• Title/Summary/Keyword: nitrogen ratio

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Influence of Varying Ruminally Degradable to Undegradable Protein Ratio on Nutrient Intake, Milk Yield, Nitrogen Balance, Conception Rate and Days Open in Early Lactating Nili-Ravi Buffaloes (Bubalus bubalis)

  • Nisa, Mahr-un;Javaid, A.;Shahzad, M. Aasif;Sarwar, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.9
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    • pp.1303-1311
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    • 2008
  • Twenty four early lactating Nili-Ravi buffaloes, eight in each group, were used in a Randomized Complete Block Design to evaluate the influence of varying ruminally degradable protein (RDP) to ruminally undegradable protein (RUP) ratio on feed intake, digestibility, N balance, milk yield and its composition, conception rate and days open. Three experimental diets were formulated to contain RDP:RUP of 50:50, 66:34 and 82:18 and were denoted as HRUP, MRUP and LRUP, respectively. Dry matter (DM) intake was higher (p<0.05) in buffaloes fed HRUP diet than in those fed MRUP and LRUP diets. Dry matter digestibility was higher (p<0.05) in buffaloes fed LRUP diet than in those fed HRUP and MRUP diets. Linear increase was observed in DM digestibility with increasing RDP:RUP while Neutral detergent fiber digestibility remained unaltered in buffaloes fed HRUP and MRUP diets, however, it was higher than in those fed LRUP diet. Crude protein digestibility remained unaltered across all treatments. Milk and 4 percent fat corrected milk (4% FCM) yield was higher (p<0.05) in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. Linear decrease in milk yield was observed with increased RDP:RUP. Milk protein and fat yields were higher (p<0.05) in animals fed HRUP diet than those fed MRUP and LRUP diets. Milk protein percent in animals fed HRUP diet was higher than in those fed LRUP diet, whereas it did not differ with those fed MRUP diet. Percent of fat, total solids, solid not fat and lactose remained unaltered across all diets. Nitrogen balance was higher in buffaloes fed HRUP diet than in those fed other diets. Increasing the RDP:RUP resulted in a linear decrease in N balance. The blood urea nitrogen and milk urea nitrogen were lower (p<0.05) in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. The blood pH remained unaltered across all treatments. Days open did not differ significantly. Conception rate was higher in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. The findings of the present study indicate that feeding high (50% of the total crude protein) ruminally undegradable protein diet not only increased nutrient intake and milk yield but also improved conception rate in early lactating buffaloes.

Effect of feeding tamarind kernel powder extract residue on digestibility, nitrogen availability and ruminal fermentation in wethers

  • Wang, Lin;Nakanishi, Takashi;Sato, Yoshiaki;Oishi, Kazato;Hirooka, Hiroyuki;Takahashi, Kei;Kumagai, Hajime
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.3
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    • pp.379-385
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    • 2017
  • Objective: This study was to examine in vivo digestibility, nitrogen balance and ruminal fermentation of tamarind (Tamarind indica) kernel powder extract residue (TKPER) compared to soybean products and by-products in wethers. Methods: Four wethers with initial body weight (BW) of $51.6{\pm}5.5kg$ were assigned in a $4{\times}4$ Latin square design to investigate nutritional characteristics of TKPER, dry heat soybean (SB), dry soybean curd residue (SBCR) and soybean meal (SBM) feeding with ryegrass straw (R) at a ratio of 1:1 at 2% of BW in dry matter (DM) on a daily basis. Results: The digestibility of DM, crude protein, and ether extract (EE) of TKPER-R diet were 57.0%, 87.0%, and 86.0%, respectively. Higher non-fiber carbohydrates digestibility was observed in TKPER-R diet (83.2%) than in SB-R diet (73.9%, p<0.05). Wethers fed the TKPER-R diet had lower retention of nitrogen (N) and ruminal ammonia nitrogen ($NH_3-N$) contents at 4 h after feeding than those fed the SBM-R diet (p<0.05), which had values similar to the SB-R or SBCR-R diet. The TKPER feeding had higher propionate (C3) and lower butyrate content, as well as lower acetate to propionate ratio (C2:C3) in rumen fluid than SBM feeding at 4 h after feeding (p<0.05). Conclusion: TKPER did not bring any side effect to the wethers although it was lack of fiber, and could be used as a high protein and energy ingredient in concentrate with appropriate roughage to meet the fiber requirement for ruminants.

The Physico-chemical Properties of Three Naked Barley Cultivars (Hordeum vulgare L.) Affected by Different Levels of Nitrogen Application (질소시용량의 차이가 과맥의 이화학적 특성에 미치는 영향)

  • ;Jae-Young Cho;Byong-Hee Hong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.3
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    • pp.242-253
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    • 1984
  • Four different levels of nitrogen were applied to 3 naked barley cultivars (Hordeum vulgare L.) Yonezawa, Baekdong and Yeongsanbori. Changes in physico-chemical properties were examined upon these treatments. Total protein content as well as glutelin increased as N level increased. Baekdong contained the highest protein content (13.7%) at N$_2$. Yonezawa and Baekdong contained more lysine than Yeongsanbori. Increased lysine was measured in all cultivars by increased nitrogen application. Among all amino acids proline was the highest in all cultivars, and its increase was paralled to the N levels. Magnesium and potassium were declined and calcium was increased with increased levels of nitrogen fertilizer. Ash content was decreased as nitrogen increased and as the plants have become matured. Three minutes pearling in Yonezawa was not sufficient in terms of water absorption ratio and whiteness. Sixty percent pearling rate in Yonezawa showed a higher absorption ratio and cooking whiteness than non-waxy Baekdong and Yeongsanbori. Gelatinization temperature was lower but maximum viscosity was higher in waxy than non-waxy barley.

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Studies on the Extraction of Seaweed Proteins Extraction of Water Soluble Proteins in Unexploited Seaweeds (해조단백질(海藻蛋白質) 추출(抽出)에 관(關)한 연구(硏究) -5. 적이용(赤利用) 해조(海藻)의 수용성(水溶性) 단백질(蛋白質) 추출조건(抽出條件)-)

  • Jeon, Yong-Hee;Lee, Kang-Ho;Ryu, Hong-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.9 no.1
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    • pp.15-22
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    • 1980
  • In this study, two species of algae, Ecklonia stolonifera, Sargassum thunbergii and one species of marine plant, Zostera marina(rhizoid and stem) were collected and extamined to determine the extractability of water soluble protein and the influences of various factors including extraction time, temperature, ratio of sample vs solvent and pH upon the extractability were tested. The effects of precipitation treatments for isolation of algal protein from the extracts(TCA treatment, methanol treatment and pH control) were also tested. Amino nitrogen and total nitrogen of purified samples made by obtained optimum conditions were estimated. The effect of the ratio of sample vs solvent on extractability differed from species to species which was enhances at 1:100(w/v) in Sargassum thunbergii and Zostera marina while 1:150(w/v) for Ecklonia stolonifera. The effect of extraction time and temperature was revealed differently in all species which might be considered to be caused by differences in the constitution of algal tissues. But in case of TCA insoluble nitrogen, it was showed the maximum extractability at $40-50^{\circ}C$ for 1 hour extraction. The optimum pH for the ext action of total nitrogen was 9-12 while the optimum pH was 6-7 for TCA insoluble nitrogen. And the pH control appeared to be most effective in the influence of precipitation treatment for isolation of algal protein.

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Studies on the Growth Characters and Nutrient Uptake Related to Source and Sink by Cool Water Temperature at Reproductive Growth Stage III. Influence of Growth Characters and Nutrient Uptake Related to Panicle by Different Water Temperature and Water Depth (생식생장기 냉수온이 벼의 Source와 Sink 관련형질 및 양분흡수에 관한 연구 III. 관개수온과 수심이 수의 관련제형질 및 양분흡수에 미치는 영향)

  • 최수일
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.2
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    • pp.242-248
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    • 1986
  • To study the effect of cold water irrigation at the reproductive growth stage of rice plants on several growth characters related to source and sink and nutrient uptake, the present experiment was carried out under the different conditions of water temperature and water depth. Deep irrigation with normal temperature water increased culm length, panicle length and panicle exsertion but with cold water resulted adversely. Most sensi-tive response in 5 cm water-depth appeared at reduction division stage and in 20 cm depth at panicle formation stage. Secondary branches and spikelets were increased in number by deep irrigation with normal temperature water, but decreased and degenerated by deep irrigation with cold water at panicle formation stage resulting in high spikelet sterility and low grain filling. Deep irrigation with normal temperature water increased the contents of total nitrogen, phosphate, potassium and silicate in leaf blades, branches and chaff. However, cold water irrigation reduced the uptake of phosphate, potassium and silicate except nitrogen particularly in deep irrigation. Ratios of phosphate, potassium and silicate to total nitrogen content were decreased by cold water irrigation. Branches seemed to have higher requirements for phosphate, potassium and silicate than leaf blades and chaff. Silicate-to-total nitrogen ratio in leaf blades, branches and chaff had significant correlations with yield showing closer relationship between yield and the ratio of silicate to total nitrogen in branches in particu-lar.

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Evaluation of Winter Green Manure Crops as Alternative of Expeller Cake Fertilizer on Environment-friendly Red Pepper Production (친환경 고추 재배에서 유박 비료 대체재로서 동계 풋거름 작물의 이용 가능성 평가)

  • Choi, Jang-Yong;Yun, Yeo-Uk;Lee, Jin-Il;Hong, Ki-Heung;Kang, Young-Sik
    • Korean Journal of Organic Agriculture
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    • v.30 no.2
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    • pp.279-290
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    • 2022
  • This study was conducted to find out that winter green manure crops could be efficient replacements of expeller cake fertilizers, which were mostly imported, on environment-friendly red pepper production. Four treatments were compared under the condition of plastic film house: 1) barley (B); 2) hairy vetch (HV); 3) mixtures of barley and hairy vetch (B/HV); 4) expeller cake (EC). Total nitrogen content in hariy vetch was 3.6%, which was higher than 1.5~1.8% in barley and mixtures of barley and hairy vetch. P2O5 and K2O contents were similar in all green manures. Supplying amount of nitrogen from B, HV and B/HV plot, which were 172 kg ha-1, 193 kg ha-1, and 198 kg ha-1, were higher than amount of basal nitrogen required by soil testing but were lower than that of total nitrogen, respectively. Among the green manure crops, C/N ratio of HV was the lowest at 11.8, showing a similar value to that of expeller cake, but that of B was the highest at 30.6. Total yield was no significant difference in all treatment plots although B/HV plot showed the highest yield, but initial yield in B plot with high C/N ratio was lower than that in EC plot.

Enhancing Electrical Properties of Sol-Gel Processed IGZO Thin-Film Transistors through Nitrogen Atmosphere Electron Beam Irradiation (질소분위기 전자빔 조사에 의한 졸-겔 IGZO 박막 트랜지스터의 전기적 특성 향상)

  • Jeeho Park;Young-Seok Song;Sukang Bae;Tae-Wook Kim
    • Journal of the Microelectronics and Packaging Society
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    • v.30 no.3
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    • pp.56-63
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    • 2023
  • In this paper, we studied the effect of electron beam irradiation on sol-gel indium-gallium-zinc oxide (IGZO) thin films under air and nitrogen atmosphere and carried out the electrical characterization of the s ol-gel IGZO thin film transistors (TFTs). To investigate the optical properties, crystalline structure and chemical state of the sol-gel IGZO thin films after electron beam irradiation, UV-Visible spectroscopy, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) were carried out. The sol-gel IGZO thin films exhibited over 80% transmittance in the visible range. The XRD analysis confirmed the amorphous nature of the sol-gel IGZO films regardless of electron beam irradiation. When electron beam irradiation was conducted in a nitrogen (N2) atmosphere, we observed an increased proportion of peaks related to M-O bonding contributed to the improved quality of the thin films. Sol-gel IGZO TFTs subjected to electron beam exposure in a nitrogen atmosphere exhibited enhanced electrical characteristics in terms of on/off ratio and electron mobility. In addition, the electrical parameters of the transistor (on/off ratio, threshold voltage, electron mobility, subthreshold swing) remained relatively stable over time, indicating that the electron beam exposure process in a nitrogen atmosphere could enhance the reliability of IGZO-based thin-film transistors in the fabrication of sol-gel processed TFTs.

Control of Microalgal Growth and Competition by N: P Ratio Manipulation (N: P ratio 조절에 의한 미세조류 생장과 경쟁 제어)

  • Ahn, Chi-Yong;Lee, Jae-Yon;Oh, Hee-Mock
    • Korean Journal of Environmental Biology
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    • v.31 no.2
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    • pp.61-68
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    • 2013
  • Microalgae can grow autotrophically with the supply of light, carbon dioxide and inorganic nutrients in water through photosynthesis. Generally, microalgal growth is limited by the concentrations and relative ratio of nitrogen (N) and phosphorus (P) among the nutrients in the aquatic environment. Each microalga has its specific optimum N : P ratio resulting in dominance in a particular water having similar nutrient composition. Algal bloom is an immense growth of certain microalga commonly cyanobacterium and can be sequestrated by reducing the limiting nutrient, generally P in the freshwater. Moreover, dominance of a less toxic blooming strain can be established by manipulating N : P ratio in the water. On the other hand, microalgal biomass of a certain species can be enhanced by increasing limiting nutrient and adjusting the N : P ratio to the target species. The above-mentioned eco-physiological features of microalgae can be more completely interpreted in connection with their genomic informations. Consequently, microalgal growth regulation which can be achieved on the basis of its eco-physiological and further genomic insights would be helpful not only in the control of algal bloom, but also for an increased yield of algal biomass.

Mineralization of Cattle Manure Compost at Various Soil Moisture Content (우분퇴비 시용후 토양수분 조절에 따른 질소 및 탄소의 전환)

  • Kim, P.J.;Chung, D.Y.;Chang, K.W.;Lee, B.L.
    • Korean Journal of Environmental Agriculture
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    • v.16 no.4
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    • pp.295-303
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    • 1997
  • To investigate the transformation characteristics of nitrogen and carbon from cow manure compost amended in soil under different moisture conditions, dynamics of nitrogen and carbon were determined periodically for 15 weeks of aerobic incubation at room temperature during July${\sim}$November, 1996. Cow manure compost matured with mixing saw dust was amended with the 4 ratios (0, 2, 4, 6%(wt/wt)) in Ap horizon soil, which collected from green house in Yesan, Chungnam. Moisture was controlled with 0.2, 0.3, 0.4, and 0.5 of mass water conte nt (${\theta}$m) to air dried soil, and water loss was compensated at every sampling. During incubation, soil pH was decreased continuously, that was caused by hydrogen generated from nitrification of ammonium nitrogen. And pH became higher with inclining cow manure compost amendment and water treatment, that meaned the increase of mineralization of organic-N to $NH_4\;^+-N$. Total nitrogen was reduced with increasing water content, but total carbon showed the contrast tendency with that of nitrogen. Therefore, C/N ratio slightly decreased in the low water condition (${\theta}$m 0.2) during incubation, but increased continuously in high water condition over ${\theta}$m 0.4. As a result, it was assumed that soil fertility is able to be reduced in the high water content over available water content. Nitrate transformation rate increased lasting in the low water content less than ${\theta}$m 0.3. Itdropped significantly in the first $2{\sim}3$ weeks of incubation over ${\theta}$m 0.4. In particular, nitrate was not detected in ${\theta}$m 0.5 of water content after the first $2{\sim}3$ weeks. In contrast, ammonium transformation was inclined with increasing water treatment. Nitrogen mineralization rate, which calculated with percentage ratio of (the sum of ex.$NH_4\;^+-N$ and $NO_3\;^--N$)/total nitrogen, was continuously increased in the low water content of ${\theta}$m 0.2 and 0.3. But it saw the different patterns in high water content over ${\theta}$m 0.4 that was drastically declined in the initial stage and then gradually inclined . From the above results, nitrogen transformation patterns differentiated decisively in water content between ${\theta}$m 0.3 and 0.4 in soil. Thus, it is very important for the maintain of suitable soil water content to enhance fertility of soil amended with manure compost. However, excess treatment of manure compost might enhance the possibility of contamination of small watershed and ground water around agricultural area.

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Effect of Amino Acids and Organic Nitrogen Sources on Cyclosporin A Fermentation by Tolypocladium inflatum (Tolypocladium inflatum을 이용한 Cyclosporin A 발효에서 아미노산과 유기질소원의 영향)

  • Kim, Jeong-Keun;Lee, Byung-Kyu;Chang, Seog-Won;Park, Yong-Deok;Rho, Yong-Taek
    • Microbiology and Biotechnology Letters
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    • v.37 no.2
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    • pp.140-146
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    • 2009
  • Cyclosporin, an immunosuppressant, is a representative group of biologically active secondary metabolites produced by the fungus Tolypocladium inflatum. The amount and ratio of cyclosporin derivatives in the culture broth are an important factors for the production of cyclosporin A and the purification in the industrial process. Therefore, we studied the effect of amino acids and complex organic nitrogen sources using Tolypocladium inflatum mutants on the productivity of cyclosporin A and the ratio of cyclosporin derivatives. Overproducing mutant YHC-004 having seven times higher productivity than mother strain's could be obtained through the artificial mutation by UV irradiation. The concentration and kind of organic nitrogens and amino acids shows the profound effect on the productivity of cyclosporin A and ratio of cyclosporin derivatives. As a result, it was possible to raise the productivity and the ratio of cyclosporin A up to 3,430 mg/L and 93% respectively, but on the other hand the other cyclosporin derivatives decreased less than 2% in the culture broth.