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Qualitative PCR Detection of vitamin E-enriched GM Perilla (비타민 E 강화 유전자변형 들깨에 대한 정성 PCR 분석법)

  • Kim, Jae-Hwan;Ahn, Ji-Hye;Song, Hee-Sung;Kim, Kyung-Hwan;Kim, Dong-Hern;Kim, Hae-Yeong
    • Applied Biological Chemistry
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    • v.49 no.3
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    • pp.192-195
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    • 2006
  • For the development of a qualitative PCR detection method for genetically modified perilla (Perilla frutescens), perilla species-specific gene, KAS-I (Beta-ketoacyl-ACP synthase I), was selected and validated as suitable for the use as an endogenous reference gene in perilla. Primer specificity was first tested by the means of qualitative PCR analysis. The primer pair Pfru3-F/R amplifying the perilla endogenous gene, KAS-I, gave rise to an amplicon 95 bp. No amplified product was observed when DNA samples from 15 different plants were used as templates. Qualitative PCR detection method was assayed with vitamin E-enriched GM Perilla developed in Korea. For the qualitative PCR detection method, the construct-specific detection primer pairs were constructed. The primer pair TMTO-F/R amplifying the junction region of TMT (${\gamma}$-tocopherol methyltransferase) gene and OCS (Octopine synthase) terminator introduced in GM perilla gave rise to an amplicon 148 bp.

The Analysis of the Level of Technological Maturity for the u-Learning of Public Education by Mobile Phone (휴대폰을 이용한 공교육 u-러닝의 기술 성숙도 분석)

  • Lee, Jae-Won;Na, Eun-Gu;Song, Gil-Ju
    • IE interfaces
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    • v.19 no.4
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    • pp.306-315
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    • 2006
  • In this paper we analyze whether we can use the mobile phone having been highly distributed into young generation as a device for the u-learning in Korean public education. For this purpose we deal with the technical maturity in three axes. Firstly, we examine the authoring nature of mobile internet-based contents such as both text and motion picture for the contents developers in the public education. As a research result the authoring of text has almost no difficulty, but that of the motion picture shows some problems. Secondly, we deal with whether u-learners can easily get and use u-contents on both mobile phone and PC respectively. After analysing this factor, we found that the downloading of motion picture contents into mobile phone is very limited. Therfore we talk about the usability and problem of various PC Sync tools and propose their standardization. Finally, the needs of the introduction of the ubiquitous SCORM which could enable to reuse u-contents among different Korean telco’s mobile phones are discussed. Here we describe some functionality of both ubiquitous SCORM and u-LMS. Our study looks like almost the first work examining the technological maturity for the introduction of u-learning with mobile phone in Korean public education and it could be used as a reference for the study of any other wireless telecommunication-based u-learning other than mobile telecommunication.

Evaluation of Discomfort Glare Indices in Office Lighting (사무실 조명에서의 Discomfort Glare 계산법 적용 및 평가)

  • Hur, Nam-Don;Kim, Hoon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.16 no.6
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    • pp.13-21
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    • 2002
  • In the interior lighting, usually we refer to TAL and general lighting. The last one is that we generally adopt it in the interior lighting conditions. Here, glare is most important factor of them, especially in working place using VDT. Reference or development of estimation on glare has been studied in CIE, IESNA etc. and these of glare regulations now are applied differently in the interior lighting design or estimation on glare. But we have no glare regulation in detail, of course except G classification, which has limit of itself, so in my case it is impossible to apply in the interior lighting. On this paper, with some of main glare regulations in the world and our conditions in the interior lighting which are selected carefully, in an actual state it has been estimated So, it intended to blow which one is most suitable or what factors are considered of them, also show glare index which are appropriate to our interior lighting.

A Study on the Mechanical Properties of SM490A by FCAW Welding Attitude (SM490A의 FCAW 용접 자세별 형상에 관한 기계적 특성 연구)

  • Lim, Kwang Mook;Lee, Sung Ill
    • Journal of the Korean Society of Safety
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    • v.34 no.6
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    • pp.7-12
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    • 2019
  • Flux Cored Arc Welding (FCAW), which has been widely used in many industries, was developed in the 1950s to supplement shortcomings of the Shielded Metal Arc Welding (SMAW). FCAW has an advantage in that it can weld regardless of postures and give good quality results in the filed with many different working conditions. In this study, SM490A (rolled steel for welding structural purpose) with different thicknesses (L:25T+R:30T) were welded using FCAW. Then the mechanical properties (tension test, bending test, hardness test, impact test and macro test) were analyzed and the following conclusions were drawn. In the tensile test, it exceeds the KS standard tensile strength range (400~510) in all welding positions, which means there is a problem in the tensile force transmission performance. In the bending test, it was found that most of the specimens did not exhibit surface rupture or other defects during bending test and they exhibit sufficient toughness even after plastic deformation. In the hardness test, all the results were lower than the standard value of 350 Hv of KS B 0893, which means they have good hardness. In the impact test, all results were larger than the KS reference value of 27J. In the macro test, they showed uniform structure state by the shape of the weld, and there was risk of lamination because no internal defects, bubbles, or impurities were found on the surface of the weld.

Recognition and Demand Analysis of Agro-healing Services by Supply Types (치유농업 형태별 수요자 인식 및 수요분석)

  • Bae, Seung-Jong;Kim, Dae-Sik;Kim, Soo-Jin;Kim, Seong-Pil;Lee, Wang-Lok;Ryu, Jin-Seok;Kim, Jeong-Eun;Park, Sin-Ae
    • Journal of Korean Society of Rural Planning
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    • v.25 no.4
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    • pp.1-11
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    • 2019
  • This study conducted a survey on the recognition and demand such as recognition level, policy necessity, service demand and policy demand by supply types in order to provide the basic data for successful settlement of agro-healing services. According to the survey on awareness, 45.2% of respondents were aware of the healing farming, and 31.3% of respondents had experience in participating in the agro-healing services. 63.6% of respondents replied that they were experiencing reasons for participating in agro-healing services. Respondents who had no knowledge of agro-healing services responded that 76.7% of respondents said they would not participate. More than two-thirds of respondents in all types indicated that they needed agro-healing services. As a result of evaluating the maximum willingness to pay, there was a willingness to pay for farm work healing about 15,800 won, horticulture healing about 14,800 won, forest healing about 13,400 won, and animal assisted healing about 17,000 won. Improving accessibility and strengthening awareness were high priorities for inconveniences and improvements. 70.1% of the respondents said that policies for agro-healing services are needed. Development of agro-healing programs and contents was the first priority for support policy. The result of this study is expected to provide reference data that can be suggested for agro-healing policy establishment.

Safety Assessment on the Incineration Disposal of Regulation Exempt Waste by RESRAD Code (RESRAD 코드를 활용한 규제해제 폐기물 소각처분에 대한 안정성 평가)

  • Kim, Hui-Gyeong;Han, Sang-Wook;Park, Su-Ri;Kim, Byung-Jick
    • Journal of radiological science and technology
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    • v.41 no.1
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    • pp.67-73
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    • 2018
  • In this paper, risk assessment was conducted to verify self - disposal requirements by landfill for exempted incineration ash by using Resrad Ver.6.5 computer code. The result of risk assessment by landfill for the incineration by-product is that individual dose is $6.91{\times}10^{-2}{\mu}Sv\;y-1$ and collective dose is $3.475{\times}10^{-7}man-Sv\;y-1$. It proved that the result meets reference dose of individual dose $10{\mu}Sv\;y-1$ and collective dose 1 man-Sv y-1 for general public. According to the current 'Nuclear Safety Commission Notice [No. 2014-3]', it states that the exempted wastes can be disposed of by incineration, landfill and recycling. However, most of recently documents and papers related to exempted wastes are disposed of by landfill and recyling and it could not confirm the case of exempt by incineration. If the national consensus is derived and treating the waste by using process of incineration is activated, it could be considered to treat low level of radiation wastewater and activated carbon excluded from exempted waste because of nuclide $^3H$ and $^{14}C$.

Selection of oncoplastic surgical technique in Asian breast cancer patients

  • Shin, Eui Sun;Kim, Hyo In;Song, Seung Yong;Lew, Dae Hyun;Lee, Dong Won
    • Archives of Plastic Surgery
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    • v.45 no.1
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    • pp.37-44
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    • 2018
  • Background Oncoplastic surgery is being increasingly performed in Korean women; however, unlike Westerners, Korean women usually have small to moderate-sized breasts. To achieve better outcomes in reconstructed breasts, several factors should be considered to determine the optimal surgical method. Methods A total of 108 patients who underwent oncoplastic surgery from January 2013 to December 2016 were retrospectively investigated. We used various methods, including glandular tissue reshaping, latissimus dorsi (LD) flap transposition, and reduction oncoplasty, to restore the breast volume and symmetry. Results The mean weight of the tumor specimens was 40.46 g, and the ratio of the tumor specimen weight to breast volume was 0.12 g/mL in the patients who underwent glandular tissue reshaping (n=59). The corresponding values were 101.47 g and 0.14 g/mL, respectively, in the patients who underwent reduction oncoplasty (n=17), and 82.54 g and 0.20 g/mL, respectively, in those treated with an LD flap (n=32). Glandular tissue reshaping was mostly performed in the upper outer quadrant, and LD flap transposition was mostly performed in the lower inner quadrant. No major complications were noted. Most patients were satisfied with the aesthetic results. Conclusions We report satisfactory outcomes of oncoplastic surgical procedures in Korean patients. The results regarding specimen weight and the tumor-to-breast ratio of Asian patients will be a helpful reference point for determining the most appropriate oncoplastic surgical technique.

Optical and Electrical Properties of InAs Sub-Monolayer Quantum Dot Solar Cell

  • Han, Im-Sik;Park, Dong-U;No, Sam-Gyu;Kim, Jong-Su;Kim, Jin-Su;Kim, Jun-O
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.196.2-196.2
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    • 2013
  • 본 연구에서는 분자선 에피택시 (MBE)법으로 성장된 InAs submonolayer quantum dot (SML-QD)을 태양전지에 응용하여 광학 및 전기적 특성을 평가하였다. 본 연구에서 사용된 양자점 태양전지(quantum dot solar cell, QDSC)의 구조는 n+-GaAs 기판 위에 n+-GaAs buffer와 n-GaAs base layer를 차례로 성장 한 후, 활성영역에 InAs/InGaAs SML-QD와 n-GaAs spacer layer를 8주기 형성하였다. 그 위에 p+-GaAs emitter, p+-AlGaAs window layer를 성장하고 ohmic contact을 위하여 p+-GaAs 를 성장하였다. SML-QD 구조의 두께는 0.3 ML 이며, 이때 SML-QD의 적층수를 4 stacks 으로 고정하였다. SML-QD 와의 비교를 위하여 2.0 ML크기의 InAs자발 형성 양자점 태양전지(SK-QDSC)과 GaAs 단일 접합 태양전지 (reference-SC)를 동일한 성장조건에서 제작하였다. PL 측정 결과, 300 K에서 SML-QD의 발광 피크는 SK-QD 보다 고에너지에서 나타나는데(1.349 eV), 이것은 SML-QD가 SK-QD보다 작은 크기를 가지기 때문으로 사료된다. SML-QD는 single peak를 보이는 반면, SK-QD는 dual peaks (1.112 / 1.056 eV)을 확인하였다. SML-QD의 반치폭(full width at half maximum, FWHM)이 SK-QD에 비하여 작은 것으로 보아 SML-QD가 SK-QD보다 양자점 크기 분포의 균일도가 높은 것으로 해석된다. Illumination I-V 측정 결과, SML-QDSC의 개방 전압(VOC) 과 단락전류밀도(JSC)는 SK-QDSC의 값과 비교해 보면, 각각 47 mV와 0.88 mA/cm2만큼 증가하였다. 이는 SK-QD보다 상대적으로 작은 크기를 가진 SML-QD로 인해 VOC가 증가되었으며, SML-QD가 SK-QD 보다 태양광을 흡수할 수 있는 영역이 비교적 적지만, QD내에 존재하는 energy level에서 탈출 할 수 있는 확률이 더 높음으로써 JSC가 증가한 것으로 분석 된다.

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Evaluation of the Agreement between Immunodot Assays and Intradermal Skin Testing or Favrot Diagnostic Criteria in Canine Atopic Dermatitis

  • Cho, Sun-Hyung;Kang, Ji-Houn;Yang, Mhan-Pyo;Kang, Byeong-Teck
    • Journal of Veterinary Clinics
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    • v.33 no.5
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    • pp.270-273
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    • 2016
  • This study was undertaken to identify differences between atopic and non-atopic dogs in three rapid screening immunodot assays as well as the ability of the assays to predict the results of intradermal skin testing (IDST) or Favrot diagnostic criteria (FDC). Twenty-nine dogs diagnosed with canine atopic dermatitis (CAD) were selected as the atopic group. Twenty-five dogs without CAD were included as the non-atopic group. Three types of immunodot assays were conducted on all serum samples from both groups: Allercept E-screen 2nd generation (ES2G), Canine Allergic Tendency Reference Test (ALERT), and Asan Easy Test Canine IgE (AETC). IDST, which included 39 allergens, and immunodot assays were performed concurrently in 13 dogs from the atopic group and compared. While there were no significant differences in positivity between the two groups in the evaluation of ALERT (P = 0.435) and AETC (P = 0.313), positivity in ES2G testing was significantly higher in the non-atopic group than the atopic group (P = 0.038). The ES2G, ALERT, and AETC results showed fair (${\kappa}=0.235$), slight (${\kappa}=0.133$), and slight (${\kappa}=0.014$) accordance with IDST, respectively. The outcomes of ES2G, ALERT, and AETC indicated poor (${\kappa}=-0.211$), slight (${\kappa}=0.106$), and slight (${\kappa}=0.087$) agreement with FDC. In conclusion, rapid screening immunodot assays were not useful for the diagnosis of CAD. These assays may provide a supplementary method for predicting the results of IDST in atopic dogs.

Conservation of Seated Iron Śākyamuni Buddha Statue from Goryeo Dynasty (고려(高麗) 철제석가여래좌상(鐵製釋迦如來坐像)의 보존)

  • Huh, Ilkwon;Yoo, Jayoung
    • Conservation Science in Museum
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    • v.11
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    • pp.9-16
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    • 2010
  • The National Chuncheon Museum has carried out a conservation process in order to prevent corrosion of the seated iron Śākyamuni Buddha statue from Goreyo Dysnasty for its exhibition. Before the conservation process, the surface of the artifact showed exfoliation and the artifact was damaged from rear to legs so exhibition was impossible. Therefore a process to get rid of pollution and to reinforce and protect the artifact was carried out. Before the reinforcing process, a basic test was carried out using micro crystalline wax type with reference to foreign and domestic experiment results. As a result, as wax(in xylene) 5wt% of Dongnam petrochemical Ltd. showed no efflorescence and little change in surface color and was convenient to use, it was chosen as a reinforcing agent and used to suppress corrosion. For the restoration of damaged parts, an internal support was made and used with an epoxy resin, allowing removable restoration, thus increasing effectiveness for exhibition.