• 제목/요약/키워드: nitrite scavenging ratio

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해상죽 추출물의 성분 및 생리 활성에 관한 연구 (A study on chemical components and biological activities of bamboo extract)

  • 박승규;최두복;조훈
    • 환경위생공학
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    • 제23권2호
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    • pp.65-70
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    • 2008
  • The purposes of this study were to investigate the effects of bamboo (SasacoreanaNakai)extracton chemical characteristics and biological activities. pH ranges of bamboo extract using water and ethanol were between 5.2 and 6.1. The concentration of total phenol compounds using ethanol extract was 0.51%, which was about 2.0 fold higher than that of water extract. The mineral concentrations were order of K, Mg, Ca, and Na, respectively. The nitrite scavenging ratio of bamboo extract using ethanol was 20.4% in vitro. In the case of bamboo extract using water, it was 18.0%. Especially, the maximum nitrite scavenging ratio was obtained at pH 1.5. On the other hand, when pH was increased from 3.0 to 6.0, the nitrite scavenging ratio was decreased from 16.2% to 3.0%. The antioxidant activity of bamboo extract in vitro was increased from 100 to 160% when bamboo extract using ethanol was increased from 10 to $50{\mu}L$. These results suggest that the bamboo extract of Sasa coreana Nakai using ethanol can be used in bioactive and functional material.

볶음 처리 새송이버섯의 추출조건에 따른 생리기능적 특성 변화 (Change in Functional Properties by Extraction Condition of Roasted Pleurotus eryngii)

  • 윤성란;이명희;김현구;이기동
    • 한국식품영양과학회지
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    • 제35권3호
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    • pp.262-270
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    • 2006
  • 볶음 처리한 새송이버섯의 환류추출조건에 따른 추출물의 생리기능적 특성을 살펴보았다. 에탄올농도에 따라서는 50% 에탄올농도로 추출하였을 때 총 페놀성 화합물 함량 및 수소공여능이 높은 것으로 나타났으며, 75% 에탄올농도로 추출하였을 때 SOD유사활성이 높은 것으로 나타났다. 아질산염 소거능의 경우에는 에탄올농도가 낮을수록 높은 것으로 나타났다. 추출시간이 증가할수록 수소공여능은 감소하는 것으로 나타났으며, SOD유사활성 및 아질산염 소거능은 증가하는 것으로 나타났다. 추출온도가 증가할수록 수소공여능 및 SOD 유사활성은 감소하는 것으로 나타났으며, 아질산염 소거능은 큰 차이를 보이지 않았다. 용매에 대한 시료량이 증가할수록 수소공여능, SOD 유사활성 및 아질산염 소거능이 감소하는 것으로 나타났다. 이상의 결과를 바탕으로 하여 볶음 처리한 새송이버섯의 추출조건 설정에 활용될 수 있으리라 사료된다.

볶음전 증자처리가 둥굴레 근경의 수용성 성분 및 갈색화에 미치는 영향 (Effect of Steaming Prior to Roasting of Polygonatum odoratum Roots on Its Water Solubles and Browning)

  • 권중호;임종호
    • 한국식품저장유통학회지
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    • 제4권2호
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    • pp.155-162
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    • 1997
  • This work was designed to determine the effect of steaming-Pretreatment on Physicochemical and functional properties of roasted Polygonatum odoratum roots. Steaming treatments led to some reduction in the contents of water solubles, such as total solid, reducing sugar and free-amino nitrogen of the unroasted samples. Moreover, roasting processing caused decrease in reducing sugar and free-amino acid, with increase in total soluble solid and browning color of the samples, which showed the dependence of steaming treatment as well as roasting temperature. Electron-donating ability and nitrite-scavenging ratio of the samples were also highly developed along with roasting processing at 17$0^{\circ}C$ within 35min. Considering the physicochemical and some functional parameters of water extracts of roasted samples, roasting at above 14$0^{\circ}C$ for over 55min or at 17$0^{\circ}C$ for around 35min was recommendable for the higher quality of Polygonatum odoritum tea, which conditions were similarly adapted for both steamed and unsteamed samples.

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유자 용매추출물의 전자 공여능, 아질산염 소거능 및 NDMA 생성 억제능 (The Electron Donating Ability, Nitrite Scavenging Ability and NDMA Formation Effect of Solvent Extracts from Yuza (Citrus junos SIEB ex TANAKA))

  • 이수정;최선영;신정혜;서종권;임현철;성낙주
    • 한국식품위생안전성학회지
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    • 제20권4호
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    • pp.237-243
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    • 2005
  • 유자를 과육과 과피로 분리하여 methanol, ether, ethylacetate 및 물로 추출하며 전자 공여능, 아질산염 소거능 및 NDMA생성에 미치는 영향을 검색하였다. 유자 과육 추출물의 경우 10 mg/ml이상 첨가시 ether추출물을 제외한 모든 실험구에서 $50\%$이상의 전자 공여능을 나타내었다. 과피 메탄올 추출물을 40 mg/ml 첨가시에는 BHA 및 ascorbic acid의 효능과 유사한 수준인 $99.2{\pm}0.37\%$의 전자 공여능이 나타났다. pH 1.2에서 아질산염 소거작용은 1mg/ml 첨가시 $40\%$ 이상의 소거능을 보였으며 시료량이 증가할수록 상승하였다. 또한 과육보다는 과피에서 아질산염 소거능이 다소 우수한 것으로 나타났다. 유자 과육 추출물의 NDMA생성 억제 효과는 메탄을 추출물을 pH 1.2의 반응 용액에 40 mg/ml 첨가시 $31.7{\pm}1.25\%$로 가장 높게 나타났다. pH 4.2 및 6.0의 반응 조건에서는 모든 시료 첨가구에서 $20\%$미만의 억제 효과가 있었다.

복분자 추출조건에 따른 페놀성 화합물의 특성변화 (Changes in Phenolic Compounds Properties of Rubi Fructus Extract Depending on Extraction Conditions)

  • 윤성란;정용진;이기동;권중호
    • 한국식품영양과학회지
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    • 제32권3호
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    • pp.338-345
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    • 2003
  • 복분자 추출에 따른 페놀성화합물의 특성변화를 조사하기 위하여 반응표면분석법으로 시료에 대한 용매비(5, 10, 15 mL/g), 에탄을 농도(20, 40, 60%) 및 추출시간(2, 4, 6 hr)을 달리하여 가용성 고형분 함량, 총 페놀성 화합물 함량, 전자 공여능, 항산화능 및 아질산염소거능의 변화를 모니터링 하였다. 가용성 고형분 함량, 총 페놀성 화합물 함량, 전자공여능 및 항산화능은 시료에 대한 용매비의 영향을 많이 받는 것으로 나타났다. 아질산염 소거능의 경우 pH 1.2 및 6.0에서는 에탄을 농도의 영향을 많이 받았으며, pH 3.0및 4.2에서는 추출시 간의 영 향을 많이 받는 것으로 나타났다. 가용성 고형분 함량에 있어서 최적 추출조건은 시료에 대한 용매비 14.16mL/g, 에탄을 농도 39.08% 및 추출시간 2.99 hr으로 나타났으며, 총페놀성 화합물 함량의 경우 시료에 대한 용매비 14.06mL/g, 에탄을 농도 35.51% 및 추출시간 3.15 hr으로 나타났다

반응표면 분석에 의한 도토리 열수 추출물의 항산화적 특성 (Antioxidant Properties of Acorn Hot-Water Extract Using Response Surface Methodology)

  • 이진만;김성호
    • 한국식품저장유통학회지
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    • 제15권1호
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    • pp.111-117
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    • 2008
  • 본 연구에서는 도토리를 이용한 기능 식품개발을 목적으로 한 연구의 일환으로 도토리의 항산화 특성을 조사하기 위하여 추출온도 $30{\sim}70^{\circ}C$, 추출시간 $1{\sim}5$ 시간, 용매비 $5{\sim}25$ mL/g을 중심합성계획법으로 16개의 구간으로 하여 총페놀성 화합물의 함량, 전자공여능, 항산화능 및 아질산 염소거작용 등을 반응표면분석법으로 모니터링하여 최적추출조건을 설정하였다. 실험 결과 총페놀성 화합물 함량은 추출온도 $57.91^{\circ}C$, 추출시 간 4.08 시간 및 시료에 대한 용매비 22.39 mL/g에서 최대값을 나타내었으며, 용매비에 영향을 받고 있으며, 추출시간 및 추출온도의 영향은 없는 것으로 나타났다. 전자공여능에 대해서는 추출온도 $60.37^{\circ}C$, 추출시간 2.85 시간 및 용매비 6.47mL/g일 때 최대값을 나타내었으며, 항산화능에서는 최대값이 2.09 AI로 예측되었고, 이때 추출조건은 추출온도 $37.11^{\circ}C$, 추출시간 1.67시간 및 용매비 18.84 mL/g이었으며 추출온도, 추출시간 및 용매비 모두에 대해 영향을 크게 받고 있는 것으로 나타났다. 아질산염소거능은 추출온도 $47.07^{\circ}C$, 추출시간 1.24시간 및 용매비 19.55 mL/g에서 최대값을 나타내었다. 추출 후 아질산염소거능의 변화는 용매비에 영향을 가장 크게 받으며, 다음으로 추출온도에 영향을 받는 것으로 나타났으나 설정 범위내에서 추출시간에 대한 영향은 나타나지 않았다.

Effect of the Addition of Schisandra chinensis Powder on the Physico-chemical Characteristics of Sausage

  • Jin, S.K.;Park, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권12호
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    • pp.1753-1761
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    • 2013
  • The individual and interactive effects of Schisandra chinensis powder (SCP) and sodium nitrite additions on color, pH, water holding capacity, residual nitrite, 2-thiobarbituric acid reactive substances (TBARS), volatile basic nitrogen, texture properties, fatty acids, amino acids and sensory evaluation of cooked pork sausages were investigated after 20 d of storage at $4^{\circ}C$. The powders (0, 0.5 and 1.0%) were added to sausages either alone or in combination with nitrite (0 and 100 ppm). SCP added-sausages showed lower $L^*$ (lightness) and W (whiteness) values, and higher $b^*$ (yellowness) values than sausage containing no nitrite, and exhibited the highest $a^*$ values at a 0.5% addition (p<0.05). Residual nitrite and TBARS values were found to be significantly reduced as the addition levels of SCP increased (p<0.05). As the addition of SCP increased, the sausage showed gradually decreased brittleness, cohesiveness, springiness, gumminess and chewiness, while adhesiveness increased. Polyunsaturated fatty acid, n-6 and n-6/n-3 fatty acid ratio concentrations were significantly higher in sausages containing SCP (p<0.05). The addition of SCP to sausage significantly (p<0.05) increased the ammonia content (by 0.5% SCP) and aromatic amino acid concentrations (by 1.0% SCP) (p<0.05). Inclusion of SCP in sausage meat resulted in a significant deterioration in quality characteristics of flavor, springiness, juiciness and overall acceptability (p<0.05). As expected, the observed changes in $a^*$, W, pH, shear force, texture property, TBARS, fatty acid, amino acid and sensory score of sausages, depended on the rate of addition of nitrite (p<0.05). These results suggest that SCP addition is not an effective way of improving the sensory evaluation of sausages, but may beneficially affect TBARS, nitrite scavenging activity, fatty acid and amino acid content in pork sausages.

함초 씨앗의 화학적 특성과 아질산염 소거능 및 아세틸콜린에스터레이스 저해 효과 (Chemical Properties and Nitrite Scavenging and Acetylcholinesterase Inhibitory Activities from Salicornia herbaciea Seed)

  • 임금숙;김란;전경미;최현숙;조훈;고하영;최창남
    • KSBB Journal
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    • 제28권6호
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    • pp.372-379
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    • 2013
  • This study was to investigate the chemical properties and nitrite scavenging and acetylcholinesterase inhibitory activities from Salicornia herbaciea seed. The lactic acid content of seed was about 2.0 fold higher than that of stem. Among various free sugars, the maximum fructose of seed, glucose of stem, and mannose contents of root were obtained, 176.3, 125.6, and 112.8 mg/100g, respectively. The maximum leucine content of seed among the essential amino acid was obtained, 853.7 mg/100g, which was about 3.0 or 6.0 folds higher than that of root or stem. In the case of glutamic acid of seed, it was 2,388.7 mg/100g, which was 5.6 or 9.8 folds higher han that of root or stem. The ratio of essential amino acid and total amino acid of seed was 30.14%. The ${\gamma}$-aminobutyric acid contents of seed, stem, and root were 43.87, 23.88, and 27.8 mg/100g, respectively. The catechin content of seed was an order of epigallocatechin (723.2 mg/100g) > epigallocatechingallate (654.3 mg/100g) > epicatechin (443.5 mg/100g) > gallocatechin (314.1 mg/100g). Especially, the non-gallated catechins content was about 2.0 folds higher than that of gallated catechins content. The nitrite scavenging activity of seed increased from 38.7 to 65.9% when the hot-water extract content of seed at pH 1.2 increased from 1.0 to 5.0 mg/mL. However, it was decreased to 25.7% at pH 6. The acetylcholinesterase inhibitory activity of seed was increased from 13.2 to 44.6% when the extract content increased from 20 to 100 mg/mL. These results show that S. herbaciea seed has a good potential to be used as a source of material or additive in cosmetics, food, and drug compositions.

Taste Composition and Biological Activities of Cheonggukjang Containing Rubus coreanum

  • Kim, Eun-Jung;Hong, Ju-Yeon;Shin, Seung-Ryeul;Heo, Ho-Jin;Moon, Yong-Sun;Park, Sun-Hee;Kim, Kwang-Soo;Yoon, Kyung-Young
    • Food Science and Biotechnology
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    • 제17권4호
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    • pp.687-691
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    • 2008
  • This study was performed to investigate the taste composition and biological activities of cheonggukjang containing Rubus coreanum to improve cheonggukjangs' flavor and consumption. In R. corenum cheonggukjang (RCC), the total content of soluble sugars, including glucose, fructose, maltose, and sucrose, was 1,052.1 mg/100 g. Glutamic acid, phenlylalanine, leucine, cystine, and tyrosine were the major amino acids, and the ratio of sweet to bitter components was higher in RCC than in general cheonggukjang (GC). The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of the extracts, in decreasing order, were found to be: ethanol extract> water extract> chloroform extract, at all concentrations. The water extract had the highest SOD-like activity (10.2%) at a concentration of 0.5 mg/mL, whereas the chloroform extract showed the highest SOD-like activity (19.1%) at a concentration of 2 mg/mL. The nitrite scavenging ability was higher at pH 1.2 than at pH 3.0 or 6.0, and had a positive correlation with the extract concentration. The chloroform extract had the highest nitrite scavenging ability (84.6%) at a concentration of 2 mg/mL and pH 2.0.

Analysis of the Taste Components and Antioxidant Properties of Cheonggukjang Containing Korean Red Ginseng

  • Kim, Eun-Jung;Hong, Ju-Yeon;Shin, Seung-Ryeul;Moon, Yong-Sun;Yoon, Kyung-Young
    • Food Science and Biotechnology
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    • 제18권1호
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    • pp.53-59
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    • 2009
  • This study was performed to investigate the taste composition and antioxidant properties of cheonggukjang containing Korean red ginseng (RGC), as compared to either general cheonggukjang (GC) or non-fermented boiled soybeans (BS). Amylase activity was the highest (576.7 unit/g) in RGC, whereas protease activity was the highest (326.0 unit/g) in GC. The total soluble sugar contents of BS, GC, and RGC were 2,027.5, 905.5, and 837.5 mg/100 g, respectively. RGC had the highest amount of total amino acids (2,127.4 mg/100 g) and essential amino acid (50.9%) among the samples. The ratio of sweet to bitter components was higher in RGC than in GC. Although the extracts of RGC had higher radical scavenging activity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) than BS or GC, regardless of the extract concentration, the ethanol extract of RGC showed the highest scavenging ability (92.4%) at 2.0 mg/mL. The chloroform extracts from GC and RGC showed their greatest superoxide dimutase-like activities at 17.2 and 19.7% at a concentration of 2 mg/mL, respectively. Regardless of the samples, the nitrite scavenging ability was positively correlated to the extract concentration, and RGC had highest ability among samples under the same extract concentrations.