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Detection of immunity in sheep following anti-rabies vaccination

  • Hasanthi Rathnadiwakara;Mangala Gunatilake;Florence Cliquet;Marine Wasniewski;Mayuri Thammitiyagodage;Ramani Karunakaran;Jean-Christophe Thibault;Mohamed Ijas
    • Clinical and Experimental Vaccine Research
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    • v.12 no.2
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    • pp.97-106
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    • 2023
  • Purpose: Rabies is a fatal but preventable disease with proper pre-exposure anti-rabies vaccination (ARV). Dogs, as household pets and strays, are the reservoir and vector of the disease, and dog bites have been associated with human rabies cases in Sri Lanka over the past few years. However, other susceptible species having frequent contact with humans may be a source of infection. One such species is sheep and immunity following ARV has never been tested in sheep reared in Sri Lanka. Materials and Methods: We have tested serum samples from sheep reared in the Animal Centre, Medical Research Institute of Sri Lanka for the presence of anti-rabies antibodies following ARV. Sheep serum samples were tested with Bio-Pro Rabies enzyme-linked immunosorbent assay (ELISA) antibody kits used for the first time in Sri Lanka and our results were verified by a seroneutralization method on cells (fluorescent antibody virus neutralization, FAVN test) currently recommended by World Organization for Animal Health and World Health Organization. Results: Sheep received annual ARV and maintained high neutralizing antibody titers in their serum. No maternal antibodies were detected in lamb around 6 months of age. Agreement between the ELISA and FAVN test, i.e., coefficient concordance was 83.87%. Conclusion: Annual vaccination in sheep has an effect on maintaining adequate protection against rabies by measurements of anti-rabies antibody response. Lambs need to be vaccinated earlier than 6 months of age to achieve protective levels of neutralizing antibodies in their serum. Introducing this ELISA in Sri Lanka will be a good opportunity to determine the level of anti-rabies antibodies in animal serum samples.

Sustainable diets: a scoping review and descriptive study of concept, measurement, and suggested methods for the development of Korean version (지속가능한 식이의 개념과 측정방법 및 한국형 식이 지수 개발을 위한 방안 모색: 주제범위 문헌고찰과 기술 연구)

  • Sukyoung Jung
    • Korean Journal of Community Nutrition
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    • v.29 no.1
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    • pp.34-50
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    • 2024
  • Objectives: Transformation through a sustainable food system to provide healthy diets is essential for enhancing both human and planetary health. This study aimed to explain about sustainable diets and illustrate appropriate measurement of adherence to sustainable diets using a pre-existing index. Methods: For literature review, we used PubMed and Google Scholar databases by combining the search terms "development," "validation," "sustainable diet," "sustainable diet index," "planetary healthy diet," "EAT-Lancet diet," and "EAT-Lancet reference diet." For data presentation, we used data from National Health and Nutrition Examination Survey, 2017-2018, among adults aged 20 years and older (n = 3,920). Sustainable Diet Index-US (SDI-US), comprising four sub-indices corresponding to four dimensions of sustainable diets (nutritional quality, environmental impacts, affordability, and sociocultural practices), was calculated using data from 24-hour dietary recall interview, food expenditures, and food choices. A higher SDI-US score indicated greater adherence to sustainable diets (range: 4-20). This study also presented SDI-US scores according to the sociodemographic status. All analyses accounted for a complex survey design. Results: Of 148 papers, 16 were reviewed. Adherence to sustainable diets fell into 3 categories: EAT-Lancet reference diet-based (n = 8), Food and Agriculture Organization (FAO) definition-based (n = 4), and no specific guidelines but including the sustainability concept (n = 4). Importantly, FAO definition emphasizes on equal importance of four dimensions of diet (nutrition and health, economic, social and cultural, and environmental). The mean SDI-US score was 13 out of 20 points, and was higher in older, female, and highly educated adults than in their counterparts. Conclusions: This study highlighted that sustainable diets should be assessed using a multidimensional approach because of their complex nature. Currently, SDI can be a good option for operationalizing multidimensional sustainable diets. It is necessary to develop a Korean version of SDI through additional data collection, including environmental impact of food, food price, food budget, and use of ready-made products.

Numerical simulation of infill CACB wall cracking subjected to wind loads

  • Ruige Li;Yu Gao;Hongjian Lin;Mingfeng Huang;Chenghui Wang;Zhongzhi Hu;Lingyi Jin
    • Structural Engineering and Mechanics
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    • v.89 no.5
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    • pp.479-489
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    • 2024
  • The cracking mechanism in ceramsite aerated concrete block (CACB) infill walls were studied in low seismic fortification intensity coastal areas with frequent occurrence of typhoons. The inter-story drifts of an eight-story residential building under wind loads and a seismic fortification intensity of six degrees were analyzed by using the PKPM software. The maximum inter-story drift ratio of the structure in wind load was found to be comparable to that under the seismic fortification intensity of six degrees. However, when accounting for the large gust wind speed of typhoon, the maximum inter-story drift ratio was much larger than that obtained under reference wind load. In addition, the finite element models of RC frames were employed by displacement loading to simulate two scenarios with and without window hole in the CACB infill walls, respectively. The simulation results show no signs of cracking in both the infill walls with window hole and those without window for the inter-story drift caused by seismic loads and the reference wind load. However, both types of infill walls experienced structural creaking when assessing the gust wind pressure recorded from previous typhoon monitoring. It is concluded that an underestimate of wind loads may contribute substantially to the cracking of frame CACB infill walls in low seismic fortification intensity coastal areas. Consequently, it is imperative to adopt wind pressure values derived from gust wind speeds in the design of CACB infill walls within frame structures. Finally, the future research directions of avoiding cracks in CACB filled walls were proposed. They were the material performance improving and building structure optimizing.

Palatal vault configuration and its influence on intraoral scan time and accuracy in completely edentulous arches: a prospective clinical study

  • Dina Mohamed Ahmed Elawady;Wafaa Ibrahim Ibrahim;Radwa Gamal Ghanem;Reham Bassuni Osman
    • The Journal of Advanced Prosthodontics
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    • v.16 no.4
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    • pp.201-211
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    • 2024
  • PURPOSE. The aim of this prospective clinical study was to compare the influence of palatal vault forms on accuracy and speed of intraoral (IO) scans in completely edentulous cases. MATERIALS AND METHODS. Based on the palatal vault form, participants were divided into three equal groups (n = 10 each); Class I: moderate; Class II: deep; Class III: flat palatal vault. A reference model was created for each patient using polyvinylsiloxane impression material. The poured models were digitized using an extraoral scanner. The resultant data were imported as a solid CAD file into 3D analysis software (GOM Inspect 2018; Gom GmbH, Braunschweig, Germany) and aligned using the software's coordinate system to determine its X, Y, and Z axes. Five digital impressions (DIs) of maxilla were captured for each patient using an intraoral scanner (TRIOS; 3Shape A/S, Copenhagen, Denmark) and the resultant Standard Tessellation Language (STL) scan files served as test models. Trueness was evaluated by calculating arithmetic mean deviation (AMD) of the vault area between reference and test files while precision was evaluated by calculating AMD between captured scans to measure repeatability of scan acquisition. The scan time taken for each participant was also recorded. RESULTS. There was no significant difference in trueness and precision among the groups (P = .806 and .950, respectively). Average scan time for Class I and III palatal vaults was 1 min 13 seconds and 1 min 37 seconds, respectively, while class II deep palatal vaults showed the highest scan time of 5 mins. CONCLUSION. Palatal vault form in edentulous cases has an influence on scan time. However, it does not have a substantial impact on the accuracy of the acquired scans.

Growth Performance, Humoral Immune Response and Carcass Characteristics of Broiler Chickens Fed Alkali Processed Karanj Cake Incorporated Diet Supplemented with Methionine

  • Panda, K.;Sastry, V.R.B.;Mandal, A.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.677-681
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    • 2005
  • A study was conducted to see the effect of dietary incorporation of alkali (1.5% NaOH, w/w) processed solvent extracted karanj cake (SKC) supplemented with methionine on growth performance, humoral immune response and carcass characteristics of broiler chickens from 0 to 8 weeks of age. One hundred and twenty, day- old broiler chicks were wing banded, vaccinated against Marek' disease and distributed in a completely randomized design (CRD) into 3 groups of 40 chicks each, which was further replicated to 4 and fed on diet containing soybean meal and those of test groups were fed diets containing alkali (1.5% NaOH) treated SKC partially replacing soybean meal nitrogen of reference diet (12.5%) without or with supplementation of methionine (0.2%). Individual body weight of chicks and replicate-wise feed intakes were recorded at weekly intervals throughout the experimental period. Feed consumption from 1 to 14, 28, 42 and 56 d of age was recorded for each replicate and feed conversion efficiency (weight gain/feed intake) for the respective period was calculated. Mortality was monitored on daily basis. On 28$^{th}$ day of experimental feeding, two birds of each replicate in each dietary group (8 birds/diet) were inoculated with 0.1 ml of a 1.0% suspension of sheep red blood cells (SRBC) and the antibody titre (log 2) was measured after 5 days by the microtitre haemmagglutination procedure. After 42 days of experimental feeding, a retention study of 4 days (43-47 d) duration was conducted on all birds to determine the retention of various nutrients such as DM, N, Ca, P and GE. On 43$^{rd}$ day of experimental feeding, one representative bird from each replicate of a dietary treatment (4/dietary group) was sacrificed, after fasting for two hours with free access to water, through cervical dislocation to observe the weight of dressed carcass, primal cuts (breast, thigh, drumstick, back, neck and wing), giblet (liver, heart and gizzard), abdominal fat and digestive organs. The body weight gain of chicks fed reference diet and those fed diet incorporated with NaOH treated SKC (12.5% replacement) with or without methionine supplementation was comparable during 0 to 4 weeks of age. However, dietary incorporation of alkali processed SKC replacing 12.5% nitrogen moiety of soybean meal resulted in growth retardation, subsequently as evidenced by significantly (p<0.05) lowered body weight gain during 0 to 6 weeks of age in birds fed diet incorporated with alkali processed SKC at 6.43% without methionine as compared to those supplemented with methionine or reference diet. Dietary incorporation of alkali (1.5% NaOH) processed SKC replacing 12.5% of soybean meal nitrogen in the diet of broiler chickens had no adverse effect on feed conversion ratio during all the weeks of experimental feeding. The humoral immune response (HIR) as measured by the antibody titre in response to SRBC inoculation was comparable among all the dietary groups. No significant difference in the intake and retention of DM, N, Ca, P or GE was noted among the chicks fed reference and alkali processed SKC incorporated diets with or without methionine supplementation. None of the carcass traits varied significantly due to dietary variations, except the percent weight of liver and giblet. The percent liver weight was significantly (p<0.05) higher in the birds fed diet incorporated with alkali processed SKC as compared to that in other two groups. Thus solvent extracted karanj cake could be incorporated after alkali (1.5% NaOH, w/w) processing at an enhanced level of 6.43%, replacing 12.5% of soybean meal nitrogen, in the broiler diets up to 4 weeks of age, beyond which the observed growth depression on this diet could be alleviated by 0.2% methionine supplementation.

Changes of Shoulder Muscles Activity during Maintaining and Lifting Shoulder Depending on Stretch Rate of Kinesio Tape

  • Kim, Ju-Seung;Park, Min-Chull
    • The Journal of Korean Physical Therapy
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    • v.27 no.5
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    • pp.299-303
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    • 2015
  • Purpose: The objective of this study was to examine the changes in muscle activity on the anterior deltoid muscle depending on the stretch rate of kinesio tape when applying kinesio tape in healthy adult subjects. Methods: This study was a single-blind randomized controlled trial, including 22 healthy participants (male 15, female 7) with no pathology or past history of shoulder who participated voluntarily. Participants applied a different stretch rate of the kinesio tape, and the functional activity was tested. The stretch rate of kinesio tape was 0%, 10%, and 20%. Subjects lifted a weight (5% of their body weight) to their shoulder height. Subjects lifted a weight up to an angle of 90 degrees in the sagittal plane, and muscle activities (biceps brachii, anterior deltoid, middle deltoid, upper trapezius) were assessed using EMG (electromyography). Analysis of muscle activity was divided into two parts (lift weight and keep holding). The EMG activity of each muscle was normalized to the value of reference voluntary contraction (%RVC) and explored using repeated ANOVA. Results: There were no significant differences in muscle activity depending on the ratio of tape stretch when lifting a weight (p>0.05). There were no significant differences in muscle activity depending on the ratio of tape stretch when holding a weight (p>0.05). Conclusion: According to the results of this study, in the case of applying kinesio taping therapy for healthy people, it was found that the stretch rate of the tape does not have an effect on muscle activity.

A Study on The Reproducibility of Centric Relation Taken with Three Methods Using Split Cast Technique (스플릿 캐스트법을 이용한 중심위의 채득방법에 따른 재현성에 관한 연구)

  • Kay, Kee-Sung;Kim, Young-Suk;An, Jong-Kwan
    • Journal of Dental Rehabilitation and Applied Science
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    • v.17 no.4
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    • pp.275-282
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    • 2001
  • Centric relation is defined the horizontal position between maxilla and mandible when condylar head of mandible is positioned adequately in mandibular fossa. The most recent concept of centric relation position is defined as the mandibular position in which the condyles are in their most superoanterior position in the articular fossa, resting against the posterior slope of the articular eminences, with the articular disk properly interposed. To be suitable as a reference point during occlusal management, a mandibular position of centric relation has to be functionally acceptable to the patient and clinically reproducible and achievable during everyday practice. There are numerous methods for determination of centric relation, and in this study we used three of them, Gothic arch tracing(Group I), leaf gauge(Group II), and anterior jig(Group III). The subjects were 10, 8 men and 2 women, age-ranged from 23 to 26 years old, had no prosthetics in thier mouth, and had no sign and symptom of temporomandibular disorders. We gained three occlusal records using each method, and then the degree of the reproducibility was examined with split cast technique. In this study the reproducibility of centric relation using split cast technique was greater in the order of Group I(mean 1.6), Group II(mean 1.4), and Group III(mean 1.3), but there was no significant differences among them statistically(p>0.05).

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Comparison of Therapeutic Efficacy and Tolerance Between Nemonapride and Haloperidol in-Schizophrenic Patients (정신분열증환자에서 Nemonapride와 Haloperidol의 치료결과 및 내약성에 대한 비교분석)

  • Sung, Sang Kyung;Hong, Kwang Wha
    • Korean Journal of Biological Psychiatry
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    • v.2 no.1
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    • pp.123-130
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    • 1995
  • A single-blind comparative study was performed using haloperidol as a reference drug in order to evaluate the efficacy and safety of nemonapride, a new benzamide derivative, in sixty-nine Korean schizophrenic patients. the total period of the study was 8 weeks, maximum dosage of nemonapride was 36mg and that of haloperidol was 24mg. Psychopathology and extrapyramidal symptoms were assessed every week or four weeks until the end of the 8th week using the PANSS, BPRS, and 4 point general side effect check list, The drug safety was assessed every week until the end of the 8th week using vital sign, body weight, EEG, EKG, and blood chemistry. In total. one patient discontinued nemonapride treatment and seven patients discontinued haloperidol treatment before the end of the study. Therefore sixty-one patients(88 %) completed the study. PNASS and BPRS scores of the two groups on the end study point demonstrated a significant improvement compared with baseline score. The number of patients who had a clinical improvement of at least 20% in baseline score was similiar in both treatment groups. The difference of Simpson's rating scale socres were significant in both groups, and mean scores were more high in the haloperidol group than in nemonapride group. No significant EKG, EEG changes were induced, no relevant change in body weight or clinical laboratory parameters were observed in the sixty-one patients during 8 weeks and no Significant difference in the both groups. From these results, nemonapride is considered to be a clinically useful drug having a wide range of antipsychotic effect in schzophrenic patients.

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Isolation and Identification of Exo-xylanase Producing Microorganism (Exo-xylanase 생산균의 분리 및 동정)

  • 하재석;이영남;임재윤
    • Microbiology and Biotechnology Letters
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    • v.20 no.1
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    • pp.14-19
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    • 1992
  • The xylanase producing microorganisms occurring on rotten woods were selectively isolated on the modified Czapek-Dox medium supplemented with 0.5% xylan as a sole carbon source. Among more than three-hundred isolates of xylanase producing microorganisms, only two bacterial isolates were turned out to be more potent xylanase producer than the reference strain of xylanase producer, Aureobaszdium pullulans NRRL Y-2311. The exo-xylanase producer, bacterial isolate No. 33 was identified as a strain of Pseudomonas sp. on the basis of morphological and biochemical characterizations as well as cellular fatty acid composition. Optima of pH and of temperature for enzyme reactions of xylanase were 5.5 and $50^{\circ}C$ respectively. The enzyme was stable in a range of pH 5.0~7.0 and below $45^{\circ}C$. Among the number of carbohydrate substrates, xylose was turned out to be a potent inducer of Pseudomonas sp. No.33 exo-xylanase. Among the raw materials tested, rice straw was the best material for xylanase production by Pseudomonas sp. strain No. 33.

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A Study on the Relationship Between the Clothing Behavior and the MBTI Personality Type (여대생의 의복행동과 MBTI 성격유형에 관한 연구)

  • Jon, Myong-Sug;Kim, Yong-Im
    • Korean Journal of Human Ecology
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    • v.10 no.1
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    • pp.57-71
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    • 2001
  • The purpose of this study is to find the relationship between the clothing behavior and the MBTI personality type. I selected the college women of W college 96 grade as an object of this study for MBTI personality type test. It is analyzed to relation according to energy direction, perceiving function, behavior style, and function/temperament of psychology. This study presents the fashion, modesty, ostentation, extravagance, and aesthetic of clothing behavior in relation to MBTI personality type. The results of study is as follows : 1. There was the difference of significant level(P<0.05) between fashion and perceiving function on MBTI preference tendency of personality type. When person having a lot of fashion corrects a information, this person mostly uses the function of intuition among perceiving function. 2. There was the difference of significant level(P<0.05) between modesty and perceiving function on MBTI preference tendency of personality type. Modesty was deeply related to introversion of person having energy direction. 3. There was no difference of significant level between ostentation and energy direction, perceiving function, behavior style, decision function on MBTI reference tendency of personality type. 4. There was shown the difference of significant level(P<0.01) between extravagance and energy direction on MBTI preference tendency of personality type. Extravagance is deeply related to extraversion of person having external experience. 5. There was no difference between aesthetic and clothing behavior. 6. There was no difference between function / temperament of psychology and clothing behavior.

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