• 한국식품영양과학회지
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• 제19권4호
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• pp.335-341
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• 1990

Nuclei proteins were purified from chick liver to homogeneity by means of acid extraction CM Sephadex c 25 column chromatography and Bio Rex 70 column chromatography, The molecular weight of liver Nuclei proteins 1 and 2 as estimated by electrophoresis on SDS-polycrylamide gel are 29000 and 27,000 respectively. These molecular weights are identical with those of Nuclei Proteins 1 and 2 isolated from chick erythrocyte. The liver and erythrocyte Nuclei Proteins also co-migrated in acetic acid-urea gel electrophoresis. Furthermore the anti-sera raised against liver Nuclei Proteins 1 and 2 cross-reacted with erythrocyte Nuclei Proteins 1 and 2 respectively, However the amino acid compositions of liver Nuclei Prooteins 1 and 2 were found to be different from those of corresponding erythrocyte Nuclei proteins ; the contents of serine and proline in liver Nuclei proteins were higherocyte Nuclei proteins ; the contents of serine and proline in liver Nuclei protesins were higher than those in erythrocyte Nuclei proteins while the content of lycsine in liver Nuclei proteins was lower than the erythrocyte Nuclei proteins, These results suggest that in spite of similarities in many respects the liver and erythrocyte Nuclei proteins in chicks and different proteins.

• 천문학논총
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• 제8권1호
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• pp.179-183
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• 1993

We analyze the evolution of active galactic nuclei for the decreasing accretion rate case. Our analysis is based on the unified theory of active galactic nuclei which entirely depends on the accretion rates of the central supermassive black holes. Our discussion leads us to conclude that active galactic nuclei may evolve from QSOs into the nuclei of Seyfert or radio galaxies.

• 한국가축번식학회지
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• 제14권3호
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• pp.205-211
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• 1990

Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell mouse embryos by micromanipulation and sendai virum mediated fusion. no significant difference in successful injectin rate and fusion rate was found between the cell stages of nuclear donor embryos. There nuclear transplant embryos receiving different cell stage nuclei were cultured in vitro for 96 hours. 75.3% of 255 embryos receiving 2-cell nuclei, 68.2% of 236 embryos reciving 4-cell nuceli and 46.9% of 228 embryos receiving 8-cell nuclei were developed to blastocyst, respectively. The number of blastomeres was significantly(P<0.05) reduced in the embryos receiving 8-cell nuclei, compared with the embryos receiving 2-cell, 4-cell nuclei or the intact embryos. Also the size of blastocysts was significantly(P<0.05) smaller in the embryos receiving 8-cell nuclei, compared with the intact or other nuclear transplant embryos. These results suggest that single nuclei introduced into the enucleated two-cell embryos are able to support the in vitro development of the reconstituted embryos to blastocysts. The prominant retardation of blastocoele formation and cell division was shown in nuclear transplant embryos receiving eight-cell nuclei when they were cultured in vitro.

• 정보처리학회논문지B
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• 제16B권2호
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• pp.131-140
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• 2009

본 논문에서는 입력된 FISH 세포영상을 군집세포영역과 독립세포영역으로 분류하고, 군집세포영역에 대해서는 하나의 세포를 분리하는 알고리즘을 제안한다. 먼저 입력된 영상에 대해서 가우시안혼합모델과 세포의 명암도 값에 대한 최대 우도 함수를 사용하여 세포영역과 배경영역을 분할해줄 임계값을 정의하게 된다. 이렇게 얻어진 전경세포영역에 대해서 보다 정확한 세포 분석을 위해서 군집세포와 독립세포를 분류하게 된다. 세포 영역의 분류과정을 위해서는 베이지안 네트워크와 확률밀도함수를 사용한다. 학습데이터로부터 밀집도(compactness), 평활도(smoothness), 후-모멘트(Hu-moment)에 대한 형태학적 특징값을 추출하여 확률밀도함수를 구성하고, 이를 기반으로 베이지안 네트워크를 사용하여 두 영역을 분류하게 된다. 군집세포로 분류된 영역에 대해서는 그 군집세포를 구성하고 있는 독립세포로 각각 분리한다. 먼저, 명암도 기울기 변환(intensity gradient transform) 영상과 워터쉐드 알고리즘을 이용하여 군집세포 영역을 작은 영역으로 분할하게 된다. 작게 분할된 영역을 하나의 세포영역으로 병합시키기 위해서, 군집세포에 존재하는 독립세포의 수만큼의 마커를 결정 침식 연산을 사용하여 추출하고, 추출된 마커를 중심으로 단계적 병합 알고리즘을 제안한다. 본 논문에서 제안한 방법은 166개의 FISH 세포를 사용하여 테스트한 결과 99.29%의 정확한 분리결과를 보여줬으며 기존의 다른 알고리즘보다도 뛰어난 성능과 빠른 실행시간을 보여주었다.

• Animal cells and systems
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• 제2권1호
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• pp.71-80
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• 1998

Glutamate dehydrogenase (GDH) is one of the main enzymes involved in the formation and metabolism of the neurotransmitter glutamate. In the present study, we investigated the distribution of the GDH-immunoreactive cells in the rat brain using monoclonal antibodies against bovine brain GDH isoprotein. GDH-immunoreactive cell were distributed in the basal ganglia, thalamus and the nuclei belong to substantia innominata, and its connecting area, subthalamic nucleus, zona incerta, and substantia niqra. We could see GDH-immunoreactive cells in the hippocampus, septal nuclei associated with the limbic system, the anterior thalamic nuclei connecting between the hypothalamus and limbic system, and its associated structures, amygdaloid nuclear complex, the dorsal raphe and median raphe nuclei and the reticular formation of the midbrain. The GDH-immunoreactive cells were shown in the pyramidal neurons of the cerebral cortex, the Purkinie cells of the cerebella cortex, their associated structures, ventral thalamic nuclei and the reticular thalamic nuclei that seem to function as neural conduction in the thalamus.

• International Journal of Computer Science & Network Security
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• 제22권3호
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• pp.273-275
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• 2022

Deep Learning is used nowadays in Nuclei segmentation. While recent developments in theory and open-source software have made these tools easier to implement, expert knowledge is still required to choose the exemplary model architecture and training setup. We compare two popular segmentation frameworks, U-Net and Mask-RCNN, in the nuclei segmentation task and find that they have different strengths and failures. we compared both models aiming for the best nuclei segmentation performance. Experimental Results of Nuclei Medical Images Segmentation using U-NET algorithm Outperform Mask R-CNN Algorithm.

• 천문학회지
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• 제44권5호
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• pp.195-200
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• 2011

We have revisited the ACS Virgo Cluster Survey (ACSVCS), a Hubble Space Telescope program to obtain ACS/WFC g and z bands imaging for a sample of 100 early-type galaxies in the Virgo Cluster. In this study, we examine 51 nucleated early-type galaxies in the ACSVCS in order to look into the relationship between the photometric and structural properties of stellar nuclei and their host galaxies. We morphologically dissect galaxies into five classes. We note that (1) the stellar nuclei of dwarf early-type galaxies (dS0, dE, and dE,N) are generally fainter and bluer with g > 18.95 and (g-z) < 1.40 compared to some brighter and redder counterparts of the ellipticals (E) and lenticular galaxies (S0), (2) the g-band half-light radii of stellar nuclei of all dwarf early-type galaxies (dS0, dE, and dE,N) are smaller than 20 pc and their average is about 4 pc, and (3) the colors of red stellar nuclei with (g - z) > 1.40 in bright ellipticals and lenticular galaxies are bluer than their host galaxies colors. We also show that most of the unusually "red" stellar nuclei with (g-z) > 1.54 in the ACSVCS are the central parts of bright ellipticals and lenticular galaxies. Furthermore, we present multi photometric band color - color plots that can be used to break the age-metallicity degeneracy particularly by inclusion of the thermally pulsing-asymptotic giant branch (TP-AGB) phases of stellar evolution in the stellar population models.

• 한국균학회지
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• 제24권1호통권76호
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• pp.1-7
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• 1996

느타리버섯균의 이핵체 체세포 균사에서 분열전 및 분열후의 핵과 분열중의 핵을 화학고정방법과 동결교체방법을 이용하여 균사분화과정중의 성상체 미세소관의 미세구조적 동태를 조사하였다. 많은 수의 성상체 미세소관들이 이동중인 분열전의 핵들과 연관되어 있었으며, 분열전의 핵이 이동하는 시기에 SPB의 분리가 진행됨이 발전되었다. 분열전 이동중의 핵과는 대조적으로 SPB의 분리가 더 이상 일어나지 않는 분열중의 이거나 분열이 끝난 핵에서는 성상체미세소관이 덜 발달되어 있었다. 활발히 이동할 것으로 추측되는 분열후의 핵에서 핵과 연관된 성상체미세소관이 잘 발달되어 있지 않음은 특이한 점이다. 분열중의 모든 핵에서 성상체미세소관이 SPB에서 뻗어나오며 인은 핵막에 남아있다. 핵의 분열과 핵의 이동 및 SPB의 분리와 관련된 성상체미세소관의 기능에 대하여 논의하였다.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.129-134
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• 2000

Clear visualization of pepper (Capsicum annuum L.) microspore nuclei with common stains such as acetocarmine or propionocarmine is difficult, hindering cytological analysis. The DAPI stain after the addition of ferric chloride solution to fixative resulted in clear visualization of nuclei. For clear visualization of nuclei and slight fluorescence of microspore wall, addition of 40-60 ${mu}ell$ of ferric chloride solution to the 1 $m\ell$ fixative was identified as most effective. At all stages of gametophytic development, the nuclei can be distinctly visualized. Starch granules does not intefere with the fluorochrome, and so the vegetative and generative nuclei were cleary visible in binucleate pollens. With its rapidity and reliability, this technique represents an efficient tool for routine staging or investigation of the nuclear status of the microspore during culture.

• Animal cells and systems
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• 제9권3호
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• pp.145-152
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• 2005

A retrograde tracer, WGA-apo-HRP-gold, was injected into midline thalamic nuclei and subsequently orexin-A immunostaining was performed on the tuberal region of the hypothalamus in order to investigate orexinergic projections to the midline thalamus. Injection site was targeted within one specific region, i.e., paraventricular, centromedian, rhomboid, reuniens, or intermediodorsal nucleus, but it proved to be either one or a combination of these thalamic nuclei. The distribution of WG/orexin-double-labeled neurons exhibited a general pattern in that the majority of labeled cells were observed within the ventral portion of the lateral hypothalamus as well as the perifornical nucleus (PeF). A small number of double-labeled cells were also observed at the dorsomedial nucleus, the area dorsal to the PeF, dorsal portion of the lateral hypothalamus, and the posterior hypothalamus. These orexin-immunoreactive neurons might have wake-related influences over a variety of functions related with midline thalamic nuclei, which include autonomic control, associative cortical functions, and limbic regulation.