• Journal of Life Science
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• v.28 no.10
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• pp.1156-1162
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• 2018

To investigate the anti-thrombotic activities of the lotus (Nelumbo nucifera Gaertner), various hot-water extracts were prepared from the leaf, pod of seed (PS), seed, embryo of seed (ES), root, and the node of root (NR) of the lotus. The highest extraction ratio was found in the NR (20.3%), followed by the seed, root, leaf, ES, and PS. These extracts had pH and acidity levels ranging from 5.6~6.5 and 0.06~0.20%, respectively. The seed extract showed 70% brix, whereas the leaf and PS extracts showed less than 0.1% brix. The highest contents of total polyphenol (179.7 mg/g), total flavonoids (161.4 mg/g), and reducing sugar (161.4 mg/g) were observed in the leaf extract, and the highest total sugar content (873.0 mg/g) in the seed extract. Anti-coagulation assay of the extracts of NR, leaf, and PS showed strong activities. In particular, at a concentration of 5 mg/ml, the PS extract had 15-fold extended thrombin, prothrombin, and activated partial thromboplastin times. However, only the ES extract showed activities inhibitory to platelet aggregation, with treatment with 0.25 mg/ml of ES extract decreasing platelet aggregation to 25.1%, a reduction comparable to that effected by aspirin. The extracts other than the seed extract showed no hemolysis activities against human RBC at treatments of up to 1 mg/ml. These results suggest that the NR, PS, seed, and ES, all byproducts of the lotus agriculture industry, have high potential as novel sources of anti-thrombotic agent.

• Journal of Bio-Environment Control
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• v.29 no.1
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• pp.1-8
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• 2020

The influence of growth regulators (NAA and BA) and sucrose concentrations (0, 3, 5, 7, 9%) on in vitro rapid-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] was investigated with single-node or shoot-tip culture of two cultivars ('Matnami' and 'Shinhwangmi'). The survival rate and growth of shoot-tip explant was also investigated under the presence or absence of light (blue and red LED = 7:3, 150±5 μmol·m^-2·s^-1 PPFD) during minimal-growth in vitro conservation at 15℃. Vine length, vine diameter, fresh weight and dry weight were enhanced without callusing of explant in the MS medium supplemented with 0.2-0.5 mg·L^-1 BA. The growth of single-node and shoot-tip explants were significantly enhanced with the increase of vine length, number of leaf, number of root, fresh weight, and dry weight in the solid medium containing 5% sucrose and 0.2 mg·L^-1 BA. Vine elongation of shoot-tip explants were highest in the liquid medium containing 3% sucrose than the solid medium. The survival rate of minimal-growth in vitro conservation was 100% in 5 months under the presence of light (LED, 150±5 μmol·m^-2·s^-1 PPFD) at 15℃, but the explants in dark condition died in 3 months. The light was absolutely necessary for the in vitro conservation under minimal-growth conditions of virus-free sweet potato plantlets at 15℃, and the high density of explants (10 plantlets per Petri Dish) was increased the efficiency of mass conservation.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.686-693
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• 2012

To develop a technique for mass-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plant using nutrient film technique (NFT), the growth characteristics of 4 cultivars as affected by nutrient solution composition and cutting size were investigated. 72 cells (35 mL/cell) plug trays filled with vermiculite and perlite (1:1, v/v) were used. Vine length, fresh and dry weights of virus-free plants were the greatest in the nutrient solution recommended by National Horticultural Research Station in Japan, followed by that recommended by National Institute of Horticultural & Herbal Science in Korea, and Yamazaki's nutrient solution for lettuce. The growth of uppershoot cuttings was the best among 4 subsections of cutting. Vine length, and fresh and dry weights increased in the longer cutting treatments, and were better in 'Shinzami' and 'Yeonhwangmi' than those in 'Mannami' and 'Shincheonmi'. Vine diameter and length of the longest root were not significantly affected by the cutting size and cutting source. The growth characteristics of the single node cutting were not significantly different from those in 2-node cutting. The efficiency of rapid mass-propagation could be promoted with single node cuttings and uppershoot cuttings grown in NFT system.

• Advances in Computational Design
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• v.5 no.3
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• pp.305-321
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• 2020

In this paper is presented the solution method for three-dimensional problem of transversely isotropic body's elastoplastic deformation by the finite element method (FEM). The process of problem solution consists of: determining the effective parameters of a transversely isotropic medium; construction of the finite element mesh of the body configuration, including the determination of the local minimum value of the tape width of non-zero coefficients of equation systems by using of front method; constructing of the stiffness matrix coefficients and load vector node components of the equation for an individual finite element's state according to the theory of small elastoplastic deformations for a transversely isotropic medium; the formation of a resolving symmetric-tape system of equations by summing of all state equations coefficients summing of all finite elements; solution of the system of symmetric-tape equations systems by means of the square root method; calculation of the body's elastoplastic stress-strain state by performing the iterative process of the initial stress method. For each problem solution stage, effective computational algorithms have been developed that reduce computational operations number by modifying existing solution methods and taking into account the matrix coefficients structure. As an example it is given, the problem solution of fibrous composite straining in the form of a rectangle with a system of circular holes.

• 전기의세계
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• v.18 no.6
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• pp.9-22
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• 1969

This thesis has suggested a method of applying the Maximum Principle of Pontryagin to the optimal control of distributive electrical networks. In general, electrical networks consist of branches, nodes, sources and loads. The effective values of steady state currents and voltages are independent of time but only expressed as the functions of position. Moreover, most of the node voltages and branch currents are not predetermined, that is, initially unknown, and their inherent loop characteristics satisfy only Kirchhoff's current and voltage laws. The Maximum Principle, however, needs the initial fixed values of all state variables for its standand way of application. In spite of this inconsistency this thesis has undertaken to suggest a new approach to the successful solution of the above mentioned networks by introducing scaling factors and a state variable change technique which transform the boundary-value unknown problem into the boundary-value partially fixed and partially free problem. For the examples of applying the method suggested, the control problems for minimizing copper quantity in a distribution line have been solved with voltage drop constraint imposed on. In the case of uniform load distribution it has been shown that the optimal wire diameter of the distribution line is reciprocally proportional to the root of distance. For the same load pattern as above the wire diameter giving the minimum copper loss in the distribution line has been shown to be reciprocally proportional to distance.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.30-30
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• 2019

Pea (Pisum sativum) is one of important legume crops in the world. It is commonly used as a protein source for animal and human diet, and also used as a natural nitrogen source which is produced by a symbiotic bacterium in their root nodule and helpful for terrestrial ecosystem. The successful in vitro manipulation is depended on three main factors including physiology of plant donor, in vitro manipulation approach, and stress physiology during plant cultivation. Moreover, genotype is an important for plant manipulation; different genotype gives the different response to regeneration efficiency. An efficient condition of shoot induction for pea (Pisum sativum cv. 'Sparkle') was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 mg/L BA and a combination of 2 mg/L BA and 1 mg/L TDZ) with cotyledonary node explants culture. Moreover, the pretreatment of explant in 200 mg/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. The analysis of genetic stability of regenerants by using 13 ISSR markers presented that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.69-75
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• 2004

Optimal protocol for efficient genetic transformation has been defined to aid future strategies of genetic engineering in pigeon pea with agronomically important genes. Transgenic pigeonpea plants were successfully produced through Agrobacterium tumefaciens-mediated genetic transformation method using cotyledonary node explants by employing defined culture media. The explants were co-cultivated with A. tumefaciens strain C-58 harboring the binary plasmid, pCAMBIA-1301 [con-ferring $\beta$-glucuronidase(GUS) activity and resistance to hygromycin] and cultured on selection medium (regeneration medium supplemented with hygromycin) to select putatively transformed shoots. The shoots were then rooted on root induction medium and transferred to pots containing sand and soil mixture in the ratio of 1:1. About 22 putative TO transgenic plants have been produced. Stable expression and integration of the transgenes in the putative transgenics were confirmed by GUS assay, PCR and Southern blot hybridization with a transformation efficiency of over 45%. Stable integration and expression of the marker gene has been confirmed in the TO and T1 transgenics through PCR, and Southern hybridization.

• ETRI Journal
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• v.38 no.1
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• pp.18-29
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• 2016

In this paper, we discuss the issues of providing protection for point-to-multipoint connections in both Ethernet and MPLS-TP-based packet transport networks. We introduce two types of per-leaf protection-linear and ring. Neither of the two types requires that modifications to existing standards be made. Their performances can be improved by a collective signal fail mechanism proposed in this paper. In addition, two schemes - tree protection and hybrid protection - are newly proposed to reduce the service recovery time when a single failure leads to multiple signal fail events, which in turn places a significant amount of processing burden upon a root node. The behavior of the tree protection protocol is designed with minimal modifications to existing standards. The hybrid protection scheme is devised to maximize the benefits of per-leaf protection and tree protection. To observe how well each scheme achieves an efficient traffic recovery, we evaluate their performances using a test bed as well as computer simulation based on the formulae found in this paper.

• Journal of Biosystems Engineering
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• v.43 no.4
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• pp.352-361
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• 2018

Purpose: The objective of this study was to develop a multilayer perceptron (MLP) model to estimate solar radiation using a solar module. Methods: Data for the short-circuit current of a solar module and other environmental parameters were collected for a year. For MLP learning, 14,400 combinations of input variables, learning rates, activation functions, numbers of layers, and numbers of neurons were trained. The best MLP model employed the batch backpropagation algorithm with all input variables and two hidden layers. Results: The root-mean-squared error (RMSE) of each learning cycle and its average over three repetitions were calculated. The average RMSE of the best artificial neural network model was $48.13W{\cdot}m^{-2}$. This result was better than that obtained for the regression model, for which the RMSE was $66.67W{\cdot}m^{-2}$. Conclusions: It is possible to utilize a solar module as a power source and a sensor to measure solar radiation for an agricultural sensor node.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.135-141
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• 2005

Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with $0.54\;{\mu}M$ naphthalene acetic acid (NAA) and $8.87\;{\mu}M$ benzy-ladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum. For in vitro conservation studies, experiments were carried out with 2-3 week maintained in vitro plants under standard and reduced culture conditions (SCC, RCC). In vitro plants could be successfully conserved in full strength MS medium (FMS) under SCC for 6 months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature (15 and $10^{\circ}C$) and reduction in media constituents does not appear to favour conservation, although the former accomplished conservation levels equal to (FMS) under SCC.