• Title/Summary/Keyword: nitrogen absorption

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Characterization of Pigment-Producing Pseudoalteromonas spp. from Marine Habitats and Their Optimal Conditions for Pigment Production (해양환경에서 분리한 Pseudoalteromonas 속 균주들의 특징 및 색소 생성 최적 조건)

  • Jeong, Dong-Woo;Park, Jin-Sook
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1752-1757
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    • 2008
  • Three marine bacteria producing pigments were isolated from seawater of Jeju-Do and local solar saltern in Korea. Based on phenotypic characteristics and 16S rRNA sequence analysis, the strains were identified as Pseudoalteromonas spp., which produced red (Ju11-1), yellow (Ju14), and orange (TA20) pigments. The pigments showed UV absorption maxima at 537, 378 and 387 nm, respectively. The strains were growing well on Marine broth 2216 culture medium. The productivity of pigments reached the maximum value after 28 hours (Ju11-1, Ju14) and 24 hours (TA20) at $30^{\circ}C$, 2% NaCl and pH 6-7. The best pigment production of strains were supported by 1% of lactose (Ju11-1) and maltose (Ju14, TA20) as a carbon source and 1% of beef extract as a nitrogen source.

Effect of Chitin Application on the Early Growth of Tomato (토마토 초기 생장에 미치는 키틴 처리의 영향)

  • Jin, Yu-Lan;Ji, Myeong-Sim;Kim, Kil-Yong;Cha, Gyu-Suk;Chae, Dong-Hyun;Park, Ro-Dong
    • Applied Biological Chemistry
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    • v.47 no.3
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    • pp.361-365
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    • 2004
  • Chitin and its derivatives have been introduced to tomato seedlings by direct mixing of chitin, chitosan, or chitooligosaccharides (CO) with soils, watering of chitosan or CO solutions to soils, or chitosan-coating of tomato seeds as a biomodulator of plant growth, and the growth of tomato plants was measured 30- and 45-day after seeding in the pots. The treatment of chitin and its derivatives promoted the growth of tomato plants in plant heights, shoot weight, maximum leaf length and number of leaves. By comparison in the contents of cellular inorganic nutrients in the plants, it was found out that chitin or chitosan treatments accelerated nitrogen and potassium uptake but inhibit calcium uptake, suggesting that chitin and its derivatives modulate absorption of inorganic nutrients through plant roots from environment.

Decolorization and Biotransformation of Triphenylmethane Dye, Methyl Violet, by Aspergillus sp. Isolated from Ladakh, India

  • Kumar, C. Ganesh;Mongolla, Poornima;Basha, Anver;Joseph, Joveeta;Sarma, V.U.M.;Kamal, Ahmed
    • Journal of Microbiology and Biotechnology
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    • v.21 no.3
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    • pp.267-273
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    • 2011
  • Methyl violet, used extensively in the commercial textile industry and as a biological stain, is a hazardous recalcitrant. Aspergillus sp. strain CB-TKL-1 isolated from a water sample from Tsumoriri Lake, Karzok, Ladakh, India, was found to completely decolorize methyl violet within 24 h when cultured under aerobic conditions at $25^{\circ}C$. The rate of decolorization was determined by monitoring the decrease in the absorbance maxima of the dye by UV-visible spectroscopy. The decolorization of methyl violet was optimal at pH 5.5 and $30^{\circ}C$ when agitated at 200 rpm. Addition of glucose or arabinose (2%) as a carbon source and sodium nitrate or soyapeptone (0.2%) as a nitrogen source enhanced the decolorization ability of the culture. Furthermore, the culture exhibited a maximum decolorization rate of methyl violet after 24 h when the C:N ratio was 10. Nine N-demethylated decolorized products of methyl violet were identified based on UV-visible spectroscopy, Fourier transform infrared (FTIR), and LC-MS analyses. The decolorization of methyl violet at the end of 24 h generated mono-, di-, tri-, tetra-, penta-, and hexa-N-demethylated intermediates of pararosaniline. The variation of the relative absorption peaks in the decolorized sample indicated a linear decrease of hexa-N-demethylated compounds to non-N-demethylated pararosaniline, indicating a stepwise N-demethylation in the decolorization process.

Studies on the Nutrition of Amino Acids and Vitamins in Undaria pinnatifida (Harvey) Suringar (미역의 아미노酸 및 비타민에 對한 營養學的 硏究)

  • Lee, Hyun-Ki
    • Journal of the Korean Chemical Society
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    • v.9 no.4
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    • pp.201-210
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    • 1965
  • Leuconostoc mesenteroides P-60, Lactobacillus arabinosus 17-5, Streptococcus faecalis R have been successfully used for the quantitative determination of sixteen amino acids in Undaria pinnatifida (Harvey) Suringar hydrolysate by alkaline and hydrolysis for succesive two hours from two to twelve hours, by means of microbiological assay. And thiamine and riboflavin were fluorometrically determinated by thiochrome and lumiflavin in powder (80mesh) of Undaria pinnatifida (Harvey) Suringar. The results were as follows: 1) Arginine contents was the highest in hydrolysate for two hours, but longer the hydrolysis, the more content Undaria pinnatifida was decreased. 2) The adequate contents of other amino acids were obtained by hydrolysis for six hours. 3) Growth check and improve of Lactobacillus were not identified in determination by microbiological assay for Undaria pinnatifida. 4) The following values were obtained in Undaria pinnatifida hydrolysate six hours: asparatic acid 466, arginine 230, lysine 317, histidine 74, isoleucine 242, methionine 202, phenylalanine 256, proline 231, threonine 231, tyrosine 161, valine 415, glycine 302, leucine 414, glutamic acid 625, cystine (5 hrs.) 53 and tryptophan (8 hrs.) 90mg per nitrogen one gram. 5) Protein score was 81 (limiting factor was isoleucine) and essential amino acids pattern was of satisfactory results. And methionine contained was higher than FAO value or milk value. 6) Sulphur contained amino acids (methionine plus cystine) contained in Undaria pinnatifida were 225mg/N-g. That was satisfactory results. 7) Absorption spectrum of wave length were not different 1% HAc from buffer-sol. (pH 6.8) in dilution for determination of riboflavin. Both methods might be suitable. 8) Thiamine and riboflavin contained in Undaria pinnatifida were ($B_1,\;82.51{\pm}1.1){\gamma}/N-g\;and\;(B_2,\;115.29{\pm}1.5){\gamma}/N-g.$.

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Dynamic Rapid Synthesis of Bis(2,2'-bipyridine)nitrato Zinc (II) Nitrate Using a Microwave Method and its Application to Dye-Sensitized Solar Cells (DSSC)

  • Kim, Young-Mi;Kim, Su-Jung;Nahm, Kee-Pyung;Kang, Mi-Sook
    • Bulletin of the Korean Chemical Society
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    • v.31 no.10
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    • pp.2923-2928
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    • 2010
  • This study examined the synthesis of the crystal structure of bis(2,2'-bipyridine)nitrato zinc (II) nitrate, $[Zn(bipy)_2(NO_3)]^+NO_3^-$ using a microwave treatment at 300 W and 60 Hz for the application to dye-sensitized solar cells. The simulated complex structure of the complex was optimized with the density functional theory calculations for the UV-vis spectrum of the ground state using Gaussian 03 at the B3LYP/LANL2DZ level. The structure of the acquired complex was expected a penta-coordination with four nitrogen atoms of bipyridine and the oxygen bond of the $NO_3^-$ ion. The reflectance UV-vis absorption spectra exhibited two absorptions (L-L transfers) that were assigned to the transfers from the ligand ($\sigma$, $\pi$) of $NO_3$ to the ligand ($\sigma^*$, $\pi^*$) of pyridine at around 200 - 350 nm, and from the non-bonding orbital (n) of O in $NO_3$ to the p-orbital of pyridine at around 450 - 550 nm, respectively. The photoelectric efficiency was approximately 0.397% in the dye-sensitized solar cells with the nanometer-sized $TiO_2$ at an open-circuit voltage (Voc) of 0.39 V, a short-circuit current density (Jsc) of $1.79\;mA/cm^2$, and an incident light intensity of $100\;mW/cm^2$.

A Review on the Photochemical Oxidant Modeling as Applied to Air Quality Studies in Complex Terrain

  • Lee Hwa-Woon;Kim Yoo-Keun;Won Gyeong-Mee;Park Jong-Kil
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.1 no.1
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    • pp.19-33
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    • 1997
  • The high oxidants, which occur the daily maximum concentrations in the afternoon, are transported into the other region via long range transport mechanisms or trapped within the shallow mixing boundary layer and then removed physically (deposition, transport by mountain wind, etc.) and chemically (reaction with local sources). Therefore, modeling formation of photochemical oxidants requires a complex description of both chemical and meteorological processes. In this study, as a part of air quality studies, we reviewed various aspects of photochemical modeling on the basis of currently available literature. The result of the review shows that the model is based on a set of coupled continuity equations describing advection, diffusion, transport, deposition, chemistry, emission. Also photochemical oxidant models require a large amount of input data concerned with all aspects of the ozone life cycle. First, emission inventories of hydrocarbon and nitrogen oxides, with appropriate spatial and temporal resolution. Second, chemical and photochemical data allowing the quantitative description of the formation of ozone and other photochemically-generated secondary pollutants. Third, dry deposition mechanisms particularly for ozone, PAN and hydrogen peroxide to account for their removal by absorption on the ground, crops, natural vegetation, man-made and water surfaces. Finally, meteorological data describing the transport of primary pollutants away from their sources and of secondary pollutants towards the sensitive receptors where environmental damage may occur. In order to improve our present study, shortcomings and limitation of existing models are pointed out and verification process through observation is emphasized.

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Adiabatic Performance of Layered Insulating Materials for Bulk LH2 Storage Tanks (대용량 액체 수소 저장탱크를 위한 다층단열재의 단열성능 분석)

  • KIM, KYEONGHO;SHIN, DONGHWAN;KIM, YONGCHAN;KARNG, SARNG WOO
    • Transactions of the Korean hydrogen and new energy society
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    • v.27 no.6
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    • pp.642-650
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    • 2016
  • One of the most feasible solution for reducing the excessive energy consumption and carbon dioxide emission is usage of more efficient fuel such as hydrogen. As is well known, there are three viable technologies for storing hydrogen fuel: compressed gas, metal hydride absorption, and cryogenic liquid. In these technologies, the storage for liquid hydrogen has better energy density by weight than other storage methods. However, the cryogenic liquid storage has a significant disadvantage of boiling losses. That is, high performance of thermal insulation systems must be studied for reducing the boiling losses. This paper presents an experimental study on the effective thermal conductivities of the composite layered insulation with aerogel blankets($Cryogel^{(R)}$ Z and $Pyrogel^{(R)}$ XT-E) and Multi-layer insulation(MLI). The aerogel blankets are known as high porous materials and the good insulators within a soft vacuum range($10^{-3}{\sim}1$ Torr). Also, MLI is known as the best insulator within a high vacuum range(<$10^{-6}{\sim}10^{-3}$ Torr). A vertical axial cryogenic experimental apparatus was designed to investigate the thermal performance of the composite layered insulators under cryogenic conditions as well as consist of a cold mass tank, a heat absorber, annular vacuum space, and an insulators space. The composite insulators were laminated in the insulator space that height was 50 mm. In this study, the effective thermal conductivities of the materials were evaluated by measuring boil-off rate of liquid nitrogen and liquid argon in the cold mass tank.

Effect of dietary supplementation of fermented Rhus verniciflua on growth performance, apparent total tract digestibility, blood profile, and fecal microflora in weanling pigs

  • Balasubramanian, Balamuralikrishnan;Lee, Sang In;Shanmugam, Sureshkumar;Kathannan, Sankar;Lee, Il Seok;Kim, In Ho
    • Korean Journal of Agricultural Science
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    • v.44 no.1
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    • pp.67-76
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    • 2017
  • Fermented Rhus verniciflua (FRV) as feed additives act as excellent anti-diarrheal drugs as they increase the intestinal absorption rate therefore being indirectly associated with enhancing growth performance and increasing digestibility in livestock. A total of 80 weaned pigs with an average initial body weight (BW) of $6.82{\pm}1.11kg$ were used to evaluate a diet supplemented with FRV meal in a 6 week feeding trial with two dietary treatments [CON - basal diet; TRT - CON + 0.2% FRV] on growth performance, apparent total tract digestibility (ATTD), blood profiles, fecal microflora, and fecal score. Our results showed that the dietary supplementation of FRV improved (p < 0.05) average daily gain and gain : feed (G : F) ratio during days 15 - 42 and the overall experiment period and also increased the ATTD of dry matter (p < 0.05) at days 14 and 42. On the contrary, there was no effect (p > 0.05) on average daily feed intake, ATTD of nitrogen and energy, and blood profiles during the entire experiment. Moreover, dietary inclusion of FRV significantly increased fecal Lactobacillus (p < 0.05) counts and reduced the diarrhea during days 22 - 42. Thus, the results suggest that FRV can be used as a potential additive to improve growth performance and dry matter and to reduce diarrhea while having beneficial effects on fecal microflora in weanling pigs.

Ecological Studies on the Montane Grassland of Mt. Soback in Korea (소백산 산지초원의 생태학적 연구 II. 물질생산과 염류순환)

  • Kim, Joon Ho;Hyeong Tae Mun
    • The Korean Journal of Ecology
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    • v.5 no.4
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    • pp.204-210
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    • 1982
  • Comparative study of the biomass productioin and the cyclings of nitrogen, phosphorus and potassium was carried out on the east and the northwest facing slope in the montane grassland of Mt. Soback. The maximum productin during the growing season in the east and the northwest slope were 1, 150g/m2.yr, and 755g/m2.yr. in the August, respectively. The positive correlation was appearent between biomass production and A-layer depth of the soil and the correlation coefficient (r=.964) was very significant at 1% level. In the above ground materials, the nutrients contents were high at the early of the growing season but decreased gradually. In roots, however, there are no significant trend throughout the growing season. Total amounts of minerals uptaken by plants during the growing season in the east and the northwest site were 18.20 and 10.66g/m2.yr for N, 0.06 and 0.03g/m2.yr for P, 0.23 and 0.11g/m2.yr for K, respectively. the minerals returned to the soil by litter decomposition and roots decay in the east and the northwest site were 16.19 and 9.62g/m2.yr for N, 0.046 and 0.021g/m2.yr for P, 0.211 and 0.099g/m2.yr for K, respectively. The turnover the rate (absorbed/returned) of the nutrients in the east and the northwest site were 1.13 and 1.14 for N, 1.30 and 1.43 for P, 1.09 and 1.11 for K, and the absorption rates of minerals were 0.39 and 0.29% for N, 3.16 and 1.88% for P, 0.91 and 0.57% for K, respectively.

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Preparation of Cross-sectional Specimen for High Resolution Observation of Coating Structure and Visualization of Styrene/butadiene Latex Binder (고배율 도공층 구조 및 S/B latex 분포 분석을 위한 도공층 횡단면 제작)

  • Kim, Chae-Hoon;Youn, Hye-Jung;Lee, Hak-Lae
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.44 no.4
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    • pp.16-24
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    • 2012
  • To characterize the coating structure, diverse methods such as mercury intrusion, nitrogen adsorption and oil absorption methods have been developed and widely employed. These indirect techniques, however, have some limitation to explain the actual coating structure. Recently microscopic observation methods have been tried for analyzing structural characteristics of coating layers. Preparation of the undamaged cross section of a coating layer is essential for obtaining high quality image for analysis. In this study, distortion-free cross-section of the coating layer was prepared using a grinding and polishing technique. The coated paper was embedded in epoxy resin and cured. After curing the resin block it was ground with abrasive papers and then polished with diamond particle suspension and nylon cloth. Polished coating layer was sufficient enough to obtain undamaged cross sectional images with scanning electron microscope under backscattered electron image mode. In addition, the SEM images allowed distinction of the coating layer components. Also S/B latex film formed between pigment particles was visualized by osmium tetroxide staining. Pore size distribution and pore orientation were evaluated by image analysis from SEM cross-sectional images.