• Journal of Microbiology and Biotechnology
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• v.30 no.4
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• pp.505-514
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• 2020

The symbiotic nature of the relationship between algae and marine bacteria is well-studied among the complex microbial interactions. The mutual profit between algae and bacteria occurs via nutrient and vitamin exchange. It is necessary to analyze the genome sequence of a bacterium to predict its symbiotic relationships. In this study, the genome of a marine bacterium, Pseudoruegeria sp. M32A2M, isolated from the south-eastern isles (GeoJe-Do) of South Korea, was sequenced and analyzed. A draft genome (91 scaffolds) of 5.5 Mb with a DNA G+C content of 62.4% was obtained. In total, 5,101 features were identified from gene annotation, and 4,927 genes were assigned to functional proteins. We also identified transcription core proteins, RNA polymerase subunits, and sigma factors. In addition, full flagella-related gene clusters involving the flagellar body, motor, regulator, and other accessory compartments were detected even though the genus Pseudoruegeria is known to comprise non-motile bacteria. Examination of annotated KEGG pathways revealed that Pseudoruegeria sp. M32A2M has the metabolic pathways for all seven vitamin Bs, including thiamin (vitamin B1), biotin (vitamin B7), and cobalamin (vitamin B12), which are necessary for symbiosis with vitamin B auxotroph algae. We also identified gene clusters for seven secondary metabolites including ectoine, homoserine lactone, beta-lactone, terpene, lasso peptide, bacteriocin, and non-ribosomal proteins.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.9
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• pp.517-525
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• 2015

This study analyzes water quality change trends in three major rivers in Daejeon and effect of discharge from Daejeon Wastewater Treatment Plant between 1992 and 2014. As of 2014, COD concentration in the Gab-cheon-A station is in level VI for Korea river water quality standard while BOD and TP are in level III. As expansions of the treatment plant, water quality in the Gab-cheon River has been improving accordingly. However, during the study period, TN concentrations of the headwater and the most downstream locations of the Gab-cheon River have increased about twice and three times, respectively. It was estimated that the treatment plant is responsible for 35%. 46%, 76% and 63% of BOD, COD, TN and TP loadings of the Gab-cheon River, respectively. It was also estimated that small tributaries and nonpoint sources are responsible for 54% and 47% of BOD and COD loadings of the Gab-cheon River. Therefore, it is recommended to further reduce nutrient loadings from the treatment plant and also reduce surface runoff organic loading from nonpoint sources including small tributaries and storm sewers.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.247-251
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• 2008

During a 35-day growth cycle, N, P, and K uptake was determined by measuring changes in their contents in culture solutions. At harvest, plants were separated into the roots, base organs and shoot, and dried for tissue analysis for N, P, and K. The uptake rates of N, P, and K followed cyclical patterns that was related to shoot development and harvest, but were independent of the transpiration rate. Uptake of N declined from 5.6 mmol $plant^{-1}$ $day^{-1}$ just prior to the cycle initiation to 4.0 mmol $plant^{-1}$ $day^{-1}$ at day 15. Uptake rate steadily increased as flower stems reached maturity up to 10.3 mmol $plant^{-1}$ $day^{-1}$ day 35. Uptakes rates of P and K followed similar patterns of N uptake. Tissue concentrations of N and P steadily decreased since day 15. Content of K was the lowest at day 20 and steadily increased thereafter. In the root tissue, N and K contents were the lowest at day 15, increased to day 30, and then decreased at day 35. Tissue P content was just a reverse of those of N and K.

• Journal of Nutrition and Health
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• v.34 no.4
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• pp.458-471
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• 2001

To evaluate the infra structure supporting hospital nutrition services, we conducted a survey on the unit of organization, unit of dietitians work system, number of personnel engaged on nutrition services, productivity of food service, management of dietitians works, computerization of nutrition services etc. Total ninety-six hospitals were participated in the survey, and they were varied in terms of hospital classification, location, number of beds and type of food service management. All of the large hospitals with more than 400 beds conducted nutrition services under the department of nutrition, but some of the middle and small hospitals with less than 400 beds conducted nutrition services under the other department such as administration. In most of the tertiary hospitals, the work of dietitians were separated in which food services and medical nutrition services were conducted independently by different dietitians, whereas, in most of general hospitals and all the hospital, food services and medical nutrition services were conducted by the same dietitians in all time. The numbers of dietitians and cooks per 100 beds were fewer in the large hospitals with more than 400 beds than the hospitals with less than 400 beds, and the number of cooking and meal serving assistants were the just opposit. The average productivity of food service was 44.5 meals per hour for each dietitian, 84.8 meals per hour for a cook and 7.0 meals per hour for a cooking and meal serving assistant. The productivities for dietitians and cooks tend to be higher in large hospitals than middle and small hospitals, whereas the productivities for cooking and meal serving assistants were just opposite. The large hospitals seemed to solve the problem on the lack of working personnels by hiring part-time workers and by utilization of computer system for their works. The pattern of daily work management in food service area was not much different between dietitians duties, but the pattern of daily work management in medical nutrition service area was different in a way which the analysis of patients nutrient intakes was almost not conducted by dietitians handling both food services and medical nutrition services. Therefore, this study demonstrates that there are significant differences in the infra structures conducting nutrition services among hospitals, suggesting that the strategies to improve this improve this structure in relation to the improvement of service qualities need to be investigated in the future. (Korean J Nutrition 34(4) : 458∼471, 2001)

• Journal of Applied Biological Chemistry
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• v.54 no.1
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• pp.1-6
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• 2011

${\gamma}$-PGA(poly-${\gamma}$-glutamic acid) is an unusual anionic polypeptide that is made of D- and L-glutamic acid units connected by amide linkages between ${\alpha}$-amino and ${\gamma}$-carboxylic acid groups. ${\gamma}$-PGA has been isolated from many kinds of organisms. Many Bacillus strains produce ${\gamma}$-PGA as a capsular material of an extracellular viscous material. It is safe for eating as a viscosity element of fermented soybean products such as Chungkookjang and Natto. It is biodegradable, edible and nontoxic toward humans and the environment and its molecular weight varies from ten thousand to several hundred thousand depending on the kinds of strains used. Therefore, potential applications of ${\gamma}$-PGA and its derivatives have been of interest in the past few years in a broad range of industrial fields such as food, cosmetics, medicine, water-treatment, etc. In this study, a bacterium, Bacillus subtilis GS-2 isolated from the Korean traditional seasoning food, Chungkookjang could produce a large amount of ${\gamma}$-PGA with high productivity and had a simple nutrient requirement. Based on carbon utilization pattern and partial 16S rRNA sequence analysis, the GS-2 strain was identified as B. subtilis. The determination of purified ${\gamma}$-PGA was confirmed with thin layer chromatography (TLC), high performance liquid chromatography (HPLC), fourier transform infrared (FT-IR) spectra, and $^1H$-nuclear magnetic resonance ($^1H$-NMR) spectroscopy.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.7
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• pp.418-425
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• 2015

Anaerobic Filters (AF) packed with porous ceramic floating media were operated at different operational conditions to identify the feasibility of the renewable bioenergy, methane production from swine wastewater and to verify the suitability of effluent from anaerobic filters for the subsequent biological nitrogen and phosphorus removal. Stepwise increase in organic loading rates (OLRs) or decrease in hydraulic retention times (HRTs) with influent TCOD concentration of 14,000 mg/L were utilized at mesophilic temperature. The maximum methane productivity of 1.74 volume of $CH_4$ per volume of reactor per day (v/v-d) was achieved at an hydraulic retention time (HRT) of 0.5 day (OLR 28 g TVS/L-d). Based on the biogas production, the highest total volatile solids (TVS) removal efficiency of 63% was obtained at an HRT of 3 days (OLR 4.67 g TVS/L-d), however based on the result from the effluent total chemical oxygen demand (TCOD) analysis, the highest TCOD removal efficiency of 75% was achieved. The effluent alkalinity concentration over the range of 2,050~2,980 mg/L as $CaCO_3$ at all operational conditions, could compensate the alkalinity destruction caused by nitrification. The effluent from the anaerobic filter operated under the HRT of 2 days showed the COD/TKN ratio of 15~35 and COD/TP ratio of 38~56. Therefore effluent C/N/P ratio is able to satisfy the optimum COD/TKN ratio of greater than 8.0 and COD/TP ratio of 33 for the subsequent biological nutrient removal.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.245-251
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• 1998

A 10-week feeding experiment was conducted to determine the influence of several minerals from dietary mineral premix on growth and body composition in juvenile Korean rockfish. Three replicate groups of fish Initially averaging 4.2 g were fed one of the ten experimental diets. Korean rockfish muscle and casein as protein sources were used in the basal diet, and deleted one of each mineral (P, Ca, Zn, Mg, Fe, K, Mn, Se or all) in the control mineral premix, Weight gain, feed efficiency and nutrient retention in fish fed the diet not containing mineral premix were significantly lower than those in fish fed other diets (P<0.01). Weight gain in fish fed the diets deleted each mineral (P, Ca, Zn, Hg, Fe, K, Mn or Se) was lower than that of control diet (P<0.01), Feed efficiency and protein retention in fish fed the diets deleted each of P, Ca, Zn or Mn were lower than those ot control diet (P<0.01). Daily feed intake and lipid retention were not affected by dietary mineral premixes ( P>0.01). Proximate analysis and mineral contents in the whole body, muscle, liver or bone of fish fed the diets deleted each mineral were not different to control diet ( P>0.01). The data obtained in this study indicate that each of the P, Ca, Zn, Mg, Fe, K, Mn or Se is essential for normal growth of Korean rockfish.

• Journal of Nutrition and Health
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• v.6 no.4
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• pp.15-22
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• 1973

Sodium chloride plays an important role as the main condiment at daily meal. It is well known that humans require sodium chloride as an essential nutrient to keep the homeostasis of electrolytes. The amounts of salt intake may be a reflection of geography, culture and food habit rather than necessity. Lee has reported (1962) that Koreans ingest high amounts of sodium chloride in their meals, with an intake of excess carbohydrate (80-90% of total Calories) and low protein in their diet. This includes large amounts of rice, Kimchi and other fermented soybean products common in the Korean diet. This investigation was designed to study the dietary relations of sodium chloride to other nutrients in the Korean diet. Twenty four albino male rats, weighing from 290-300g, were divided into four dietary groups according to the amounts of carbohydrate, protein and fat in the basal diet. Each diet contained a rice powder as a carbohydrate source. Diet I was a control diet, Diet II, low protein, Diet III, low protein and low fat diet and Diet IV, low fat diet. All rats were provided with 3% sodium chloride solution. Diet and salt solution were given ad libitum. The experiment was carried out for 9 weeks during which time the body weight, the food intake, and 3% sodium chloride solution consumption were determined. At the 9th week, the urine was collected the blood sample from the artery of each rat for the analysis of sodium and potassium and other chemical studies. The rats were sacrificed and the kidney, adrenal, liver and spleen were measured, and observed changes of the pathological tissue in the kidney and adrenal. The results were summarized as follows: 1) The growth rate was higher in Diet I than in the other experimental diets (II, III and IV) after 4 weeks. There was no significant difference found between the experimental Diets II, III and IV. 2) The daily food intake was greater in the experimental diets II, III and IV than in the control diet. However, there was no difference among the high carbohydrate diets Diet II, III and IV. 3) The daily water (3% sodium chloride solution) intake was also greater in the Diets II, III and IV, than in the control diet. However, there was no difference between Diets II, III and IV. 4) The concentration of sodium and potassium in the blood were within the normal range in all diets. 5) The amount of sodium chloride in the urine was significantly greater in Diets II, III and IV than in the control diet. Diets II, III, IV had a larger amount of sodium solution consumption. 6) Observation of pathological tissue in the experimental diets found a cell proliferation in the glomerlulus of the kidney, while such change was not found in the control diet.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1119-1127
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• 2019

This study analyzes the content of niacin, B1, and B2, which are among the water-soluble vitamin B group, in cultivars of the commonly consumed agricultural products of apples, peaches, nectarines and strawberries to compare content differences and to use results as base material for the Korean Food Composition Table. While the vitamin B1 content of apples according to different cultivars was found to be within the ranges of 0.063-0.208 mg/100g, and the content of vitamin B2 was found to be within the value ranges of 0.006-0.031 mg/100g, no niacin was found. The vitamin B1 content of peaches and nectarines according to different cultivars was found to be within the value ranges of 0.014-0.276 mg/100g, the content of vitamin B2 was found to be within he value ranges of 0.019-0.042 mg/100g, and niacin content was found to be within the value ranges of 0.298-1.096 mg/100g. The vitamin B1 content of strawberries according to cultivars was found to be within the value ranges of 0.112-0.394 mg/100g, the content of vitamin B2 was found to within the value ranges of 0.001-0.027 mg/100g, and niacin content was found to be within the value ranges of 0.388-0.809 mg/100g. Therefore, when nutrient composition analysis databases for the fruits of apples, peaches, and strawberries are constructed, cultivar factors must be put into consideration. In addition, differences can be found according to fruit harvest times, cultivation methods, and environmental factors, so related additional is needed.

• Journal of Nutrition and Health
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• v.45 no.2
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• pp.150-158
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• 2012

This study was conducted to investigate the relationship between knowledge of nutrition labeling and the effect on eating habits with 300 high school boys in Seoul. The subjects were divided into an underweight (UW) group (BMI < $18.5kg/m^2$, n = 42), a normal weight (NW) group ($18.5kg/m^2\;{\leq}\;BMI\;<\;23kg/m^2$, n = 129) and an overweight (OW) group (BMI ${\geq}\;23kg/m^2$, n = 79) based on their body mass index (BMI). The average age of participants was 17.8 years old and their mean height and weight were 174.9 cm and 66.5 kg. The mean BMI of subjects was $21.7kg/m^2$ which fell within the normal range. Seventy six point four percent of subjects perceived nutrition labeling and they acquired the information pertaining to it through TV and internet. The UW group and the OW group obtained it from their parents, relatives and friends, while NW group acquired it from school. There were significant differences among groups in the acquired source of the information on nutrition labeling (p < 0.05). The NW group and the OW group trusted nutrition labeling more than the UW group, but there were no significant differences among them. Forty five point five percent of the UW group and 40.7% the NW group were satisfied with nutrition labeling education, while only 15.8% of the OW group did it. The OW group checked nutrition labeling more than the UW group and the NW group at the point of food purchase. The primary reason for examining nutrition labeling was 'to check nutrient contents' in the UW group and the NW group, while the OW group examined it to improve health including regulation of body weight. There were significant differences among groups with regards to the reason for examining nutrition labeling (p < 0.001). The OW group was aware that nutrition labeling affected their eating ha-bits significantly more than the other groups (p < 0.05). Therefore, application-centered education on nutrition labeling and the strong support of the government is needed in order to improve nutrition labeling use and to apply the information from nutrition labeling into student dietary life.