• Progress in Medical Physics
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• v.7 no.2
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• pp.29-40
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• 1996

We had designed and made the cylindrical ionization chamber which operated above 5 mR/h. Using commercial electrometer, we investigated the characterictic of charge collection in the ion chamber. The active volume was 190.4㎤ and overall length and diameter in the chamber was 15.5cm, 5.22cm, respectively. The chamber had three electrodes(inner, central, wall electrode). And background current was 8.39${\times}$10$\^$-14/${\pm}$1.5${\times}$10$\^$-15/A to arrange the electrodes which were coaxial in chamber axis. The collection efficiency of chamber for Cs$\^$137/ was 99.7％ when the opreating voltage was applied 400V. Comparing with the commertial dosimetry system, the exposure calibration constant was 4.531${\times}$19$\^$7/R/C. By normalizing to CS$\_$137/ the relative energy response of the chamber was 1.30 for Am$\_$24/, 1.05 for C0$\_$60/, respectively. When the irrarition tranversed to the chamber axis, the isotropic effect of the chamber was not considerable.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.8
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• pp.751-757
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• 2016

The aim of this study is to estimate the reliability of fatigue limit of the material used for crank throw components according to the staircase method. The material used for crank throw components is forged S34MnV grade steel, which is heat treated by normalizing and tempering. In this work, to predict the reliability of the design fatigue strength, axially loaded constant amplitude fatigue testing was conducted. The test specimens were loaded with an axial push/pull load with a mean stress of 0 MPa, which corresponds to a stress ratio of R=-1. The fatigue test results were evaluated by Dixon-Mood formulas. The values of mean fatigue strength and standard deviation predicted by the staircase method were 296.3 MPa and 10.6 MPa, respectively. Finally, the reliability of the fatigue limit in some selected probability of failure is predicted. The proposed method can be applied for the determination of fatigue strength for design optimization of the forged steel.

• Transactions of the Korean Society of Mechanical Engineers
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• v.13 no.2
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• pp.307-315
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• 1989

In recent years, the fatigue design method by analysis for the mechanical components and the welded structures has much increased, instead of the fatigue design method by rule that has been widely used from the past days. When a fatigue design is conducted by that method, the basic informations, fatigue life curves are mainly obtained from the results of the strain controlled low cycle fatigue test. From these point of views, the low cycle fatigue test is coming to be given a much importance lately. In this paper, the strain controlled low cycle fatigue properties at room temperature in air environment were investigated for the low carbon forged steel, SF45A, and the rolled steel for the welded structure, SM 41B. Throughout the test, strain ratio, R, was maintained constant with the fully reversed condition, -1. As the experimental results, the cyclic stress-strain behaviours of the test materials were different each other, but the low cycle fatigue life-time of them appeared to show little difference in the region of this test conditions.

• Journal of Animal Science and Technology
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• v.57 no.5
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• pp.18.1-18.8
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• 2015

Background: Quantitative real time reverse transcription PCR (qRT-PCR) is one of the most important techniques for gene-expression analysis in molecular based studies. Selecting a proper internal control gene for normalizing data is a crucial step in gene expression analysis via this method. The expression levels of reference genes should be remained constant among cells in different tissues. However, it seems that the location of cells in different tissues might influence their expression. The purpose of this study was to determine whether the source of mesenchymal stem cells (MSCs) has any effect on expression level of three common reference genes (GAPDH, ${\beta}$-actin and ${\beta}2$-microglobulin) in equine marrow- and adipose-derived undifferentiated MSCs and consequently their reliability for comparative qRT-PCR. Materials and methods: Adipose tissue (AT) and bone marrow (BM) samples were harvested from 3 mares. MSCs were isolated and cultured until passage 3 (P3). Total RNA of P3 cells was extracted for cDNA synthesis. The generated cDNAs were analyzed by quantitative real-time PCR. The PCR reactions were ended with a melting curve analysis to verify the specificity of amplicon. Results: The expression levels of GAPDH were significantly different between AT- and BM-derived MSCs (p < 0.05). Differences in expression level of ${\beta}$-actin (P < 0.001) and B2M (P < 0.006.) between MSCs derived from AT and BM were substantially higher than GAPDH. In addition, the fold change in expression levels of GAPDH, ${\beta}$-actin and B2M in AT-derived MSCs compared to BM-derived MSCs were 2.38, 6.76 and 7.76, respectively. Conclusion: This study demonstrated that GAPDH and especially ${\beta}$-actin and B2M express in different levels in equine AT- and BM-derived MSCs. Thus they cannot be considered as reliable reference genes for comparative quantitative gene expression analysis in MSCs derived from equine bone marrow and adipose tissue.