• Proceedings of the KSAR Conference
• /
• 2001.10a
• /
• pp.37-43
• /
• 2001

1. About fifty thousand of cattle embryos were transferred and 16000 ET-calves were born in 1999. Eighty percents of embryos were collected from Japanese Black beef donors and transferred to dairy Holstein heifers and cows. Since 1985, we have achieved in bovine in vitro fertilization using immature oocytes Collected from ovaries of slaughterhouse. Now over 8000 embryos fertilized by Japanese Black bull, as Kitaguni 7 -8 or Mitsufuku, famousbulls as high marbling score of progeny tests were sold to dairy farmers and transferred to their dairy cattle every year. 2. Embryo splitting for identical twins is demonstrated an useful tool to supply a bull for semen collection and a steer for beef performance test. According to the data of Dr.Hashiyada (2001), 296 pairs of split-half-embryos were transferred to recipients and 98 gave births of 112 calves (23 pairs of identical twins and 66 singletons). 3. A blastomere-nuclear-transferred cloned calf was born in 1990 by a joint research with Drs.Tsunoda, National Institute of Animal Industry (NIAI) and Ushijima, Chiba Prefectural Farm Animal Center. The fruits of this technology were applied to the production of a calf from a cell of long-term-cultured inner cell mass (1998, Itoh et al, ZEN-NOH Central Research Institute for Feed and Livestock) and a cloned calf from three-successive-cloning (1997, Tsunoda et al.). According to the survey of MAFF of Japan, over 500 calves were born until this year and a half of them were already brought to the market for beef. 4. After the report of "Dolly", in February 1997, the first somatic cell clone female calves were born in July 1998 as the fruits of the joint research organized by Dr. Tsunoda in Kinki University (Kato et al, 2000). The male calves were born in August and September 1998 by the collaboration with NIAI and Kagoshima Prefecture. Then 244 calves, four pigs and a kid of goat were now born in 36 institutes of Japan. 5. Somatic cell cloning in farm animal production will bring us an effective reproductive method of elite-dairy- cows, super-cows and excellent bulls. The effect of making copy farm animal is also related to the reservation of genetic resources and re-creation of a male bull from a castrated steer of excellent marbling beef. Cloning of genetically modified animals is most promising to making pig organs transplant to people and providing protein drugs in milk of pig, goat and cattle.

• The Journal of Engineering Geology
• /
• v.26 no.2
• /
• pp.235-250
• /
• 2016

In Korea, 70% of the annual rainfall falls in summer, and the number of days of extreme rainfall (over 200 mm) is increasing over time. Because rainfall is the most important trigger of landslides, it is necessary to decide a rainfall threshold for landslide warning and to develop a landslide warning model. This study selected 12 study areas that contained landslides with exactly known triggering times and locations, and also rainfall data. The feasibility of applying a Rainfall Triggering Index (RTI) to Korea is analyzed, and three RTI models that consider different time units for rainfall intensity are compared. The analyses show that the 60-minute RTI model failed to predict landslides in three of the study areas, while both the 30- and 10-minute RTI models gave successful predictions for all of the study areas. Each RTI model showed different mean response times to landslide warning: 4.04 hours in the 60-minute RTI model, 6.08 hours in the 30-minute RTI model, and 9.15 hours in the 10-minute RTI model. Longer response times to landslides were possible using models that considered rainfall intensity for shorter periods of time. Considering the large variations in rainfall intensity that may occur within short periods in Korea, it is possible to increase the accuracy of prediction, and thereby improve the early warning of landslides, using a RTI model that considers rainfall intensity for periods of less than 1 hour.

• The Korean Journal of Food And Nutrition
• /
• v.27 no.3
• /
• pp.532-537
• /
• 2014

Cortex Phellodendri (CP) is derived from the dried bark of Phellodendron amurense. It has been widely used as a drug in traditional Korea medicine for treating diarrhea, jaundice, swelling pains in the knees and feet, urinary tract infections, and infections of the body surface. Many analytical methods have been used to study oriental herbal medicines, such as thin-layer chromatography, column liquid chromatography, and high performance liquid chromatography (HPLC). In this study, preparative centrifugal partition chromatography (CPC) was successfully carried out in order to separate pure compounds from a CP methanol extract. The optimum two-phase CPC solvent system was composed of n-butanol: acetic acid: water (4:1:5 v/v/v). The flow rate of the mobile phase was 3 mL/min in ascending mode with rotation at 1,000 rpm. The CPC-separated fraction and purification procedures were carried out by preparatory HPLC. The $^1H$ NMR spectrum revealed that the resonances at ${\delta}$ 4.10 and 4.20 ppm corresponded to three protons ($-OCH_3$), whereas those at ${\delta}$ 6.10 ppm corresponded to two protons ($-OCH_2O-$). Further, two aromatic protons (H-11 and H-12) conveys a doublet-doublet pattern. The H-11 doublet and H-12 doublet appear at ${\delta}$ 7.98 and 8.11, respectively. The $^{13}C$ NMR. spectrum showed a tetrasubstituted with a methylenedioxy group at C2 and C3, and two methoxy groups at C9 and C10. The chemical structure of the berberine was identified by $^1H$, $^{13}C$-nuclear magnetic resonance and electrospray ionization-mass spectroscopy spectral data analysis.

• Journal of Biomedical Engineering Research
• /
• v.25 no.5
• /
• pp.323-328
• /
• 2004

The development of group-specific tissue probability maps (TPM) provides a priori knowledge for better result of cerebral tissue classification with regard to the inter-ethnic differences of inter-subject variability. We present sequential procedures of group-specific TPM and evaluate the age effects in the structural differences of TPM. We investigated 100 healthy volunteers with high resolution MRI scalming. The subjects were classified into young (60, 25.92+4.58) and old groups (40, 58.83${\pm}$8.10) according to the age. To avoid any bias from random selected single subject and improve registration robustness, average atlas as target for TPM was constructed from skull-stripped whole data using linear and nonlinear registration of AIR. Each subject was segmented into binary images of gray matter, white matter, and cerebrospinal fluid using fuzzy clustering and normalized into the space of average atlas. The probability images were the means of these binary images, and contained values in the range of zero to one. A TPM of a given tissue is a spatial probability distribution representing a certain subject population. In the spatial distribution of tissue probability according to the threshold of probability, the old group exhibited enlarged ventricles and overall GM atrophy as age-specific changes, compared to the young group. Our results are generally consistent with the few published studies on age differences in the brain morphology. The more similar the morphology of the subject is to the average of the population represented by the TPM, the better the entire classification procedure should work. Therefore, we suggest that group-specific TPM should be used as a priori information for the cerebral tissue classification.

• Smart Media Journal
• /
• v.7 no.1
• /
• pp.37-44
• /
• 2018

Utilizing sequence control and numerical computing, embedded devices are used in a variety of automated systems, including those at industrial sites, in accordance with their control program. Since embedded devices are used as a control system in corporate industrial complexes, nuclear power plants and public transport infrastructure nowadays, deliberate attacks on them can cause significant economic and social damages. Most attacks aimed at embedded devices are data-coded, code-modulated, and control-programmed. The control programs for industry-automated embedded devices are designed to represent circuit structures, unlike common programming languages, and most industrial automation control programs are designed with a graphical language, LAD, which is difficult to process static analysis. Because of these characteristics, the vulnerability analysis and security related studies for industry automation control programs have only progressed up to the formal verification, real-time monitoring levels. Furthermore, the static analysis of industrial automation control programs, which can detect vulnerabilities in advance and prepare for attacks, stays poorly researched. Therefore, this study suggests a method to present a discussion on an industry automation control program designed to represent the circuit structure to increase the efficiency of static analysis of embedded industrial automation programs. It also proposes a medium term translation technology exploiting LLVM IR to comprehensively analyze the industrial automation control programs of various manufacturers. By using LLVM IR, it is possible to perform integrated analysis on dynamic analysis. In this study, a prototype program that converts to a logical expression type of medium language was developed with regards to the S company's control program in order to verify our method.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.35 no.3
• /
• pp.153-157
• /
• 2009

Recently, an increasing number of bisphosphonate related osteonecrosis of the jaw(BRONJ) is being reported. A guideline has been already established in the US, but it does not seem to be fully recognized by clinicians in Korea. Therefore, a survey study was done to inform and have clinicians realize the seriousness of BRONJ. 1,341 practitioners were randomly selected out of 13,405 practitioners(by Feb of 2008, KDA) in Korea. A questionnaire was given to them between May to July in 2008. Questions were designed to investigate each respondent's experience term years in the clinic, occupation, speciality, awareness on risk of bisphosphonate, experience on treating osteonecrosis patients, awareness about the guideline on BRONJ suggested by AAOMS and whether if they ask about bisphosphonate medication history to patients before invasive treatment. 45.1% of the clinicians have reported on experiencing delayed healing on bone exposed site after extraction both in the maxilla and the mandible. However, clinicians have asked the patients whether if they are on bisphosphonate or not in only 15.1% of these cases. 56.5% of the clinicians simply knew about BRONJ but only 28.9% of the clinicians were aware that bisphosphonate can cause osteonecrosis after invasive dental treatment. Only 19.3% knew about the contents of guideline on BRONJ and 57.2% were aware of the seriousness of BRONJ. Clinicians with shorter clinical experience term were more aware of BRONJ and the guideline on BRONJ than the experienced clinicians. But awareness of the possibility of BRONJ after invasive dental treatment were about the same regardless of their clinical experience. The results show that Korean clinicians need to be more aware about BRONJ. Data on BRONJ cases in Korea should be collected and provided with additional education to let Korean clinicians know and be more aware about BRONJ.

• Tuberculosis and Respiratory Diseases
• /
• v.59 no.5
• /
• pp.480-486
• /
• 2005

Since the year 2000, lung cancer has become the leading cause of cancer death in South Korea as in many other parts of the world. The current multidisciplinary approach for lung cancer includes a wide range of modalities, not only surgery, radiotherapy, medical drug therapy but also pain control, as well as social and psychological support. Therefore, thoracic surgeons, radiologists, nuclear medicine specialists, anesthetists, psychologist, nurses and social workers as well as medical doctors care for lung cancer patients. Sharing a common treatment protocol and optimal communication are vital aspects of shared care both from a medical and cost-effectiveness point of view. We developed a shared electronic medical record (SEMR) for treating patients with lung cancer in a university hospital to facilitate the sharing protocols and communications between doctors involved in a lung cancer clinic. A SEMR system was developed within a order communication system(OCS) for a lung cancer clinic. The records of radiological, laboratory and pathological studies as well as the records of surgery, chemotherapy, and radiotherapy were stored and presented to all doctors who treat the same patient. Every doctor was allowed to change his/her own records. They could review other doctor s records but could not alter them. With the SEMR, it was expected that the time to complete the medical records for one patient could be reduced because it was easy to review all the data from the other doctors who share the same patient. In addition, the confidence of the doctors who share a common treatment protocol would be higher. Therefore, a shared electronic medical record is expected to improve the quality of patient care.

• The Korean Journal of Mycology
• /
• v.37 no.1
• /
• pp.11-18
• /
• 2009

Phylogenetic relationships were investigated in the mushroom genus Agrocybe based on sequence data from the internal transcribed spaces(ITS) of nuclear ribosomal DNA(rDNA). Thirty strains including Agrocybe aegerita, A. grocybe cylindracea, A. grocybe praecox, A. grocybe pediades and Unknown Agrocybe species were subjected for the analysis. The Agrocybe spp. were separated into eight distinct groups. Phylogenetic analysis divided these species into eight groups as follows: A. chaxingu within group I, A. salicacola within group II, A. cylindracea and A. aegerita within group III, Agrocybe sp. within group IV and V, and other Agrocybe spp. were grouped within VI, VII, and VIII.

• Journal of Ginseng Research
• /
• v.41 no.2
• /
• pp.134-143
• /
• 2017

Background: The prevalence of allergic inflammatory diseases such as atopic dermatitis (AD), asthma, and allergic rhinitis worldwide has increased and complete recovery is difficult. Korean Red Ginseng, which is the heat-processed root of Panax ginseng Meyer, is widely and frequently used as a traditional medicine in East Asia. In this study, we investigated whether Korean Red Ginseng water extract (RGE) regulates the expression of proinflammatory cytokines and chemokines via the mitogen-activated protein kinases (MAPKs)/nuclear factor kappa B ($NF-{\kappa}B$) pathway in allergic inflammation. Methods: Compound 48/80-induced anaphylactic shock and 1-fluoro-2,4-dinitrobenzene (DNFB)-induced AD-like skin lesion mice models were used to investigate the antiallergic effects of RGE. Human keratinocytes (HaCaT cells) and human mast cells (HMC-1) were also used to clarify the effects of RGE on the expression of proinflammatory cytokines and chemokines. Results: Anaphylactic shock and DNFB-induced AD-like skin lesions were attenuated by RGE administration through reduction of serum immunoglobulin E (IgE) and interleukin (IL)-6 levels in mouse models. RGE also reduced the production of proinflammatory cytokines including $IL-1{\beta}$, IL-6, and IL-8, and expression of chemokines such as IL-8, thymus and activation-regulated chemokine (TARC), and macrophage-derived chemokine (MDC) in HaCaT cells. Additionally, RGE decreased the release of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), $IL-1{\beta}$, IL-6, and IL-8 as well as expressions of chemokines including macro-phage inflammatory protein $(MIP)-1{\alpha}$, $MIP-1{\beta}$, regulated on activation, normal T cell expressed and secreted (RANTES), monocyte chemoattractant protein (MCP)-1, and IL-8 in HMC-1 cells. Furthermore, our data demonstrated that these inhibitory effects occurred through blockage of the MAPK and $NF-{\kappa}B$ pathway. Conclusion: RGE may be a useful therapeutic agent for the treatment of allergic inflammatory diseases such as AD-like dermatitis.

• Solar Energy
• /
• v.15 no.3
• /
• pp.3-14
• /
• 1995

The Direct Contact heat Exchanger(DCHX) has been widely studied in the chemical industry for many years due to its inherent simplicity as a counter-current divice for heat and mass transfer. In many solar systems, the DCHX unit can be combined with the thermal storage unit, or alternatively, it can be used separately from the storage unit, much like an external(to storage) closed heat exchanger system. In the present work, the spray column type of direct contact heat exchangers are studied extensively to harness the solar energy for hot water and spaced heating. Some of the major considerations that are involved in the design of heat exchangers in this study are that : working fluid is a hydrocaabon(such as Texaterm) or water which is either lighter or heavier than storage medium. The experimental data have revealed some interesting characteristics concerning the application of DCHXs for solar energy utilization. These experiments are carried out in the line of solar heating system, major results are as follows : 1) the flow and aspect of working fluid drop for maxium heat transfer 2) efficiency and volumetric heat transfer coefficient of D.C.H.X with a heavier working fluid are higher than those of D.C.H.X with a lighter working fluid.