• BMB Reports
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• 제37권4호
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• pp.474-479
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• 2004

The expressed sequence tag (EST) effort in Toxoplasma gondii has generated a substantial amount of gene information. To exploit this valuable resource, we chose to study tgd057, a novel gene identified by a large number of ESTs that otherwise show no significant match to known sequences in the database. Northern analysis showed that tgd057 is transcribed in this tachyzoite. The complete cDNA sequence of tgd057 is 1169 bp in length. Sequence analysis revealed that tgd057 possibly adopts two polyadenylation sites, utilizes the fourth in-frame ATG for translation initiation, and codes for a secretory protein. The longest open reading frame for the tgd057 gene was cloned and expressed as a recombinant protein (rd57) in Escherichia coli. Western analysis revealed that serum against rd57 recognized a molecule of ~21 kDa in the tachyzoite protein extract. This suggests that the tgd057 gene is expressed in vivo in the parasite.

• Steel and Composite Structures
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• 제18권5호
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• pp.1197-1214
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• 2015

There is a great difference in mechanical behavior between design model one-time loading and step-by-step construction process. This paper presents practical computational methods for simulating the structural behavior of long-span rigid steel structures during construction processes. It introduces the positioning principle of node rectification for installation which is especially suitable for rigid long-span steel structures. Novel improved nonlinear analytical methods, known as element birth and death of node rectification, are introduced based on several calculating methods, as well as a forward iteration of node rectification method. These methods proposed in this paper can solve the problem of element's 'floating' and can be easily incorporated in commercial finite element software. These proposed methods were eventually implemented in the computer simulation and analysis of the main stadium for the Universiade Sports Center during the construction process. The optimum construction scheme of the structure is determined by the improved algorithm and the computational results matched well with the measured values in the project, thus indicating that the novel nonlinear time-varying analysis approach is effective construction simulation of complex rigid long-span steel structures and provides useful reference for future design and construction.

• Steel and Composite Structures
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• 제23권4호
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• pp.435-444
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• 2017

In the past, the progressive collapse resulting from local failures during accidents has caused many tragedies and loss of life. Although long-span spatial grid structures are characterised by a high degree of static indeterminacy, the sudden failure of key members may lead to a catastrophic progressive collapse. For this reason, it is especially necessary to research the progressive collapse resistance capacity of long-span spatial grid structures. This paper presents an evaluation method of important members and a novel dynamic analysis method for simulating the progressive collapse of long-span spatial grid structures. Engineering cases were analysed to validate these proposed method. These proposed methods were eventually implemented in the progressive collapse analysis of the main stadium for the Universiade Sports Center. The roof of the structure was concluded to have good resistance against progressive collapse. The novel methods provide results close to practice and are especially suitable for the progressive collapse analysis of long-span spatial grid structures.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.621-629
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• 2021

Shigella flexneri is a facultative intracellular pathogen that causes bacillary dysentery in humans. Infection with S. flexneri can result in more than a million deaths yearly and most of the victims are children in developing countries. Therefore, identifying novel and unique drug targets against this pathogen is instrumental to overcome the problem of drug resistance to the antibiotics given to patients as the current therapy. In this study, a comparative analysis of the metabolic pathways of the host and pathogen was performed to identify this pathogen's essential enzymes for the survival and propose potential drug targets. First, we extracted the metabolic pathways of the host, Homo sapiens, and pathogen, S. flexneri, from the KEGG database. Next, we manually compared the pathways to categorize those that were exclusive to the pathogen. Further, all enzymes for the 26 unique pathways were extracted and submitted to the Geptop tool to identify essential enzymes for further screening in determining the feasibility of the therapeutic targets that were predicted and analyzed using PPI network analysis, subcellular localization, druggability testing, gene ontology and epitope mapping. Using these various criteria, we narrowed it down to prioritize 5 novel drug targets against S. flexneri and one vaccine drug targets against all strains of Shigella. Hence, we suggest the identified enzymes as the best putative drug targets for the effective treatment of S. flexneri.

• Journal of Periodontal and Implant Science
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• 제42권2호
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• pp.59-63
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• 2012

Purpose: This study evaluated the surface characteristics and bond strength produced using a novel technique for coating hydroxyapatite (HA) onto titanium implants. Methods: HA was coated on the titanium implant surface using a super-high-speed (SHS) blasting method with highly purified HA. The coating was performed at a low temperature, unlike conventional HA coating methods. Coating thickness was measured. The novel HA-coated disc was fabricated. X-ray diffraction analysis was performed directly on the disc to evaluate crystallinity. Four novel HA-coated discs and four resorbable blast medium (RBM) discs were prepared. Their surface roughnesses and areas were measured. Five puretitanium, RBM-treated, and novel HA-coated discs were prepared. Contact angle was measured. Two-way analysis of variance and the post-hoc Scheffe's test were used to analyze differences between the groups, with those with a probability of P<0.05 considered to be statistically significant. To evaluate exfoliation of the coating layer, 7 sites on the mandibles from 7 mongrel dogs were used. Other sites were used for another research project. In total, seven novel HA-coated implants were placed 2 months after extraction of premolars according to the manufacturer's instructions. The dogs were sacrificed 8 weeks after implant surgery. Implants were removed using a ratchet driver. The surface of the retrieved implants was evaluated microscopically. Results: A uniform HA coating layer was formed on the titanium implants with no deformation of the RBM titanium surface microtexture when an SHS blasting method was used. Conclusions: These HA-coated implants exhibited increased roughness, crystallinity, and wettability when compared with RBM implants.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 춘계학술대회 논문집
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• pp.1319-1320
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• 2010

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.72-72
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• 2001

• 한국광학회:학술대회논문집
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• 한국광학회 2006년도 하계학술발표회 논문집
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• pp.43-44
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• 2006

• Journal of Electrical Engineering and Technology
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• 제1권2호
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• pp.185-191
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• 2006

In this paper, a novel stator hybrid excited doubly salient permanent magnet (SHEDS-PM) brushless machine with a special magnetic bridge is proposed for the first time. The originality of this machine is purposely to add a magnetic bridge in shunt with each PM pole, which not only maintains the stator lamination in its entireness, but also amplifies the effect of DC field flux on PM flux. An equivalent magnetic circuit is presented to clarify the novelty. Based on the 2-D finite element analysis, the static characteristics of the SHEDS-PM machine, namely phase flux linkage, back-EMF, cogging torque, winding inductance and static torque are deduced. The corresponding results on a prototype machine illustrate that the proposed machine is promising for application to electric vehicles.

• Mycobiology
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• 제42권1호
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• pp.12-16
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• 2014

We isolated and examined a new Alternaria sp., which causes leaf spots on Peucedanum japonicum in Korea, by using molecular and morphological methods. Phylogenetic analysis based on a combined internal transcribed spacer region analysis and two protein-coding genes (gpd and Alt a1) demonstrated that the causal fungus was most closely related to A. cinerariae and A. sonchi, and relevant to A. brassicae. However, conidial morphology indicated that it is a novel species within the genus Alternaria, and therefore we have assigned the fungus a new name in this study.