• 대한한방내과학회지
• /
• 제26권4호
• /
• pp.853-863
• /
• 2005

Object : This study was performed to investigate the anti-fibrogenic effect of Artemisiae Capillaris Herba(ACH) on cultured rat hepatic stellate cells. Methods : Hepatic Stellate Cells were obtained from a 350gm Sprague-Dawley rat by tissue perfusion system, and the cells for the study were selected after 3 passages of culture on non-coated plastic culture dishes which enable the cells to activate, thus producing collagens in the cell media. Cells were treated with various concentrations of Artemisia Capillaris Herba(ACH) extract powder for 24 or 48 hours. After the treatment, Soluble collagen, procollagen levels and the mRNA of the procollagen type I C were measured by using assay kit and RT-PCR method. Results : Procollagen production by the hepatic stellate cells decreased after the treatment in a time-dependent dose-dependent manner. The mRNA expression decreased consistently with the volume of the secreted procollagen which indicates the herb hat inhibitory effect on fibrogenesis of the liver by regulating one of the fibrosis associated genes in transcription. Conclusion : These results suggest that ACH is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제10권4호
• /
• pp.309-315
• /
• 2010

A fully-differential low-voltage low-power electrocardiogram (ECG) amplifier by using the nonfeedback PMOS pseudo-resistors is proposed. It consists of two operational-transconductance amplifiers (OTA) in series (a preamplifier and a variable-gain amplifier). To make it insensitive to the gate leakage current of the OTA input transistor, the feedback pseudo-resistor of the conventional ECG amplifier is moved to input branch between the OP amp summing node and the DC reference voltage. Also, an OTA circuit with a Gm boosting block without reducing the output resistance (Ro) is proposed to maximize the OTA DC gain. The measurements shows the frequency bandwidth from 7 Hz to 480 Hz, the midband gain programmable from 48.7 dB to 59.5 dB, the total harmonic distortion (THD) less than 1.21% with a full voltage swing, and the power consumption of 233 nW in a 0.13 ${\mu}m$ CMOS process at the supply voltage of 0.7 V.

• BMB Reports
• /
• 제38권6호
• /
• pp.717-724
• /
• 2005

The nontoxic B subunit of cholera toxin (CTB) can significantly increase the ability of proteins to induce immunological tolerance after oral administration, when it was conjugated to various proteins. Recombinant CTB offers great potential for treatment of autoimmune disease. Here we firstly investigated the feasibility of silkworm baculovirus expression vector system for the cost-effective production of CTB under the control of a strong polyhedrin promoter. Higher expression was achieved via introducing the partial non-coding and coding sequences (ATAAAT and ATGCCGAAT) of polyhedrin to the 5' end of the native CTB gene, with the maximal accumulation being approximately 54.4 mg/L of hemolymph. The silkworm bioreactor produced this protein vaccine as the glycoslated pentameric form, which retained the GM1-ganglioside binding affinity and the native antigenicity of CTB. Further studies revealed that mixing with silkworm-derived CTB increases the tolerogenic potential of insulin. In the nonconjugated form, an insulin : CTB ratio of 100 : 1 was optimal for the prominent reduction in pancreatic islet inflammation. The data presented here demonstrate that the silkworm bioreactor is an ideal production and delivery system for an oral protein vaccine designed to develop immunological tolerance against autoimmune diabetes and CTB functions as an effective mucosal adjuvant for oral tolerance induction.

• Journal of the Korean Society for Industrial and Applied Mathematics
• /
• 제18권3호
• /
• pp.257-268
• /
• 2014

The objective of the present study is to investigate thermal diffusion and radiation effects on unsteady MHD flow past a linearly accelerated vertical porous plate with variable temperature and also with variable mass diffusion in presence of heat source or sink under the influence of applied transverse magnetic field. The fluid considered here is a gray, absorbing/emitting radiation but a non-scattering medium. At time t > 0, the plate is linearly accelerated with a velocity $u=u_0t$ in its own plane. And at the same time, plate temperature and concentration levels near the plate raised linearly with time t. The dimensionless governing equations involved in the present analysis are solved using the closed analytical method. The velocity, temperature, concentration, skin-friction, the rate or heat transfer and the rate of mass transfer are studied through graphs in terms of different physical parameters like magnetic field parameter (M), radiation parameter (R), Schmidt parameter (Sc), Soret number (So), Heat source parameter (S), Prandtl number (Pr), thermal Grashof number (Gr), mass Grashof number (Gm) and time (t).

• 대한한방내과학회지
• /
• 제1권1호
• /
• pp.70-77
• /
• 1976

The Sam Mool Injin extract is occasionally used as a kind of remedies for the treatment of hepatic discomfort in herb medicine. The study for the effect on the hepatic parenchymal cells, especially regenerating activity and even its toxicity have. little been done and only a very few pharmacological properties have been reported. So, an experimental study for the morphologic changes in the acute carbon tetrachloride poisoning of wister rat by administration of its extract has been performed. Male Wister rat weighing around 200gm were used in this experiment. Results were as follows; 1) In the $Ccl_4$ 0.3c.c 7day group, there is noted considerable increase of regenerating activity in the experimental group compared with non-extract treated group, in half of them. 2) Remaining all of the experimental group do not show significant difference of regenerating activity compared with control group. 3) In the group treated with Sam Mool Injin extract alone, there was noted some considerable increase of nonspecific leukocytic infiltrations compared with saline control group. This can be considered as a possibility of hepatic tissue reaction on the Sam Mool Injin extract.

• 한국수학교육학회지시리즈A:수학교육
• /
• 제55권1호
• /
• pp.73-89
• /
• 2016

The purpose of this study was to analyze middle school students' problem posing types and strategies. we analyzed problems posed by 120 middle school students during mathematics class focused on problem posing activities in various aspects. Students' posed problems were classified into five types: not a problem(NP), non-math(NM), impossible(IM), insufficient(IN), sufficient(SU) and each of the posed problems. Students used three kinds of problem posing strategies such as goal manipulation(GM), assumption manipulation(AM), and condition manipulation(CM), and in posing one problem, one or more than two strategies were used. According to the prior studies, problem posing can contributes to the development of students' problem solving ability, creativity, mathematical aptitude, and a broader understanding of mathematical concepts. However, we found that some students had difficulties in posing problems or limited understandings of that. We hope the results of the study contribute to encouraging problem posing activities in mathematics instruction.

• Clinics in Shoulder and Elbow
• /
• 제25권3호
• /
• pp.240-243
• /
• 2022

Isolated deltoid paralysis is a rare pathology that can occur after axillary nerve injury due to shoulder trauma or infection. This condition leads to loss of deltoid function that can cause glenohumeral instability and inferior subluxation, resulting in rotator cuff muscle fatigue and pain. To establish dynamic glenohumeral stability, a novel technique was invented. Humeral suspension is achieved using a double button implant with non-resorbable high strength cords between the acromion and humeral head. This novel technique was used in two patients with isolated deltoid paralysis due to axillary nerve injury. The results indicate that the humeral suspension technique is a method that supports centralizing the humeral head and simultaneously dynamically stabilizes the glenohumeral joint. This approach yielded high patient satisfaction and reduced pain. Glenohumeral alignment was improved and remained intact 5 years postoperative. The humeral suspension technique is a promising surgical method for subluxated glenohumeral joint instability due to isolated deltoid paralysis.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권9호
• /
• pp.4039-4044
• /
• 2014

Background: The aim of this study was to investigate the anti-cancer effects and mechanisms of immunochemotherapy of 5-fluorouracil (5-FU) and interleukin-2 (IL-2) on non-small cell lung cancer (NSCLC) A549 cells. Materials and Methods: In order to detect whether 5-FU+IL-2 could effectively inhibit tumor growth in vivo, we established an A549-bearing nude mouse model. The cytotoxicity of natural killer (NK) cells was evaluated using a standard chromium release assay. To evaluate the relevance of NK cells in 5-FU+IL-2-mediated tumor inhibitory effects, we depleted NK cells in A549-bearing mice by injecting anti-asialo-GM-1 antibodies. Effects of 5-FU+IL-2 on the expression and promoter activity of NKG2D ligands (MICA/MICB) in A549 cells in vitro were also assessed. Results: In A549-bearing nude mice, combination therapy significantly inhibited tumor growth in comparison with monotherapy with 5-FU or IL-2 and enhanced the recognition and lysis of tumor cells by NK cells. Further study of mechanisms showed that NK cells played a vital role in the anticancer immune response of 5-FU+IL-2 immunochemotherapy. In addition, the combination therapy synergistically stimulated the expression and promoter activity of MICA/MICB. Conclusions: 5-FU and IL-2 immunochemotherapy significantly inhibited tumor growth and activated NK cytotoxicity in vivo, and these effects were partly impaired after depleting NK cells in tumor-bearing mice. Combination treatment of 5-FU and IL-2 upregulated the expression and the promoter activity of MICA/MICB in A549 cells, which enhanced the recognition of A549 cells by NK cells. All of the data indicated that immunochemotherapy of 5-FU and IL-2 may provide a new treatment option for patients with lung cancer.

• Journal of Chest Surgery
• /
• 제24권6호
• /
• pp.570-578
• /
• 1991

The spontaneous pneumothorax is the sudden collapse of the lung usually by air leakage from the ruptured sub-pleural bleb and has high recurrence rate. For prevention against recurrence, many drugs such as tetracycline, talcum powder, quinacrine, etc. have been used but the effects are not satisfactory. We reduced the recurrence rate successfully by the fibrin glue instillation through the chest tube. From the January 1989 to September 1990, we have managed 65 patients of spontaneous pneumothorax with closed thoracostomy and fibrin glue[fibrinogen 1gm/50ml with approtinin 3, 000kIU /ml, thrombin 5, 000IU /ml in 3% each 10ml] instillation through the chest tube. And we compared the results with those of 106 patients of spontaneous pneumothorax who were managed only by the closed thoracostomy from January 1985 to December 1988. Only the patients who visited our hospital with recurrence were considered as the recurred cases but the others were considered as not recurred. And the removal of chest tubes usually done 3 days after cessation of air leakage or 2 days after fibrin glue instillation Statistical analysis was done by X2-test. The results were as followings: 1. The recurrence rate of fibrin glue instillation group was lower than that of non-instillation group[1st attack: 15.1% versus 27.6% p<0, 05, the 2nd attack: 33.3% versus 73.7% p<0.01, the total 18.5% versus 35.8% p<0.01]. 2. The mean duration of chest tube drainage in the fibrin glue instillation group was shorter than non-instillation group[4.24$\pm$1.36 days versus 4.48$\pm$1.73 days p<0.05]. 3. The mean duration of hospitalization was shorter in the instillation group [8.12$\pm$3.5 days versus 10.8$\pm$3.8 days p<0.05] The complications were transient mild fever, chest pain, pleural effusion in 46 cases of 65 patients, but those didn`t make any problem. We concluded that the fibrin glue is effective in the reduction of recurrence rate, obliteration of air leakage and duration of hospitalization.

• 수산해양기술연구
• /
• 제48권3호
• /
• pp.208-216
• /
• 2012

The properties of sediment collected from seabed surface to 6cm depth on the four positions were analyzed to investigate turbulence of marine sediments by shrimp beam trawl. Types of sediments in the investigation area were (g)mS (slightly gravely muddy sand) and gmS (gravely muddy sand) showing high sand content, and (g)sM (slightly gravely sandy mud), gsM (gravely sandy mud) as well. It is estimated that position is more crucial factor than seasonal difference for the granularity variation of sediment in each investigation area. Finding the positional characteristics of sediment granularity was difficult before removing shells and organic matter. However, the average granularity is getting larger by going out from inland sea to open sea once those were removed. The granularity of marine sediment got narrow after processing in the fishing area for shrimp beam trawl but there was no big difference for granularity size before and after processing in the non-fishing area. This might be attributed to crushed shell particles going up and down again on the surface in the fishing area. To demonstrate the hypothesis mentioned above, the sediments driven by shrimp beam trawl need to be collected and analyzed.