• Animal Bioscience
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• v.34 no.4
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• pp.575-583
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• 2021

Objective: The aim of the study was to determine the effect of parity and the stage of lactation on the metabolic profile of cows based on the basic chemical milk components and the blood parameters. Methods: The study material consisted of high-yielding Holstein-Friesian cows. In total, 473 cows were examined. According to the parity, cows were divided into four groups: primiparous (P), and multiparous in the second (M2), in the third (M3), and in subsequent lactations (M4). The feeding of cows was based on total mixed ration (TMR) ad libitum. Milk and blood samples were collected individually from each cow three times per standard lactation period. Results: Greater exacerbation of changes in the dynamics of the blood plasma parameters examined was proved for multiparous cows. The highest value of β-hydroxybutyrate acid (0.946 mmol/L) was found for multiparous cows from group M3 at the beginning of lactation. However, it was still in the normal range. The results showed aspartate aminotransferase, and gamma-glutamyl transferase (GGT) activities in dairy cows during lactation had significant variations taking in to account stage of lactation. The highest activity of GGT was found in the group of the oldest cows and measured from 26.36 U/L at the beginning of lactation to 48.75 U/L at the end of the lactation period. Conclusion: The time-related changes in the concentrations of the biochemical parameters described differ markedly among lactating cows, though the housing conditions on the research dairy farm are highly standardised. This indicates that the ability to cope with metabolic stress is mainly affected by the individual predispositions of cows and feed nutrient supply in different stage of lactation. Especially, the feed nutrient supply (in net energy for lactation), which was the best in TMR 1 in comparison TMR 3.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.21 no.4
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• pp.143-148
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• 2021

A systematic study of Bloch surface wave (BSW), which is created by guided mode resonance (GMR) of dielectric multilayer structures with a grating profile, is presented to analyze the sensing performance of bio-sensors. The effect of structural parameters on optical behavior is evaluated by using Babinet's principle and modal transmission-line theory. The sensitivity of designed bio-sensors is proportional to the grating constant at wavelength spectrum, and inversely proportional to the normal wave vector of incident electromagnetic wave at angular spectrum. Numerical results for two devices with SiO/SiO₂ and TiO₂/SiO₂ multilayer dielectric stacks are presented, showing that BSW can be exploited for the realization of efficient diffraction-based bio-sensors from infrared to visible-band range.

• Geomechanics and Engineering
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• v.33 no.5
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• pp.477-486
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• 2023

In the studies on fault dislocation of tunnel, existing literatures are mainly focused on the problems caused by normal and reverse faults, but few on strike-slip faults. The paper aims to research the deformation and failure mechanism of a tunnel under strike-slip faulting based on a model test and test-calibrated numerical simulation. A potential faulting hazard condition is considered for a real water tunnel in central Yunnan, China. Based on the faulting hazard to tunnel, laboratory model tests were conducted with a test apparatus that specially designed for strike-slip faults. Then, to verify the results obtained from the model test, a finite element model was built. By comparison, the numerical results agree with tested ones well. The results indicated that most of the shear deformation and damage would appear within fault fracture zone. The tunnel exhibited a horizontal S-shaped deformation profile under strike-slip faulting. The side walls of the tunnel mainly experience tension and compression strain state, while the roof and floor of the tunnel would be in a shear state. Circular cracks on tunnel near fault fracture zone were more significant owing to shear effects of strike-slip faulting, while the longitudinal cracks occurred at the hanging wall.

• IMMUNE NETWORK
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• v.22 no.6
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• pp.50.1-50.19
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• 2022

Autoreactive B cells are not entirely deleted, but some remain as immunocompetent or anergic B cells. Although the persistence of autoreactive B cells as anergic cells has been shown in transgenic mouse models with the expression of B cell receptor (BCR) reactive to engineered self-antigen, the characterization of naturally occurring anergic B cells is important to identify them and understand their contribution to immune regulation or autoimmune diseases. We report here that a low-level expression of CD138 in the splenic B cells marks naturally arising anergic B cells, not plasma cells. The CD138^int B cells consisted of IgM^lowIgD^high follicular (FO) B cells and transitional 3 B cells in homeostatic conditions. The CD138^int FO B cells showed an anergic gene expression profile shared with that of monoclonal anergic B cells expressing engineered BCRs and the gene expression profile was different from those of plasma cells, age-associated B cells, or germinal center B cells. The anergic state of the CD138^int FO B cells was confirmed by attenuated Ca²⁺ response and failure to upregulate CD69 upon BCR engagement with anti-IgM, anti-IgD, anti-Igκ, or anti-IgG. The BCR repertoire of the CD138^int FO B cells was distinct from that of the CD138^- FO B cells and included some class-switched B cells with low-level somatic mutations. These findings demonstrate the presence of polyclonal anergic B cells in the normal mice that are characterized by low-level expression of CD138, IgM downregulation, reduced Ca²⁺ and CD69 responses upon BCR engagement, and distinct BCR repertoire.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.22 no.2
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• pp.206-216
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• 2000

Predictional study for lateral change between pre- and post-orthognathic surgery has been emphasized mainly on anterior area of lateral profile; upper lip, lower lip and chin et al. So interest for lateral profile change has been less in posterior area of lateral profile and literature analyzing gonial angle change is rare. The purpose of this study is to make prediction for gonial angle change possible and to offer somewhat treatment guidance for gonial angle to be improved by investigating overall gonial angle change between pre- and post-orthognathic surgery and inquiring into factors influencing on pattern of genial angle change. For this study 35 patients were selected retrospectively. Lateral cephalometric radiographs were taken in just pre-op time, pod 1 day, pod 1 year. They were analyzed and genial angles were measured. The results were as follows : 1. Gonial angle at pod 1 day was decreased about $9.3^{\circ}$ than pre-op and gonial angle at pod 1 year was increased about $4.0^{\circ}$ than pod 1 day. So genial angle at pod 1 year was decreased about $5.3^{\circ}$ than pre-op genial angle(p<0.01). 2. Mean pre-op gonial angle was $129.4^{\circ}$, showing significantly high value than normal and mean gonial angle at pod 1 year was $124.1^{\circ}$, showing value near to normal. 3. Mean gonial angle change between pre-op and pod 1 year was decreased about $5.4^{\circ}$ in female and $5.3^{\circ}$ in male. There was no statistically significant difference between male and female(p>0.05). 4. Principal factor influencing on decreased gonial angle in gonial angle change between pre-op and pod 1 year was amount of mandibular setback. 5. Principal factor influencing on increased gonial angle in gonial angle change between pod 1 day and pod 1 year was % horizontal relapse, and it was thought that resorption and bone remodelling on posterior area in mandibular distal segment also were related to increased gonial angle. 6. It is thought that sagittal split ramus osteotomy in mandibular prognathic patients with high value of gonial angle is effective to improvement of gonial angle, and In patients who have normal range of gonial angle and are required with excessive mandibular setback, short lingual cut method, additional resection of posterior margin of distal segment, Obwegeser II method will be considerd. 7. More prudent operation and careful post-op management will be responsible for maintenance of postoperative stable gonial angle.

• The korean journal of orthodontics
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• v.27 no.1
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• pp.1-20
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• 1997

Skeletal Class III malocclusions are growth-related discrepancies, and the problems are more severe until growth is complete. Causes of skeletal Class III malocclusion are classified into mandibular overgrowth, maxillary deficiency, and combination of the two. Face mask has been recommended for treatment of Class III malocclusion with maxillary deficiency in the early time of growth. Numerous experiments were performed and clinical studies have been reported on face mask ; nevertheless, studies on profile changes and stability after treatment of face mask are considered to be somewhat insufficient. The author selected 50 patients who can be checked for follow-up. They had been diagnosed as skeletal Class III malocclusion with maxillary deficiency and then treated with face mask ; the sample group was divided according to sex, treatment beginning age, palatal suture opening (intraoral appliance). For each group, changing pattern of facial profile and stability of treatment observed, and comparison with 20 Korean normal children(Angle's Class I). The following results were obtained. 1. skeletal, dental, and soft tissue measurements indicated more changes in the amounts of maxillary forward movement during face mask treatment. 2. R.P.E. group showed more significant maxillofacial changes and La-Li group showed more dental changes. 3. Growth changes of maxilla induced in the treatment group during wearing face mask were much more than those of normal group. 4. Growth changes of maxilla in the treatment group after treatment of face mask were less than those of normal group. From the obtained aata, it can be concluded that there was a stimulative effect on forward growth of maxilla during the use of face mask ; however, on removal of face mask, the stimulative effect was eliminated and undergrowth tendency of maxilla resumed.

• Journal of Oral Medicine and Pain
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• v.35 no.2
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• pp.135-147
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• 2010

In this study, the objective masticatory efficiency of two groups of temporomandibular disorder patients, pain and sound groups, was compared with that in a normal group using the MAI (mixing ability Index). The subjective chewing ability was evaluated using questionnaires, such as the Food Intake Ability Index (FIA) and Visual Analogue Scale (VAS). The Oral Health Impact Profile (OHIP)-49K of the patients was also examined to measure the oral health-related quality of life. The results were as follows: 1. The MAI, FIA and VAS in the pain group were significantly lower than in the normal and sound groups. This shows that the chewing efficiency of the pain group was lower than the normal and sound groups (P<0.05). However, there was no significant difference between the sound and normal groups. 2. The OHIP-49K for the oral health-related quality of life showed a significant increase in both the pain and sound groups compared with normal group. This means that the oral health-related quality of life was lower in both the pain and sound groups. 3. There was a correlation between the MAI, FIA and VAS (P<0.01) in all subjects (71 persons). The OHIP-49K was associated with the FIA and VAS. 4. There was a correlation between the FIA and VAS (P<0.05) in the sound group but no correlation in the other groups. 5. There was a correlation between the FIA and VAS in all groups. 6. The VAS was increased significantly in the pain group according to the level of pain reduction after treatment (P<0.05). However, there was no significant increase in the MAI, even though there was an improvement in masticatory efficiency. In addition, there was no difference in the FIA and OHIP-49K according to the level of pain reduction after treatment. In this study, it is believed that pain is a main factor decreasing the masticatory efficiency in patients with temporomandibular disorders. Moreover, TMJ sounds decrease the quality of life but do not decrease the masticatory efficiency. Therefore, it is important to control the pain in order to improve the masticatory efficiency in temporomandibular disorder patients. Moreover, managing both pain and sound can improve the quality of life.

• Journal of Biomedical Engineering Research
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• v.22 no.5
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• pp.449-458
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• 2001

This is a test report of system efficiency for the moving-actuator type Bi-ventricular assist device (AnyHear $t^{MT}$ ) Seoul National University). $AnyHeart^{TM}$), as an energy converter. utilities a brushless DC motor(S/M 566-26A. Sierracin/ Magnedyne, Carlsbad, CA. U.S.A.) generating their pendulous motion in the epicyclic gear train. It is necessary to know about the overall efficiency of the system. The system is subdivided into three parts: motor part, actuator part and blood sac part (including valves, etc.) according to system mechanism. The motor was operated with a variable range of torque. angular speed and width of voltage Pulse In this report. $AnyHeart^{TM}$ is focused on the efficiency of the motor and actuator parts. 4 $\ell/min$ pump output. which is normal condition of $AnyHeart^{TM}$ system, the total system efficiency is 8%, which is composed of 50%, 85% and 17% efficiency (motor Part, actuator Part and blood sac Part) respectively. In the analyzed result. applied input voltage on normal condition of $AnyHeart^{TM}$ is determined. Also speed Profile with considering filling state of blood sac is Provided. In the test of the in vitro mock circulation. some experimental results are Provided to demonstrate the effectiveness of the Presented approach.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.15 no.1
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• pp.75-81
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• 2004

Objectives：This study was designed to compare the emotional/behavioral characteristics between the siblings of children with autism and normally developing children. Methods：The subjects were 34 children between 10 and 16 years of age, 17 siblings of children with autism, 17 siblings of nonhandicapped children. Both groups performed CDI(Kovacs' Children Depression Inventory), RCMAS(Revised Chidren's Manifest Anxiety Scale), SPPC(Self-Perception Profile for Children), Self-esteem scale. Their mothers performed K-CBCL(Korean -Child Behavior Checklist). The results of those test were analysed by Student's-test. Results：Both social competence and total social competence subsclae scores were significantly lower in sibling group of children with autism than in control group. However, each group was not significantly different on the scores of internalizing symptom, externalizing symptom subscale of CBCL, CDI, RCMAS, Self-Perception Profile and Self-esteem scale. Conclusion : Siblings of children with autism had a lower social competence than normal control group. Group comparisons indicated that siblings of children with autism and normal children did not differ on any self-report measures such as anxiety, depression, self-concept and self-esteem. Further research using more subjects will be needed.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.1499-1506
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• 2016

Background: Crocus sativus and its major constituent crocin are well established to have anti-cancer properties in breast cancer cells (MCF-7). However the role of C. sativus extract (CSE) and crocin on caspase signaling mediated MCF-7 cell death at molecular level is remains unclear. In this study, we tried to unravel role of CSE and crocin on caspase mediated MCF-7 cells death and their in vivo preclinical toxicity profiling and immune stimulatory effect. Materials and Methods: CSE extract was fractionated by HPLC and crocin was isolated and characterized by NMR, IR, and MS. MCF-7 cells were treated with both CSE and crocin and expression of Bcl-2 and Bax was assessed after 24 and 36 hours. Furthermore, caspase 3, caspase 8 and caspase 9 expression was determined by Western blotting after 24 hours of treatment. DNA fragmentation analysis was performed for genotoxicity of CSE and crocin in MCF-7 cells. The in vivo toxicity profile of CSE (300 mg/kg of b.wt) was investigated in normal Swiss albino mice. In addition, peritoneal macrophages were collected from crocin (1, 1.5 and 2 mg/kg body weight) treated mice and analyzed for ex vivo yeast phagocytosis. Results: Immunoblot analysis revealed that there was time dependent decline in anti-apoptotic Bcl-2 with simultaneous upregulation of Bax in CSE and crocin treated MCF-7 cells. Further CSE and crocin treatment downregulated caspase 8 and 9 and cleaved the caspase 3 after 24 hours. Both CSE and crocin elicited considerable DNA damage in MCF-7 cells at each concentration tested. In vivo toxicity profile by histological studies revealed no observable histopathologic differences in the liver, kidney, spleen, lungs and heart in CSE treated and untreated groups. Crocin treatment elicited significant dose and time dependent ex vivo yeast phagocytosis by peritoneal macrophages. Conclusions: Our study delineated involvement of pro-apoptotic and caspase mediated MCF-7 cell death by CSE and crocin at the molecular level accompanied with extensive DNA damage. Further we found that normal swiss albino mice can tolerate the maximum dose of CSE. Crocin enhanced ex vivo macrophage yeast phagocytic ability.