• Environmental Analysis Health and Toxicology
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• v.15 no.4
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• pp.131-137
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• 2000

The release of hazardous waste materials into the environment poses serious risks in humans and ecosystems. The risk assessment of environmental pollutants including hazardous chemicals requires a comprehensive measurement of hazard and exposure of the chemicals that can be achieved by toxicity evaluation using a biological system such as biomarkers. In this report we have tried to develop a biomarker used to elucidate a molecular basis of, and to monitor abnormal behaviors caused by diazinon in Japanese medaka (Oryzias latipes) as a model organism. First, an attempt was made to clone tyrosine hydroxylase gene from Japanese medaka that would be a candidate for a biomarker for neuronal modulations and behaviors. For monitoring experiments at behavioral and molecular biological levels, the fish were treated under different sublethal conditions of diazinon and their behavioral responses were observed . In this study we have successfully cloned a partial TH gene from the medaka fish through PCR screening of an ovary cDNA library. DNA sequencing analysis revealed that the amplified fragment was 327 bp encoding 109 amino acids. Comparing the DNA sequence of medaka TH with other species, TH gene revealed the DNA sequence was completely identical to that of rat TH. In the RT-PCR, 330 Up of mRNA was consistently amplified in all the treated samples including control There were no significant differences in the TH expression level regardless of treating concentrations (1∼5,000 ppb) and time (0∼48 hr) The reason appeared to be that RT-PCR was not performed using through a quantitative analysis normalized against an actin gene expression. Organ or tissue - specific detection of TH activity and mRNA as biomarkers will be a useful monitoring tool for neurobehavioral changes in fish influenced by toxic chemicals. Furthermore, quantitative analysis of locomotive patterns and its correlation with the neurochemical and molecular data would be highly useful in measuring toxicity and hazard ofvarious environmental pollutants.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.67-78
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• 2007

This study was carried out to monitor feed-nutritional components, toxic heavy metals (Cd, Pb and As) and pesticide residues through three cultivation stages (1st initial culture stage, 2nd mycelial growth stage, and 3rd fruit body-harvested stage) of king oyster mushroom (Pleurotus eryngii) produced by bottle type cultivation and oyster mushroom (Pleurotus osteratus) produced by vinyl bag type cultivation. For both cultivation types, compared with the initial culture, the weight reduction rate in spent mushroom substrates (SMS) after fruit body harvest was 29% for total wet mass, 21～25% for dry and organic matters and 19 ～22% for neutral detergent fiber. Two thirds to 3/4 of organic matter degraded and utilized by mycelia and fruit bodies was originated from fiber, of which the primary source (50～70%) was hemicellulose. The effect of mycelial growth stage on chemical compositional change in culture was little (P>0.05) for bottle type cultivation of king oyster mushroom but considerable (P<0.05) for vinyl type cultivation of oyster mushroom. Culture nutrients uptake by fruit bodies was very active for the bottle type cultivation. Compared with SMS, harvested fruit bodies (mushrooms) contained higher (P<0.05) crude protein, non-fibrous carbohydrate, and crude ash and lower (P<0.05) neutral detergent fiber. Regardless of stages, no culture samples were contaminated with toxic heavy metals and pesticide residues. In conclusion, the increase of fiber (neutral and acid detergent fibers) and indigestible protein contents and the decrease of true protein content in SMS indicated that the feed-nutritional value of SMS was significantly reduced compared with that of the initial culture and they were safe from toxic heavy metals and pesticide residues.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.109-120
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• 2007

CLA was chemically synthesized by alkaline isomerization method using corn oil. CLA-TG was synthesized by chemical reaction using sodium methoxide. For the control, 10% of back fat among the total component was only added without the annex of CLA-TG. For the first treatment, 5% of CLA-TG among the lard component added into the press ham was replaced. For the 2nd, 3rd and 4rd treatments, 10%, 15% and 20% of CLA-TG was respectively replaced. Manufacture press ham using CLA-TG were vacuum packaged and then stored during 1, 7, 14, 21 and 28 days at 4℃. Samples were analyzed for shear force value, sensory evaluation, TBARS, fatty acid composition and CLA content. Shear force value of control was significantly higher than that of CLA-TG treatment groups(P<0.05). All treatments were increased by the passage of storage time. No remarkable differences were found in sensory properties among control and CLA-TG treatment groups. CLA-TG treatment groups showed significantly(P<0.05) lower TBARS value than the control. TBARS value was increased significantly during storage in all treatment. In the change of fatty acid composition, the contents of C14:0～C20:4 were decreased significantly by CLA-TG additive. Whereas the increase level of CLA-TG additive resulted in the significantly higher unsaturated fatty acid and CLA content. Summing up the a forementioned results, press ham manufacturing with CLA-TG additive was not affected in sensory evaluation. Also, it may be assumed that the high quality press ham can be manufactured with the extent of storage period and CLA accumulation.

• 한국유가공학회:학술대회논문집
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• 2002.04a
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• pp.59-60
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• 2002

Edible films such as wax coatings, sugar and chocolate covers, and sausage casings, have been used in food applications for years$^{(1)}$ However, interest in edible films and biodegradable polymers has been renewed due to concerns about the environment, a need to reduce the quantity of disposable packaging, and demand by the consumer for higher quality food products. Edible films can function as secondary packaging materials to enhance food quality and reduce the amount of traditional packaging needed. For example, edible films can serve to enhance food quality by acting as moisture and gas barriers, thus, providing protection to a food product after the primary packaging is opened. Edible films are not meant to replace synthetic packaging materials; instead, they provide the potential as food packagings where traditional synthetic or biodegradable plastics cannot function. For instance, edible films can be used as convenient soluble pouches containing single-servings for products such as instant noodles and soup/seasoning combination. In the food industry, they can be used as ingredient delivery systems for delivering pre-measured ingredients during processing. Edible films also can provide the food processors with a variety of new opportunities for product development and processing. Depends on materials of edible films, they also can be sources of nutritional supplements. Especially, whey proteins have excellent amino acid balance while some edible films resources lack adequate amount of certain amino acids, for example, soy protein is low in methionine and wheat flour is low in lysine$^{(2)}$. Whey proteins have a surplus of the essential amino acid lysine, threonine, methionine and isoleucine. Thus, the idea of using whey protein-based films to individually pack cereal products, which often deficient in these amino acids, become very attractive$^{(3)}$. Whey is a by-product of cheese manufacturing and much of annual production is not utilized$^{(4)}$. Development of edible films from whey protein is one of the ways to recover whey from dairy industry waste. Whey proteins as raw materials of film production can be obtained at inexpensive cost. I hypothesize that it is possible to make whey protein-based edible films with improved moisture barrier properties without significantly altering other properties by producing whey protein/lipid emulsion films and these films will be suitable far food applications. The fellowing are the specific otjectives of this research: 1. Develop whey protein/lipid emulsion edible films and determine their microstructures, barrier (moisture and oxygen) and mechanical (tensile strength and elongation) properties. 2. Study the nature of interactions involved in the formation and stability of the films. 3. Investigate thermal properties, heat sealability, and sealing properties of the films. 4. Demonstrate suitability of their application in foods as packaging materials. Methodologies were developed to produce edible films from whey protein isolate (WPI) and concentrate (WPC), and film-forming procedure was optimized. Lipids, butter fat (BF) and candelilla wax (CW), were added into film-forming solutions to produce whey protein/lipid emulsion edible films. Significant reduction in water vapor and oxygen permeabilities of the films could be achieved upon addition of BF and CW. Mechanical properties were also influenced by the lipid type. Microstructures of the films accounted for the differences in their barrier and mechanical properties. Studies with bond-dissociating agents indicated that disulfide and hydrogen bonds, cooperatively, were the primary forces involved in the formation and stability of whey protein/lipid emulsion films. Contribution of hydrophobic interactions was secondary. Thermal properties of the films were studied using differential scanning calorimetry, and the results were used to optimize heat-sealing conditions for the films. Electron spectroscopy for chemical analysis (ESCA) was used to study the nature of the interfacial interaction of sealed films. All films were heat sealable and showed good seal strengths while the plasticizer type influenced optimum heat-sealing temperatures of the films, 130$^{\circ}$C for sorbitol-plasticized WPI films and 110$^{\circ}$C for glycerol-plasticized WPI films. ESCA spectra showed that the main interactions responsible for the heat-sealed joint of whey protein-based edible films were hydrogen bonds and covalent bonds involving C-0-H and N-C components. Finally, solubility in water, moisture contents, moisture sorption isotherms and sensory attributes (using a trained sensory panel) of the films were determined. Solubility was influenced primarily by the plasticizer in the films, and the higher the plasticizer content, the greater was the solubility of the films in water. Moisture contents of the films showed a strong relationship with moisture sorption isotherm properties of the films. Lower moisture content of the films resulted in lower equilibrium moisture contents at all aw levels. Sensory evaluation of the films revealed that no distinctive odor existed in WPI films. All films tested showed slight sweetness and adhesiveness. Films with lipids were scored as being opaque while films without lipids were scored to be clear. Whey protein/lipid emulsion edible films may be suitable for packaging of powder mix and should be suitable for packaging of non-hygroscopic foods$^{(5,6,7,8,)}$.

• Korean Journal of Environmental Agriculture
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• v.13 no.2
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• pp.223-230
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• 1994

A practical study on a drying and fermentation system equipped with a stirring machine operated mechanically, of pig manure was conducted to prove the efficiency of and practicability to an ordinary pig farm. The type of the drying bed was a round-shaped (r=3m) concrete structure and the stirring machine was adopted to stir and transfer dried pig manure to the fermentation tank. The dried pig manure was put into a fermentation tank ($V=18m^3$), which was aerated from pipe lines installed at the bottom. While water content of pig manure passing through a drying bed was remarkably reduced than before drying, the drying efficiency of this system decreased in winter. However, the temperature of pig manure piled up in the fermentation room in winter reached over $60^{\circ}C$ and excess water of pig manure was removed during the fermentation process. The reduction rate of water content of pig manure, to which dried pig manure was added as bulking material on the drying bed, was 52.1%, but when dried without bulking material it was only 19.7%. Although the content of $P_2O_5$ of dried pig manure was slightly higher than that of fresh pig manure, progressive changes in chemical composition between fresh and dried pig manure made no great difference. Among the contents of minerals of fresh and dried pig manure, CaO was the highest and the rest were in the decreasing order of $K_2O$, MgO, and $Na_2O$. Population density of E. coli and Streptococci of dried pig manure was reduced by 142 and 236 times that of fresh pig manure, respectively. The installation cost of this drying and fermentation system was 4,185,630 won (approximately 5,232 US $) and operating cost per year was 190,000 won (237.5US $) on the basis of self-labor condition.

• The Korean Journal of Pesticide Science
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• v.18 no.1
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• pp.33-40
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• 2014

To control Bemisia tabaci on tomato, we applied five different combinations of chemical treatments as below: 1) treatment of combinations of cyantraniliprole on the root area and leaf with the existing registered chemicals three times; 2) treatment of combinations of cyantraniliprole on the root area and dinotefuran + emamectin benzoate on the leaf with the existing registered chemicals three times; 3) treatment of combinations of dinotefuran on the root area and cyantraniliprole on the leaf with the existing registered chemicals three times; 4) treatment of combinations of dinotefuran on the root area and dinotefuran + emamectin benzoate on the leaf with the existing registered chemicals three times; 5) untreated control plot (Table 1). Twenty days after treatment ($17^{th}$ Aug.), the number of population of B. tabaci was zero on the 1, 2, 3, 4 treatments of combinations, and only 2 individuals were found on the 5 treatment of combination per each 20 plant. On $17^{th}$ Sep., in the last observation, the average number of population of B. tabaci was 10.3, 10.3, 10.6 on the 1, 2, 3 treatments of combinations on the 20 plants per each combination, however, the average number of 23.3 and 37.6 were examined on the 4 and 5 treatments of combinations, respectively. TYLCV was not occurring on the 1 and 2 treatments of combinations, and presented only 3% and 17% on the 3 and 4 treatments of combinations, respectively, which indicates that the treatments (1-4) should be effective on TYLCV control as considering that 33% of TYLCV occurred on the untreated control plot. However, after the third flowering period, there is no difference among the five combinations. The amount of products was 9,148g and 9,698g on the 1 and 2 treatments of combinations, respectively, which was the most among the 5 combinations. The number of fallen fruits and the average weight of fruits showed the similar tendency.

• Journal of Food Hygiene and Safety
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• v.32 no.1
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• pp.1-7
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• 2017

This study was conducted to investigate the microbial loads in mulberry fruits depending on cultivation environment and fruit ripeness. The population levels of total aerobic bacteria in mulberry fruits collected from open field orchards were higher than those from three plots protected within plastic green houses. In regards to fruit ripeness, the levels of total aerobic bacteria in ripe black fruits were higher than those in unripe green and red mulberry. From the farms into where livestock animals were allowed to enter, Escherichia coli was detected in soil at a level of 4.26~4.94 log CFU/g and in mulberry fruits at 5.03~6.07 log CFU/g, while no coliform and E. coli were detected from where the intrusion of livestock was prevented. We also examined the density change of inoculated E. coli in mulberry fruits as they were becoming mature. While E. coli did not increase in green fruits, two and four log CFU/g increases at $20^{\circ}C$ and $37^{\circ}C$, respectively, were observed with red and fully mature black mulberries during 48 hours incubation. To ensure the food safety of mulberry, it is suggested that the introduction of E. coli into a farm through livestock should be prevented and more hygienic caution should be taken especially when the fruits are ripe.

• The Journal of the Korean Society for Microbiology
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• v.21 no.3
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• pp.311-329
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• 1986

The experimental exposure of animals to sources of ultraviolet radiation (UVR) which emit their energy primarily in the UVB region (280-320nm) is known to result in a number of well-described changes in the recipient's immune competence. Two such changes include a depressed capacity to effectively respond immunologically to transplants of syngeneic UVR tumors and a markedly reduced responsiveness to known inducers of delayedtype (DTH) and contact hypersensitivity (CH) reactions. The results of experiments that were designed to elucidate the mechanisms responsible for UVR-induced immunomodulation have implicated: 1) an altered pattern of lymphocyte recirculation, 2) suppressor T cells(Ts), 3) deviations in systemic antigen presenting cell (APC) potential. 4) changes in the production of interleukin-1-like molecules, and 5) the functional inactivation of epidermal Langerhans cells in this process. The exposure of skin to UVR, therefore, causes a number of both local and systemic alterations to the normal host immune system. In spite of this seeming complexity and diversity of responses, our recent studies have established that each of the UVR-mediated changes is probably of equal importance to creating the UVR-induced immunocompromised state. Normal animals were exposed to low dose UVR radiation on their dorsal surfaces under conditions where a $3.0\;cm^2$ area of skin was physically protected from the light energy. Contact sensitization of these animals with DNFB, to either the irradiated or protected back skin, resulted in markedly reduced CH responses. This was observed in spite of a normal responsiveness following the skin sensitization to ventral surfaces of the UVR-exposed animals. Systemic treatment of the low dose UVR recipients with the drug indomethacin (1-3 micrograms/day) during the UVR exposures resulted in a complete reversal of the depressions observed following DNFB sensitization to "protected" dorsal skin while the altered responsiveness found in the group exposed to the skin reactive chemical through directly UVR-exposed sites was maintained. These studies implicate the importance of EC as effective APC in the skin and also suggest that some of the systemic influences caused by UVR exposure involve the production of prostaglandins. This concept was further supported by finding that indomethacin treatment was also capable of totally reversing the systemic depressions in CH responsiveness caused by high dose UVR exposure (30K joules/$m^2$) of mice. Attempts to analyze the cellular mechanisms responsible established that the spleens of all animals which demonstrated altered CH responses, regardless of whether sensitization was through a normal or an irradiated skin site, contained suppressor cells. Interestingly, we also found normal levels of T effector cells in the peripheral lymph nodes of the UVR-exposed mice that were contact sensitized through normal skin. No effector cells were found when skin sensitization took place through irradiated skin sites. In spite of such an apparent paradox, insight into the probable mechanisms responsible for these observations was provided by establishing that UVR exposure of skin results in a striking and dose-dependent blockade of the efferent lymphatic vessels in all peripheral lymph nodes. Therefore, the afferent phases of immune responses can apparently take place normally in UVR exposed animals when antigen is applied to normal skin. The final effector responses, however, appear to be inhibited in the UVR-exposed animals by an apparent block of effector cell mobility. This contrasts with findings in the normal animals. Following contact sensitization, normal animals were also found to simultaneously contain both antigen specific suppressor T cells and lymph node effector cells. However, these normal animals were fully capable of mobilizing their effector cells into the systemic circulation, thereby allowing a localization of these cells to peripheral sites of antigen challenge. Our results suggest that UVR is probably not a significant inducer of suppressor T-cell activity to topically applied antigens. Rather, UVR exposure appears to modify the normal relationship which exists between effector and regulatory immune responses in vivo. It does so by either causing a direct reduction in the skin's APC function, a situation which results in an absence of effector cell generation to antigens applied to UVR-exposed skin sites, inhibiting the capacity of effector cells to gain access to skin sites of antigen challenge or by sequestering the lymphocytes with effector cell potential into the draining peripheral lymph nodes. Each of these situations result in a similar effect on the UVR-exposed host, that being a reduced capacity to elicit a CH response. We hypothesize that altered DTH responses, altered alloresponses, and altered graft-versus-host responses, all of which have been observed in UVR exposed animals, may result from similar mechanisms.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.1-6
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• 2003

This study was determined to the growth characteristic, yield and chemical content of Azuki bean(Vigna angularis W.F. $W_{IGHT}$) based on five phosphate rates (0, 40, 50, 120, 160, 200kg/ha) from May, 2002 to August, 2002 in Jeju province. Plant height was 80.7cm at 0kg/ha of phosphate level md, as phosphate rates were increased to 160kg/ha and 200kg/ha, lengthened 88.8cm and 88.9cm, respectively, but between the two phosphat levels were no significance(P＞0.05 or 0.0l). Numbs. of branches and leaves per plant, stem diameter, and weight of leaves and stems per plant were the same response with plant height. Fresh forage, dry matter, crude protein, and TDN(total digestible nutrient) yield at the control were 23.8MT/ha, 3MT/ha, 0.5MT/ha and 1.8MT/ha, respectively, at the 200kg/ha plot were 47.3MT/ha, 7.2MT/ha, 1.3MT/ha, and 4.7MT/ha, respectively, as phosphate rate was increased. As phosphate rate increased from 0kg/ha to 200kg/ha, the content of crude protein, crude fat NFE(nitrogen free extract) and TDN increased 15.2%~18.6%, 3.4%~4.5%, 41.4%~45.5% and 58.3%~65.5%, respectively, whereas the content of crude fiber and crude ash were decreased 31.5%~24.8% and 8.1%~6.6%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.13-22
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• 2003

This pot experiment was conducted to find out the forage productivity and mineral contents in an orchardgrass sward affected by the compost, lime, and phosphorus applications on the newly reclaimed and arable pasture soils. This 3rd part was concerned with the effect of phosphorus application($P_{0}$ : control, $P_1$=1.25ｇ, $P_2$=2.50g, $P_3$=3.75g $P_2$$O_{5}$ pot). The results obtained are summarized as fellows; There were considerable differences between the newly reclaimed and arable pasture soils in the soil chemical characteristics as follows in general; poor~common in the newly reclaimed soil and common~good in the arable pasture soil in terms of soil fertility standard. Comparing with the arable pasture soil, the contents of available$ P_2$$O_{5}$ / in the newly reclaimed soil at end of experiment were considerably increased by the $P_2$$O_{5}$ applications, These effects in the newly reclaimed soil were positively influenced by the additional application of compost, but were adversely influenced by the additional application of lime. The seedling vigour and yield of orchardgrass were far better in the arable pasture soil than in the newly reclaimed soil. At the $P_{0}$ treatment, those were very poor in 也e newly reclaimed soil, but were good in the arable pasture soil. Comparing with $P_1$ treatment, the above effects of heavy $P_2$$O_{5}$ / applications($P_2$and $P_3$) were not recognized. On the newly reclaimed soil, the above effects were markedly enhanced by the applications of $P_2$$O_{5}$ with compost. The Ca, Mg, and P contents of orchardgrass were relatively higher on the arable pasture soil than on the newly reclaimed soil, whereas ere were no differences in the K contents.