• 대한화장품학회지
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• 제39권1호
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• pp.31-38
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• 2013

피부 자극과 부식 검사에 사용되는 동물실험을 대체하기 위한 방법으로 인공피부가 개발되어 왔다. 최근에 본 연구진은 녹각교를 함유하는 새로운 인공피부를 구축하였다. 현재 연구에서는 녹각교를 포함하는 인공피부를 사용하여 물질의 독성도 검사를 수행하였다. 그리하여 sodium dodecylsulfate (SDS) 또는 sodium carbonate를 인공피부에 도포하였고 표피의 손상 정도를 H&E와 면역조직화학 염색을 통하여 평가하였다. 인공피부의 표피는 SDS와 sodium carbonate에 의해 농도 의존적으로 영향을 받았다. 더 나아가 이들 물질에 의하여 p63의 발현이 감소하였다. 그러므로 녹각교를 함유하는 인공피부는 동물실험을 대체할 수 있는 모델로써 사용될 수 있고 in vitro에서 자극과 부식 검사 시험법의 발전에 도움을 줄 것으로 예상된다.

• 한국동물위생학회지
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• 제33권4호
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• pp.319-326
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• 2010

Bacteroiospermia is a frequently finding in fresh raw and extended porcine semen and can results in detrimental effects on semen quality and longevity. This study aims to evaluate the type of bacterial contaminants in raw and extended porcine semen and the reducing effect of antibiotic test. To investigate bacterial contaminants, out of 387 sample (raw semen 201, extended semen 186) were collected from 6 artifical insemination centers in Gyeongsangnam-do, were inoculated onto blood agar and MacKonkey agar, respectively. Bacterial colonies were selected after culturing for 48 hours, at $37^{\circ}C$, followed by Gram staining, KOH test, oxidase test, catalase test and eventually identified using VITEK System. Total 15 genus and 24 species of bacteria were isolated from these semen samlpes. In raw semen, the most prevalent contaminants were Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, Staphylococcus auricularis, Delftia acidovorans, Acinetobacter lowffii, S. aureus and others. And in extended porcine semen, A. lowffii, S. aureus, S. auricularis and other bacteria were identified. Most of them was G(-), which is nonpathogenic bacteria. It seems that bacterial contaminants in fresh raw and extended porcine semen originated from multiple sources at the farms/stud, and were from animal origin and non-animal origins. Whereas, the 7 virus which is known to be detected in porcine semen in 75 cases was not detected. This results showed that removal of bacterial contamination in raw and extended porcine semen is essential and farms were kept for biosecurity and individual hygienes.

• Asian-Australasian Journal of Animal Sciences
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• 제14권9호
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• pp.1228-1232
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• 2001

Forty five Soviet Chinchilla rabbits weaned at 35 d of age were fed 5 experimental diets prepared by using oat plant meal ($T_2,\;T_4$) and tall fescue hay ($T_3,\;T_5$) at 25% and 50% level along with control ($T_1$). The gain in body weight at 84 d of age decreased with test material substitution, however, the differences were non significant when compared with oat plant meal and were significant (p<0.05) with tall fescue hay at both the levels. Feed conversion ratio also deteriorated with test material and its increasing proportion and the differences were significant (p<0.05). The performance-index calculated were 42.1, 36.7, 29.4, 33.3 and 18.8 in $T_1,\;T_2,\;T_3,\;T_4$ and $T_5$, respectively. Digestibility of all the organic nutrients decreased significantly (p<0.05) with test material incorporation, as a result nutritive value of the test diets decreased linearly. Nitrogen balance as per cent of intake was higher in oat plant meal substituted diet compared to tall fescue substituted diet. Cost per kg live weight gain was lowest with 50% oat plant meal followed by 25% oat plant meal incorporation. It was highest with tall fescue hay substitution. Nutritive value of oat plant meal as calculated was DCP 5.1%, TDN 35.8% and that of tall fescue hay was DCP 6.5%, TDN 30.8%. Thus, oat plant meal can be substituted safely up to 25% in the feeding regime of weaner rabbits whereas, for tall fescue hay the safe limit is below 25% level and needs to be ascertained.

• 농업과학연구
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• 제45권3호
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• pp.411-418
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• 2018

A total of 576 seven-day-old male Ross 308 broilers with an average initial BW of $180{\pm}1g$ were used in a 4 week feeding experiment which included a starter phase (7 to 21 d) and a grower phase (22 to 35 d). Birds were randomly allocated into 1 of 3 treatments with 12 replicates per treatment and 16 birds per pen. The treatments consisted of the following: T1, Control; T2, T1 + 0.1% Creamer (Dongsuh Foods Corporation, Incheon, Korea), and T3, T1 + 0.5% Creamer. The broilers were weighed by pen and feed intake (FI) and the number of living broiler chickens were recorded on d 7, 21, and 35. These information were used to calculate the body weight gain (BWG) and feed conversion ratio (FCR). As results of this experiment, there were no significant differences in the BWG, FCR and nutrient digestibility among the treatments. With regards to meat quality, no adverse effects were observed among the treatments. However, a higher score in redness was observed in T3 than in T1. In addition, the relative weight of breast muscle was reduced in T3 compared with T1. Regardless of the nondairy creamer (NDC) inclusion levels, no negative effects on growth performance and nutrient digestibility were observed. In conclusion, non-dairy creamer could be a kind of fat sources additive in broiler diets, further studies are needed to test the optimum levels of the NDC to be supplemented in broilers diet.

• 한국가축번식학회지
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• 제19권4호
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• pp.259-264
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• 1996

This study was carried out to investigate on the survival rates and in vitro developmental rates of bisected bovine embryos were by manipulator and micropipette. Bisected embryos were co-cultured in 20% FCS(v/v)＋TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells 0 to 72 hrs after bisection. Survival rate and in vitro fertilization rate were defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro developmental rate of biseciton embryos co-cultured in 20% FCS＋TCM-199 medium containing PMSG, hCG, PMSG＋hCG, hCG＋$\beta$-estradiol 0 to 20 hrs and 20 to 40 hrs were 36.7, 26.7, 33.3, 40.0, and 30.0, and 30.0, 33.3, 30.0, 26.7, and 26.7%, respectively. The survival rate of bisection embryos co-cultured in TCM-199 medium containing hormones was significantly higher than that of non co-culture(25.0%). 2. The in vitro developmental rates of bisection embryos co-cultured in 20% FCS＋TCM-199 medium containing oviductal epithelial cells 4 to 5 hrs and 20 to 24 hrs were 40.0 and 33.3%, respectively. The survival rate of bisection embryos co-cultured in TCM-199 medium containing oviductal epithelial cells was significantly higher than that of non co-culture(25.0%). 3. The in vitro developmental rates of bisection embryos co-cultured in 20% FCS＋TCM-199 medium containing cumulus cells 4 to 5 hrs and 20 to 24 hrs were 43.3 and 36.7%, respectively. The survival rate of bisection embryos co-cultured in TCM-199 medium containing cumulus cells was significantly higher than that of non co-culture (25.0%).

• Preventive Nutrition and Food Science
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• 제18권4호
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• pp.292-295
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• 2013

Fermented whey solution presenting bifidogenic growth stimulation (BGS) activity was processed as prototypes such as sterilized fermented whey (SFW), spray-dried fermented whey (SDFW), and freeze-dried fermented whey (FDFW) and their BGS activities were compared. In optical density ($OD_{600}$) test, the BGS activity of three prototypes, which showed similar activities, were significantly different with non-fermented whey solution adjusted to pH 4.5 as a control (P<0.05). In viable cell count test, SDFW had the most positive influence than other prototypes on the BGS activity even though the difference was not significant. However, the activities of all prototypes were significantly different than the negative control (no addition). These results indicate that the processed prototypes of fermented whey solution show BGS activities and might be commercialized, with further evidences, in animal or human studies.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1121-1127
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• 2002

Sixteen crossbred (Bos indicus${\times}$Bos taurus) calves were randomly distributed in two groups (NP and LP) of eight calves each to study the effect of restricted (75%) protein supply on growth and immuno-biochemical response as an indicator of production and health of under-nourished animals during 3 to 9 months of age. The normal requirement of protein was provided to group NP and a less of 25% to group LP through calculated amount of concentrate and roughage in their daily ration. Assessment was made for weekly change in live weight, periodic alteration in blood metabolites and immunological status at six months of age in calves. An initial (during 3 to 6 months of age) depression (p<0.05) in growth was seen in low protein fed group (LP) compared to NP, which became non-significant in the later period of life (6 to 9 months of age). There was no significant effect on haemoglobin, total protein, albumin and globulin concentration except that of urea, which was decreased significantly (p<0.05) in animals fed on low protein diet ($19.83{\pm}1.25$ vs $25.93{\pm}1.29mg/dl$). The treatment effect that was seen in different periods of life was not uniform for other parameters except for urea, which showed a regular depression in LP compared to NP. The assessment of immunological status by indirect haemagglutination (IHA) test against Pasteurella multocida (P52 strain) was considerably (p<0.05) reduced in animals on LP ration compared to those on NP. It is thus argued that with poor nutrition (low protein) and state of compromised immunological response the production and health of the animals will be adversely affected.

• Journal of Veterinary Science
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• 제21권2호
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• pp.22.1-22.9
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• 2020

Rabid raccoon dogs (Nyctereutes procyonoides koreensis) have been responsible for animal rabies in South Korea since the 1990s. A recombinant rabies vaccine strain, designated as ERAGS, was constructed for use as a bait vaccine. Therefore, new means of differentiating ERAGS from other rabies virus (RABV) strains will be required in biological manufacturing and diagnostic service centers. In this study, we designed two specific primer sets for differentiation between ERAGS and other RABVs based on mutation in the RABV glycoprotein gene. Polymerase chain reaction analysis of the glycoprotein gene revealed two DNA bands of 383 bp and 583 bp in the ERAGS strain but a single DNA band of 383 bp in the field strains. The detection limits of multiplex reverse transcription polymerase chain reaction (RT-PCR) were 80 and 8 FAID₅₀/reaction for the ERAGS and Evelyn-Rokitnicki-Abelseth strains, respectively. No cross-reactions were detected in the non-RABV reference viruses, including canine distemper virus, parvovirus, canine adenovirus type 1 and 2, and parainfluenza virus. The results of multiplex RT-PCR were 100% consistent with those of the fluorescent antibody test. Therefore, one-step multiplex RT-PCR is likely useful for differentiation between RABVs with and those without mutation at position 333 of the RABV glycoprotein gene.

• Journal of Animal Science and Technology
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• 제65권5호
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• pp.1094-1104
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• 2023

Aggression in horses may cause serious accidents during riding and non-riding activities. Hence, predicting the temperament of horses is essential for selecting suitable horses and ensuring safety during the activity. In certain animals, such as hamsters, plasma melatonin concentrations have been correlated with aggressive behavior. However, whether this relationship applies to horses remains unclear. To address this research gap, this study aimed to evaluate differences in the plasma melatonin concentrations among horses of different breeds, ages, and sexes and examine the correlation between plasma melatonin concentrations and the temperament of the horses, including docility, affinity, dominance, and trainability. Blood samples from 32 horses were collected from the Horse Industry Complex Center of Jeonju Kijeon College. The docility, affinity, dominance, and trainability of the horses were assessed by three professional trainers who were well-acquainted with the horses. Plasma melatonin concentrations were measured using an enzyme-linked immunosorbent assay. The consequent values were compared between the horses of different breeds, ages, and sexes using a three-way analysis of variance and least significant difference post hoc test. Linear regression analysis was employed to identify the relationship between plasma melatonin concentrations and docility, affinity, dominance, and trainability. The results showed that the plasma melatonin concentrations significantly differed with breeds in Thoroughbred and cold-blooded horses. However, there were no differences in the plasma melatonin concentrations between the horse ages and sexes. Furthermore, plasma melatonin concentrations did not exhibit a significant correlation with the ranking of docility, affinity, dominance, and trainability.

• 대한화장품학회지
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• 제40권2호
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• pp.141-153
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• 2014

본 연구는 화장품 제형 적용성이 높은 콜레스테릭 액정(CLCs) 개발에 관한 것이다. 비동물성 원료는 동물성 바이러스 감염 이슈로부터 자유롭고 화장품 소비자들에게 안심감을 줄 수 있기 때문에 비동물성 유래로부터 콜레스테릭 액정연구를 진행하였다. 따라서 발효로부터 만들어진 비동물성 콜레스테롤[NAB cholesterol (ARCH LONZA)]을 이용하여 3 가지 새로운 콜레스테릭 유도체(CI, CC, CN)를 합성하였다. 그리고 제형 적용성이 높은 콜레스테릭 액정개발을 위해서 넓은 온도범위에서 발색을 유지하는 최적 조성연구를 진행하였다. 콜레스테릭 액정은 3 가지 유도체를 조합하여 41개의 액정을 제조하고 온도에 따른 육안평가를 통해서 색을 나타내는 범위를 평가하였다. 제조된 41개의 액정을 온도에 따라 UV-VIS 스펙트럼을 동시에 분석할 수 있는 새로운 평가법을 사용하였으며 205개의 스펙트럼 분석하고 액정에 영향을 미치는 인자와 특성을 파악하였다. 위와 같은 결과를 토대로 제형적용성이 높은 비동물성 콜레스테릭 액정 A4, A5, A6, A7의 조성을 개발하였다. 또한 인공피부를 이용한 평가를 통하여 A6 조성의 뛰어난 피부 보습효능을 확인하였다.