• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.6
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• pp.121-128
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• 2011

Long-term water treatment performance analysis was conducted for the constructed wetland treating agricultural tailwater. Studied wetland was established in 2001 and operated from June, 2002 to November, 2010. Wetland vegetation cover was shown over 90 % coverage after 2005. According to vegetation development, accumulation of sediment nutrient was observed; Organic Carbon, T-P and T-N. In addition, DO concentration and temperature was decreased in the constructed wetland output. An infiltration rate also significantly decreased due to compaction of wetland soil. A runoff coefficient was increased due to the low infiltration rate after 2005. A T-N, TSS and Chl.a removal rate was maintained constantly. However, the T-P removal rate was slightly decreased along to wetland operation because low DO concentration could increase elution of phosphorus from sediment. After constructing open water, the T-P removal rate was increased. This is because open water could accelerate the reaearation process. Consequently, over three years of vegetation development could be helpful for wetland performance. In addition, DO concentration is important factor to maintain the T-P treatment.

• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.8-13
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• 1998

Animal cells in culture typically convert most of the glucose they consume into lactate. The accumulation of lactate, however, is commonly cited as one of the factors that inhibit cell growth and limit the maximum cell concentration that can be achieved in culture. The specific production of lactate and the amount of glucose converted to lactate can be reduced when cells are grown in a fed-batch culture in which the residual glucose concentration is maintained at low levels. Such a fed-batch culture was used to grow and adapt hybridoma cells into a low-lactate-producing state before changing into continuous culture. The cells reached and maintained a high viable cell concentration at steady state. In a similar manner, cells that were initially grown in batch culture and a glucose-rich environment reached a steady state with a cell concentration that is much lower. The feed composition and dilution rates for both cultures were similar, suggesting steady state multiplicity. From a processing perspective the desired steady state among those is the one with the least metabolite production. At such seady state nutrient concentration in the feed can be further increased to increase cell and product concentrations without causing the metabolite inhibitory effect typically seen in a cell culture. Controlling cell metabolism in a continuous culture to reduce or eliminate waste metabolite production may significantly improve the productivity of mammalian cell culture processes.

• Fisheries and Aquatic Sciences
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• v.12 no.4
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• pp.317-323
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• 2009

Waste products were biologically estimated from juvenile flounder (Paralichthys olivaceus) on three diet types-raw fish-based moist pellets, moist pellets, and extruded pellets. Total solid and soluble wastes were estimated by determining nutrient digestibility and accumulation in juvenile flounders through growth trials. Total solid wastes produced were 20%-23% of the organic matter supplied. Soluble excretions ranged from 45% to 49%. Soluble nitrogenous excretions ranged from 36.4% to 46.2%. These results indicate that about 30.2%-35.9% of supplied feed is retained in the fishes' bodies while the remainder of feed is excreted into culture systems or the surrounding environment.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.10a
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• pp.15-20
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• 2005

Locations of silicon accumulation in rice leaves and its possible association with resistance to rice blast were investigated by analytical electron microscopy and atomic force microscopy. A blast-susceptible cultivar, Jinmi, and partially resistant cultivars, Hwaseong and Suwon345, were grown under a hydroponic culture system with modified Yoshida's nutrient solution. Electron-dense silicon layers were frequently found beneath the cuticle in epidermal cell walls of silicon-treated plants. Increasing levels of silicon were detected in the outer regions of epidermal cell walls. Silicon was present mainly in epidermal cell walls, middle lamella, and Intercellular spaces within subepidermal tissues. Furthermore, silicon was prevalent throughout the leaf surface with relatively small deposition on stomatal guard cells in silicon-treated plants. Force-distance curve measurements revealed relative hardness and smaller adhesion force in silicon-treated plants (18.65 uN) than control plants (28.39 uN). Moreover, force modulation microscopy showed higher mean height values of elastic Images In silicon-treated plants(1.26 V) than in control plants (0.44 V), implying the increased leaf hardness by silicon treatment. These results strongly suggest that silicon-induced cell wall fortification of rice leaves may be closely associated with enhanced host resistance to blast.

• Korean Journal of Poultry Science
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• v.25 no.3
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• pp.137-145
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• 1998

This study was conducted to compare the meat characteristics Korean Native Commercial Chicken(KNCC) by feeding system Feeding system of T$_1$ and T$_2$were same types from hatch to 8 weeks, starter diets( 0~4 weeks, mash, ME 3,l00kcal, CP 22.94%), grower diets(4~8 weeks, crumble, ME 3,l00kcal, CP 19.31%). Nutrient contents of finisher diets of T$_1$(pellet, M.E 3, 200kcal, CP 20.44%) was higher than T$_2$(mash, ME 3,l00kcal, CP 14.88%). Breast and legs meat of KNCC were used to analyze chemical composition, physicochemical characteristics, textural traits and sensory evaluation test. Live weight(L.W) /abdominal fat accumulation(A.F.A) was significantly different(P<0.05), but live weight(L.W) /dressed weight(D.W), percentage of cutted meat, chemical composition(moisture, CF, EE, CA), meat color(CIE), physicochemical properties, sensory evaluation score were not significantly by feeding systerns, at 12 ar'd 16 weeks of age.

• BMB Reports
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• v.46 no.12
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• pp.606-610
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• 2013

Glucagon like peptide-1 (GLP-1) regulates glucose mediated-insulin secretion, nutrient accumulation, and ${\beta}$-cell growth. Despite the potential therapeutic usage for type 2 diabetes (T2D), GLP-1 has a short half-life in vivo ($t_{1/2}$ <2 min). In an attempt to prolong half-life, GLP-1 fusion proteins were genetically engineered: GLP-1 human serum albumin fusion (GLP-1/HSA), AGLP-1/HSA which has an additional alanine at the N-terminus of GLP-1, and AGLP-1-L/HSA, in which a peptide linker is inserted between AGLP-1 and HSA. Recombinant fusion proteins secreted from the Chinese Hamster Ovary-K1 (CHO-K1) cell line were purified with high purity (>96%). AGLP-1 fusion protein was resistant against the dipeptidyl peptidase-IV (DPP-IV). The fusion proteins activated cAMP-mediated signaling in rat insulinoma INS-1 cells. Furthermore, a C57BL/6N mice pharmacodynamics study exhibited that AGLP-1-L/HSA effectively reduced blood glucose level compared to AGLP-1/HSA.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1958-1965
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• 2008

A denitrifying polyphosphate-accumulating bacterium (YKP-9) was isolated from activated sludge of a 5-stage biological nutrient removal process with step feed system. This organism was a Gram-negative, coccus-shaped, facultative aerobic chemoorganotroph. It had a respiratory type of metabolism with oxygen, nitrate, and nitrite as terminal electron acceptors. The 16S rRNA gene sequence of strain YKP-9 was most similar to the 16S rRNA gene sequence of Paracoccus sp. OL18 (AY312056) (similarity level, 97%). Denitrifying polyphosphate accumulation by strain YKP-9 was examined under anaerobic-anoxic and anaerobic-oxic batch conditions. It was able to use external carbon sources for polyhydroxyalkanoates(PHA) synthesis and to release phosphate under anaerobic condition. It accumulated polyphosphate and grew a little on energy provided by external carbon sources under anoxic condition, but did neither accumulate polyphosphate nor grow in the absence of external carbon sources under anoxic condition. Cells with intracellular PHA cannot accumulate polyphosphate in the absence of external carbon sources under anoxic condition. Under oxic condition, it grew but could not accumulate polyphosphate with external carbon sources. Based on the results from this study, strain YKP-9 is a new-type denitrifying polyphosphate-accumulating bacterium that accumulates polyphosphate only under anoxic condition, with nitrate and nitrite as the electron acceptors in the presence of external carbon sources.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.2
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• pp.129-133
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• 1999

Excessive soil water at vegetative growth stages during the rainy season induces yield losses in soybeans. Our objectives were to obtain basic information about the cultivar differences and to understand the stress-tolerance process for due to excessive soil water. Previous experiments revealed soybean genotypic differences in tolerance to excessive soil water. A field experiment was conducted at the Research Farm of Korea University near Seoul on 21 May 1998. Soybean[Glycine max (L.) Merrill] cultivars, 'Hannamkong' (sensitive) and 'Taekwan-gkong'(tolerant) were planted in vinyl-lined plots(1.2 x 4.2 x 0.3 m deep) and control plots. Drip irrigation began at VI growth stage to submerge the soil surface. Three weeks of excessive soil water treatment reduced all growth parameters measured to soybean plants. Excessive soil water stress resulted in decreases of N, P, K, Ca, Mg and Cu, and increases of Fe and Mn contents in soybean leaves. The stress index of tolerant cultivars under excessive soil water showed no large difference in soybean growth characteristics measured at three growth stages. However, K, Ca, Mg, Fe and Mn contents in soybean leaves appeared to differ between sensitive and tolerant cultivars. From the above results, stress and tolerance indices are proposed for a method to test cultivar differences in plant responses within a species under adverse growth environments.

• Ocean and Polar Research
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• v.32 no.3
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• pp.291-297
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• 2010

In the summer of 2008 (August 4-14), vertical and horizontal distributions of inorganic nutrients and dissolved organic carbon (DOC) were measured in the southwestern East Sea. Concentrations of DOC were determined for the first time in the southwestern East Sea using the high-temperature combustion oxidation (HTCO) method, and results were compared with those measured by another laboratory. Concentrations of DOC ranged from 58 to 104 ${\mu}M$ in the upper 200 m, showing a typical decreasing pattern with depth. Generally, concentrations of DOC were relatively lower, with higher nutrient concentrations, in the upper layer of the coastal upwelling zone. Concentrations of DOC ranged from 54 to 64 ${\mu}M$ in the deep Ulleung Basin (200-1500 m), and were higher than those in the Pacific and Atlantic oceans. In association with rapid vertical ventilation of the euphotic, this difference indicates a larger accumulation of semi-labile DOC in the deep East Sea than in the major oceans. A correlation between apparent oxygen utilization (AOU) and DOC in the deep ocean of the East Sea revealed that only a small portion (<10%) of the sinking DOC, relative to the sinking particulate organic carbon (POC), contributes to microbial degradation. Our results present an important data set of DOC in the East Sea, which plays a critical role in carbon cycle modeling and sequestration.

• Magazine of the Korean Society of Agricultural Engineers
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• v.42 no.3
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• pp.66-75
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• 2000

Effect of reclaimed sewage on the paddy rice culture was examined by field experiment for two consecutive years. The domestic sewage was treated by the constructed wetland and the effluent of the treatment wetland was used for irrigation water. The reclaimed sewage was diluted before irrigation in the first year and it was used without dilution in the second year experiment. Growth components and yields were compared against the control plot where conventional method was applied. And also soil characteristics of the plots before and after reclaimed sewage irrigation were analyzed Generally addition of the reclaimed sewage irrigation didn't affect paddy rice culture adversely and even enhancement was observed. Fertilization was thought to be important factor for rice culture rather than irrigation water quality. Conventional fertilization and reclaimed sewage irrigation which contained high nutrient concentration resulted in better growth and more yield. Unlike widespread concern lodging did not happen even in the case of irrigation with average 90mg/L of T-N and conventional fertilization. Soil characteristics changed after irrigation and significant EC increasing was observed for the reclaimed sewage irrigation plots. From soil analyses salt accumulation could be a more potential problem than nutrients like nitrogen and phosphorus in the use of reclaimed sewage irrigation. Overall reclaimed sewage irrigation was thought to be one of practical alternatives for the ultimate disposal of sewage in rural area.