• Title/Summary/Keyword: non-animal test

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Effects of Tannery Wastes on the Fattening of Growing Cattle, Carcass, and Meat Quality

  • Alam, Jahangir;Hossain, Mufazzal;Beg, Anwarul Haque;Nam, Ki-Chang;Lee, Sang-Suk
    • Food Science of Animal Resources
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    • v.30 no.2
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    • pp.190-197
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    • 2010
  • The present study was conducted to determine the effect of tannery waste protein concentrate (TWPC) on fattening of cattle and the carcass and meat quality, with the aim of replacing the costly commercial protein concentrate (Jasoprot) with a more economical and effective alternative. Twelve young cattle (six male and six female) were fed during the study period on a control diet (T1) with 10% Jasoprot and on two test diets: 5% TWPC + 5% Jasoprot (T2) and 10% TWPC (T3). The test diets significantly affected (p<0.05) live weight gain and profitability compared to the control diet, perhaps due to the increased protein and essential amino acid content, relative to Jasoprot. TWPC was free of aflatoxin. Sensory-evaluated organoleptic scores did not differ among the groups. Chemical composition was normal as other beef and was non toxic especially within recommended chromium level ($1.90{\pm}0.6{\mu}g$) Total lipid contents were higher (p<0.05) in T3, and moisture, ash and crude protein contents were almost similar (p>0.05) among the three groups. It is concluded that TWPC or an equal mixture of TWPC and Jasoprot may be an economic and efficient alternative protein source to Jasoprot in the cattle industry, which minimizes adverse effects on carcass and sensory meat quality.

Standardization of the Emergence Time of a Protaetia brevitarsis Adult (흰점박이꽃무지(Protaetia brevitarsis) 성충의 우화되는 시기의 표준화)

  • Choi, In-Hag;Choi, Sung-Up;Son, Jin-Sung;Chung, Tae-Ho
    • Journal of Environmental Science International
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    • v.30 no.2
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    • pp.185-189
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    • 2021
  • This study was conducted to determine and to standardize the time of emergence of Protaetia brevitarsis adults, through four breeding experiments, including one which selected the dominant individuals of Protaetia brevitarsis. The breeding experiments were divided into two groups with hibernating and non-hibernating Protaetia brevitarsis by placing 100, 150, 200, 250 and 300 Protaetia brevitarsis in each breeding box (50 cm × 35 cm × 15 cm) filled with 12 L of fermented sawdust. Breeding tests were with four replicates each for 3 months. The test period was classified into four stages: Exp. 1 (Sep 1, 2019 to Nov 30, 2019), Exp. 2 (Dec 1, 2019 to Feb 29, 2020), Exp. 3 (Mar 1, 2019 to May 31, 2020) and Exp. 4 (Jun 1, 2020 to Aug 31, 2020). The survey items included average dead number, average emergence number, average mortality, and average emergence rate. In Exp. 1 and 2, the changes in Protaetia brevitarsis adults were similar. In Tables 1 and 2, the allegorical changes of adult white-spotted flowers were similar. In addition, the average dead number, average emergence number, average mortality, and average emergence rate showed better results in the hibernation state than in both Exp. 1 and 2. Exp. 3 and 4 showed a pattern of change different from the results of Exp. 1 and 2 and these characteristics were superior in the hibernation states compared to the non-hibernation states. In conclusion, this result was found to be most appropriate as a standardization of the emergence time of 200 Protaetia brevitarsis treatments through four breeding experiments in the hibernating state.

Sanitation and Tissue Residue Problems in High Quality Pork - Review -

  • Lee, M.H.;Ryu, P.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.2
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    • pp.233-243
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    • 1999
  • Food safety or sanitation are terms broadly applicable to procedures designed to ensure that food quality is high and free of factors which may adversely affect human health. These factors include zoonotic diseases and acute and chronic effects of ingesting natural and human-made xenobiotics. Use of drugs in animal production for the treatment and control of animal diseases, to promote growth rate, and to improve feed conversion efficiency has expanded year by year, thus increasing the possibilities for occurrences in animal products of residues harmful to humans. Governmental agencies have made efforts to control or prevent residue problems. The Korean Food and Drug Administration (KFDA) is charged with the responsibility of establishing tolerances for veterinary drugs, pesticides, and mycotoxins and other non-pharmaceutical substances. The Department of Veterinary Service is responsible for establishing guidelines regarding withdrawal times of drugs, approval of drugs, their uses, and sanitation enforcement of livestock products. The authors describe the toxicological basis for the establishment of tolerance levels for xenobiotics and the pharmacokinetic basis for establishing withdrawal time for veterinary drugs. The regulatory tolerance levels of chemicals in pork and swine feed, Korean regulations on the use of feed additives, rapid residue test methods, the National Residue Program, and the Food Animal Residue Avoidance Databank are discussed. Rapid EIA methods that are under development for the screening of live animals are described These methods predict tissue residues from an examination of blood samples taken from pigs before they are slaughtered.

Performance of growing lambs supplemented with ground licuri (Syagrus coronata)

  • Daza, Adin;Souza, Jocely G.;Monnerat, Joao Paulo I.S.;Ribeiro, Claudio V.D.M.
    • Animal Bioscience
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    • v.34 no.6
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    • pp.1014-1021
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    • 2021
  • Objective: The aim of this study was to determine the effect of dietary ground licuri on lamb performance. Methods: Forty male lambs were used in a completely randomized design to test the effects of 0, 5, 10, and 15 g/kg of ground licuri added to diets. The trial lasted for 75 days. Intake, digestibility, physically effective neutral detergent fiber, and chewing activity were estimated. Blood samples were taken on day 45 to determine the concentrations of glucose, urea, non-esterified fatty acids, and triglycerides. Average daily gain (ADG) were determined on the last day of the experimental trial. Results: Licuri inclusion markedly increased dietary neutral detergent fiber and ether extract (EE) content, but it decreased dry matter (DM) intake. However, the intake and digestibility of EE linearly increased. The ADG decreased linearly (p<0.05) with licuri inclusion. Licuri had no effect (p>0.05) on the concentrations of blood metabolites; however, blood urea increased (p<0.05), while serum glucose decreased (p<0.05). Conclusion: The physically effective fiber of ground licuri is similar to Tyfton hay and licuri inclusion decreases lamb performance due to a decreased in DM intake.

A comparative analysis of rumen pH, milk production characteristics, and blood metabolites of Holstein cattle fed different forage levels for the establishment of objective indicators of the animal welfare certification standard

  • Baek, Dong Jin;Kwon, Hyoun Chul;Mun, Ah Lyum;Lim, Joo Ri;Park, Sung Won;Han, Jin Soo
    • Animal Bioscience
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    • v.35 no.1
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    • pp.147-152
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    • 2022
  • Objective: This study was conducted to obtain an objective index that can be quantified and used for establishing an animal welfare certification standard in Korea. For this purpose rumen pH, ruminating time, milk yield, milk quality, and blood components of cows reared in farms feeding high forage level (90%) and farms feeding low forage level (40%) were compared. Methods: Data on rumen pH, rumination time, milk yield, milk fat ratio, milk protein ratio, and blood metabolism were collected from 12 heads from a welfare farm (forage rate 88.5%) and 13 heads from a conventional farm (forage rate 34.5%) for three days in October 2019. Results: The rumination time was longer in cattle on the welfare farm than on the conventional farm (p<0.01), but ruminal pH fluctuation was greater in the cattle on conventional farm than the welfare farm (p<0.01). Conventional farms with a high ratio of concentrated feed were higher in average daily milk yield than welfare farms, but milk fat and milk production efficiency (milk fat and milk protein corrected milk/total digestible nutrients) was higher in cattle on welfare farms. Blood test results showed a normal range for both farm types, but concentrations of total cholesterol and non-esterified fatty acid were significantly higher in cows from conventional farms with a high milk yield (p<0.01). Conclusion: The results of this study confirmed that cows on the animal welfare farm with a high percentage of grass feed had higher milk production efficiency with healthier rumen pH and blood metabolism parameters compared to those on the conventional farm.

Investigation on Bacillus anthracis isolated from Kyong-Ju (경주에서 분리된 탄저균에 대한 연구)

  • 이준규;이은미;차우양;김정화;김영환;이양수;김우현;정종식
    • Korean Journal of Veterinary Service
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    • v.18 no.1
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    • pp.41-56
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    • 1995
  • The present study was conducted to investigate results of B. anthracis isolated from Anthrax in the Kyong-Ju of Feb. 12. 1994. 1. In biochemical feature, B. anthracis was a gram-positive rod, non-motility, sporulation, capsulation. It was positive in gelatinase, starch hydrolysis, glucose. But negative in urease, arabinose, mannitol, xylose. 2. B. anthracis grew well on B4 Br A TSA after incubation for 24 hours. The organisim grew well on BA, Br. A, NA, TSA after incubation for 72 hours. The media grew well on Br A instead of BA. 3. On 5% blood agar by laboratory animal, ${\beta}$ -hemolysis was produced from 36 hours to 48 hours incubation. There was perfect ${\beta}$-hemolysis after incubation for 48 hours. On the other side ${\beta}$-hemolysis was begun on 5% goat blood agar after incubation for 60 hours. 4. In the test of antimicrobial susceptibility, B. anthracis was very sensitive to AM, CF, TE, ENR, GM, AN, DFX, S, P, TYLO, N, KM, C, E, Lins+Sp, NN, CC, CFP, CB were sensitive one by one. B. anthracis was no-sensitive to L, XNL, TIA, CL, SXT 5. B. anthracis had never sensitivity to direct inoculation of rat and chicken, after subcutanous inj. It was very sensitive to mouse and goat, hamster, guinea pig, rabbit had a sensibility one by one. 6. The dead laboratory animal which had been inoculated with B. anthracis preserved at $37^{\circ}C$ incubation, B. anthracis didn't cultivate on non-dissected animal after 80 hours but cultivate on dissected animal after 360 hours. 7. The rapidly death could cause high concentration, died from 420 after S. C. 8. The blood smeared samples of hamster from inoculation with B. anthracis, spore germinated In 37$^{\circ}C$ after 5 hours, in $32^{\circ}C$ after 6 hours, in room temperature after 9 hours, in $-4^{\circ}C$ to $-20^{\circ}C$ after 10 hours. 9. B, anthracis inoculated to laboratory animal after SC or PO. Mice and rats feces didn't cultivated with B. anthracis after SC, but did cultivated with B. anthracis after PO. 10. In the test of disinfectant, B. anthracis was high effective to $HgC1_2$, formalin, effect phenol, cresol, but non-effect NaOH, ethanol.

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A STUDY ON THE FOOD EFFECT OF NON-EDIBLE MARINE ALGAE(Part 1: Test on the Food Value for Chicken) (비식용해조의 사료효과 증진에 관한 연구(제1보, 유난의 사료가치 시험))

  • PARK Won Ki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.1 no.2
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    • pp.121-127
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    • 1968
  • This research has been dealt with the nutritional component analysis of two kinds of non-edible marine algae, Sargassum herneri (Turner) C. Ag. and Zostera marina Linne which grow abundantly around the southern coast of Korea, These marine algae were mixed in several kinds of samples rates with the combined feed for poultry sold in the market. These were given to 35 chicken in seven test divisions respectively. We have experimented with 35 chicken grown up for two weeks after hatchout. The combined food for poultry obtained from the market was set up as control divisions. The experiments were as follows: 1) The average weight increase in each test division during feeding (Table 5, Fig.2). 2) Food conversion rate and food efficiency in each test division (Table 7). 3) The comparison of digestive rate of crude protein during feeding (Table 8), The results were as follows: 1) The weight increasing rate of the test animal stock fed the food containing $5\~10\%$ of Sargassum horneri (Turner) C. Ag. powder was higher than the rate of those fed only market food for poultry. 2) The stock given food containing $10\%$ Zostera marine powder showed lower growth than the control divison. 3) No apparent trouble owing to salt component involved in the marine algae was found. 4) The stock given food containing sodium glutaminate and Sargassum horneri (Turner) C. Ag. had better result than that without sodium glutaminate.

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Comparative Analysis of the Responses to Intruders with Anxiety-Related Behaviors of Mouse

  • Kim, Sang-Hyeon;Kang, Eun-Chai;Park, Chan-Kyu
    • Animal cells and systems
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    • v.8 no.4
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    • pp.301-306
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    • 2004
  • Anxiety in mice can be measured by behavioral reactivity to social or non-social stressors. These behaviors were compared by performing the resident-intruder test (social) as well as the light-dark transition and open-field tests (non-social) for the FVB, C57BL/6, and BALB/c lines of mouse. The three inbred lines showed significant differences in their responses to intruder mice. Three factors, accounting for about 68% of the total variance, were extracted from the scores obtained from the three behavioral tests. The first two major factors are primarily associated with the anxiety-related behaviors. One includes anxiety behaviors with a locomotive basis, while the other includes defecation measured in both anxiety tests. The third factor explains the three social behaviors, facial investigation, ano-genital investigation, and following, observed in the resident intruder test, although facial investigation is also moderately associated with the second factor. The results indicate that the behavioral responses to an intruder share a component distinct from anxiety-related behaviors.

Properties of Enterococcus faecium isolated from the intestineof slaughting pigs (도축돈 장관에서 분리한 Enterococcus faeciurnab 특성)

  • Kim, In-doc;Ahn, Mi-hyun;Seok, Ho-bong
    • Korean Journal of Veterinary Research
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    • v.44 no.4
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    • pp.581-586
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    • 2004
  • The growth characteristics and the properties of the Enterococcus faecium (E. faecium) isolated from intestine of slaughting pig were examined in order to confirm whether it will be used practically as probiotics or not. E. faecium was identified by morphological and biochemical tests including of final confirming by API strep kit. Among 264 samples, 44 isolates (16.7%) of E. faecium were observed as an exellent growth as a range of $1{\times}10^7CFU/ml{\sim}3.3{\times}10^9CFU/ml$ in viable bacterial count on MRI medium. All isolates were shown non-haemolysis on the blood agar exception of 28 isolates shown ${\alpha}$-haemolysis and were identified as E. faecium by biochemical test using API strep enzyme kits. Eight strains (18.2%) were finally selected from which they were excellent sensitivity in showing of the acid-tolerance and the bile-tolerance in compare with reference strain.

Functional Analysis of Expressed Sequence Tags from Hanwoo (Korean Cattle) cDNA Libraries (한우 cDNA 라이브러리에서 발현된 ESTs의 기능분석)

  • Lim, Da-Jeong;Byun, Mi-Jeong;Cho, Yong-Min;Yoon, Du-Hak;Lee, Seung-Hwan;Shin, Youn-Hee;Im, Seok-Ki
    • Journal of Animal Science and Technology
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    • v.51 no.1
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    • pp.1-8
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    • 2009
  • We generated 57,598 expressed sequence tags (ESTs) from 3 cDNA libraries of Hanwooo (Korean Cattle), fat, loin, liver. Liver, intermuscular fat and longissimus dorsi tissues were obtained from a 24-month-old Hanwoo steer immediately after slaughter. cDNA library was constructed according to the oligocapped method. The EST data were clustered and assembled into unique sequences, 4,759 contigs and 7,587 singletons. To carry out functional analysis, Gene Ontology annotation and identification of significant leaf nodes were performed that were detected by searching significant p-values from $2^{nd}$ level GO terms to leaf nodes using Bonferroni correction. We found that 13, 26 and 8 significant leaf nodes are unique in the transcripts according to 3 GO categories, molecular function, biological process and cellular component. Also digital gene expression profiling using the Audic's test was performed and tissue specific genes were detected in the above 3 libraries.