was measured at 87.9 ± 2.8% after freezing treatment. This suggests that p-CY02 may be developed as a more effective, less toxic, and novel non-permeating CPA. To enhance the production of EPS with cryoprotective activity, Response Surface Methodology (RSM) was implemented, resulting in a 1.64-fold increase in production of EPS with cryoprotective activity.

• Journal of Climate Change Research
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• v.34 no.16
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• pp.6841-6853
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• 2021

The leading modes of the North Pacific jet (NPJ) variability include intensity changes and meridional shifts in jet position on low-frequency time scales. These leading modes of NPJ variability are closely associated with weather and climate conditions spanning from Asia to the United States. In this study, we investigated changes in the NPJ's role as a conduit for U.S. surface air temperature (SAT) anomalies during the boreal winter across the late 1990s. We found that the leading mode of NPJ variability changed from the NPJ intensity changes to meridional shifts in NPJ position across the late 1990s. It leads to the change in the role of the NPJ as a conduit for U.S. SAT anomalies. Before the late 1990s, the variability of NPJ intensity significantly impacted western U.S. SAT anomalies related to the anomalous surface cyclonic circulation over the North Pacific. After the late 1990s, however, the variability of the NPJ's meridional shift significantly influenced the eastern U.S. SAT anomalies in association with the anomalous surface cyclonic circulation over the northern North Pacific. Further analysis and model experiments revealed that the western to central North Pacific Ocean has been warming since the late 1990s and modulates atmospheric baroclinicity. This phenomenon mainly leads to a northward NPJ shift and implies that the eddy-driven mechanism on the NPJ's formation, which acts to enhance the meridional variability of NPJ position, becomes dominant. We conclude that this northward shift of NPJ could have contributed to enhancing the NPJ's meridional shift variability, significantly influencing the eastern U.S. SAT anomalies since the late 1990s.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.1
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• pp.43-50
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• 2001

In order to analyze the dynamic motions of fish cage systems made of a frame and a netting under the conditions of waves and current, the hydraulic model experiment at towing tank and the numerical computation using boundary integral element method based on linear potential theory were carried out on a square and a circular type of fish cage, The computed and measured results for the dynamic motions of model fish cage systems showed that the heave and pitch motions were almost unaffected by the inclusion of nets, while the surge motions were very reduced by drag force acting on them. In addition, irregular wave-induced motions of fish cages included non-negligible 2nd order harmonic components at high frequency nearly twice the wave frequency. The reason why these motions were considered was due to resonance or structural components of frames being overflown and out of water during a wave cycle. It was found that circular type was more desirable structure in the open sea than square one only in the respect of dynamic motions due to waves and current. Further verifications were needed considering hydrodynamic forces, fatigue life, and structure analysis based on long term stochastic waves including frequency and time domain for the purpose of analyzing and designing fish cage systems.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.6
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• pp.549-558
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• 2012

A topology optimization method for phononic crystals is developed for the design of sound barriers, using the level set approach. Given a frequency and an incident wave to the phononic crystals, an optimal shape of periodic inclusions is found by minimizing the norm of transmittance. In a sound field including scattering bodies, an acoustic wave can be refracted on the obstacle boundaries, which enables to control acoustic performance by taking the shape of inclusions as the design variables. In this research, we consider a layered structure which is composed of inclusions arranged periodically in horizontal direction while finite inclusions are distributed in vertical direction. Due to the periodicity of inclusions, a unit cell can be considered to analyze the wave propagation together with proper boundary conditions which are imposed on the left and right edges of the unit cell using the Bloch theorem. The boundary conditions for the lower and the upper boundaries of unit cell are described by impedance matrices, which represent the transmission of waves between the layered structure and the semi-infinite external media. A level set method is employed to describe the topology and the shape of inclusions. In the level set method, the initial domain is kept fixed and its boundary is represented by an implicit moving boundary embedded in the level set function, which facilitates to handle complicated topological shape changes. Through several numerical examples, the applicability of the proposed method is demonstrated.

• Journal of Intelligence and Information Systems
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• v.7 no.2
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• pp.117-127
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• 2001

The Main object of this research is directed to LG Oil company harbor in kwangyang-hang, where various ships ranging from 300 ton to 48000ton DWT use seven berths in the harbor. This harbor suffered from inefficient and unsafe management procedures since it is difficult to set guidelines for arrival and departure of ships according to the weather conditions and the current guidelines dose not offer clear basis of its implications. Therefore, it has long been suggested that these guidelines should be improved. This paper proposes a decision-support system, which can quantitatively decide the possibility of entry or departure on a harbor by analyzing the weather conditions (wind, current, and wave) and taking account of factors such as harbor characteristics, ship characteristics, weight condition, and operator characteristics. This system has been verified using 5$_{th}$ and 7$_{th}$ berth in Kwangyang-hang harbor. Machine learning technique using genetic programming(GP) is introduced to the system to quantitatively decide and produce results about the possibility of entry or arrival, and weighted linear associative memory (WLAM) method is also used to reduce the amount of calculation the GP has to perform. Group of additive genetic programming trees (GAGPT) is also used to improve learning performance by making it easy to find global optimum.mum.

• Microbiology and Biotechnology Letters
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• v.45 no.2
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• pp.124-132
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• 2017

Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain Microbulbifer sp. (127CP7-12) was isolated from the Asian marine sponge Hymeniacidon sinapium collected at an intertidal zone on the west coast of Korea. Cultured bacteria produced a violet pigment, and optimal culture conditions for violet pigment production were investigated. Maximum production of the violet pigment from the strain culture was observed under the conditions of $25^{\circ}C$, pH 6.0, and 3% NaCl. Acetone provided better extraction of the pigment from fermented broth compared with ethanol and methanol. The proposed structure of the major component in the extracted crude pigment was determined via high-performance liquid chromatography, nuclear magnetic resonance, mass spectrometry, and UV spectra analyses, which showed that the metabolite was the promising bioactive compound violacein. This study describes the examination of marine bioactive materials from microbe-engaged metabolites and the ecological implications of the sponge-microbe association in a changing ocean.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.3
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• pp.247-252
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• 2002

To prepare the edible film based on fish protein, the optimal conditions for extracting soluble protein from Alaska pollack ( Theragra chalcogramma) and mackerel (Scomber japonious) muscle were defined. The effects of protein concentration, pH and temperature of protein solution on the physical properties of films were also investigated, Contents of moisture, crude protein, crude lipid and ash in Alaska pollack muscle were 79.6, 18.2, 0.6 and $1.2\%$, respectively. Contents of moisture, crude protein, crude lipid and ash in mackerel muscle were 69,1, 20.1, 9,5 and $1.3\%$, respectively. Both soluble protein contents extracted from Alaska pollack and mackerel were the highest at pH 12.0, and then un 2.0, 11.0. But they were extracted a little at neutral range. forward the recovery yield of protein by controlling isoelectric point was the highest at pH 4.8 ($79.8\%$) for Alaska pollack and at pH 5.0 ($64.1\%$) for mackerel, For the preparation of protein films from both Alaska pollack and mackerel, the most effective conditions of film forming solution were achieved, after supplied fish protein 4 g (glycerol 1,6 g) in 100 mL of distilled water, by adjusted to pH 10.0 and then heated at $90^{\circ}C$.

• Journal of the Korean Institute of Navigation
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• v.25 no.4
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• pp.347-360
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• 2001

The purpose of this study is to find out the subjective and objective fatigue level and the characteristics of seamen's physical strength with the service period on board, to have a new understanding of the vocation, and to explore a physical exercising program for the seamen. The questionnaire survey for the seamen who had been in service on the ocean going vessels was conducted and the results are fumed out as the followings. Total number of seamen who had answered the questionnaire was 64 persons and the distribution status of service period group was identified as 16 persons for each of 4 groups； the group was classified as the group of less than 5 years, 5 - 10 years, 10 - 15 years, and more than 15 years. 1. Working conditions Working hours in a week was identified as 44－56 hours(45.3%) and the number of 45.3% of the seamen answered the working hours in week is more than 56 hours. The seamen of 25.0% are unsatisfied or very unsatisfied with their vocation. The seamen of 65.6% are smoking, and 45.3% of them drink. The seamen of 44.,3% drink once or more in a week. And the seamen of 67.2% do not take any exercise in a week. 2. Contribution to the seamen's fatigue and subjective symptom The most contributive factor to the seamen's fatigue was identified as the short of sleeping(21.5%), overwork(19.2%), poor working conditions (18.6%), and the friction in human relations also becomes one the contribution to the fatigue(14.0%). The subjective symptom by fatigue was identified as the feeling of languid, sleepy and tiredness of eyes. 3. Characteristics of the seamen's physical strength and Interrelations The grip strength and back strength of the seamen whose service period is less than 5 years were measured as 40.44kg and 127.8kg. The sit ups in the group of service period less than 5 years was measured as 36.84 times, and the vertical jump for the seamen in this group was measured as 46.55cm. The closed-eyes foot balance was measured as 33.64 seconds in the group of service period between 5 and 10 years, and side-step was measured as 35.31 times in the group of service period less than 5 years. The standing trunk flexion was measured as 10.54cm for the seamen whose service period is between 10-15 years, and the trunk extension was measured as 50.70cm for the seamen whose service period is less than 5 years. And the step test of the seamen whose service period is between 10-15 years was measured as 54.44 numbers, and the number of flickers of the seamen whose service period is less than 5 years was measured as 32.94.

• Journal of the Korea Concrete Institute
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• v.25 no.1
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• pp.115-123
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• 2013

The steel industry, a representative industry that significantly consumes raw materials and energy, produces steel as well as a large amount of by-product steel slag through the production process. The vast habitat foundation of marine life has been destroyed due to recent reckless marine development and environment pollution, resulting in intensification of the decline of marine resources, and a solution to this issue is imperative. In order to propose a method to recycle large amounts of by-product slag into a material that can serve as an alternative to natural aggregate, the engineering properties and applicability for each mixing factor of environment friendly porous concrete as a material for the composition of marine ranches were evaluated in this study. The test results for percentage of voids per mixing ratio revealed that the margin of error for all conditions was within 2.5%. The compressive strength test results showed that the most outstanding environmental friendly porous concrete can be manufactured when mixing 30% slag aggregate and 10% specially treated granular fertilizer for the optimum volume fraction. As concrete for marine applications, the best seawater resistance was obtained with mixing conditions for high compression strength. An assessment of the ability to provide a marine life habitat foundation of environmentally friendly porous concrete showed that a greater percentage of voids facilitated implantation and inhabitation of marine life, and the mixing of specially treated granular fertilizer led to active initial implantation and activation of inhabitation. The evaluation of harmfulness to marine life depending on the mixture of slag aggregate and specially treated granular fertilizer revealed that the stability of fish is secured.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.28 no.10
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• pp.788-793
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• 2017

This paper presents the verification of accuracies of theoretical models for calculating the microwave reflections from rough sea surfaces. First of all, the Pierson-Moskowitz ocean spectrum was used to generate the rough sea surfaces. Then the relationship between the significant wave heights, root-mean-square(RMS) heights and wind speed was derived by estimating the significant wave heights and RMS heights of the generated sea surfaces according to various wind speeds, and compared the derived relationship with other measurement data sets. The reflection coefficients of the sea surfaces were calculated by using a numerical method(the moment method). Then, the numerical results were compared with Ament model, PO(Physical Optics) model, GO(Geometrical Optics) model and B-M(Brown-Miller) model for various roughness conditions(wind speed) and incidence angles. It was found that the Ament model is not accurate except for a very low roughness conditions($kh_{rms}$<0.4, k is wavenumber and $h_{rms}$ is RMS height). It was also found that at incidence angles lower than $70^{\circ}$, the PO and the GO models agree well with the numerical results, while the B-M model agrees well with the numerical analysis results at incidence angles higher than $80^{\circ}$ for very rough sea surfaces with $kh_{rms}$>10.