• BMB Reports
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• 제34권3호
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• pp.194-200
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• 2001

Secreted arginase from Evernia prunastri thallus has been purified 616-fold from the incubation medium. Purified arginase was resolved as only one peak in a capillary electrophoresis with a pI value of 5.35. The protein contained high amounts of acidic amino acids, such as Asx and Glx, and a relatively high quantity of Ser and Gly. The molecular mass of native, purified arginase was estimated as about 26 kDa by SE-HPLC. Substrate saturated kinetic showed a typical Michaelis-Menten relationship with a K_m value of 3.3 mM L-arginine. Atranorin behaved as a mixed activator of the enzyme (apparent $K_m$ = 0.96 mM); whereas evernic and usnic acid were revealed as non competitive inhibitors (apparent $K_m$ values were 3.16 mM and 3.05 mM, respectively). Kinetics of atranorin binding indicated that saturation was reached from 0.18 ${\mu}mol$ of the total atranorin and the occurrence of multiple sites for the ligand. This agrees with a possible aggregation of several enzyme subunits during the interaction process. A value of binding sites of about 12 was obtained. The binding of evernic acid was saturated from 23 nmol of total phenol. The number of binding sites was about 5. The loss of the binding ability of evernic acid could be interpreted as a single negative cooperatively. Usnic acid behaves in a similar way to evernic acid, although the binding saturation occurs at $0.14\;{\mu}moles$ of the ligand. This binding appears to be unspecific, and has 28 usnic acid binding sites to the protein.

• Journal of Welding and Joining
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• 제33권6호
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• pp.27-35
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• 2015

Laser welding sector in the automotive industry has been widely recognized as one of the most important bonding processes, such as parts welding. Efforts to improve productivity and weld quality have been progressing steadily. In addition, laser welding is suitable for welding process that can produce high-quality welds suitable for flexible production and small quantity batch productions. In order to ensure the rigidity of the material, high strength material are applied to more than 1 GPa class body parts and automotive bumper beams. However, not only the situation is that the trend of domestic research, but also development is based on product molding considering freedom of shape where reinforcement is applied to meet the safety regulations and high-speed crash performance, despite the use of high strength materials. The tendency for heat-affected zone (HAZ) softening phenomenon common in areas of laser welded high tensile steel welding confirmed the occurrence of weld softening effect according to the process parameters. Based on this, range of process parameters could be selected for ensuring weld quality.

• 한국물환경학회지
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• 제26권3호
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• pp.406-412
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• 2010

Concentrations and speciations of Trihalomethanes (THMs) and Haloacetic acids ($HAA_5$) that can be formed during chlorine disinfection by-product (DBPs) in full-scale drinking water treatment plants were investigated. Jeon-ju D water treatment plant that adopted conventional water treatment processes was chosen for investigation. SUVA values according to water treatment process changes were observed from 1.3 to 2.1. The process average concentrations of THMs was 7.4 ppb, 9.0 ppb and 14.7 ppb respectively, while the average concentrations of $HAA_5$ by each process which are precipitation water, filterater water, treated water, were 15.5 ppb, 14.9 ppb and 25.8 ppb respectively. DBPs concentrations was lower in the winter than summer. The major species of THMs was chloroform and the second highest was bromodichloromethane (BDCM) and the third highest was dibromochloromethane (DBCM). In case of $HAA_5$, the rate of trichloroacetic acid (TCAA) was detected. The species disribution of THMs is related to the change of SUVA and species disribution of $HAA_5$ is related to the concentrations of bromine and injection position of chlorine and injection quantity.

• The Korean Journal of Ecology
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• 제4권1_2호
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• pp.25-32
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• 1981

본 연구는 1975~77년에 이르기까지 한국의 석회암지대의 식피에서 나타나는 식물의 백화현성을 조사하였으며 석회질토양과 식물과의 관계를 연구하였다. 원주 I, II지역, 영월 I, II지역, 제천, 단양 및 삼척의 석회질 토양에서 발하고 있는 식피에서 60종의 식물이 백화현상을 나타내었으나 가지나 잎에서 부분적으로 일어나는것이 보통이였다. 농작물인 고추도 백화현상이 심하게 일어나고 있었다. 그러나 김의털을 비롯한 수종의 식물은 전조사지역에 걸쳐 전혀 백화현상이 나타나지 않았다. 석회질토양, 백화엽 및 정상엽에 함유되어 있는 중요무기양분의 분석결과를 비교연구한 결과, 석회질토양액의 알카리성 때문에 P와 Fe의 부용으로 야기되는 염석회식물들의 결핍증상으로 이들 석회암지대에서 나타나는 식물의 백화현상을 생각할 수 있다.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2008년도 추계 학술논문 발표대회
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• pp.37-41
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• 2008

According to the recent rapidly increasing that construction works are gradually Manhattanized mainly the grand scaled residential buildings, the foundation of the building that is related to safety is increasing for building as a grand scaled mat concrete. Because mat concrete can not be simultaneously placing of concrete in a great quantity due to the circumstance at the field, the inequal deformation of the tensile stress that according to the time lag of hydration heat between the upper layer and the lower layer is affecting as a cause that is the possibility of crack occurrence by increasing. Accordingly, this research checked the efficiency of super retard concrete in applying real structures, and we implemented the preparatory experiment to settle up the inequal deformation of the tensile stress substantially that is according to the time lag of placement between the upper layer and the lower layer by controlling the setting time using the super retarding agent. As the result of test, the more target of delay time lengthened, the more fluidity increased and air content indicated a little differences. There was from 2 to 10 hours between the standard curing and the outside curing at the setting time and in case of calculating the rate of mixing at real structure is required that mix promotion, increasing the amount of mixing, by setting up the curing temperature. The super retard concrete showed the result that in compressive strength, early-age strength was smaller than normal concrete whereas it was same or more figures from at the aging 28days because of the super retarding agent.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.247-247
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• 2017

Byobusan area of Aomori prefecture in Japan was a marshy sand dune and had developed for agricultural land use with a large-scale sprinkler system. Recently, it becomes an agricultural problem at this area that distinctive damage with browning maculation and fissures frequently occurs in Chinese yam tubers. Acetaldehyde is one of the factor candidates of underground part damage in plants. In this study, incidence rate of the tuber damage, and the morphological character and elemental composition of the damage parts in tubers were investigated with applications of excessive irrigation water or acetaldehyde water solution into the yam field. The incidence rate of the distinctive tuber damage increased as the input amount of irrigation water was increased. At the browning maculation parts of the tubers, many fissures and damages of cork layer were observed under scanning electron microscopy. In addition, the periderm of tubers was significantly thicker in damaged parts than in non-damaged parts. Funguses, bacterium and nematodes were not observed in the damaged part under scanning electron microscopy. The weight ratio of each constituent element in an analyzed area relative to the total weight of major essential elements was measured with energy dispersive X-ray spectrometry. The results showed that the weight ratios of boron, carbon, phosphorus, sulfur and calcium were higher in damaged parts than in non-damaged parts whereas the weight ratios of oxygen and chlorine were lower in damaged parts than in non-damaged parts. It was also shown by this spectrometry that iron, cadmium, lead and zinc were not directly involved in occurrence of the tuber damage. In this study, there was no remarkable difference of tuber appearance between non-acetaldehyde and acetaldehyde application treatments. From the above results, it is shown that the damage would be a physiological disorder induced by the input of a large quantity of water in the sandy field.

• Ocean and Polar Research
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• 제25권4호
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• pp.625-631
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• 2003

While phytoplankton diversity and productivity in the Southern Ocean has been widely studied in recent years, most attention has been given to elucidating environmental factors that affect the dynamics of micro-plankton (mainly diatoms) and nano-plankton (mainly Phaeocystis antarctica). Only limited effects have been given to studying the occurrence and the potential risks associated with the blooming of dinoflagellates in the relevant waters. This study focused on the appearance and toxicological characteristics of a toxic dinoflagellate, Alexandrium tamarense, identified and isolated from the Drake Passage in a research cruise from November to December 2001 The appearance of A. tamarense in the Southern Ocean indicates the risk of a paralytic shellfish poisoning (PSP) outbreak there and is therefore of scientific concern. Results showed that while the overall quantity of A. tamarense in water samples from 30meters below the sea surface often comprised less than 0.1% of the total population of phytoplankton, the highest concentration of A. tamarense (20 cells $L^{-1}$) was recorded in the portion of the Southern Ocean between the southern end of South America and the Falkland Islands. Waters near the Polar Front contained the second highest concentrations of 10-15 cells $L^{-1}$. A. tamarense was however rarely found in waters near the southern side of the Polar Front, indicating that cold sea temperatures near the Antarctic ice does not favor the growth of this dinoflagellate. One strain of A. tamarense from this cruise was isolated and cultured for further study in the laboratory. Experiments showed that this strain of A. tamarense has a high tolerance to temperature variations and could survive at temperatures ranging from $5-26^{\circ}C$. This shows the cosmopolitan nature off. tamarense. With regard to the algal toxins produced, this strain of A. tamarense produced mainly C-2 toxins but very little saxitoxin and gonyailtoxin. The toxicological property of this A. tamarense strain coincided with a massive death of penguins in the Falkland Islands in December 2002 to January 2003.

• 한국산림과학회지
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• 제97권3호
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• pp.332-339
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• 2008

경상북도 23개 시․군 산사태 발생지 172개소를 대상지로 선정하여 산사태 발생 특성을 다양한 요인별로 조사 분석하여 산사태 발생 위험 예측을 위한 판정기준표를 작성하였다. 산사태 위험 판정기준표는 수량화 I류를 이용하여 분석하였으며, 산사태 발생량에 영향을 많이 주는 요인은 경사위치, 경사길이, 모암, 방위, 임분경급, 종단명형, 경사도의 순으로 나타났다. 산사태 발생 위험 예측을 위한 산사태 붕괴 위험도 판정기준표를 작성한 결과, 107점 미만 : 안정(IV등급), 107~176점 : 위험도 소(III등급), 177~246점 : 위험도 중(II등급), 247점 이상 : 위험도 대(I등급)로 붕괴 위험도가 구분되었다.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2006년도 정기학술발표대회 논문집
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• pp.435-439
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• 2006

본 연구는 건설공사 불가변동으로 인한 계약금액조정을 함에 있어 산출과정에 있어서의 문제점과 물가변동 조정 후 설계변경이 수반될 때 물가상승액이 변경되면서 일어나는 비합리적인 정산방식과 법적인 문제점에 대한 개선방안을 제시하고자 수행되었다. 이를 위해 계약관리자를 대상으로 한 설문조사와 건설공사사례를 분석하였고 그 결과로 물가변동제도의 대한 문제점을 분석하였다. 위와 같은 과정을 통해 계약금액조정에 판한 개선방안 및 산식을 제안하였다. 상기와 같은 목적과 방법에 따라 수행된 본 연구의 결과는 다음과 같다. 1) 계약금액조정에 대한 개선방안으로는 합계단가로 비목군편성, 물가변동 조정 후 설계변경 수반시 증가된 물량 또는 신규품목에 대하여 설계변경당시 시점으로 물가변동 산출 등을 제시하였다. 2) 물가변동으로 인한 계약금액조정 후 설계변경이 수반될 경우 발생하는 기존물량 삭제 또는 물가변동제외금액 이하로 수량이 변경되는 경우와 수량 변경이 없는 경우에 대한 물가상승액 정산산식을 제안하였다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.539-546
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• 1996

The physical condition of rapeseed delivered to fat industry plants plays a significant role in the formation of the qualitative features of the raw material for oil production and, consequently , of the oil itself. Rapeseed is stored in silos , frequently for months, before it is subjected to processing. During the long storage, the conditions of the seed cover is very important, as the seed cover provides natural protection of the seed against the effect of the environment. Seeds with damaged seed cover are more easily affected by mildew, and the rate of chemical processes. Deteriorating the quality of oil contained in the cotyledons is faster in such seeds. Cracked seed covers facilitates also the growth and development of microorgaism. So as rapeseed damage occurring inthe course of harvest and the post-harvest processing have a negative effect of the quality and quantity of oil the sees contain. The study presented here was aimed at examining the typical process of purcha ing and handling of rapeseed in fat industry plants, in the aspect of the occurrence of mechanical damage to the seeds. Special attention was paid to the condition of rapeseed immediately after combine harvesting : next , the successive stages of technological handling of the seed were examined. observing the operation parameters of the particular machinery and equipment in order to identify those operations which caused deterioration in the quality of the material (sees). Seed samples were taken successively from the following the hopper, prior to cleaning , after cleaning , prior to drying , after drying, from dry rape silo. The total level of damage increased through the handling. The content of unusable contaminants had the tendency to decrease in the successive operations. though the actual values still exceeded levels permitted by standards. The study allow to indicate the operation fo postharvest technological process, which cause the most seed damage as well as gave quantita ive description of the losses occurred.