• Journal of Microbiology and Biotechnology
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• v.13 no.2
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• pp.182-190
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• 2003

A new phaC gene cluster encoding polyhydroxyalkanoate (PHA) synthase I PHA depolymerase, and PHA synthase II was cloned using the touchdown PCR method, from medium-chain length (mcl-) PHA-producing strain Pseudomonas putida KT2440. The molecular structure of the cloned phaCl gene was analyzed, and the phylogenic relationship was compared with other phaCl genes cloned from Pseudomonas species. The cloned phaCl gene was expressed in a recombinant E. coli to the similar level of PHA synthase in the parent strain P. putida KT2440, but no significant amount of mcl-PHA was accumulated. The isolated phaCl gene was re-introduced into the parent strain P. putida KT2440 to amplify the PHA synthase I activity, and the recombinant P. purida accumulated mcl-PHA more effectively, increasing from 26.6 to $43.5\%$. The monomer compositions of 3-hydroxylalkanoates in mcl-PHA were also modified significantly in the recombinant P. putida enforcing the cloned phaCl gene.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.2
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• pp.239-246
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• 2003

This paper proposes engineering estimation equations of elastic-plastic J and COD fur axial through-wall cracked pipes under internal pressure. Based on detailed 3-D FE results based on deformation plasticity, the plastic influence functions for fully plastic J and COD solutions are tabulated as a function of the mean radius-to-thickness ratio, the normalized crack length. and the strain hardening. Based on these results, the GE/EPRI-type J and COD estimation equations are proposed and validated against the 3-D FE results based on deformation plasticity. For more general application to general stress-strain laws or to complex loading, the developed GE/EPRI-type solutions are re-formulated based on the reference stress concept. Such a reformulation provides simpler equations for J and COD, which are then further extended to combined internal pressure and bending. The proposed reference stress based J and COD estimation equations are compared with elastic-plastic 3-D FE results using actual stress-strain data for Type 316 stainless steels. The FE results for both internal pressure cases and combined internal pressure and bending cases compare very well with the proposed J and COD estimations.

• Journal of Microbiology and Biotechnology
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• v.12 no.5
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• pp.746-752
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• 2002

The purpose of the present study was to examine the antagonistic activities of Enterococcus faecalis strain PL9003 (PL9003) on Helicobacter pylori. This strain was isolated from infant feces and found to inhibit both the growth of H. pylori and its in vitro adherence to the human gastric cell line MKN-45. The binding of PL9003 to MKN-45 was observed under a light microscope after Cram staining and under a scanning electron microscope. When detected with an FITC-conjugate antibody, both viable and nonviable PL9003 were found to decrease the number of H. pylori bound to MKN-45. When detected by an enzyme-linked immunoabsorbent assay, about 70% of the H. pylori bound on MKN-45 disappeared with the four-1314 addition of viable or nonviable PL9003. The spent culture supernatant (SCS) of PL9003 also decreased the viability of H pylori even after neutralization and pepsin treatment. The above results suggest that PL9003 has a potential as a new probiotic for the stomach.

• Transactions of Materials Processing
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• v.18 no.6
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• pp.453-457
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• 2009

In this paper, plastic deformation behaviors of ESW105 and SCM435 steels are revealed by simulations and experiments. ESW105 is the special pre-heat-treated steel characterized by high initial yield strength and negligible strain-hardening behavior. The flow stresses of the two steels for large stain are calculated from tensile tests. Axial and lateral compressions of cylindrical bars are tested and simulated and the deformed shapes are compared to characterize the plastic deformation behaviors of the two materials. A forward extrusion process of a cylindrical bar is also simulated to reveal the difference. It has been shown that there are pretty much difference in plastic flow between ESW105 and SCM435 which causes from the difference in strain-hardening capability, implying that the experience-oriented design rules for common commercial materials may lead to failure in process design when the new material of ESW105 is applied without consideration of its plastic deformation behavior.

• Smart Structures and Systems
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• v.17 no.4
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• pp.541-557
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• 2016

Prefabricated bridge substructures provide new possibility for designers in terms of efficiency of creativity, fast construction, geometry control and cost. Even though prefabricated bridge columns are widely adopted as a substructure system in the bridge construction project recently, lack of deeper understanding of the seismic behavior of prefabricated bridge substructures cause much concern on their performance in high seismic zones. In this paper, experimental research works are presented to verify enhanced design concepts of prefabricated bridge piers. Integration of precast segments was done with continuity of axial prestressing tendons and mild reinforcing bars throughout the construction joints. Cyclic tests were conducted to investigate the effects of the design parameters on seismic performance. An analytical method for moment-curvature analysis of prefabricated bridge columns is conducted in this study. The method is validated through comparison with experimental results and the fiber model analysis. A parametric study is conducted to observe the seismic behavior of prefabricated bridge columns using the analytical study based on strain compatibility method. The effects of continuity of axial steel and tendon, and initial prestressing level on the load-displacement response characteristics, i.e., the strain of axial mild steels and posttensioned tendon at fracture and concrete crushing strain at the extreme compression fiber are investigated. The analytical study shows the layout of axial mild steels and posttensioned tendons in this experiment is the optimized arrangement for seismic performance.

• Nuclear Engineering and Technology
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• v.32 no.1
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• pp.77-87
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• 2000

It is reported that the toughness and safety margins of the SA106 Gr.C main steam line piping steel is reduced due to dynamic strain aging (DSA) at the reactor operating temperature for Leak-Before-Break (LBB) application. In this study, intercritical annealing in two-phase ($\alpha$+${\gamma}$）region was performed to investigate the possibility of improving the toughness and reducing DSA susceptibility. The manifestations of DSA were still observed in the tensile tests of the annealed specimens. However, the ductility loss caused by DSA was smaller than that in the as-received material. Furthermore, the intercritical annealing was able to increase the Charpy impact toughness by 1.5 times compared to as-received. With the heat treatment, we could obtain microstructural changes such as the cleaner retained ferrite, increased ferrite content and somewhat finer grain size. It is considered that the reduced DSA was induced by cleaner retained ferrite, which in turn resulted in higher impact toughness in addition to the general toughening due to finer grain sizes and increased ferrite content.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.05a
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• pp.48-51
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• 2009

In this paper, plastic deformation behaviors of ESW105 and SCM435 steels are revealed by simulations and experiments. ESW105 is the special pre-heat-treated steel characterized by high initial yield strength and negligible strain-hardening behavior. The flow stresses of the two steels for large stain are calculated from tensile tests. Axial and lateral compressions of cylindrical bars are tested and simulated and the deformed shapes are compared to characterize the plastic deformation behaviors of the two materials. A forward extrusion process of a cylindrical bar is also simulated to reveal the difference. It has been shown that there are pretty much difference in plastic flow between ESW105 and SCM435 which causes from the difference in strain-hardening capability, implying that the experience-oriented design rules for common commercial materials may lead to failure in process design when the new material of ESW105 is applied without consideration of its plastic deformation behavior.

• Microbiology and Biotechnology Letters
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• v.19 no.3
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• pp.235-241
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• 1991

- A phthalic acid derivative and basic macrolide antibiotics, with antimicrobial activity against Gram positive bacteria and phytopathogenic fungi, respectively, were found to be produced by a strain 88-GT-161 identified as being a variety of Streptomyces melanosporofaciens. This paper describes an isolation procedure of the active compounds produced by this strain, their in vitro and in vivo (pot test) antimicrobial activites, and structure determination of one of the compounds, bis (2-ethylhexyl) phthalate, a phthalic acid derivative antibiotic. This compounds, upon cornparision with authentic bis (2-ethylhexyl) phthalate, dioctyl phthalate, revealed a difference in antimicrobial activity even though physico-chemical properties of these two compounds seemed indentical. This is the first report that dioctyl phthalate is biosynthetically produced by a Streptomyces sp. and shows antimicrobial activity.

• Proceedings of the Korea Concrete Institute Conference
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• 2000.10b
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• pp.995-1000
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• 2000

Segmentally erected prestressed concrete box girder bridges have been widely used in Korean high speed railway. Segmental erection has been accomplished along the longitudinal direction and across the depth of cross section. The cross section is similar to a composite cross section, composed of old and new segments. Because these segments have different time-dependent creep and shrinkage properties, a stress redistribution takes place during the construction period. It is the main objective in this research to investigate this behavior. An actual bridge was instrumented with 96 vibrating wire embedded type strain gauges, 6 electronic type steel strain gauges, and 75 thermocouples. Two span continuous high speed railway bridge was selected. Two points of importance, such as the midpoint of the first span and the point of interior support, along the span of the girder were chosen to monitor the time dependent behaviors for an extended period of time. The data collection was starting just after concrete girder were cast and is still going on. According to the measured results, the strain distributions across the depth of the section at midspan and interior support were not continuous and the important redistribution of stresses takes place. Thus, rational design of prestressed concrete composite box girder bridges need.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.1693-1706
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• 2008

It is of importance to determine the zero effective stress void ratio($e_{00}$), which is the void ratio at effective stress equal to zero, and the relationships of void ratio-effective stress and of void ratio-hydraulic conductivity for characterizing non-liner finite strain consolidation behavior for ultra-soft dredged materials. The zero effective stress void ratio means a transitional status from sedimentation to self-weight consolidation of very soft soil deposits, and acts as a starting point for self-weight consolidation in the non-linear finite strain numerical analysis such as PSDDF. In this paper, a new method for determining the zero effective stress void ratio has been introduced with the aid of measuring electrical resistivity of the specimen. A correlation between the zero effective stress void ratio and the initial slurry void ratio has been proposed, which can be used in PSDDF analysis as an input parameter. Combining all of the accessible experimental data, the consolidation characteristics of a dredged soil from the Incheon area has been studied in detail.