• Title/Summary/Keyword: next-generation method

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Diagnosis and Sequence Analysis of Japanese yam mosaic virus from Yam (Dioscorea opposita) (마(Dioscorea opposita)에 발생한 Japanese yam mosaic virus 진단 및 염기서열 분석)

  • Lee, Joong-Hwan;Son, Chang-Gi;Kwon, Joong-Bae;Nam, Hyo-Hun;Kim, Yeong-Tae;Kim, Mi Kyeong;Lee, Su-Heon
    • Research in Plant Disease
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    • v.22 no.4
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    • pp.289-292
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    • 2016
  • We surveyed the occurrence of Japanese yam mosaic virus (JYMV) on Yam in Gyeongsangbukdo pronvince from 2013 to 2015. The symptoms of JYMV were yellow stripes and chlorosis in yam leaves and the infection rate was ranged from 33.6% to 40.8%. We determined nucleotide sequence encoding the polyprotein of JYMV isolate BRI from yam leaves using next-generation sequencing (NGS) method. The partial nucleotide portion (7,736 nucleotides) of the genomic RNA of the JYMV isolate BRI has been sequenced (accession No. KU309315). The region sequenced includes a single open reading frame (ORF) encoding a polyprotein composed of 2,497 amino acids containing the coat protein (CP) and 3' untranslated region (UTR). The genomic organization of this isolate shows almost the same to that of other members of JYMV. The JYMV isolate BRI showed 77% to 79% nucleotide identity with the Japanese and Chinese strains and isolates. This is the first report of the genome nucleotide sequence of JYMV from Dioscorea opposita in Korea.

Production of Fermentable Sugar from Lipid Extracted Algae using Hot Water Pretreatment (열수전처리를 이용한 탈지미세조류로부터 발효당 생산 공정 개발)

  • Lee, Jihyun;Shin, Seulgi;Choi, Kanghoon;Jo, Jaemin;Kim, JinWoo
    • Korean Chemical Engineering Research
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    • v.54 no.4
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    • pp.443-447
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    • 2016
  • The microalgae have cellulose as a main structural component of their cell wall and the lignin content in microalgae is much lower than other lignocellulosic biomass. Therefore, fermentable sugar production from microalgae (Tetraselmis KCTC 12236BP) can be carried out under pretreatment without high temperature and high pressure. It was investigated that the effect of hot-water pretreatment using sulfuric acid for lipid extracted algae which is expected to be a next generation biomass. The effects of three major variables including extraction temperature, acid concentration and time on the enzymatic hydrolysis were investigated. Among the tested variables, temperature and acid concentration showed significant effects and optimum pretreatment conditions for the economic operation criteria were obtained as follows: reaction temperature of $120^{\circ}C$, sulfuric acid concentration of 2 mol and pretreatment time of 40 min. Under the optimum conditions of acidic hot water pretreatment, experimentally obtained hydrolysis yield were 95.9% which showed about 2.1 fold higher compared with enzymatic hydrolysis process. Therefore, acid pretreatment under mild condition was proven to be an effective method for fermentable sugar production from lipid extracted microalgae.

Comparison and Analysis of Name-Based Routing Protocols for Information-Centric Network (정보 중심 네트워크를 위한 이름 기반 라우팅 프로토콜의 비교 및 분석)

  • Kim, Jung-Jae;Ryu, Min-Woo;Cha, Si-Ho;Cho, Kuk-Huyn
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.4
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    • pp.1969-1975
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    • 2013
  • ICN (Information-Centric Network) is a next generation Internet communication technology for converting existing Internet communication paradigm to information-based communication paradigm to efficiently use a large amount of information that exists on the Internet. Therefore, unlike existing Internet communication technologies focused on the process of communication using the host address, ICN focuses on the purpose of communication for each information by defining the information of everything that exists on the Internet. For this purpose, ICN uses NbR (Name-based Routing) methods that assign a name to each piece of information, all routers participating in ICN have the physical storage so that they are able to share information with each other. NbR methods on ICN are divided into one-phase routing and two-phase routing depending on how to reach at the storage of each router. However, currently proposed NbR methods cause many problems because they do not reflect the unique characteristics of ICN. Therefore, this paper looked at various NbR issues from caching, access time, distribution, mobility, scaliability, and dissemination of information for an efficient NbR method, and analyzed existing methods proposed for ICN. This paper also proposed a research direction to study the efficient NbR for ICN based on the analysis information.

Prediction of Genomic Relationship Matrices using Single Nucleotide Polymorphisms in Hanwoo (한우의 유전체 표지인자 활용 개체 혈연관계 추정)

  • Lee, Deuk-Hwan;Cho, Chung-Il;Kim, Nae-Soo
    • Journal of Animal Science and Technology
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    • v.52 no.5
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    • pp.357-366
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    • 2010
  • The emergence of next-generation sequencing technologies has lead to application of new computational and statistical methodologies that allow incorporating genetic information from entire genomes of many individuals composing the population. For example, using single-nucleotide polymorphisms (SNP) obtained from whole genome amplification platforms such as the Ilummina BovineSNP50 chip, many researchers are actively engaged in the genetic evaluation of cattle livestock using whole genome relationship analyses. In this study, we estimated the genomic relationship matrix (GRM) and compared it with one computed using a pedigree relationship matrix (PRM) using a population of Hanwoo. This project is a preliminary study that will eventually include future work on genomic selection and prediction. Data used in this study were obtained from 187 blood samples consisting of the progeny of 20 young bulls collected after parentage testing from the Hanwoo improvement center, National Agriculture Cooperative Federation as well as 103 blood samples from the progeny of 12 proven bulls collected from farms around the Kyong-buk area in South Korea. The data set was divided into two cases for analysis. In the first case missing genotypes were included. In the second case missing genotypes were excluded. The effect of missing genotypes on the accuracy of genomic relationship estimation was investigated. Estimation of relationships using genomic information was also carried out chromosome by chromosome for whole genomic SNP markers based on the regression method using allele frequencies across loci. The average correlation coefficient and standard deviation between relationships using pedigree information and chromosomal genomic information using data which was verified using a parentage test andeliminated missing genotypes was $0.81{\pm}0.04$ and their correlation coefficient when using whole genomic information was 0.98, which was higher. Variation in relationships between non-inbred half sibs was $0.22{\pm}0.17$ on chromosomal and $0.22{\pm}0.04$ on whole genomic SNP markers. The variations were larger and unusual values were observed when non-parentage test data were included. So, relationship matrix by genomic information can be useful for genetic evaluation of animal breeding.

Feeding and Growth Rate of Earthworm(Eisenia fetida) Population on The Sewage Sludge Mixed and Aged with Food Waste Leachate or Paper Mill Sludge (하수슬러지와 음식물쓰레기 침출수슬러지 또는 제지슬러지를 혼합하여 부숙시킨 먹이에 대한 줄지렁이(Eisenia fetida)개천군의 섭식률 및 생장률)

  • Kim, Byung-Woo;Bae, Yoon-Hwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.16 no.3
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    • pp.52-64
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    • 2008
  • Aged sewage sludge with food waste leachate sludge or paper mill sludge was fed to earthworm (Eisenia fetida) populations and the effect of it on feeding and growth rate of earthworm population was investigated. Feeding rate of earthworm population on sewage sludge aged with food waste leachate sludge for 30 days was higher than that aged for 10 and 20 days. At 90 days after feeding, the density of earthworm population decreased regardless of aging period. And earthworm population growth to next generation was blocked by sewage sludge. The feeding rate and the density of earthworm population aged with paper mill sludge for 20 days were higher than that on sewage sludges aged for 10 and 30 days. Until 90 days after feeding, the density of earthworm population increased regardless of aging period of sewage sludge, however, since then decreased. There were comparatively higher feeding rate and growth rate of earthworm population on the sewage sludge which had been mixed with paper mill sludge and aged for 20 days, which rendered the probable pretreating method for vermicomposting sewage sludge.

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Rediscovery of haploid breeding in the genomics era (유전체 시대에 반수체 육종의 재발견)

  • Lee, Seulki;Kim, Jung Sun;Kang, Sang-Ho;Sohn, Seong-Han;Won, So Youn
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.12-20
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    • 2016
  • Advances in DNA sequencing technologies have contributed to revolutionary understanding of many fundamental biological processes. With unprecedented cost-effective and high-throughput sequencing, a single laboratory can afford to de novo sequence the whole genome for species of interest. In addition, population genetic studies have been remarkably accelerated by numerous molecular markers identified from unbiased genome-wide sequences of population samples. As sequencing technologies have evolved very rapidly, acquiring appropriate individual plants or populations is a major bottleneck in plant research considering the complex nature of plant genome, such as heterozygosity, repetitiveness, and polyploidy. This challenge could be overcome by the old but effective method known as haploid induction. Haploid plants containing half of their sporophytic chromosomes can be rapidly generated mainly by culturing gametophytic cells such as ovules or pollens. Subsequent chromosome doubling in haploid plants can generate stable doubled haploid (DH) with perfect homozygosity. Here, classical methodology to generate and identify haploid plants or DH are summarized. In addition, haploid induction by epigenetic regulation of centromeric histone is explained. Furthermore, the utilization of haploid plant in the genomics era is discussed in the aspect of genome sequencing project and population genetic studies.

Amorphous Indium-Tin-Zinc-Oxide (ITZO) Thin Film Transistors

  • Jo, Gwang-Min;Lee, Gi-Chang;Seong, Sang-Yun;Kim, Se-Yun;Kim, Jeong-Ju;Lee, Jun-Hyeong;Heo, Yeong-U
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.08a
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    • pp.170-170
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    • 2010
  • Thin-film transistors (TFT) have become the key components of electronic and optoelectronic devices. Most conventional thin-film field-effect transistors in display applications use an amorphous or polycrystal Si:H layer as the channel. This silicon layers are opaque in the visible range and severely restrict the amount of light detected by the observer due to its bandgap energy smaller than the visible light. Therefore, Si:H TFT devices reduce the efficiency of light transmittance and brightness. One method to increase the efficiency is to use the transparent oxides for the channel, electrode, and gate insulator. The development of transparent oxides for the components of thin-film field-effect transistors and the room-temperature fabrication with low voltage operations of the devices can offer the flexibility in designing the devices and contribute to the progress of next generation display technologies based on transparent displays and flexible displays. In this thesis, I report on the dc performance of transparent thin-film transistors using amorphous indium tin zinc oxides for an active layer. $SiO_2$ was employed as the gate dielectric oxide. The amorphous indium tin zinc oxides were deposited by RF magnetron sputtering. The carrier concentration of amorphous indium tin zinc oxides was controlled by oxygen pressure in the sputtering ambient. Devices are realized that display a threshold voltage of 4.17V and an on/off ration of ${\sim}10^9$ operated as an n-type enhancement mode with saturation mobility with $15.8\;cm^2/Vs$. In conclusion, the fabrication and characterization of thin-film transistors using amorphous indium tin zinc oxides for an active layer were reported. The devices were fabricated at room temperature by RF magnetron sputtering. The operation of the devices was an n-type enhancement mode with good saturation characteristics.

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A System Model of Iterative Image Reconstruction for High Sensitivity Collimator in SPECT (SPECT용 고민감도 콜리메이터를 위한 반복적 영상재구성방법의 시스템 모델 개발)

  • Bae, Seung-Bin;Lee, Hak-Jae;Kim, Young-Kwon;Kim, You-Hyun;Lee, Ki-Sung;Joung, Jin-Hun
    • Journal of radiological science and technology
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    • v.33 no.1
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    • pp.31-36
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    • 2010
  • Low energy high resolution (LEHR) collimator is the most widely used collimator in SPECT imaging. LEHR has an advantage in terms of image resolution but has a difficulty in acquiring high sensitivity due to the narrow hole size and long septa height. Throughput in SPECT can be improved by increasing counts per second with the use of high sensitivity collimators. The purpose of this study is to develop a system model in iterative image reconstruction to recover the resolution degradation caused by high sensitivity collimators with bigger hole size. We used fan-beam model instead of parallel-beam model for calculation of detection probabilities to accurately model the high sensitivity collimator with wider holes. In addition the weight factors were calculated and applied onto the probabilities as a function of incident angle of incoming photons and distance from source to the collimator surface. The proposed system model resulted in the equivalent performance with the same counts (i.e. in shortened acquisition time) and improved image quality in the same acquisition time. The proposed method can be effectively applied for resolution improvement of pixel collimator of next generation solid state detectors.

A Study on the Research Plan for R&D Long-term Load-map Design in the Facility Disaster on response of the changing Disaster Environment (재난환경변화에 대응한 인적재난 R&D중장기 로드맵 수립 기획연구)

  • Lee, Tae Shik;Seok, Geum Cheol;An, Jae Woo;Song, Cheol Ho;Cheung, Chong Soo;Lee, Young Jae;Cho, Won Cheol
    • Journal of Korean Society of Disaster and Security
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    • v.7 no.1
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    • pp.35-42
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    • 2014
  • This research has been shown the Research Plan for R&D Long-term Load-map Design in the Facility Disaster on response of the changing Disaster Environment, Showing the Strategy for the extending investment method of the technical development and related on that the advanced nation's technical level gap is minimized in the same area, Designing for the business' high-level, long-term load-map and the core driving strategy of the safety technology development business in the facility disaster, RFP Documentation on the core driving Business and the detailed driving Issue. This research has been resulted the three lists, trend analysis and Vision of the Technology Research Development (R&D) of the Safety Technology Area in domestic and foreign, the Responding Technology Predict and the Extended Research Strategy of the Next Generation Safety Technology in the facility Disaster, long-mid Term Road-Map of the Safety Technology in the facility Disaster, finally the Action Issue's RFP of the Safety Technology in the facility Disaster.

An Experimental Study on Blade Deformation of Coaxial Rotor System Using SPR(Stereo Pattern Recognition) Technique (SPR(Stereo Pattern Recognition) 기법을 이용한 동축 로터 블레이드의 변형에 대한 실험적 연구)

  • Yoo, Chanho;Yoon, Byung-Il;Chae, Sanghyun;Kim, Do-Hyung;Kim, Deog-Kwan
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.48 no.8
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    • pp.597-609
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    • 2020
  • These days, the coaxial rotor system is used for various purposes like UAVs, Mars exploration helicopters, and the next-generation high-speed rotorcraft. A number of research projects on aerodynamic performance of rotor systems, including the coaxial configuration have been made previously. On the contrary, research on rotor blade deformation has been mainly carried out regarding the single rotor system, where such effort has not been enough on the coaxial system. Nonetheless, in case of the coaxial system, blade deformation analysis is much more important because of the complex air flow around the rotors, and that the distance between the two rotors is a key factor affects aerodynamic performance of the entire system. For these reasons, an experimental study on rotor blade deformation of the coaxial system was conducted using the Stereo Pattern Recognition(SPR) technique, one of the state-of-the-art of photogrammetry method. In this research, a small-scale coaxial rotor test stand designed by Korea Aerospace Research Institute(KARI) was used. With the same test stand, performance of the coaxial configuration had been studied before the experimental study on blade deformation, in order to find the relation between performance and blade deformation of the rotor system. Results of the performance test and the deformation study are presented in this article.