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New Species and Eight Undescribed Species Belonging to the Families Aspergillaceae and Trichocomaceae in Korea

  • Nguyen, Thuong T.T.;Noh, Kyo Jang Kwan;Lee, Hyang Burm
    • Mycobiology
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    • v.49 no.6
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    • pp.534-550
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    • 2021
  • During a survey of fungal diversity associated with insects, mud, soil, and freshwater niches in different areas in Korea, nine interesting fungal strains were isolated. Based on their morphological characteristics and molecular phylogeny analyses, using a combined data set of b-tubulin (BenA), calmodulin (CaM), and second largest subunit of RNA polymerase (RPB2) sequences, the strains CNUFC AM-44, CNUFC JCW3-4, CNUFC S708, CNUFC WT202, CNUFC AS1-29, CNUFC JCW3-5, CNUFC JDP37, and CNUFC JDP62 were identified as Aspergillus alabamensis, A. floridensis, A. subversicolor, Penicillium flavigenum, P. laevigatum, P. lenticrescens, Talaromyces adpressus, and T. beijingensis, respectively. The strain CNUFC JT1301 belongs to series Westlingiorum in section Citrina and is phylogenetically related to P. manginii. However, slow growth when cultivated on CYA, MEA, CREA is observed and the property can be used to easily distinguish the new species from these species. Additionally, P. manginii is known to produce sclerotia, while CNUFC JT1301 strain does not. Herein, the new fungal species is proposed as P. aquadulcis sp. nov. Eight species, A. alabamensis, A. floridensis, A. subversicolor, P. flavigenum, P. laevigatum, P. lenticrescens, T. adpressus, and T. beijingensis, have not been previously reported in Korea. The present study expands the known distribution of fungal species belonging to the families Aspergillaceae and Trichocomaceae in Korea.

Caregiver Burden and Rewards in New Generation and Middle-aged Couples (신세대와 중년기 부부의 노부모 부양에 따른 부담감 및 보상감 분석)

  • 안선영
    • Journal of Families and Better Life
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    • v.14 no.2
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    • pp.97-110
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    • 1996
  • The purpose of this study was to compare the caregiver burden and rewards in new generation and middle-aged couples. Data were collected from 207 new generation couples and 196 middle-aged couples who had either of older parents and lived in Seoul. Major findings are as fallows; 1. The burden were constituted with restriction of privacy physical burden strain psychological burden economic cost and guilt. The rewards were constituted with recognition pride maturity improving relationship and assistance of household and childcare. 2. The caregiver burden of new generation and middle-aged wives were hgiher than husbands. Hwever the caregiver rewards of new generation and middle-aged husbands were higher than wives. 3. The caregiver burden of middle-aged husbands were higher than new generation husbands bur there was no significant difference between new generation and middle-aged wives. 4. The caregiver rewards of middle-aged wives were higher than new generation wives but there was no significant difference between new generation and middle-aged husbands.

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An Investigation of the Control of Two-Spotted Spider Mites (Tetranychus urticae Koch) Resistant to Organo-Phosphates (유기인제저항성 점박이응애(Tetranychus urticae Koch)에 관한 연구)

  • Lee Seung Chan;Harrison R. A.
    • Korean journal of applied entomology
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    • v.7
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    • pp.39-51
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    • 1969
  • The study involved determination of resistance levels of spider mites ta argano-phosphates using topical application and slide dip techniques; laboratory serening tests of alternative acaricides using an O/P resistant strain and a field trial of the screened materials. 1. Strains of Tetranychus were from Timaru(TR), Havelock Narth (HNR), Lincaln (LN). Germany (GR, GN). Comparisons of the resistant strains and normal strains at the LD50 and LC50 levels were as follows : (a) Using the topical application tochnique; with Parathian. resistant levels of the GR. TR and HNR strains of T. urticae were respeativuly, 1035. 484 and 452 times as resistant' as the LN strain. (b) Using the slide dip technique; with Phosdrin, resistant of GR, TR and HNR strains of T. urticae were 635, 274 and 266 times greater respeativuly, than the GN strain. 2. The laboratory sereaning tests were carried out far their contact plus stomach and residual effect to assess the toxicities of eleven alternative materials which would be used far control of O/P resistant strain of T. urticae. The acaricide groups represented were 3 organo-chlorines (Spidex, Kelthane and C 8514), 2 nitrophenyls (UC 19786 and Morocide), 2 cyclic carbonates(Eradex and Morestan). I carbamate (UC2004 7A), 1 mixture of carbamate and orano-chlorine and 2 other chemicals (C 8677 and M2527). From all acaricide tested. Kelthane and Morocide were the most effective, folowed by Spidex and M2527. Morestan, C8514. C8677 and RS 143 were intermediate, but Eradex, UC 19786 and UC 20046A were poor. 3, The number of sapmles required for estimation of the population in the field evaluation of acaricidal effects was one giving the highest practical precision. It was decided, after preliminary sampling trials. to use samples of 30 leaves per replicate which gave a $5.7\%$ standard error. 4. In the field trials, Morocide applied at the $0.05\%\;and\; 0.04\%$ a. i. conc. to black currant trees gave excellent control of O/P resistant population of T. urticae for about 12 days, but Morocide 0.025 and Kel thane $0.02\%$ a. i. cone. gave efficient control for about 6 days. In other words. first applications of Kel thane ane Moroeide gave very high degrees of control of O/P resistant population of the two-spotted spider mite. However, the results indicate that secondary application would sometimes be necessary. There was no foliage damage of black Currants and strawberries by either acaricides at the concentrations used. Acknowledgment ... The authors are grateful to: Dr. R. P. pottinger, Senior Lecturer in Agricultural Zoology. Lincoln college. New Zealand. for his helpful assistance in aiding with the organization of thd field work. Department of agriculture officers for mite colonies. Mr. D. A. Slade, Technical Advisor. Fruitgrowers' Federation (now at Massey University) for his assistance and provision of mites for testing. Mr T. McRae of Timaru for permission to use his crops for field tests. The following chemical companies and I or their New Zealand agents for so readily supplying samples of acarides; Ivan Watkins-Dow Limited. Fruitgrowers Chemical Company Limited. Henry H. York & company (New Zealand). Shell Oil (New Zealand) Limited.

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Development on Steel Pipe for Hydroforming by Roll Forming Analysis (롤 성형 해석을 통한 하이드로포밍 전용 강관 개발)

  • 이봉열;조종래;문영훈;송병호;박중호
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2003.10a
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    • pp.229-232
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    • 2003
  • In the roll forming process, a sheet or strip of metal is continuously and progressively formed into a desired cross-sectional profile by feeding it through a series of forming roll. Accordingly, it is important to maintain the material properties of the initial sheet and deform uniformly during the roll forming. The roll forming process was estimated in consideration of some factors such as material properties, strip thickness, roll diameter, roll velocity, and the deformation of the material that influence the forming length. The hydroforming technology has been recognized as a new technique in manufacturing industry, especially in automotive industry. The formed pipe in used in hydroforming process is manufactured by the roll forming. The formability during hydroforming is very sensitive to the state of pipes which are made by roll forming. Particularly the amount of hardening during roll forming affects the formability. Therefore, it is necessary to design the optimum roll flower to reduce the local hardening. In this paper, optimum roll flower which has uniform strain distribution through sheet width was obtained by comparing strain distribution in various roll flower. Finite element analysis(FEA) is performed to estimate the strain distribution related to hardening by roll forming. A numerical analysis is carried out by SHAPE-RF.

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Antibiotics produced by anaerobic fermentation of Streptococcus sp. An-21-1 isolated from domestic soil I. Screening and identification of anaerobic bacteria (국내토양에서 분리한 혐기성 세균 Streptococcus sp. An-21-1 이 생성하는 항생물질 I. 혐기성 세균의 선별과 동정)

  • Park, Seung-chun;Yun, Hyo-in;Oh, Tae-kwang
    • Korean Journal of Veterinary Research
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    • v.33 no.1
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    • pp.53-60
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    • 1993
  • Anaerobic bacteria are suggested to be potential source for new antibiotics. In order to search for antibiotics from domestic origin, we collected 800 soil samples across Korean locations and could isolate as many as 989 anaerobic strains. Among them 10, strains were found to have good producing capacity of antibiotics. An anaerobe was finally selected due to secreting antibiotics having high antimicrobial activity towards multiple resistant microorganism(E coli JM 83) transformed by genetic engineering technique. Its morphological, physiological and biochemical charateristics were investigated, together with antimicrobial spectrum therefrom. On antimicrobial spectrum study, substance secreted from this strain, had no activities to fungus and yeast. The selected strain showed G(+) and coccal shape, on Gram, staining and electron scanning microscopy, respectively. Biochemically this strain utilized glucose, fructose lactose, sucrose, but did not arabinose, cellulose, rhamnose, sorbitol, trehalose, mannitol. Catalase test showed negative property. Optimal growth temperature was $37^{\circ}C$. The results obtained above suggest this strain Streptococcus faecium subspp. and we named it Streptococcus sp. An-21-1.

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Kraft Lignin Biodegradation by Dysgonomonas sp. WJDL-Y1, a New Anaerobic Bacterial Strain Isolated from Sludge of a Pulp and Paper MillS

  • Duan, Jing;Liang, Jidong;Wang, Yiping;Du, Wenjing;Wang, Dongqi
    • Journal of Microbiology and Biotechnology
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    • v.26 no.10
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    • pp.1765-1773
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    • 2016
  • Wastewater containing kraft lignin (KL) discharged from pulp and paper industries could cause serious environmental contamination. Appropriate effluent treatment is required to reduce the pollution. Investigations on anaerobic bacteria capable of degrading KL are beneficial to both lignin removal and biofuel regeneration from the effluent. In this paper, an anaerobic strain capable of degrading KL was isolated from the sludge of a pulp and paper mill and identified as Dysgonomonas sp. WJDL-Y1 by 16S rRNA analysis. Optimum conditions for KL degradation by strain WJDL-Y1 were obtained at initial pH of 6.8, C:N ratio of 6 and temperature of 33℃, based on statistical analyses by response surface methodology. For a 1.2 g/l KL solution, a COD removal rate of 20.7% concomitant with biomass increase of 17.6% was achieved after 4 days of incubation under the optimum conditions. After the treatment by strain WJDL-Y1, KL was modified and degraded.

Analysis of Stress and Strain for Steel Sheet Pile Bridge Abutment (강널말뚝 교대의 응력 및 변형 해석)

  • Chung, Ha-Ik;You, Jun;Oh, In-Kyu;Eun, Seong-Woon;Son, In-Goon;Lee, Sung-Yeol;Kim, Hyung-Koo
    • Proceedings of the Korean Geotechical Society Conference
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    • 2004.03b
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    • pp.561-564
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    • 2004
  • Steel sheet pile can be alternative material for bridge abutment for. The steel sheet pile bridge abutment is new and replacement bridge abutment due to its aesthetically attractive and cost effective. Use of embedded steel sheet piling brings savings in dead load, provides a compliant retaining wall, and permits speedier construction. In addition, for replacement bridge projects, traffic interruption can be minimized. It is hoped that this study will encourage designers and constructors to consider a steel substructure option more frequently during the conceptual and preliminary design phases of projects and thereby to take advantage of the potential to construction more efficiently. In this paper, an analysis of stress and strain for steel sheet pile bridge abutment was conducted. From the analysis results, the stress and strain characteristics of steel sheet pile bridge abutment with variations of steel sheet pile parameters is suggested.

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Development of Thermal Distortion Analysis Method on Large Shell Structure Using Inherent Strain as Boundary Condition (고유변형도를 경계조건으로 갖는 대형 각(殼) 구조물 열변형 해석법 개발)

  • Ha, Yun-Sok
    • Journal of the Society of Naval Architects of Korea
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    • v.45 no.1
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    • pp.93-100
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    • 2008
  • There are two ways of conventional thermal distortion analysis. One is the thermal elasto-plastic analysis and the other is the equivalent forces method based on inherent strain. The former needs exorbitant analysis time, while the latter cannot obtain results of stress field and it needs much time consumption with loads modeling on curved plates. Such faults in two methods have made difficulties in thermal distortion analysis of a large structure like ship hull. In order to solve them, new kind of thermal distortion analysis method was developed. We devised that the inherent strains was used as direct input factors in forms of boundary conditions. It was embodied by using thermal expansion coefficient in commercial code. We used the pre-calculated inherent strain as thermal expansion coefficient, and endowed nodes with imaginary temperatures. This method was already adopted at hull block welding distortion analysis which was considered as impossible, and gave productive results such as reduction of work time in the dry dock.

Estimation of Hardness using DEFORM$^{TM}$ in SKH9 High Speed Steel (DEFORM$^{TM}$을 이용한 SKH9 고속도공구강의 경도 예측)

  • Park, Joon Hong;Sung, Jang Hyun;Kim, Young Hee;Lee, Hae Woo;Jeon, Eun Chan;Park, Young Chul
    • Journal of the Korean Society for Heat Treatment
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    • v.20 no.4
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    • pp.175-180
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    • 2007
  • The hardness of cold-forged products is in close relationship with its effective strain. This study presented the estimating method of hardness for cold-forged SKH9 products without hardness tests in view of resistance to plastic deformation using finite element code, DEFORM$^{TM}$. The flow stress equation obtained from the compression test was only used as a basic data to estimate the relationship between effective strain and hardness. In addition, this new estimating method was applied to the cold-forged product which was widely used in industrial field to show the feasibility. As a result, the predicted hardness numbers through FE simulation showed good agreement with the measured hardness numbers. It is possible to estimate the hardness not by hardness tests, but by only computer simulations for the deformed products. Also, effective strain values were possibly estimated by measuring hardness numbers, and vice versa.

Isolation and Structural Characterization of an Oligosaccharide Produced by Bacillus subtilis in a Maltose-Containing Medium

  • Shin, Kwang-Soon
    • Preventive Nutrition and Food Science
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    • v.21 no.2
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    • pp.124-131
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    • 2016
  • Among 116 bacterial strains isolated from Korean fermented foods, one strain (SS-76) was selected for producing new oligosaccharides in a basal medium containing maltose as the sole source of carbon. Upon morphological characterization using scanning electron microscopy, the cells of strain SS-76 appeared rod-shaped; subsequent 16S rRNA gene sequence analysis revealed that strain SS-76 was phylogenetically close to Bacillus subtilis. The main oligosaccharide fraction B extracted from the culture supernatant of B. subtilis SS-76 was purified by high performance liquid chromatography. Subsequent structural analysis revealed that this oligosaccharide consisted only of glucose, and methylation analysis indicated similar proportions of glucopyranosides in the 6-linkage, 4-linkage, and non-reducing terminal positions. Matrix-assisted laser-induced/ionization time-of-flight/mass spectrometry and electrospray ionization-based liquid chromatography-mass spectrometry/mass spectrometry analyses suggested that this oligosaccharide consisted of a trisaccharide unit with 1,6- and 1,4-glycosidic linkages. The anomeric signals in the $^1H$-nuclear magnetic resonance spectrum corresponded to ${\alpha}$-anomeric configurations, and the trisaccharide was finally identified as panose (${\alpha}$-D-glucopyranosyl-1,6-${\alpha}$-D-glucopyranosyl-1,4-D-glucose). These results suggest that B. subtilis SS-76 converts maltose into panose; strain SS-76 may thus find industrial application in the production of panose.