• Journal of Nutrition and Health
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• 제44권1호
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• pp.16-28
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• 2011

Oxidative stress leads to the induction of cellular oxidative damage, which may cause adverse modifications of DNA, proteins, and lipids. The production of reactive species during oxidative stress contributes to the pathogenesis of many diseases. Antioxidant defenses can neutralize reactive oxygen species and protect against oxidative damage. The aim of this study was to assess the antioxidant status and the degree of DNA damage in Korean young adults using glutathione s-transferase (GST) polymorphisms. The GSTM1 and GSTT1 genotypes were characterized in 245 healthy young adults by smoking status, and their oxidative DNA damage in lymphocytes and antioxidant status were assessed by GST genotype. General characteristics were investigated by simple questionnaire. From the blood of the subjects, GST genotypes; degree of DNA damage in lymphocytes; the erythrocyte activities of superoxide dismutase, catalase, and glutathione peroxidase; plasma concentrations of total peroxyl radical-trapping potential (TRAP), vitamin C, ${\alpha}$- and ${\gamma}$-tocopherol, ${\alpha}$- and ${\beta}$-carotene and cryptoxanthin, as well as plasma lipid profiles, conjugated diene (CD), GOT, and GPT were analyzed. Of the 245 subjects studied, 23.2% were GSTM1 wild genotypes and 33.4% were GSTT1 wild genotype. No difference in erythrocyte activities of superoxide dismutase, catalase, or glutathione peroxidase, and the plasma TRAP level, CD, GOT, and GPT levels were observed between smokers and non-smokers categorized by GSTM1 or GSTT1 genotype. Plasma levels of ${\alpha}$- and ${\gamma}$-tocopherol increased significantly in smokers with the GSTT1 wild genotype (p < 0.05); however, plasma level of ${\alpha}$-carotene decreased significantly in non-smokers with the GSTM1 wild genotype (p < 0.05). DNA damage assessed by the Comet assay was significantly higher in non-smokers with the GSTM1 genotype; whereas DNA damage was significantly lower in non-smokers with the GSTT1 genotype. Total cholesterol and LDL cholesterol levels were significantly higher in non-smokers with the GSTT1 genotype than those with the GSTT1 wild genotype (p < 0.05). In conclusion, the GSTM1 genotype or the GSTT1 wild genotype in non-smokers aggravated their antioxidant status through DNA damage of lymphocytes; however, the GSTT1 wild type in non-smokers had normal plasma total cholesterol and LDL-cholesterol levels. This finding confirms that GST polymorphisms could be an important determinant of antioxidant status and plasma lipid profiles in non-smoking young adults. Further study is necessary to clarify the antioxidant status and/or lipid profiles of smokers with the GST polymorphism and to conduct a study with significantly more subjects.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2003년도 제31차 춘계 학술대회
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• pp.1-14
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• 2003

Lipid oxidation is one of the most important non-microbial causes of meat quality deterioration. However, there have been different/conflicting views concerning the primary catalysts of lipid oxidation in meat. This presentation provides brief overviews of lipid oxidation mechanism in general and catalysis of lipid oxidation in meat, and then focuses on inter-species differences in lipid oxidation potential, using results from our studies on meats (beef, pork and chicken) at retail and the respective meats of uniform postmortem history. The inter-species differences have highlighted the relative roles of meat pigment (myoglobin) content, catalase activity, and the concentration of oxidation substrates (particularly polyunsaturated fatty acids) in determining the lipid oxidation potential of raw meat versus cooked meat.

• Natural Product Sciences
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• 제1권1호
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• pp.17-24
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• 1995

The effects of orchildectomy with/without testosterone replacement in wale rats, and ovarectomy with estrogen replacement in female rats on lipid peroxidation were studied in male and female rats fed with diets fortified with 20% w/w, soybean oil or palm oil for 4 months. Serum, liver and heart homogenates were assayed for malonaldehyde and conjugated diene levels. Orchidectomy was found to reduce levels of lipid peroxidation products in the serum, liver and heart. Testosterone replacement did not increase the lipid peroxidation products to levels in the non-orchildectomised rats, while estrogen did not influence lipid peroxidation significantly. Palm oil decreased, but soybean oil increased lipid peroxidation in the liver and heart of both the castrated and sex hormone-replaced male and female rats.

• 한국수산과학회지
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• 제17권4호
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• pp.313-320
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• 1984

고등어 지질의 산화안정성을 밝히기 위하여 어체를 표피, 보통육, 혈합육 및 내장의 4부위와 총어체에서 지질을 추출하여, $30^{\circ}C$에 저장하여 두고 산가, 과산화물가, TBA가, 요오드가, 중량변화 및 지방산 조성 등의 변화를 경시적으로 조사하여 산화안정성을 검토하였는데, 그 결과는 다음과 갈다. 1. 저장 중 지질의 과산화물가, TBA가의 변화 및 중량변화등으로 보아 산화속도는 표피지질, 내장지질, 총지질, 혈합육지질, 보통육지질의 순으로 빨랐다. 2. 각 부위의 극성지질과 비극성지질의 비율은 혈합육지질의 극성지질이 $15.46\%$로 가장 많았고, 표피지질의 극성지질이 $5.35\%$로 가장 적었다. 3. 전지질의 지방산 조성은 각 부위 모두 $C_{16:0}$산, $C_{18:1}$산 및 $C_{22:6}$산이 많았으며, 고도불포화산인 $C_{22:6}$산은 혈합육지질이 가장 많았고 표피지질이 가장 적었다. 4. 극성지질과 비극성지질의 지방산 조성에 있어서 $C_{16:1}$산 및 $C_{18:1}$산의 monoene산은 극성지질이 비극성지질에 비하여 낮았으며, $C_{20:5}$산 및 $C_{22:6}$산의 polyene산은 극성지질이 비극성지질보다 높았다. 5. 저장 중 지방산 조성의 변화는 polyene산 특히, $C_{20:5}$산 및 $C_{22:6}$산이 현저한 감소를 보였으며, $C_{14:0}$산 및 $C_{16:0}$산의 포화산의 증가가 두드러졌고 polyene산의 감소는 극성지질이 비극성지질보다 감소 속도가 빨랐다.

• IMMUNE NETWORK
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• 제23권4호
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• pp.28.1-28.20
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• 2023

Lipid accumulation in macrophages is a prominent phenomenon observed in atherosclerosis. Previously, intimal foamy macrophages (FM) showed decreased inflammatory gene expression compared to intimal non-foamy macrophages (NFM). Since reprogramming of lipid metabolism in macrophages affects immunological functions, lipid profiling of intimal macrophages appears to be important for understanding the phenotypic changes of macrophages in atherosclerotic lesions. While lipidomic analysis has been performed in atherosclerotic aortic tissues and cultured macrophages, direct lipid profiling has not been performed in primary aortic macrophages from atherosclerotic aortas. We utilized nanoflow ultrahigh-performance liquid chromatography-tandem mass spectrometry to provide comprehensive lipid profiles of intimal non-foamy and foamy macrophages and adventitial macrophages from Ldlr^-/- mouse aortas. We also analyzed the gene expression of each macrophage type related to lipid metabolism. FM showed increased levels of fatty acids, cholesterol esters, phosphatidylcholine, lysophosphatidylcholine, phosphatidylinositol, and sphingomyelin. However, phosphatidylethanolamine, phosphatidic acid, and ceramide levels were decreased in FM compared to those in NFM. Interestingly, FM showed decreased triacylglycerol (TG) levels. Expressions of lipolysis-related genes including Pnpla2 and Lpl were markedly increased but expressions of Lpin2 and Dgat1 related to TG synthesis were decreased in FM. Analysis of transcriptome and lipidome data revealed differences in the regulation of each lipid metabolic pathway in aortic macrophages. These comprehensive lipidomic data could clarify the phenotypes of macrophages in the atherosclerotic aorta.

• 대한물리의학회지
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• 제8권2호
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• pp.239-243
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• 2013

PURPOSE: The purpose of present study was to investigate effects of obesity, blood pressure and life style on lipid indices and blood pressure in men of age 40s. METHODS: One hundred forty five subjects in men of age 40s were participated in this study. All participants were taken physical examination, lifestyle survey and laboratory test. According to examination, participants were divided into two group in four categories ; obesity and normal group, hypertension and normal group, smokers and non-smokers, and drinkers and non-drinkers. The low density lipoprotein cholesterol (LDL), high density lipoprotein cholesterol (HDL), total cholesterol (TC), triglyceride (TG), systolic blood pressure (SBP), diastolic blood pressure (DBP) were analyzed by independent t-test for comparison between two groups. RESULTS: The values of LDL, TC, SBP, DBP were higher and HDL was lower in obesity than in normal group (p<.05). The values of LDL, TC, SBP, DBP were higher in hypertension group than in normal group (p<.05). The values of TG was higher and HDL was lower in smokers than in non-smokers (p<.05). There's no significant differences between drinkers and non-drinkers (p>.05). CONCLUSION: It can be seen that obesity and blood pressure were more relative risk factors than smoking and alcohol indices in cardiovascular diseases.

• Nutritional Sciences
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• 제9권4호
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• pp.273-279
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• 2006

The purpose of this study was to determine the independent and the combined effects of cell cultured Acanthopanax senticosus extracts (ASE) supplementation and swimming exercise on body weight, lipid profile, carnitine and leptin levels in C57 BL/6J mice. Forty C57BL/6J mice were divided into four groups: non-supplement and non-exercise (NSNE); non-supplement and exercise (NSE); supplement and non-exercise (SNE); supplement and exercise (SE) mice. They were allowed free access to food and water. The exercised groups were forced to swim (1hr, 6 days a week) in a water bath for 12 weeks. The supplemented groups were fed Cell cultured ASE (0.5 g/kg body weight/day) for 12 weeks. In this study, we found that the combination of Cell cultured ASE supplementation and exercise significantly decreased liver triglyceride (TG) level and serum leptin level but significantly increased serum HDL-cholesterol level compare to control (NSNE) group. These improved lipid profiles and decreased serum leptin would have positive effects on obesity and cardiovascular disease.

• International Journal of Internet, Broadcasting and Communication
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• 제11권4호
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• pp.1-10
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• 2019

This Dietary cholesterol augments lipid profile and primes production and activation of platelets, leading to development of atherosclerosis which produce several detrimental effects on cardiovascular health. Ethnomedicine and Mediterranean diet are natural sources and cost effective modes against several ailments including cardiovascular diseases while fermented foods have gained interest due to their increased nutrient profile, enhanced bioavailability and efficacy. Garlic has been known to reduce cholesterol and inhibit platelet activation. We examined whether fermented garlic ameliorates effects of hypercholesterolemia and platelet functions in rats. Methodology: Male SD rats were fed with hypercholesterolemia diet and treated with spirulina, fermented and non-fermented preparations of garlic for one month. Platelet aggregation and granule secretion were assessed to evaluate platelet activation. Liver and kidney weights, lipid and enzymatic profile of serum and whole blood analysis was performed. Expressions of SREBP, ACAT-2 and HMG-CoA were assessed using RT-PCR while liver and adipose tissues were analyzed for histological changes. Both fermented and non-fermented garlic inhibited platelet aggregation and granule secretion while fermented garlic showed greater inhibitor tendency. Fermented garlic significantly reduced liver weight and triglycerides concentrations than non-fermented garlic. Similarly, fermented garlic greatly abrogated the detrimental effects of steatosis on liver and adipose tissues. Fermented garlic significantly improved lipid profile and modulated platelet functions, thereby inhibiting atherosclerosis and platelet related cardiovascular disorders.

• BMB Reports
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• 제36권2호
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• pp.190-195
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• 2003

The Vitamin E (VE) effect was examined on oxidative damage to DNA, lipids, and protein in mice that were fed various levels of lipid diets after total body irradiation (TBI) with X-rays at 2 Gy. No increase of 8-hydroxydeoxyguanosine (8OHdG) by TBI was observed in the +VE group; however, in the case of the -VE group, a significantly higher 8OHdG level was observed in the high-lipid group than in the low- or basal-lipid group. In the groups with TBI, the concentration of thiobarbituric reactive substances (TBARS) only significantly increased in the high-lipid (-VE) group. These changes in TBARS, due to TBI, were not detected in other groups. The contents of protein carbonyls only increased in the (-VE) group. The contents of protein carbonyls was significantly different between the (+VE) and the (-VE) groups, regardless of the lipid levels. The concentrations of GSH, vitamins C and E in the liver were lower, and the concentration of non-heme iron in the liver was higher in the high-lipid group than in the low- and basal-lipid groups. These concentrations in the high-lipid group were significantly different between the (+VE) and the (-VE) groups. These results strongly suggest that mice that are fed a high-lipid diet are susceptible to TBI-induced oxidative damage. Also, decreases in the GSH levels and an increase in the iron level are involved in the mechanism of this susceptibility.

• 대한화학회지
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• 제67권2호
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• pp.89-98
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• 2023

The cell membrane, also known as the biological membrane, surrounds every living cell. The main components of cell membranes are lipids and therefore called as lipid membranes. These membranes are mainly made up of a two-dimensional lipid bilayer along with integral and peripheral proteins. The complex nature of lipid membranes makes it difficult to study and hence artificial lipid membranes are prepared which mimic the original lipid membranes. These artificial lipid membranes are prepared from phospholipid vesicles (liposomes). The liposomes are formed when self-forming phospholipid bilayer comes in contact with water. Liposomes can be unilamellar or multilamellar vesicles which comprises of phospholipids that can be produced naturally or synthetically. The phospholipids are non-toxic, biodegradable and are readily produced on a large scale. These liposomes are mostly used in the drug delivery systems. This paper offers comprehensive literature with insights on developing basic understanding of lipid membranes from its structure, organization, and phase behavior to its potential use in biomedical applications. The progress in the field of artificial membrane models considering methods of preparation of liposomes for mimicking lipid membranes, interactions between the lipid membranes, and characterizing techniques such as UV-visible, FTIR, Calorimetry and X-ray diffraction are explained in a concise manner.