• 한국지역지리학회지
• /
• 제13권6호
• /
• pp.639-650
• /
• 2007

이 글은 나주 광주 전남 공동혁신도시의 건설에 즈음하여 이전대상 공공기관의 직원들이 가족과 함께 혁신도시로의 이전 및 정착 의사에 관해 분석한 것이다. 이 글은 혁신도시 건설에 따른 지역사회에 대한 파급효과를 극대화하기 위해서는 우선 이전 공공기관의 종사자 및 그 가족들의 이주와 조기 정착이 이뤄져야 한다는 논점에 입각하여 분석을 수행하였다. 분석 결과를 살펴보면, 이주 및 정착 가능성에 대한 부정적 응답 비율이 절반 가까이 높은 것으로 나타났으며, 또한 혁신도시의 현재와 미래에 대해서도 다소 비관적 의견이 높았다. 따라서 혁신도시로의 이주 및 정착을 유도하기 위해서는 특히 교육여건과 아울러 요양 및 실버타운 등의 각종 생활서비스 시설들의 중점적인 확충이 필요한 것으로 밝혀졌다.

• Journal of Microbiology and Biotechnology
• /
• 제26권2호
• /
• pp.356-363
• /
• 2016

Human papillomavirus (HPV), a non-enveloped, double-stranded DNA tumor virus, is a primary etiological agent of cervical cancer development. As a potential tool for prophylactic vaccination, the development of virus-like particles (VLPs) containing the HPV16 L1 capsid protein is highly desired. In this study, we developed a high-level expression system of the HPV16 L1 in Escherichia coli for the purpose of VLP development. The native gene of HPV16 L1 has many rare codons that cause the early termination of translation and result in the production of truncated forms. First, we optimized the codon of the HPV16 L1 gene to the preferable codons of E. coli, and we succeeded in producing the full-size HPV16 L1 protein without early termination. Next, to find the best host for the production of HPV16 L1, we examined a total of eight E. coli strains, and E. coli BL21(DE3) with the highest yield among the strains was selected. With the selected host-vector system, we did a fed-batch cultivation in a lab-scale bioreactor. Two different feeding solutions (complex and defined feeding solutions) were examined and, when the complex feeding solution was used, a 6-fold higher production yield (4.6 g/l) was obtained compared with that with the defined feeding solution.

• 한국보육지원학회지
• /
• 제9권5호
• /
• pp.181-200
• /
• 2013

본 연구의 목적은 유아교육을 전공하는 학생들에게 영어로 유아수학교육 수업을 제공하는 과정에서 단계별로 교수자가 구성한 어려움과 의미를 알아보는 것이었다. 내러티브 탐색법을 활용하여 질적 연구를 실행하였으며, 관찰, 인터뷰, 학생들이 작성한 반성적 저널이 연구 자료로 수집되었다. 그 결과, 우선, 준비하는 단계에서 교수자는 원어민이 아니지만, 영어로 수업을 해야 한다는 압박과 영어로 수학교육내용을 어떻게 효과적으로 알려줄 수 있는 다양한 방법을 고려하였다. 수업 하는 단계에서 교수자는 학생들의 지속적인 동기 유발을 갖도록 하는 것과 교수자와 학생, 학생들 간 상호작용이 일어날 수 있는 기회를 마련하는 것을 중요하게 인식하였다. 평가 단계에서는 수업 준비와 실행 단계에서 부족했던 내용을 분석하는 것을 중요하게 인식하였다. 본 연구의 결과는 유아교육학과에서 영어로 전공 수업을 하는 상황에서 의미 있고, 적절한 교수학습 방법을 찾아보는 것에 기여를 할 것이라 사려된다.

• 한국수정란이식학회지
• /
• 제12권1호
• /
• pp.21-27
• /
• 1997

The studies were carried out to investigate the effects of bisection method and with and without-zona pellucida of embryos on in vitro developmental rate bisected embryos by micromanipulator, micropipette and pipetting. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 mediurn containing 10 IU /ml의 PMSG(Sigma, USA), 10 IU /ml의 hCG, 1$\mu$g /ml의 $\beta$-estradiol(Sigma, USA) and 10% FCS for 24~48 hrs in incubator with 5% $CO_2$ in air at 38.5$^{\circ}C$ and then, matured oocytes were again cultured for 12~ 18 hrs with motile capacitated sperm by preincubation of heparin. Bisected embryos cultured for 1~5 days in 20% FCS + TCM-199 medium. Survival rate was defined as developmental rate on in vitro culture or FDA-test. The results are summarized as follows :1. The survival rates of bisected bovine embryos by micromanipulator and micropipett were 29.2% and 19.1%, respectively. The rates of non-bisection embryos(46.7%) were significantly higher than those of bisection embryos. 2. The in vitro developmental rates of bisected bovine embryos by micromanipulator, micropipett and pipetting method were 32.4%, 19.4% and 25.6%, respectively.3. The in vitro developmental rates of with and without-zona pellucida of bisected bovine embryos by raicromanipulator were 30.8% and 25.0%, respectively. The rates of nonbisection embryos(53.1%) were significantly higher than those of bisection embryos.

• 한국산학기술학회논문지
• /
• 제12권2호
• /
• pp.640-645
• /
• 2011

본 논문의 목적은 영어 구술시험에서 클로즈 테스트가 더욱 효과적으로 활성화될 수 있는 방법을 조사하여 영어를 외국어로 학습되는 환경에서 영어가 모국어가 아닌 교사들이 클로즈 테스트를 더욱 활용할 수 있게 하기 위함이다. 이는 클로즈 테스트에서 사용되는 자료, 삭제율, 항목의 특징, 그리도 점수 계산 방식을 조사하는 것이다. 테스트의 효율성을 향상시키는 것은 어떻게 위의 네 가지 요소가 클로즈 테스트의 결과에 영향을 끼치는 지를 파악 함으로써, 그리고 그것들을 어떻게 잘 조절할 수 있음으로써 가능할 수 있다. 본 논문은 교사들이 네 가지 요소들을 능숙하게 다루어 클로즈 테스트의 효율성과 신뢰성을 발전시킬 수 있도록 몇 가지 제안을 하였다. 결국 중요한 것은 교사들이 테스트의 결과에 영향을 주는 요소들을 잘 알고, 그들의 목표에 알맞은 테스트를 만들어 내기 위해 네가지 요소들을 잘 조절함으로써, 클로즈 테스트의 효율성과 신뢰성을 높일 수 있다는 것이다.

• 대한한방피부미용학회지
• /
• 제1권1호
• /
• pp.98-113
• /
• 2005

This paper provides comparison and analysis between brand images of Korean herbal cosmetics and general, imported cosmetic brands, focusing on product's symbolic and functional images. The objective is to evaluate brand images of Korean herbal cosmetics to suggest a powerful brand image strategy that will be different and competitive. Data collection was performed on 300 adult women who were over 20 years of age and lived in Seoul or KyongKi area. Analysis was carried out using SPSS version 11.5 and frequency analysis, T-Test and $X^2$ verification was also performed. Analysis results for brand images of Korean herbal cosmetics are as follows. First, satisfaction scale as a purchase feature for users of Korean herbal cosmetics were 'Normal' 51.5%, 'Satisfactory' 35.1%, 'Very Satisfactory' 8.2% and 'Unsatisfactory' 5.2%. Regarding improvements, 'Efficacy, Effect' was the highest with 46%. Second, 'SEOLHWASOO' and 'BAEKOKSENG' among Korean herbal cosmetic brands, 'IOPE' and 'ISA KNOX' among general cosmetic brands and 'LANCOME' and 'CHANEL' among imported cosmetic brands displayed high recognition. Third, the symbolic and functional images of Korean herbal cosmetic brands were seen to be better compared to their general and imported counterparts. Especially, product related features of Korean herbal cosmetic brands received a more positive response compared to imported brands, which suggests that with research and improvement regarding non-product related features, functional images of herbal brands may be appraised ahead of imported brands. Fourth, there is a significant difference in brand preference according to the satisfaction of groups who actually use herbal cosmetics and the purchasing attitudes of groups who do not. Therefore, an active relationship marketing strategy is required to maximize satisfaction and to elicit favorable attitudes. By developing functional native ingredients, constructing data that can prove its efficacy and effects, and appealing its unique, differentiated symbolic value together with Orientalism, herbal medicines will be able to take off to be acknowledged worldwide.

• 한국임상수의학회지
• /
• 제33권5호
• /
• pp.266-269
• /
• 2016

Cell-free fetal RNA is useful to determine fetal sex and detect other inherent genetic disorders. However, non-invasive fetal sex determination methods using fetal RNA from maternal plasma is not yet well established in studies pertaining to bovine animals. Thus, the aim of this study was to systematically evaluate the presence of the male-specific ZRSR2Y gene transcript in maternal plasma using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assays, and to verify its accuracy, sensitivity, and specificity in determining fetal sex between 30 and 100 days of gestation. Overall accuracy, sensitivity, and specificity of the ZRSR2Y gene transcripts in determining fetal sex were 89.1%, 86.3%, and 100%, respectively. The 30 to 100 days of gestation were further classified into five stages of gestation, and each stage had relatively high accurate, sensitive, and specific results. Overall, these results indicate that the expression of the ZRSR2Y gene can be used for fetal sex determination in bovine animals using circulating cell-free RNA in maternal plasma during early pregnancy.

• 생체재료학회지
• /
• 제22권4호
• /
• pp.235-248
• /
• 2018

Background: Injectable hydrogels have been extensively researched for the use as scaffolds or as carriers of therapeutic agents such as drugs, cells, proteins, and bioactive molecules in the treatment of diseases and cancers and the repair and regeneration of tissues. It is because they have the injectability with minimal invasiveness and usability for irregularly shaped sites, in addition to typical advantages of conventional hydrogels such as biocompatibility, permeability to oxygen and nutrient, properties similar to the characteristics of the native extracellular matrix, and porous structure allowing therapeutic agents to be loaded. Main body: In this article, recent studies of injectable hydrogel systems applicable for therapeutic agent delivery, disease/cancer therapy, and tissue engineering have reviewed in terms of the various factors physically and chemically contributing to sol-gel transition via which gels have been formed. The various factors are as follows: several different non-covalent interactions resulting in physical crosslinking (the electrostatic interactions (e.g., the ionic and hydrogen bonds), hydrophobic interactions, ${\pi}$-interactions, and van der Waals forces), in-situ chemical reactions inducing chemical crosslinking (the Diels Alder click reactions, Michael reactions, Schiff base reactions, or enzyme-or photo-mediated reactions), and external stimuli (temperatures, pHs, lights, electric/magnetic fields, ultrasounds, or biomolecular species (e.g., enzyme)). Finally, their applications with accompanying therapeutic agents and notable properties used were reviewed as well. Conclusion: Injectable hydrogels, of which network morphology and properties could be tuned, have shown to control the load and release of therapeutic agents, consequently producing significant therapeutic efficacy. Accordingly, they are believed to be successful and promising biomaterials as scaffolds and carriers of therapeutic agents for disease and cancer therapy and tissue engineering.

• 한국자원식물학회지
• /
• 제14권3호
• /
• pp.277-285
• /
• 2001

본 연구에서는 제주도에서 자라고 있는 귀화식물의 생활형, 원산지, 도입시기를 파악하였다. 본 연구는 현지조사를 통하여 이루어졌으며 그 결과를 요약하면 아래와 같다. 1. 본 연구에서는 귀화식물이란 “우리나라 비토착종으로서 인위적 또는 자연적인 방법으로 우리나라에 들어와 야생상태에서 스스로 번식하여 생존할수 있는 종”으로 정의(定議)하여 처리하였다. 2. 제주도에 자라는 귀화식물의 종류는 31과 111속 178종 10변종의 188종류로 조사 정리하였다. 과별로는 국화科 43種類(23%), 벼科 27種類(14%), 콩과 15種類(8%), 십자화과 15종류(8%)의 높은 비율을 차지하고 있었으며, 원산지별로는 아메리카가 가장 많았다. 그 다음으로는 유럽 의 원산지인 종이 많았다. 3. 제주도의 귀화식물 188종류의 분포지를 파악하였는데, 이를 토대로 보면 전국적으로 분포하는 종은 97종류이었고, 제도에만 분포하는 종은 37종류이었다. 4. 제주도의 귀화식물 원산지별로는 유럽64(35%) 종류이고, 아메리카지역 85(45%)종류, 아시아지역 19(10%)종류순으로 나타난다.

• Journal of Microbiology and Biotechnology
• /
• 제31권4호
• /
• pp.570-583
• /
• 2021

Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.