• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3751-3755
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• 2013

Backgroud and Aims: MicroRNA-206 has proven to be down-regulated in many human malignancies in correlation with tumour progression. Our study aimed to characterize miR-206 contributions to initiation and malignant progression of human osteosarcoma. Methods: MiR-206 expression was detected in human osteosarcoma cell 1ine MG63, human normal osteoblastic cell line hFOB 1.19, and paired osteosarcoma and normal adjacent tissues from 65 patients using quantitative RT-PCR. Relationships of miR-206 levels to clinicopathological characteristics were also investigated. Moreover, miR-206 mimics and negative control siRNA were transfected into MG63 cells to observe effects on cell viability, apoptosis, invasion and migration. Results: We found that miR-206 was down-regulated in the osteosarcoma cell line MG63 and primary tumor samples, and decreased miR-206 expression was significantly associated with advanced clinical stage, T classification, metastasis and poor histological differentiation. Additionally, transfection of miR-206 mimics could reduce MG-63 cell viability, promote cell apoptosis, and inhibit cell invasion and migration. Conclusions: These findings indicate that miR-206 may have a key role in osteosarcoma pathogenesis and development. It could serve as a useful biomarker for prediction of osteosarcoma progression, and provide a potential target for gene therapy.

• Journal of Periodontal and Implant Science
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• 제36권4호
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• pp.817-827
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• 2006

Purpose : This study was designed 1) to compare the perception of dental professionals and lay people with respect to minor variations in maxillary anterior tooth size and alignment and their relation to the surrounding soft tissues, and 2) to evaluate the normal tooth-gingiva topographical relationships in periodontally healthy young subjects, Materials and methods : Maxillary anterior teeth were intentionally diagrammed in varying degree of deviation with respect to one of three common anterior esthetic discrepancies including variations in crown length, shape of gingival margin, and length of interproximal contact, 17 images were generated to be preferentially selected by 2 groups consisting of dental professionals and lay people (total of 740). Smiling photographs of 120 dental students who had healthy periodontium were taken and the photographic images were analyzed to be classified as 17 kinds of altered image groups. Results : The results demonstrated noticeable difference between the varying levels of discrepancy. Both group preferred gingival margin of lateral incisor to be 0.5mm lower than that of central incisor. Lay people preferred the gingival margin shape that has 2/9 horizontal component of the crown width, while dental professionals preferred the gingival margin shape that has 1/9 horizontal component of the crown width. Lay people preferred longer length of the interproximal contact (two thirds of the crown length), whereas dental professionals preferred shorter length of the interproximal contact (half of the crown length). Photographic analysis of normal esthetic gingival topography revealed 2/9 horizontal component and short length of the interproximal contact which was of the hybrid nature of the preferences shared by lay people and dental professionals. Conclusion: The results of this study show that dental professionals and lay people demonstrated significant difference in their preference of dental esthetic components, which may then influence the decision making process by dental professionals with respect to designing the anterior esthetic gingival line.

• Journal of Korean Neurosurgical Society
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• 제67권3호
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• pp.364-375
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• 2024

Objective : Kinesin family member C1 (KIFC1), a non-essential kinesin-like motor protein, has been found to serve a crucial role in supernumerary centrosome clustering and the progression of several human cancer types. However, the role of KIFC1 in glioma has been rarely reported. Thus, the present study aimed to investigate the role of KIFC1 in glioma progression. Methods : Online bioinformatics analysis was performed to determine the association between KIFC1 expression and clinical outcomes in glioma. Immunohistochemical staining was conducted to analyze the expression levels of KIFC1 in glioma and normal brain tissues. Furthermore, KIFC1 expression was knocked in the glioma cell lines, U251 and U87MG, and the functional roles of KIFC1 in cell proliferation, invasion and migration were analyzed using cell multiplication, wound healing and Transwell invasion assays, respectively. The autophagic flux and expression levels matrix metalloproteinase-2 (MMP2) were also determined using imaging flow cytometry, western blotting and a gelation zymography assay. Results : The results revealed that KIFC1 expression levels were significantly upregulated in glioma tissues compared with normal brain tissues, and the expression levels were positively associated with tumor grade. Patients with glioma with low KIFC1 expression levels had a more favorable prognosis compared with patients with high KIFC1 expression levels. In vitro, KIFC1 knockdown not only inhibited the proliferation, migration and invasion of glioma cells, but also increased the autophagic flux and downregulated the expression levels of MMP2. Conclusion : Upregulation of KIFC1 expression may promote glioma progression and KIFC1 may serve as a potential prognostic biomarker and possible therapeutic target for glioma.

• Journal of Nutrition and Health
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• 제40권4호
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• pp.347-361
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• 2007

The purpose of this study was to analyze and evaluate dietary intake between with and without hypertension. Study subjects were more than 30 years old adults (n = 3,806) who participated in the 2001 Korea National Health and Nutrition Examination Survey. People who have 'self-recognition about hypertension' and 'having diet for hypertension' were excluded. Using the WHO standard, subjects were divided into the hypertensive group (SBP> 140, DBP> 90) and the normal group (SBP< 140, DBP< 90). The Body Mass Index (BMI) and the Waist-Hip Ratio (WHR) of a hypertensive group were higher than those of a normal group (p < 0.01). The distribution of the subjects for smoking, alcohol consumption, exercise, stress, preference of salty food were not significantly different between a normal group and a hypertensive group (p>0.05). Dietary intakes were investigated by the 24-hour recall method. When food and dish intakes analyzed by sociodemographic factors, normal group consumed more fruits than those of a hypertensive group. Statistical significant were shown at female group, residences in metropolitan area and having elementary school education (p<0.05). Hypertensive group consumed more alcoholic beverages than those of a normal group at 'age 39-39', 'aged 50-64', 'high economic status', 'low economic status' and 'residences in metropolitan' (p<0.05) The amount of intakes for fat, potassium, thiamin, vitamin C and alcohol were significantly different between the normal group and the hypertensive group (p<0.05). The highest score of the Dietary diversity score (DDS) was 4 in both normal group and hypertensive group. Normal group showed high Nutrition Density (ND) of vitamin C and hypertensive group showed high ND of sodium. In summary, these results showed that significant difference for people with hypertension were intakes of fruits, alcohol, thiamin, vitamin C. And these results differed by sociodemographic groups. Therefore, the differential approach in each group is demanded for prevention and control of the hypertension.

• 보건행정학회지
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• 제30권4호
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• pp.493-504
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• 2020

Background: There have been many studies on the associations between body mass index (BMI) and cognitive function. However, no study has ever compared the associations across the methods of categorizing BMI. In this study, we aimed to fill the gap in the previous studies and examine whether the obesity paradox is valid in the risk of cognitive function. Methods: Of the 10,254 people aged 45 and older from the Korean Longitudinal Study of Ageing from 2006 to 2016, 8,970 people were finalized as the study population. The dependent variable was whether a person has a normal cognitive function or not, and the independent variables of interest were BMI categorized by the World Health Organization Western Pacific Regional Office (WHO-WPRO) method, the WHO method, and a 10-group method. Covariates included sociodemographic factors, health behavior factors, and health status factors. A generalized linear mixed model analysis with a logit link was used. Results: In the adjusted model with all covariates, first, in the case of BMI categories of the WHO-WPRO method, underweight (odds ratio [OR], 1.16; 95% confidence interval [CI], 1.15-1.17), overweight (OR, 1.36; 95% CI, 1.35-1.36), and obese (OR, 1.34; 95% CI, 1.33-1.34) groups were more likely to have a normal cognitive function than a normal-weight group. Next, in the case of BMI categories of the WHO method, compared to a normal-weight group, underweight (OR, 1.15; 95% CI, 1.14-1.16) and overweight (OR, 1.06; 95% CI, 1.06-1.07) groups were more likely to have a normal cognitive function; however, obese (OR, 0.62; 95% CI, 0.61-0.63) group was less likely to have it. Lastly, in the case of the 10-group method, as BMI increased, the likelihood to have a normal cognitive function changed like a wave, reaching a global top at group-7 (26.5 kg/㎡ ≤ BMI <28.0 kg/㎡). Conclusion: The associations between BMI and cognitive function differed according to how BMI was categorized among people aged 45 and older in Korea, which suggests that cognitive function may be positively associated with BMI in some categories of BMI but negatively in its other categories. Health policies to reduce cognitive impairment need to consider this association between BMI and cognitive function.

• The Korean Journal of Physiology and Pharmacology
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• 제19권2호
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• pp.83-88
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• 2015

CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) have been shown to play a regulatory or suppressive role in the immune response and are possibly relevant to the pathogenesis of autoimmune diseases. In the present study, we attempted to investigate the frequency of CD4+CD25+ Tregs in peripheral blood (PB) of collagen-induced arthritis (CIA) rats during the development of arthritis, to determine whether their frequency is involved in the immunoregulation of this disease. The results showed that normal rats had similar frequencies of CD4+CD25+ Tregs in PB during the experiment time, expressed as a percentage of CD4+CD25+Foxp3+ T cells among the CD4+ T lymphocyte population. In contrast, the frequency of CD4+CD25+Foxp3+ T cells in CIA rats was found to change during the development of arthritis. In CIA rats, there is a significant negative correlation between the frequency of CD4+CD25+Foxp3+ T cells and paw swelling (r=-0.786, p< 0.01). The relationship between the frequency of CD4+CD25+Foxp3+ T and immune activation was not found in normal rats. During the time course, the frequency of CD4+CD25+Foxp3+ T was lower in CIA rats than in normal ones. The data suggest that the frequency of PB CD4+CD25+ Tregs may be a promising marker for arthritis activity.

• 한국임상보건과학회지
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• 제5권1호
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• pp.816-824
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• 2017

Purpose. This study was classified into normal and demented elderly through K-MMSE. The purpose of this study was to analyze gait characteristics of normal elderly and demented peoples using GAITRite walking system. Methods. The subjects of this study were selected as elderly people receiving home visit physical therapy. An independent t-test was conducted to verify the statistical significance of the time-space variables of the elderly with dementia. Results. Step time(p=0.041), cycle time(p=0.037), distance(p=0.024), and cadence(p=0.048) were significantly shorter in the normal elderly than in the demented elderly on flat place. The mean age was significantly longer in normal elderly than in elderly persons with dementia. Step time(p=0.022), cycle time(p=0.023), distance(p=0.019), and cadence(p=0.015) were significantly shorter in the mat walking. The mean age was significantly longer in normal elderly than in elderly patients with dementia. Stretch time, cycle time, distance, and hair support time were significantly shorter in the mat walking. The mean age of the elderly was significantly longer than that of the elderly with dementia. The spinal support time, which is a spatial variable, was significantly shorter in the normal elderly than in the demented elderly. Conclusions. It compares the various gait characteristics of the normal and demented elderly people, thereby increasing the walking ability of the elderly person more effectively. This study should be utilized as basic data for preventing fall-down.

• The Journal of Korean Physical Therapy
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• 제15권3호
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• pp.91-109
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• 2003

The grip strength is orthopedics department, the place where from rehabilitation medical science and industrial medical science and it evaluates the function of the hand certainly is the ability which is necessary. There is specially by an occupation rehabilitation of mentally retarded child and the evaluation of grip strength is one which is meaning. The samples for this study were 39 normal males and 59 normal females, 62 mentally retarded males, 33 mentally retarded females ranging in age from Prepubeal period to Adolescence. This study was used to measure grip strength BASELINE Hydraulic Hand Dynamometer manufactured by EEI(Fabrication Enterprises Incorporated)in USA. The objective of research from the Prepubeal period to Adolescence the normal and mentally retarded students comparison evaluates the grip strength and effective fine motor program of one's it makes with the fundamental data for a development and it does. The results are as follow : (1) The hand which the normal student and the schoolgirl of rises from grip strength measurement result of the Prepubeal period research object people 10.7Kg with the same left hand was each measured 10Kg with 9.8Kg with in. The grip strength of the mentally retarded schoolboys was the hand which rises 6Kg with the left hand 5.4Kg, the grip strength of the schoolgirls 6.5Kg with was identical. (2) The hand which the normal schoolboys and the schoolgirls rises from grip strength measurement result of the Adolescence research object people 29.6Kg, 20Kg the left hand to be was each measured 27.8Kg with 18.4Kg with. The hand which the mentally retarded schoolboys and the schoolgirls rises 13.1Kg, 11.3Kg the left hand to be was each measured 12.3Kg with 10.9Kg with. (3) Among the normal students $10.2\%$ (Prepubeal period $0.94\%$, Adolescence $15.2\%$) in Mentally Retarded students $17.9\%$ (Prepubeal period $0.77\%$, Adolescence $19.1\%$) left hand it was higher. (4) The grip strength of the students who attend school from the general family dwells were appeared higher than students residence at institution.

• 대한영양사협회학술지
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• 제17권4호
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• pp.378-386
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• 2011

To examine the effect of obesity on volume perception according to size and color of rice bowl, we divided female college students into a normal weight group (<30% fat mass, n=100) and obese group (${\geq}30%$ fat mass, n=83) and then measured perceived volume of rice bowls of various sizes (general size; 350 ml vs. small size; 188 ml) and color (yellow, white, blue, and black) containing the same amount of cooked white rice (210 g). Normal weight group perceived that the general rice bowl contained significantly more cooked white rice compared to the small rice bowl. In contrast, the obese group perceived that the general rice bowl contained significantly less cooked white rice than the small rice bowl. The estimated variance in perceived volume of both bowls was significantly bigger in the obese group compared to the normal group. There were no differences in perceived volume among any of the subjects (both normal and obese groups) according to rice bowl color. However, the estimated variance in perceived volume in the obese group was significantly larger than that in the normal group for all of the rice bowls. In conclusion, rice bowl size and color might affect volume perception, and volume perception in obese people may be different from that of normal weight people.

• 동의생리병리학회지
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• 제23권3호
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• pp.723-733
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• 2009

The purpose of this study is to evaluate the difference about pathogenesis of normal group and depression group(who' pulmonary function is below expected value). Author used DSOM to investigate oriental pathogenesis. Depression group is consisted of people who's FVC%(forced vital capacity)is below 80% or $FEV_1$/FVC%(Forced Expiratory Volume in 1sec/FVC) is below 70%, and they don't have history of lung disease(Athma, tuberculosis, COPD, suchlike). Normal group is consisted of people who's FVC%(Forced Vital Capacity) is in 80-120% and $FEV_1$/FVC%(Forced Expiratory Volume in 1sec/FVC) is above 70%. they also don't have history of lung disease. Author carried out each group's PFT(pulmonary function test) by ATS(American Thoracic Society) method. DSOM was used for oriental pathogenesis investigation of two groups. There was significant difference between normal group and depression group in Kidney(p<0.05). In depression group comparison of sex, there was significant difference between male and female in stagnation of qi, cold, heat, spleen, phlegm(p<0.05). In Normal group there was significant difference between male and female in stagnation of qi, blood stasis, cold, heat, spleen(p<0.05). In depression group comparison of smoke, there was no significant difference between smoker and non-smoker(p<0.05). In Normal group comparison of smoke, there was significant difference between smoker and non-smoker in heat(p<0.05). This result showed difference of the pathogenesis between Depression group and Normal group.