• 한국식품영양과학회지
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• 제39권7호
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• pp.1078-1082
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• 2010

본 연구는 해조류 세포벽에 결합된 무기질은 모두 이용되지 않는다는 전제하에 해조류의 세포벽물질(중성세제불용성섬유: neutral detergent fiber, NDF)을 측정하고 이에 결합된 미량 무기질 Fe, Cu, Zn, Mn의 함량을 조사하여 해조류내에 함유되어 있는 무기질 함량에서 빼줌으로써 계산하여 해조류의 무기질 이용률을 추정하였다. 해조류 총 7종에 대해, 해조류 100 g당 NDF에 결합된 무기질 Zn, Cu, Mn의 평균 함량은 각각 1.78, 0.43, 0.23 mg으로 그에 따른 이용률이 각각 90.56, 85.43, 87.26%로 모두 85% 이상의 높은 이용률을 나타냈다. 반면, Fe은 NDF에 결합된 무기질 함량이 22.42 mg/100 g으로 비교적 많은 양이 결합되어 있어 그 이용률은 68.36%로 낮은 이용률을 보였다. Fe이 NDF에 가장 많이 결합한다는 보고와 같이 해조류에서도 식이섬유인 NDF에 결합된 Fe의 함량이 많아 그 이용률은 상대적으로 낮았다. 하지만 그 외 Zn, Cu, Mn의 이용률은 85% 이상으로 높아 인체 내의 소화율을 고려한다 하더라도 이들 무기질은 높은 추정 이용률을 보일 것으로 판단되어 인체 영양상 미량 무기질의 좋은 식품자원으로써 그 이용을 기대해 본다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1303-1308
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• 2003

Samples of eleven brown midrib (ICSU 96031, ICSU 93046, ICSU 96082, ICSU 96078, ICSU 96075, ICSU 95101, ICSU 96034, ICSU 96063, ICSU 45116, ICSA 93-3 and ICSA 3845 X 3816) and nine white midrib genotypes (ICSU 96050, ICSU 96030, ISU 95082, SSG 59-3, FSHI 93-1, FSHI 2219A X 3211, HC 171, ICSA 93-2 and ICSA 93-1) based on their phenotypic appearance were collected at 50 per cent flowering from the sorghum germplasm grown at Research farm of IGFRI, Jhansi. These genotypes were evaluated with respect to crude protein, fiber composition, in-sacco dry matter, OM, cell wall components disappearance/digestibility besides the fodder yield, total phenolic and availability index values. Brown midrib genotypes were lower (p<0.05) in NDF, ADF, cellulose and acid detergent lignin concentration than white midrib genotypes. Mean NDF, ADF, cellulose and lignin contents were 69.4, 42.1, 35.4 and 5.7% in brown mid rib vis-a vis 75.8, 47.5, 39.6 and 7.3% in white mid rib genotypes. Nonsignificant (p>0.05) differences were observed in dry matter, crude protein and organic matter contents between brown midrib and white midrib genotypes. Phenolic contents were significantly (p<0.05) lower in browm mid rib (0.2) than white mid rib (0.3%) sorghum. Brown midrib genotypes exhibited significantly (p<0.05) higher in-sacco DM, OM and CP disappearance than normal (white midrib) genotypes. The mean degradability of DM, OM and CP was 64.1, 62.6 and 79.6% in brown mid rib and 53.1, 54.0 and 76.6% in white mid rib genotypes, respectively. There were no significant (p>0.05) differences between genotypes in extent of fiber fraction degradability though in-sacco NDF and ADF degradability was more by 5 and 4 units, respectively in brown midrib genotypes vis-a-vis white midrib genotypes. Average fodder yield (green and dry g/plant) and availability index (%) values were significantly (p<0.05) higher for brown midrib (474.2, 129.8 and 80.4) genotypes than white midrib (375.0, 104.8 and 69.2) genotypes. Lignin contents had significant negative correlation with DM, OM, NDF and ADF degradability. The results of the study revealed that brown midrib genotypes are superior not only with regard to chemical entities and disappearance of DM and fiber fractions but also better in respect of fodder yield and availability index values. Thus, brown midrib sorghum strains may be useful in increasing digestibility, intake, feed efficiency and animal performance.

• Asian-Australasian Journal of Animal Sciences
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• 제15권2호
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• pp.222-237
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• 2002

Effects of high and low levels of feeding with or without protected protein on the performance of lactating goats were studied. Twenty four German Fawn goats either from 1st ($43.37{\pm}3.937$ kg and 2 year old) or 3rd $62.64{\pm}6.783$ kg and 4-5 year old) parity were used for the trial. Feeding levels were 7.2 (I) and 5.2 (II) MJ ME/litre of milk of 3.5% fat in addition to that of the maintenance allowance. At each feeding level, diet had either unprotected (U) or formaldehyde protected (P) soya-meal. Thus, four diets were IU, IP, IIU and IIP, having six animals in each. The diets were composed of hay and pellet (10:4:1 of beet pulp : barley : soya-meal). Effect of feeding level, protein protection, parity, health status and kid number on intake, milk yield, milk composition, growth rate of goats were recorded across the 21 weeks of study. High feeding level resulted increase (p<0.001) in estimated metabolizable energy (ME) and metabolizable protein (MP) availability. Dietary inclusion of protected soya-meal increased (p<0.001) the estimated MP but not the ME availability. Animals in 1st parity ate more (p<0.001) DM (111 vs. 102 g/kg $W^{0.75}$/d) than those in 3rd parity. Animals with twin kids (110 g/kg $W^{0.75}$/d) had higher (p<0.001) DM intake than those with single kid (102 g/kg $W^{0.75}$/d). Fat (4%) corrected milk (FCM) yield was not effected by high (1,924 g/d) or low (1,927 g/d) feeding level but increased (p<0.001) with protected (2,166 g/d) compared with unprotected (1,703 g/d) soya-meal. FCM yield for four dietary combinations were 1,806, 2,078, 1,600 and 2,254 g/d for diets IU, IP, IIU and IIP, respectively. For unit increase (g) in estimated MP availability relative to ME (MJ) intake, FCM yield increased ($1,418{\pm}275.6$) g daily ($r^2$=0.58; p<0.001). Milk fat (3.14 vs. 3.54%; p<0.001) and protein (2.94 vs. 3.04% p<0.05) contents were lower at high than the low feeding level. Protected protein increased (p<0.001) the fat, lactose and net energy (NE) content of milk. Milk urea concentration of 175, 183, 192 and 204 mg/l for diets IU, IP, IIU and IIP, respectively indicated lower RDP content of these diets. The RDP contents were 6.97, 6.70, 7.30 and 6.83 g/MJ of ME for diets IU, IP, IIU and IIP, respectively. Live weight change over the experimental period were 41, 6, 17 and 19 g/d. Absence of any positive response of high feeding was probably due to inefficient rumen fermentation resulting from inadequate RDP supply. Protected protein improved production performance apparently by increasing MP:ME ratio in the absorbed nutrient.

• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.799-805
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• 1999

This study was conducted to investigate the effects of L-carnitine with different levels of lysine on performance of pigs weaned at 21 days of age. A total of 120 pigs were allotted into a $3{\times}2$ factorial design with three different levels of lysine (1.40%, 1,60% and 1.80%) and two levels of L-carnitine (0 and 1,000 ppm). Each treatment had 4 replications with 5 pigs per replicate. Pigs of $22{\pm}1$ days (5.9 kg of body weight) were grouped into a completely randomized block design. Treatments were 1) 1.4-Crt; 1.40% of lysine with 1,000 ppm of L-carnitine, 2) 1.4-N; 1.40% of lysine without L-carnitine, 3) 1.6-Crt; 1.60% of lysine with 1,000 ppm of L-carnitine, 4) 1.6-N; 1.60% of lysine without L-carnitine, 5) 1.8-Crt; 1.80% of lysine with 1,000 ppm of L-carnitine and 6) 1.8-N; 1.80% of lysine without L-carnitine. Growth performance was optimized in pigs fed 1.6% lysine regardless of carnitine addition. For the first 7 days of the experimental period, the best ADG and F/G were found in pigs within the 1.6-Crt group. Carnitine significantly improved (p<0.05) ADG of pigs when the lysine level in the diet was 1.6%. Only in the third week carnitine had a significant influence on growth performance of pigs. A lysine-sparing effect of L-carnitine was not detected in this study. The 1.6-Crt group showed the best proximate nutrient digestibility, and the crude fat and gross energy digestibility were higher when the L-carnitine was added in the diet. Lysine level significantly affected the digestibilities of DM (p<0.001), GE (p<0.001), CP (p<0.01) and C.fat (p<0.05). Carnitine also significantly improved digestibility of nutrients. Lysine level as well as carnitine level affected the amino acids digestibility, however, in 1.8% lysine diet carnitine did not influence on amino acids digestibility. Plasma carnitine content was significant higher (p<0.05) in pigs fed L-carnitine. This indicates the increased biological availability of carnitine within the body. L-carnitine supplementation tended to improve feed utilization during the third week (p<0.10) and during the entire period (p=0.10). Lysine level significantly affected feed utilization of pigs during the third week and entire period (p<0.05). As pigs grew, the lysine requirement was reduced.

• 한국해양학회지:바다
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• 제24권4호
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• pp.548-561
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• 2019

말잘피 게바다말은 우리나라 동해안의 암반조하대에 우점하는 잘피로, 이 곳 연안생태계에서 중요한 기능을 수행한다. 그러나 심한 파도에 노출된 거친 생육 환경으로 인해 지금까지 게바다말의 생태 자료는 미비한 실정이다. 본 조사에서는 동해남부해역에 서식하는 게바다말의 생태적 특성을 알아보기 위해 2017년 8월부터 2018년 7월까지 게바다말 군락지의 환경과 게바다말의 형태적 특성, 밀도, 생체량과 잎 생산성의 변화를 조사하였다. 수중광량과 수온은 봄과 여름에 증가하고 가을과 겨울에 감소하는 뚜렷한 계절 경향을 보였다. 해수의 영양염 농도는 조사 시기마다 유의한 차이를 보였으나, 뚜렷한 계절 변동은 나타나지 않았다. 게바다말의 형태, 밀도, 생체량과 잎 생산성도 가을에 감소하고 봄에 증가하였다. 게바다말의 육수화서는 2월 이후 나타났고, 5월에 최대 비율(15.8%)을 보인 후 8월 이후 탈락되었다. 게바다말의 개체당 및 단위면적당 평균 잎 생산성은 각각 2.1 mg sht^-1 d^-1과 7.5 g m^-2 d^-1이었다. 게바다말의 생장이 가장 활발한 수온은 13-14℃로 조사되었다. 게바다말의 잎 생산성은 수중광량, 수온, 영양염 농도와 유의한 상관관계가 나타나지 않았다. 이는 이 지역이 게바다말 생장에 필요한 충분한 빛, 적절한 수온 범위와 영양염 농도를 제공함을 의미한다.

• 한국토양비료학회지
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• 제16권2호
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• pp.98-105
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• 1983

토양수분조건에 따른 시비반응을 구명(究明)하기 위하여 본량(本良) 사양토(砂壤土)에서 토양수분처리($M_0$무관수, $M_1$-0.1~-1.0bar, $M_2$-0.1bar~-0.5bar, $M_3$-0.1~-0.2bar)와 시비수준(施肥水準)(N-P-Kkg/10a, 0-0-0, 11.5-10-12.5, 23-20-25, 34.5-30-37.5)을 달리하여 봄배추의 생육상태(生育狀態), 물소모량(消耗量), 양분흡수량, 비료이용율(肥料利用率), 수량 등을 조사(調査)한 결과(結果)는 다음과 같다. 1) 20cm 깊이에서 토양수분 Potential이 -2.0bar 이하(以下)일 때 물소모량은 감소(減少)하였고 비료(肥料)에 의(依)한 생장량(生長量)의 증가는 없었다. 2) 배추의 양분흡수량(養分吸收量)은 시비량(施肥量)의 증가에 따라 토양수분 Potential이 낮을 때는 감소(減少)하는 경향(傾向)이었고, 토양수분 Potential이 높을 때는 증가하는 경향(傾向)이었다. 3) 질소(窒素)와 가리(加里)의 이용율(利用率)은 토양수분 Potential과 시비수준(施肥水準)이 정비례관계(正比例關係)에 있어야 이용율(利用率)이 증대되었다. 4) 봄배추의 수량(收量)은 토양수분 Potential이 낮을 때에는 시비량(施肥量)을 증가시켜도 증수가 없었으며, 토양수분 Potential이 높을 때에는 각(各) 시비수준(施肥水準) 공(共)히 증수효과(增收效果)가 있었고 그 효과(效果)는 다비수준(多肥水準)에서 더욱 뚜렷하였다. 5) 토양수분 Potential을 높게 유지(維持)시킬 경우(境遇)에는, 시비량(施肥量)을 증가시킴에 따라 봄배추 수량(收量)이 거의 직선적(直線的)인 증가경향을 보여, 최대수량을 낼 수 있는 비료요구량(肥料要求量)이 크게 증가되었다.

• 농업과학연구
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• 제42권4호
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• pp.431-437
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• 2015

자원순환 및 친환경 농업을 실천하는 농가가 증가함에 따라 유기물을 활용한 농경지 양분관리에 대한 필요성이 대두되고 있다. 질소 공급원으로 유기물을 시용했을 때 질소 공급효과에 대한 연구들을 정리한 결과는 다음과 같다. 농경지에 유기물을 시용했을 때 질소의 공급은 질소 순환에서 무기화 과정을 통해 이루어지기 때문에 작물의 단기적인 질소 이용률은 무기질 비료에 비해 낮다. 유기물의 질소 공급량은 유기물의 C/N율이 낮을수록 무기화율이 높아지므로 C/N율을 고려한 유기물 시용이 중요하다. 가축분 퇴비의 경우 축종별 분뇨 질소함량과 퇴비와 혼합된 부산물 종류에 따라 질소 무기화율이 달라질 수 있으며, 녹비는 작물 종류와 환원시기, 토양 양분조건 등에 따라 C/N율이 달라진다. 또한 적정량의 질소를 추천하기 위해서는 토양 중 유기태 질소로부터 가용화 될 수 있는 질소의 양을 측정하여야 한다. 지금까지 보고된 퇴비, 녹비에 대한 질소공급과 작물 생산성에 대한 연구결과는 재배 여건과 작물 종류 등에 따라 다르게 나타나 질소 공급을 위한 유기물 추천 모델을 구성하기 위한 기초자료로 활용하기에는 어려움이 있다. 유기물 추천 모델을 구성하기 위해서는 우선적으로 유기물질 종류별 질소 공급량에 대한 기초 데이터가 구축되어야 하며, 토양의 질소공급량을 평가할 수 있는 분석 방법의 구축이 필요하다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1139-1146
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• 2002

Sixty crossbred Anglo-Nubian goats (growing females), 18-24 mo of age, with a mean pre-experimental live weight of $20.9{\pm}0.44kg$ were used to investigate voluntary dry matter intake (DMI) and digestibility of supplements of Leucaena leucocephala (LL) leaves to a basal diet of guinea grass. The experiment was a 3 forms of presentation: fresh, wilted and $dry{\times}4$ levels of supplementation: 10, 20, 30 and 40% of total forage allowance with LL leaves. The goats were assigned based on weight and age to one of the twelve diets in a randomized manner and each level had five animals (replicates) per diet for 56 days. The form of presentation of LL leaves had effects on dry matter (DM) and nutrient composition. DM and NDF were higher in the dry LL leaves (p<0.05). In the diets, CP increased as the level of supplementation with LL leaves increased (p<0.05). Mean CP was similar in the fresh ($14.8{\pm}3.5%$), wilted ($14.3{\pm}3.3%$) and dry forms ($13.9{\pm}3.1%$). Neutral detergent fibre (NDF) decreased linearly (p<0.05) with increase in levels of supplementation. Organic matter (OM) followed the trend of CP concentration (p<0.05). Drying was observed to reduce the availability of CP at the different levels of supplementation and subsequently this affected the NDF of the diets. DM intake of the goats on the fresh and wilted LL leaves was significantly higher (p<0.05) than in those on the dry LL leaves. DM intake of the basal diet was observed to decrease as the level of supplementation (p<0.05) increased regardless of the form of presentation of LL. Growth rate was best (p<0.05) on the fresh form followed by wilted and the least in the dry form. Growth rate decreased linearly (p<0.05) with increase in the level of supplementation in the dry form. The form of presentation and level of supplementation influenced DM, CP and OM digestibility (p<0.05). Except for NDF, the digestibility of DM, CP and OM were better (p<0.05) in the goats on fresh LL leaves compared to the wilted and dry leaves. Based on the data on DMI, growth rate and apparent nutrient digestibility coefficients, the results suggest that LL leaves is best utilized when fed fresh or wilted to goats. In the dry form of presentation, the best level of supplementation without a reduction in voluntary DMI and growth rate is the 20%. In the fresh and wilted form, LL leaves could be fed up to the 40% level with improved DMI, growth rate and nutrient utilization.

• 한국환경보건학회지
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• 제30권4호
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• pp.347-351
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• 2004

A battery of sediment bioassays was performed for the sediments from the intertidal flat zone along the middle west coast of Korea to assess their potential toxicity. In the bioassays, three crustaceans, Daphnia magna, Nitocra spines, and Hyalella aztec a were exposed to $16\%$ sediments (wet weight) collected from 14 sites. Immobility($\%$) was checked as an endpoint after 24- and 48-h exposure of Daphnia magna and after 96-h exposure of Hyalella azteca and Nitocra spines. Among the three bioassays, the 48-h Daphnia bioassay showed the most distinct differential sensitivity in relation to sediment contamination, while the Nitocra and the Hyalella bioassays failed to show the differential sensitivity properly among the sites classified as polluted. Significantly different levels of immobility ($\%$) were obtained between the sites classified as chemical/nutrient polluted and the sites classified as non-polluted in the Daphnia bioassays, but not in the Nitocra bioassay and the Hyalella bioassay. Some differences of toxic response to the same sediments among bioassays were observed, suggesting that there may be a chemical specificity of response sensitivity to sediment toxicity, due to differences in bio-availability of sediment toxicants among test species.

• ALGAE
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• 제32권2호
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• pp.161-170
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• 2017

Fourier Transform Infrared (FTIR) spectroscopy was used to study carbon allocation patterns in response to N-starvation in the nearly ubiquitous diatom Chaetoceros muellerii. The role of gene expression, protein synthesis and transamination on the organic composition of cells was tested by using specific inhibitors. The results show that inhibition of key processes in algal metabolism influence the macromolecular composition of cells and and prior cell nutritional state can influence a cell's response to changing nutrient availability. The allocation of C can thus lead to different organic composition depending on the nutritional context, with obvious repercussions for the trophic web. This also shows that C allocation in algal cells is highly flexible and that C (and the energy associated with its allocation) can be variably and rapidly partitioned in algal cells in response to relatively short term perturbations. Furthermore, the data confirm and extend the utility of infrared spectroscopy as a probe of the metabolic state of autotrophic cells.