• Title/Summary/Keyword: nitrogen addition

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Measurement of Nitrogen Solubility with Ti addition in Stainless Steel (스테인레스강중의 Ti첨가에 따른 질소용해도 측정)

  • Lee, Young-Uk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.11
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    • pp.3043-3047
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    • 2009
  • Nitrogen solubility and nitride formation in liquid 304 Stainless steel has been measured by sampling method at various temperatures. Also values of thermodynamic functions are determined. Nitrogen solubility in molten 304 Stainless steel is increased with temperature and under the 1atm nitrogen atmosphere, titanium nitride is formed over 0.03wt%Ti in 304Stainless steel. Produced nitride is identified TiN by EDS analysis.

Production of Biomass and Lipid Using Microalga Nannochloris oculata Under Different Conditions of Nitrogen and Irradiance (미세조류 Nannochloris oculata의 성장과 지질 생산에 미치는 질소 농도와 광량의 영향)

  • Park, Sang-Jin;Choi, Yoon-E;Kim, Chul-Woong;Park, Won-Kun;Yang, Ji-Won
    • KSBB Journal
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    • v.25 no.6
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    • pp.553-558
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    • 2010
  • Increasing demands on fossil fuel have led to the unprecedented attraction to microalgal biofuel as an alternative energy. In this study, we investigated growth and lipid productions of microalga Nannochloris oculata under various carbon dioxide or nitrogen source concentrations and irradiance conditions. Biomass production of N. oculata was highest under 2% $CO_2$ with 0.3 flow rate (vvm). In addition, biomass productivities were proportional to the concentration of nitrogen source, whereas lipid biosynthesis was suppressed under higher nitrogen concentration (up to 50 mg/L). High irradiation ($160{\sim}180\;{\mu}mol/m^2{\cdot}s$) enhanced growth rate and lipid production of N. oculata.

Effect of waste cooking oil addition on ammonia emissions during the composting of dairy cattle manure

  • Kuroda, Kazutaka;Tanaka, Akihiro;Furuhashi, Kenichi;Fukuju, Naoki
    • Animal Bioscience
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    • v.35 no.7
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    • pp.1100-1108
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    • 2022
  • Objective: The objective of this study was to evaluate the effect of waste cooking oil (WCO) addition on ammonia (NH3) emissions during the composting of dairy cattle manure under two aeration conditions. Methods: The composting tests were conducted using the laboratory-scale composting apparatuses (14 L of inner volume). Three composting treatments (Control, WCO1.5, and WCO3, with WCO added at 0 wt%, 1.5 wt%, and 3 wt% of manure, respectively) were performed in two composting tests: aeration rate during composting was changed from 0.55 to 0.45 L/min in Test 1, and fixed at 0.3 L/min in Test 2, respectively. The NH3 emitted and nitrogen losses during the composting were analyzed, and the effect of the addition of WCO on NH3 emissions were evaluated. Results: Both tests indicated that the composting mixture temperature increased while the weight and water content decreased with increasing WCO content of the composting mixtures. On the other hand, the NH3 emissions and nitrogen loss trends observed during composting in Tests 1 and 2 were different from each other. In Test 1, NH3 emissions and nitrogen losses during composting increased with increasing WCO contents of the composting samples. Conversely, in Test 2, they decreased as the WCO contents of the samples increased. Conclusion: The WCO addition showed different effect on NH3 emissions during composting under two aeration conditions: the increase in WCO addition ratio increased the emissions under the higher aeration rate in Test 1, and it decreased the emissions under the lower aeration rate in Test 2. To obtain reduction of NH3 emissions by adding WCO with the addition ratio ≤3 wt% of the manure, aeration should be considered.

A Study on the Removal of Nitrogen and Phosphorus by Addition of Coagulant in the Sulfur Denitrification Process Coupled to the Membrane Bioreactor (MBR과 황탈질 공정에서 응집제 주입에 따른 질소.인 제거에 관한 연구)

  • Lee, Young-Ho;Yoo, Soung-Jong;Oh, Dae-Min;Lee, Young-Sin
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.10
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    • pp.949-956
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    • 2010
  • A method for simultaneous removal of nitrogen and phosphate from sewage by elemental sulfur denitrification with membrane bioreactor was proposed, and capacity $10\;m^3$/day of pilot plant was operated for 350 days. This study was investigated to have the effect of denitrification rate and T-P removal with the addition of Alum in Sulfur denitrification Reactor (SDR). The addition of Alum and alkalinity ($NaHCO_3$) in the effluent of MBR was tried to remove simultaneous phosphate and nitrogen in SDR. Characteristics of total nitrogen (T-N) and total phosphate (T-P) removal was compared without and with the addition of Alum as a coagulant. T-N removal without and with the addition of Alum was 92.1% and 87.8%, respectively. And denitrification efficiency was 93.8% and 87.1%, respectively. T-P removal rate was increased to 75.6% in SDR by addition of Alum (2.6~4 mg/L as Al), but T-P removal rate was about 26.7% without the addition of Alum. Therefore, denitrification rate was 6.7% of reduction but T-P removal rate was increased by addition of Alum.

Induction of Symbiosis between Nostoc muscorum and Cultured Plant Cells II. Changes of nitrogen fixation ability and morphology by association of N. muscorum with cultured tobacco cells (Nostoc muscorum과 식물배양세포의 공생유도에 관한 연구 II. N. muscorum과 담배배양세포의 혼합배양에 따른 질소고정능과 형태적 변화)

  • 정현숙
    • Journal of Plant Biology
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    • v.30 no.4
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    • pp.257-266
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    • 1987
  • Investigation on the ability of nitrogen usage by N2-fixing Nostoc muscorum and cultured tobacco cells when they were associately cultured on nitrogen-free media was carried out. Also, effect of polyamines on the associated cultured condition was carried out. In addition, morphological changes of N. muscorum and cultured tobacco cells in associate culture were observed to detect the possibility of induction of nitrogen fixing ability on cultued plant cells. The activity of nitrogenase increased markedly when N. muscorum was grown exclusively on nitrogen-free media. When N. muscorum was cultured associately with cultured tobacco cells on nitrogen-free media containing polyamines, high activity was detected in 10-4 M spermine treated group. Investigation on the change of polyamine amounts showed two times increase in spermidine and eight times increase in spermine on a associate culture. These effects of associated culture were shown through morphological change such as dense loclization of N. muscorum around the cultured tobacco cells as well as inside the cells. These results indicate the viability of N. muscorum in cultured tobacco cells and possible induction of nitrogen fixation ability by symbiosis.

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Roles of Azospirillum spp. Inoculation in Two Consecutive Growth of Maize Plants

  • Choi, Seung-Ju;Gadagi, Ravi;Park, Myung-Su;Yang, Jin-Chul;Sa, Tong-Min
    • Korean Journal of Environmental Agriculture
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    • v.21 no.1
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    • pp.74-79
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    • 2002
  • Two consecutive green house experiments were carried out to examine the effect of Azospirillum spp. inoculation on growth, nitrogen and phosphorus accumulation in maize plants grown in pots. There were eight treatments including an uninoculated control and Azospirillum strains OAD-3, OAD-9, AZ-22, AZ-8, AZ-9, Azospirillum brasilense BR-11001 and Azospirillum lipoferum BR-11080. The inoculated plants showed higher values in each of the following measurements; plant height, total dry mass and nitrogen and phosphorus accumulation in shoot when compared to the uninoculated control plants in two consecutive experiments conducted in the same soil. Among Azospirillum strains, Azospirillum sp. OAD-3 inoculated plants showed higher nitrogen accumulation by 44.5% and 45.1%, total dry mass by 48.6% and 66.9% in two consecutive experiments respectively. The nitrogen concentration in the maize plants was not changed significantly in the first experiment, however it increased significantly in the second experiment due to Azospirillum inoculation. In addition, Azospirillum sp. OAD-9 and A. brasilense BR-11001 also proved to be effective with respect to total dry mass, total nitrogen accumulation and total phosphorus accumulation. The nitrogen concentrations in maize plants were increased in the second experiment due to Azospirillum inoculation.

Hydrochemical Characteristics and Nitrate-Nitrogen Contamination in Shallow Groundwater in Two Agricultural areas in Korea

  • Sul-Min Yun;Hang-Tak Jeon;Ji-Min Hwang;MoonSu Kim;HyunKoo Kim;Se-Yeong Hamm
    • Journal of the Korean earth science society
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    • v.44 no.4
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    • pp.291-306
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    • 2023
  • Shallow groundwater in rural areas is primarily polluted by agricultural activities. Nitrate-nitrogen is an indicator of artificial pollution. In this study, the hydrochemical characteristics and nitrate-nitrogen pollution of shallow groundwater were examined in two agricultural villages (Hyogyo-ri and Sinan-ri) in Chungcheongnam-do Province, Korea. Physicochemical quality analysis of shallow groundwater and stream water in the field, and chemical analysis in the laboratory were conducted from July 2020 to October 2021. In Hygyo-ri and Sinan-ri villages, shallow groundwater mainly belonged to the Ca-Cl, Ca-H CO3, Na-HCO3, and Na-Cl types, whereas stream water predominantly belonged to the Ca-HCO3 type. The nitrate-nitrogen concentration in shallow groundwater varied depending on the season, displaying an increased concentration of nitrate-nitrogen in the dry season compared to the rainy season. Stream water may be influenced by runoff into villages from the surrounding area, although both shallow groundwater and stream water are affected by artificial pollution. In addition, the nitrate-nitrogen concentration in stream water was lower than that in shallow groundwater.

Physiological and Spectroscopic Changes of Rice by Nitrogen Fertilization Conditions

  • Jung-Il Cho;Dongwon Kwon;Hoejeong Jeong;Wan-Gyu Sang;Sungyul Chang;Jae-Kyeong Baek
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.106-106
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    • 2022
  • An appropriate amount of nitrogen fertilizer input during rice cultivation is essential for rice growth, quality control, and reduction of greenhouse gases in paddy fields. Therefore, it is necessary to develop a technology that can check whether an appropriate amount of fertilizer is applied in paddy fields. In this study, we tried to derive a method for diagnosing nitrogen fertilization level using spectroscopic diagnosis, physiological analysis, and molecular indicator genes. Nitrogen fertilization treatment was performed in a greenhouse by dividing into five treatment conditions: no fertilization (N0), low fertilization (N0.5), standard fertilization (N1.0), excessive fertilization (N1.5), and double fertilization (N2.0), respectively. Growth characteristics analysis was investigated by nitrogen fertilization conditions and growth stages, and the height of the canopy was analyzed using a laser scanner. Physiological and spectroscopic analyses were performed by analyzing chlorophyll and sugar contents and measuring SPAD and leaf spectrometer on rice leaves. In addition, real-time PCR experiment was performed to check the relative expression levels of several known nitrogen metabolism related genes. These results suggest that spectroscopic techniques can be helpful in diagnosing the level of nitrogen fertilization in rice paddy fields.

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Effect of Addition of Koji on Quality of Amino Acid Soysauce (국(麴)의 첨가가 아미노산간장의 품질에 미치는 영향)

  • Kim, Chan-Jo;Lee, Suk-Kun;Lee, Jong-Soo;Park, Chang-Hee
    • Applied Biological Chemistry
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    • v.31 no.2
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    • pp.154-161
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    • 1988
  • In order to improve the quality of amino acid soysauce, this experiment was carried out as follows; koji added amino acid soysauce(KAAS) was prepared by addition of defatted soybean and meal koji into amino acid soysauce containing $0.5%{\sim}1.5%$ of total nitrogen. Various physicochemical characteristics of KAAS during aging were determined. Total nitrogen, amino-nitrogen, specific gravity, pure extract, total acidity and color were high in KAAS at high total nitrogen. After one month aging, reducing sugar was high in KAAS at high total nitrogen, but pH was high in KAAS at low total nitrogen. Ethyl alcohol was 2.07% in KAAS at 0.5% of total nitrogen after five months aging and 1.4% in KAAS at 1.5% of total nitrogen. Levulinic acid and lactic acid were high in KAAS at low total nitrogen. The results obtained above summarized as the quality of KAAS were highly good at $0.5{\sim}0.7%$ of total nitrogen after two or three months aging.

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Treatment of stock wastewater by flocculation with Calsium and Magnesium salts (칼슘과 마그네슘염을 이용한 축산폐수의 응집처리)

  • 김재용
    • Journal of environmental and Sanitary engineering
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    • v.17 no.4
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    • pp.10-18
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    • 2002
  • The changes of conventional clarification processe and an increase in treatment cost are required to meet increasingly stringent regulations related to the treated water quality. Although many enhanced coagulations have introduced to improve organic matter removal, the results to remove color, nitrogen and phosphorus as well as organic material have not been very efficient yet. In this context as new flocculation using calcium hydroxide and magnesum sulfate was carried out. The removal of waste matters such as SS, organic matter, COD, nitrogen and phosphorus contained in stock wastewater was carried out by using the combination of calcium hydroxide and magnesium sulfate. The flocculation was investigated as a function of coagulant dose, pH, mixing time, settling time and coagulant addition modes such as the sequential addition of the two coagulants and the simultaneous addition of them. The flocculation by the combination of calcium hydroxide and magnesium sulfate was compared with that by aluminum sulfate. The mechanism of flocculation was investigated as well. About 60% of COD in stock watewater was removed by flocculation with combination of calcium hydroxide and magnesium sulfate.