• Journal of fish pathology
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• v.22 no.1
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• pp.85-90
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• 2009

A method for the identification of Nocardia seriolae, the causative agent of nocardiosis in cultured fishes, using PCR was developed in the study. A PCR primer set specific to N. seriolae was designed based on 16S-23S rRNA sequence of various Nocardia species accessed in GenBank. Designed PCR primer set, Nseri-F (5'-GCA AAC TCT TCG AAC AGT CG-3') and Nseri-R (5'-GGA TAT CAG GAC TTA CCG GC-3'), amplifies the target regions of N. seriolae only, but not 4 other Nocardia species, N. asteroides, N. crassostreae, N. farcinica and N. salmonicida.

• Journal of Environmental Science International
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• v.11 no.6
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• pp.561-567
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• 2002

This study was investigated to evaluate the effect of the sodium ion and pH on toxicity of dinitrophenol at high concentrations (0.41 to 0.54 mM), over a sodium concentration range of 0.1 mM to 107 mM and over a pH range of 5 to 9. The concentration of sodium ions in the activated sludge mixed liquor seemed to have very little effect on dinitrophenol toxicity. However, lack of sodium in the growth media resulted in a reduction of the dinitrophenol degradation rate by bacterial isolate from the activated sludge culture, which has been identified as Nocardia asteroides. Dinitrophenol inhibition was found to be strongly dependent on mixed liquor pH. The dinitrophenol degradation rate was highest in the pH range of 6.95 to 7.84; at pH 5.94 degradation of 75 mg/L dinitrophenol was significantly inhibited; at pH < 5.77, dinitrophenol degradation was completely inhibited after approximately 30% of the dinitrophenol was degraded.

• Tuberculosis and Respiratory Diseases
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• v.39 no.4
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• pp.355-360
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• 1992

Nocardiosis is an acute, subacute or chronic infection, which usually introduced through the respiratory tract resulting pneumonia, and may develop a disseminated infection, especially subcutaneous abscess and/or CNS infection. It is usually affects the immunocompromized host and may be fatal unless early diagnosis and adequate treatment are performed. There have been only several case reports of nocardial infection in Korea. Nocardiosis is so unfamiliar to many physicians that may be misdiagnosed as pneumonia, tuberculosis, or neoplasm. We have experienced a case of nocardiosis from a patient who had been treated as pneumonia and tuberculosis at first. The 57-year-old male patient had fever, chill, dyspnea and blood tinged purulent sputum for 20 days. Under the impression of bacterial pneumonia, broad spectrum antibiotics were administered for more than 3 weeks without clinical improvement. Although antituberculous drugs began to be administered after acid fast bacilli were found in bronchial aspirate by bronchoscopy, the nocardial infection was suspected due to no clinical response toward antituberculous therapy and the occurrence of multiple subcutaneous abscesses on scalp. The diagnosis was made by modified Ziehl-Neelson stain and culture of the sputum and pus. Nocardia asteroides was identified. After 25 days of trimethoprim-sulfamethoxazole treatment, the patient was much improved and discharged.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.3 no.3
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• pp.169-175
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• 1999

2,4-Dinitrophenol(DNP) is a metabolic uncoupler that prevents cells from creating energy for growth and it has been suggested that the availability of sodium ions may be important in mitigating the effects of uncouplers. Accordingly, the degradation of DNP was investigated using activated sludge which had been adapted to mineralize DNP. After the acclimation of the activated sludge, the effect of sodium ions on the toxicity of high concentrations(80 to 100mg/L) of DNP was investigated over a sodium ion concentration range of 9.3$\times$10-5 to 94mM. The concentration of sodium ions in the activated sludge mixed liquor seemed to have little effect on the DNP toxicity. However, a lack of sodium in the grwoth media resulted in a reduction of the DNP degradation rate by a bacterial isolate from the activated sludge culture identified as Nocardia asteroides.

• Investigative Magnetic Resonance Imaging
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• v.17 no.1
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• pp.50-54
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• 2013

Nocardiosis is an uncommon Gram-positive bacterial infection caused by aerobic actinomycetes in the genus Nocardia. Nocardia spp. have the ability to cause localized or systemic suppurative disease in humans and animals. Nocardiosis is typically regarded as an opportunistic infection, but approximately one-third of infected patients are immunocompetent. We report a rare case of pulmonary nocardiosis and a brain abscess caused by Nocardia asteroides in an elderly woman with a history of Crohn's disease. Radiographic imaging revealed a contrast-enhancing lesion with perilesional parenchymal edema that was preoperatively thought to be a neoplasm. The patient experienced aggressive disease progression simulating a metastatic brain tumor. Early diagnosis of norcadiosis, the absence of underlying disease, and the administration of appropriate antibiotics has a positive impact on prognosis. Familiarity with the magnetic resonance and computed tomography findings associated with CNS nocardiosis, such as those presented here, is essential for making an early diagnosis.

• The Journal of the Korean Society for Microbiology
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• v.10 no.1
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• pp.25-32
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• 1975

The majority of Nocardial infections reported in North America areIN. ;asteroides while those in Latin America are N. brasiliensis species. Infection with N. brasiliensis, to our knowledge, has not previously been reported in Korea. The auther isolated one strain of Nocardia brasiliensis from the abscess of right bottock of 23 month old female leukemic patient who was treated with methotrixate for five months at the Seoul National University Hospital. The morphological characteristics and biological properties were similar with the R.E. Gordon's description. The results are summarized as follow: 1. After 5 days incubation on Sabouroud's glucose agar, the acid fast character appeared partially. 2. Tyrosine, casein and urea were decomposed by 7 days incubation both at room temperature and at $37^{\circ}C$. 3. Sod, citrate and sod. acetate were utilized at $22^{\circ}C\;and\;37^{\circ}C$ after 28 days incubation while the sod. benzoate utilization was negative. 4. The survival range of temperature was from $10^{\circ}C\;to\;40^{\circ}C$. 5. Dulcitol, galactose, glycerol, lactose, maltose, mannitol, raffinose, rhamnose, sorbitol, trehalose and xylose fermentations were not observed up to 28 days, while the fermentation of glucose and inositol were positive.