• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.121-121
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• 2022

When 7 kg·10a^-1, which is less than the nitrogen standard application amount of 9 kg·10a^-1, is applied, the protein content is lowered and the palatibility is improved. In order to examine the applicability of nitrogen fertilization of 7 kg·10a^-1 nationwide, soil samples were collected from 240 paddy fields in 8 provinces in 2021, and the organic matter content, effective phosphoric acid, and effective silicic acid were analyzed for each sample. As a result of one-way ANOVA analysis between samples collected for each province, there was no significant difference in the content of organic matter, effective phosphoric acid, and effective silicic acid except for some provinces. The contents of organic matter was higher than the appropriate level(25 ~ 30 g·kg^-1) except for Gyeongsangbuk-do, the effective phosphoric acid was higher than the appropriate level(80~120 mg·kg^-1) in all provinces, and the effective silicic acid was lower than the appropriate level(157 ~ 180 mg·kg^-1) except for Gyeonggi-do, Jeollanam-do and Gyeongsangnam-do. As a result of analyzing the recommended fertilization amount based on the nitrogen application amount of 7 kg·10a^-1, 68.3% ofthe 240 samples were able to give nitrogen fertilizer less than 7.5 kg·10a^-1, and the rest had to be given more than that to satisfy the standard fertilization amount. As a result of this study, 68.3% of rice paddies nationwide can be cultivated with a standard fertilization amount of 7 kg·10a^-1, however it was thought that continuous nutrient management would be required for other paddies.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.797-803
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• 2017

Objective: Effects of nitrogen supplementation associated with different levels of starch on voluntary intake, digestibility, and rumen and metabolic characteristics of cattle fed low-quality tropical forage (Brachiaria decumbens hay, 7.4% crude protein, CP) were evaluated using ruminal and abomasal cannulated steers. Methods: Five European${\times}$Zebu young bulls (186 kg body weight, BW) were distributed according to a $5{\times}5$ Latin square. The following treatments were evaluated: control, supplementation with 300 g CP/d (0:1), supplementation with 300 g starch/d and 300 g CP/d (1:1), supplementation with 600 g starch/d and 300 g CP/d (2:1), and supplementation with 900 g starch/d and 300 g CP/d (3:1). A mixture of nitrogenous compounds provided 1/3 from true protein (casein) and 2/3 from non-protein nitrogen (mixture of urea and ammonium sulphate, 9:1) was used as the nitrogen supplement. In order to supply energy a unique source of corn starch was used. Results: Supplements increased (p<0.05) dry matter intake, but did not affect (p>0.05) forage intake. There was a cubic effect (p<0.05) of starch on voluntary intake. This was attributed to the highest forage intake (g/kg BW) when using the 2:1 starch:CP ratio. Supplements increased (p<0.05) organic matter (OM) digestibility, but did not affect (p>0.05) neutral detergent fibre corrected for ash and protein (NDFap) digestibility. There was a positive linear effect (p<0.05) of the amount of starch supplemented on OM digestibility. Total NDFap digestibility was not affected (p>0.05) by the amount of supplemental starch. Ruminal ammonia nitrogen concentrations were higher (p<0.05) in supplemented animals, however, a negative linear effect (p<0.05) of amount of starch was observed. Supplements increased (p<0.05) the nitrogen balance (NB) and efficiency of nitrogen utilization. These effects were attributed to increased body anabolism, supported by higher (p<0.05) serum concentration of insulin-like growth factor 1. Increasing the amount of starch tended (p<0.06) to linearly increase the NB. In spite of this, there was a highest NB value for the 2:1 starch:CP ratio amongst the treatments with supplementation. Conclusion: Nitrogen supplementation in cattle fed low-quality tropical forage increases nitrogen retention in the animal's body. An additional supply of starch increases nitrogen retention by increasing energy availability for both rumen and animal metabolism.

• The Korean Journal of Ecology
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• v.19 no.2
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• pp.179-189
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• 1996

Growth and nitrogen retention of Persicaria thunbergii were investigated in the wetland microcosms which contained the plants growing on soil bed. Nitrogen solution was supplied to the microcosms with the same amount of $NH_4^{+}-N\; and\; NO_3^{-}-N$ at the rates of 0.00, 0.78, 1.57, 3.14g $N{\cdot}m^{-2}{\cdor}wk^{-1}$ from May 1 to August 31, 1995. The solution was detained for 5 days to react with soil and plant and then allowed to leach. The contents of NH_4^{+}-N\;and\; NO_3^{-}-N$ in the leachate, total Kjeldahl nitrogen, plant biomass, and soil characteristics were determined. Nitrogen retained by plant was estimated as the increment of TKN in plant biomass. The addition of 0.78 and 1.57g $N{\cdot}m^{-2}{\cdot}wk^{-1}$ resulted in significant increase of plant biomass. However, plant growth was inhibited when nitrogen was added at the rate of 3.14g $N{\cdot}m^{-2}{\cdot}wk^{-1}$. Overall, the plant biomass was positively correlated with the amount of nitrogen retained by plant and soil system. The amounts of $NO_3^{-}-N$ leached from the microcosms were 5~10 times higher than those of $NH_4^{+}-N$. While total nitrogen added ranged from 143.2 to 576.5g $N/m^2$, total leaching loss of inorganic nitrogen and nitrogen retained by plant was as little as 1.04~22.71g $N/m^2$, and 5.46~12.91g $N/m^2$, respectively. Then, the plant seemed to contribute to KDICical and microbial immobilization of nitrogen in the soil. Finally, it is suggested that a large portion of nitrogen added was lost into the air by denitrification and volatilizaton, and / or leached in organic forms.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.106-106
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• 2022

An appropriate amount of nitrogen fertilizer input during rice cultivation is essential for rice growth, quality control, and reduction of greenhouse gases in paddy fields. Therefore, it is necessary to develop a technology that can check whether an appropriate amount of fertilizer is applied in paddy fields. In this study, we tried to derive a method for diagnosing nitrogen fertilization level using spectroscopic diagnosis, physiological analysis, and molecular indicator genes. Nitrogen fertilization treatment was performed in a greenhouse by dividing into five treatment conditions: no fertilization (N0), low fertilization (N0.5), standard fertilization (N1.0), excessive fertilization (N1.5), and double fertilization (N2.0), respectively. Growth characteristics analysis was investigated by nitrogen fertilization conditions and growth stages, and the height of the canopy was analyzed using a laser scanner. Physiological and spectroscopic analyses were performed by analyzing chlorophyll and sugar contents and measuring SPAD and leaf spectrometer on rice leaves. In addition, real-time PCR experiment was performed to check the relative expression levels of several known nitrogen metabolism related genes. These results suggest that spectroscopic techniques can be helpful in diagnosing the level of nitrogen fertilization in rice paddy fields.

• Journal of Applied Biological Chemistry
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• v.42 no.1
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• pp.29-33
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• 1999

The present study examined annual runoff loading of nitrogen and phosphorus in the paddy field from 1 May, 1997 to 30 April, 1998. In the investigated area, the amount of rainfall was 1,095.6 mm and 414.6 mm during cropping season and non-cropping season. The annual rainfall was 1,510.2 mm. The total amount of runoff water was 1,043.2 mm and 281.0mm during cropping season and non-cropping season, and the added total amount of runoff water during two seasons was 1,324.2 mm. The runoff loading of nutrients caused by runoff water was measured as follows. The total-N was 149.23 and $8.67kg\;ha^{-1}$ (total amount=$157.90kg^{-1}ha^{-1}yr^{-1}$), the ammonia-N 102.98 and $4.44kg\;ha^{-1}$ ($107.42kg^{-1}ha^{-1}yr^{-1}$), the nitrate-N 28.45 and $1.23kg\;ha^{-1}$ ($29.68kg^{-1}ha^{-1}yr^{-1}$), the total-P 4.16 and $0.38kg\;ha^{-1}$ ($4.54kg^{-1}ha^{-1}yr^{-1}$) during cropping and non-cropping season respectively. When the loss ratio was calculated based on amounts of chemical fertilizer, about 68.6% of nitrogen and 16.7% of phosphorus was lost by runoff from applied fertilizer amount.

• Korean Journal of Soil Science and Fertilizer
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• v.4 no.2
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• pp.155-159
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• 1971

1. Based upon an fertilizer experiment done in a farmer's field, with a medium maturity variety, Jinheung, (in 1969) a method of determination of nitrogen to be additionally given, at tillering and reproductive stages was studied. In this experiment, four kilograms of nitrogen per 0.1 ha was applied as basal dressing to all experimental treatments at transplanting time. 2. The application of nitrogen at the reproductive stage of paddy increased the yield remarkably and cut down the amount of nitrogen to be given at the tillering stage. The yield of paddy, 670kg/0.1 ha obtained by the application of 6.82kg N/0.1 ha at the reproductive stage far exceeded the yield, 644kg/0.1ha obtained when over 8kg N/0.1ha of N is applied at tillering stage alone. This emphasizes the importance of nitrogen top dressing at the reproductive stage. 3. As the amount of nitrogen to be given at both stages, reproductive and tillering, would be the reflection of soil fertility of the field, a correlative study between the amount of nitrogen to be given at both stages and soil analysis is suggested to be undertaken. 4. A study on the relationship between the amount of fertilizer to be given at stages different growth and yield components is suggested to be undertaken for the purpose of establishing a better fertilizer use technique.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.176-182
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• 2022

The aim of this study was to investigate dry matter productivity and nitrate nitrogen content in the growth stages of barnyard millet (Echinochloa esculenta) cultivated for feed, which was treated with different nitrogen fertilization levels. An early variety of barnyard millet (cv. Shirohie) was used for the test, and the different treatments with nitrogen fertilizer were as follows: 50% (N-40 kg/ha, T1), 100% (N-80 kg/ha, reference amount, T2), 150% (N-120 kg/ha, T3), 200% (N- 160 kg/ha, T4), 250% (N-200 kg/ha, T5), and 300% (N-240 kg/ha, T6). Sowing was done on May 13, 2021 and plants were harvested for four stage; vegetative stage, elongation stage, heading stage, and milk stage. The length of the millet increased significantly as the amount of nitrogen fertilization increased during the harvest period (p<0.05), but the difference was insignificant during the milk stage (p>0.05). Moreover, barnyard millet dry matter yield increased significantly as the levels of nitrogen fertilization increased (p<0.05), but there was no significant difference in dry matter yield among nitrogen fertilization levels during the heading stage (p>0.05). Chlorophyll also was significantly higher in T5 (250%) at all harvesting times, whereas nitrate nitrogen content was highest at the vegetative stage, gradually decreased as growth progressed, and lowest at the milk stage. Finally, as the nitrogen fertilization levels increased, the nitrate nitrogen content was significantly higher in all treatment groups (p<0.05). Therefore, our results suggest that the most appropriate nitrogen fertilizer levels is between 150%-200%, considering the dry matter yield, feed ingredients and nitrate nitrogen content in barnyard millet for feed.

• Korean Journal of Microbiology
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• v.1 no.1
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• pp.19-25
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• 1963

The studies could be summarized as follows: 1. Standard Rf Values were made as Table II for the identification of amino acids. 2. Amounts of free amino nitrogen in eggs were increased about 0.03% (Table IV) while the amounts of total nitrogen (Table III) were increased lower percentage during the ten days of development. 3. Three unknown substances were found newly during the development. 4. Eight ninhydrine positive amino acids were present, in the fresh embryonic eggs, but the numbers increased to eighteen after fifty days of development. 5. Through the development, there were large amounts of glutamic acid, aspartic acid and alanine. 6. After fifty days development, the amount of amides, such as asparagine, glutamine and alanine were increased very large which were very small amount before it and the amount of asparagine were more than that of glutamine. 7. Tryptophane, Cystine , Histidine were detected after 50 days of development. This is very remarkable fact.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.3
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• pp.207-214
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• 2009

Crossover of nitrogen from cathode to anode is inevitable in typical membranes used in PEM fuel cells. This crossovered nitrogen normally accumulates in the hydrogen recirculation system at anode side channels. Excessive buildup of nitrogen in the anode side lowers the relative hydrogen concentration and finally affects the performance of fuel cell stack. So it is very important to analysis the nitrogen gas crossover at various operating conditions. In this study, characterization of nitrogen gas crossover in PEM fuel cell stack was investigated. The mass spectroscopy (MS) has been applied to measure the amount of the crossovered nitrogen gas at the anode exit. Results show that nitrogen gas crossover rate was affected by current density, anode and cathode stoichiometric ratio and operating pressure. Current density, anode stoichiometric ratio and anode operating pressure do not affect nitrogen crossover rate but anode exit concentration of nitrogen. Cathode pressure and stoichiometric ratio largely affect the nitrogen crossover rate.

• Journal of Biosystems Engineering
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• v.42 no.2
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• pp.98-103
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• 2017

Purpose: Precision agriculture includes determining the right amount of nitrogen for a specific location in the field. This work focused on developing and validating a model using variable rate nitrogen application based on the estimated SPAD value from the ground-based image sensor. Methods: A variable rate N application based on the decision making system was performed using a sensor-based variable rate nitrogen application system. To validate the nitrogen application decision making system based on the SPAD values, the developed N recommendation was compared with another conventional N recommendation. Results: Sensor-based variable rate nitrogen application was performed. The nitrogen deficiency level was measured using the image sensor system. Then, a variable rate application was run using the decision model and real-ti me control. Conclusions: These results would be useful for nitrogen management of corn in the field. The developed nitrogen application decision making system worked well, when considering the SPAD value estimation.