• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1201-1209
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• 2006

The purpose of this study was to isolate probiotic lactic acid bacteria (LAB) that produce bile salt hydrolase (BSH), and to evaluate its effects on serum cholesterol level. One-hundred-twenty bacterial colonies were initially isolated from human feces, and five strains were selected after screening based on their resistance to acids, tolerance against bile salts, and inhibitory activity on Escherichia coli. The Lactobacillus plantarum strain with the highest level of BSH activity was identified using 16S rRNA sequences, and was named L. plantarum CK 102. L. plantarum CK 102 at a level of 1.36$\times$10$^8$cfu/ml survived in pH 2 buffer for 6 h and exhibited excellent tolerance for bile salt. Coculturing the strain with E. coli in MRS broth resulted in strong inhibition against growth of E. coli at 18 h. Furthermore, the potential effect of CK 102 on serum cholesterol level was evaluated in rats. Thirty-two rats [Sprague-Dawley (SD) male, 129$\pm$l g, 5 weeks old] were divided into four groups of eight each. For six weeks, Group 1 was fed a normal diet (negative control); Group 2 was fed a cholesterol-enriched diet (positive control); Group 3 was fed a cholesterol-enriched diet plus L. plantarum CK 102 at 1.0$\times$10$^7$cfu/ml; and Group 4 was fed a cholesterol-enriched diet plus L. plantarum CK 102 at 5.0$\times$10$^7$cfu/ml. Blood samples were collected, serum lipids were analyzed, and weights of the organs were measured. Total blood cholesterol level, triglyceride, LDL-cholesterol, and free-cholesterol values were lower in rats that were fed 1. plantarum CK 102 than in those not fed L. plantarum CK 102. This cholesterol lowering effect implies that L. plantarum CK 102 could be utilized as an additive for health-assistance foods. In conclusion, these results suggest that the 1. plantarum CK 102 isolated could be used commercially as a probiotic.

• Journal of Yeungnam Medical Science
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• 제5권1호
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• pp.49-60
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• 1988

1984년부터 1987년까지의 최근 4년간 영남대학교 의과대학 부속병원에서 시행한 혈액배양의 분석결과 다음과 같은 결론을 얻었다. 1) 4년간 8083검체의 혈액이 배양되어 582검체에서 균이 분리되어, 총 혈액배양 양성율은 7.2%이었고, 분리균주 중 다균성 감염증은 16예(2.8%)였다. 2) 분리균주의 분포는 그람 양성균이 189, 그람 음성균이 393이었고, 그람 양성균의 균종별 분리는 Staphylococcus aureus가 82, coagulase 음성 Staphylococcus가 73, 기타 Staphylococcus sp.가 20의 순이었다. 그람 음성균은 Escherichia coli가 75, Salmonella typhi가 80, Salmonella paratyphi A가 72이고, 그 외 Kldbsiella, Enterobacter의 순으로 분리되었다. 포도당 비발효 그람 음성균은 Pseudomonas cepacia가 46, Pseudomonas aeruginosa가 16, 그 외 Acinetobacter등이 소수 분리되었다. 3) Salmonella균종을 제외한 모든 균종에서 매년 증가되는 양상을 보이고, 병원내 감염균으로 간주되는 Serratia, Acinetobacter균종이 1984년의 6.35%에서 1987년에는 17.7%롤 증가되었다. 4) Aeromonas hydrophilia는 6~10월에 높은 분리율을 나타애고, Salmonella typhi는 1월과, 5월에서 8월사이, Salmonella paratyphi A는 1월과 6월에서 9월사이에서 높은 분리율을 나타냈다. 대개 모든 균종에서 여름철에 분리율이 높았다. 5) 분리균종의 항생제 감수성 검사에서는 Staphylococcus aureus와 장내세균에서는 기본 항생제에 대한 감수성을 나타낸 반면, 포도당 비발효 균종은 약 32%에서 기본 항생제에 대한 내성을 가진 것으로 나타났으며, 병원성 감염의 주요 원인균은 Serratia, Acinetobacter, Pseudomonas, Enterobacter균종에서는 항생제의 내성이 큰 문제점으로 대두되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권9호
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• pp.1259-1265
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• 2015

The present study was performed to study the genetic relationship of productive life with production and type traits of Korean Holsteins at first three lactations. The data for the analysis from 56,054, 28,997, and 11,816 animals of first, second and third parity cows which were born from 2006 to 2011 were collected by Dairy Cattle Improvement Center, National Agricultural Co-operative Federation. Milk, protein and fat yields adjusted for 305 days and average somatic cell score considered as production traits and analyzed type traits were stature, strength, body depth, dairy form, rump angle, rump width, rear leg side view, foot angle, front attachment placement, rear attachment height, rear attachment width, udder cleft, udder depth, front teat placement and front teat length. A multi trait genetic analysis was performed using Wombat program with restricted maximum likelihood animal model composed of fixed effect of birth year, farm and the random effect of animal and random residual effect according to the traits. Heritability estimates of productive life were between 0.06 and 0.13. Genetic and phenotypic correlations between production and productive life traits ranged from 0.35 to 0.04 for milk, 0.16 to 0.05 for protein and 0.18 to 0.02 f 15-0034 (2nd) 150520 or fat. Somatic cells score showed a negative genetic and phenotypic correlation with productive life and also udder type traits, indicating that the selection for higher udder traits will likely to improve resistance to mastitis and persistence in the herd. Among all dairy form type traits, udder characters such as udder cleft showed a significant relationship with productive life. However, a specific change of heritabilities or correlations were not observed with the change of parity. Moreover, further studies are needed to further confirm the significance of the above traits and the effect of parity on above relationships in order to minimize both voluntary and involuntary culling rates while improving herd health and maintaining high yielding dairy cows.

• Fisheries and Aquatic Sciences
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• 제22권12호
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• pp.30.1-30.11
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• 2019

Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 ㎍ ml^-1 (26 μM), while fascaplysin affected the bacterial growth of V. harveyi (50 ㎍ ml^-1) and T. maritimum (0.25 ㎍). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 ㎍ ml^-1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming.

• 한국강구조학회 논문집
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• 제20권2호
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• pp.237-246
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• 2008

연속경간을 가지는 I형강 교량은 내부 지점근처에서 상대적으로 큰 부모멘트가 발생하게 되는데, 이에 경제적인 단면 활용을 위하여 내부 지점부위의 상부 및 하부플랜지에 플레이트를 보강한 변단면을 사용하고 있다. 본 연구에서는 기존 탄성 횡-비틀림 좌굴식에 관한 연구를 토대로 하여 비탄성 구간에 있는 계단식 I형보의 횡-비틀림 좌굴강도를 범용구조해석프로그램 ABAQUS(2006)를 이용하여 산정하고, 간편한 설계식을 제안하고 있다. 유한요소해석에는 4절점 쉘요소인 S4R이 사용되었고, 국내외에서 많이 사용되는 I형강 단면(${W36{\times}160}$)을 대상으로 하였다. 양단 및 한쪽 끝단에 계단식 단면을 가지는 보에 대해서 고려하였으며, 플랜지 길이방향 비, 너비방향 비, 두께의 비로 계단식 I형보를 나타내었다. 해석에 사용된 매개변수는 각각 27가지 및 36가지 조합이고, 하중조건으로 보의 순수굽힘이 발생하는 균일모멘트를 적용시켰으며, 비탄성 구간범위 내에 있는 비지지 길이에 대하여 구조해석을 수행하였다. 비탄성 횡-비틀림 거동을 보기 위하여 잔류응력 및 초기결함을 고려한 비선형해석을 실시하였는데, Pi(1995)등이 고려한 잔류응력의 형상과 국내 I형강 표준 치수 허용치에 근거하여 부재 길이의 0.1%를 초기제작오차로 고려하였다. 본 연구 결과는 다양한 형식의 I형보가 사용되는 빌딩 및 교량의 경제적이고 합리적인 설계의 근간을 제공해 줄 것이며, 향후 다양한 하중 조건을 가지는 양단 또는 일단 계단식 단면 변화보의 비탄성 횡-비틀림 좌굴강도를 계산할 수 있는 설계식 개발에 적극 활용 될 수 있을 것이다.

• 한국강구조학회 논문집
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• 제23권3호
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• pp.337-347
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• 2011

본 논문에서는 콘크리트 채움 U형 강재보와 강재 H단면 기둥으로 구성된 접합부의 내진 성능을 평가하였다. 접합부 내진성능을 평가하기 위하여 세 개의 보-기둥 접합부 실험체를 반복주기하중에 대하여 실험하였다. 합성보는 콘크리트 슬래브와 스터드를 이용하여 일체화 되었으며, 슬래브에는 부모멘트를 위한 철근이 배치되었다. 접합부 상세를 실험 변수로 하였으며, 보 접합부의 강화방안 및 약화방안, 합성효과의 정도를 고려하였다. 합성보의 춤은 슬래브 두께를 포함하여 600mm이며, 강재보와 슬래브의 철근은 H형강 기둥과 용접을 통해 접합하였다. 접합부 강화방안은 합성보 플랜지에 덧댐플레이트를 용접하였으며, 약화방안으로서 소성힌지 발생지점에 채움콘크리트 안에 스티로폼을 삽입하였다. 실험 결과 완전합성 실험체는 강도와 변형능력, 에너지 소산에 있어서 우수한 성능을 보여주었다. 변형능력은 특수모멘트골조 기준인 4% 이상의 회전각을 발휘하였다.

• 한국환경성돌연변이발암원학회지
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• 제25권2호
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• pp.51-59
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• 2005

Overexpression of HSP25 delayed cell growth, increased the level of $p21^{waf}$, reduced the levels of cyclin D1, cylcin A and cdc2, and induced radioresistance in L929 cells. We demonstrated that extracellular regulated kinase (ERK) and MAP kinase/ERK kinase (MEK) expressions as well as their activation (phospho-forms) were inhibited by hsp25 overexpression. To confirm the relationship between ERK1/2 and hsp25-mediated radioresistance, ERK1 or ERK2 cDNA was transiently transfected into the hsp25 overexpressed cells and their radioresistance was examined. HSP25-mediated radioresistance was abolished by overexpression of ERK2, but not by overexpression of ERK1. Alteration of cell cycle distribution and cell cycle related protein expressions (cyclin D, cyclin A and cdc2) by hsp25 overexpression were also recovered by ERK2 cDNA transfection. Increase in Bc1-2 protein by hsp25 gene transfection was also reduced by subsequent ERK2 cDNA-transfection. In addition, HSP25 overexpression reduced reactive oxygen species (ROS) and increased expression of manganese superoxide dismutase (MnSOD) gene. Increased activation of NF-kB (IkB degradation) was also found in hsp25-overexpressed cells. Moreover, transfection of hsp25 antisense gene abrogated all the HSP25-mediated phenomena. To further elucidate the exact relationship between MnSOD induction and NF-kB activation, dominant negative $I-kB\alpha(I-kB\alpha-DN)$ construction was transfected to HSP25 overexpressed cells. $I-kB\alpha-DN$ inhibited HSP25 mediated MnSOD gene expression. In addition, HSP25 mediated radioresistance was blocked by $I-kB\alpha-DN$ transfection. Blockage of MnSOD with antisense oligonucleotides in HSP25 overexpressed cells, prevented apoptosis and returned the ERK1/2 activation to the control level. From the above results, we suggest for the first time that reduced oxidative damage by HSP25 was due to MnSOD-mediated down regulation of ERK1/2.

• 지역사회간호학회지
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• 제9권1호
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• pp.40-63
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• 1998

A long life is the desire of many people. The purpose of this study was to describe long life factors for people aged one hundred and above on Chju island. The research was conducted using a phenomenological method to add understanding to this subject. The field work for this study was done from July, 1997 to February, 1998 on Cheju island. The sample consisted of 10 persons of one hundred or above and 12 families. They were audio-recorded and analyzed using Colaizzi's (1978) method. The results of the present study shows the following: The thoughts of the persons one hundred and above showed ten important concepts; 'dietary regimen', 'mental regimen', 'sleep regimen', 'residence regimen', 'health regimen', 'labor regimen', 'exercise regimen', 'being born', 'filial piety and posterity', 'attachment to life'. Concerning 'Dietary Regimen': Dietary regulation, Preferential food intake, Living on vegetables, Light eating, and concern for longevity in food intake were important. Concerning 'Mental Regimen' : Harmony, Clearheadedness, Mildness, Abstinence, Generosity, Relaxation, Gaiety, Inspiration and Strength were important. Concerning 'Sleep Regimen'; Enough sleeping hours, Sound sleep, Curved sleeping posture, and Comfortable bedding were important. Concerning 'Residence Regimen' ; Clean dwelling, Pleasant surroundings, Thatched house life, Living in the village with good-hearted people, and Regular life were important. Concerning 'Health Regimen'; Temperance of drug, use Proper temperature, Proper humidity, and a Clean body were important. Concerning 'Labor Regimen'; Agricultural labor, Diligence, and Domestic labor were important. Concerning Exercise Regimen': Proper exercise, and Sunbathing were important. Concerning 'Being Born' ; Strong physical constitution, Longevity lineage, and Destiny were important. Concerning 'Filial Piety and Posterity' : Showing respect to a long-lived elder and Reducing anxiety were important. Concerning 'Attachment to Life' ; The desire to live long was important. The following conclusion was made from the above results. Human beings cannot avoid death, but the span of life can be prolonged to the maximum span of human life by wisdom, health care, and proper environmental conditions. As a result, a health regimen must be imposed for longevity such as controling food intake, having positive thoughts, being relaxed, sleeping enough, clean dwelling, taking care of oneself which can improve immunity and resistance to decrease, as well as proper labor and exercise. In addition, when filial piety is served, the desire of longevity and retaining one's youthfulness for a long time can be achieved. These should be done to live long and lead a qualitative life. By the results of this research, the following is suggesed for nursing. To satisfy the basic nursing principle that nursing must be practiced to help people live long, education must be spread to people who are nursed and their family so that they can lead a healthy life. And in nursing elders, nurses must recognize that elders have a strong desire for life, even though they may have negative thoughts about life at times. Therefore nurses have to respect the elders' value of life, and help them improve their self-esteem and self -fulfilment.

• 콘크리트학회논문집
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• 제18권3호
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• pp.405-414
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• 2006

기둥단면형상, 중력하중, 슬래브 경간길이와 같은 설계변수에 따른 플랫플레이트-기둥 접합부의 거동특성을 분석하기 위하여 기존의 강도모형을 검토하고 비선형유한요소해석을 실시하였다. 기존 강도 모형은 위험단면에서의 전단응력분포를 가정함에 있어서 다양한 설계변수의 영향을 고려하지 못하여 플랫플레이트-기둥 접합부의 강도를 정확하게 예측하지 못하였다. 비선형유한요소해석 결과, 하중가력방향과 평행한 기둥폭이 길어질수록 위험단면 측면에서 비틀림 전단을 받는 유효영역과 측면최대전단강도가 줄어들어 접합부의 강도가 큰 폭으로 감소한다. 중력하중은 접합부 위험단면에 부모멘트를 재하시키므로 측면의 최대전단응력을 감소시키며 접합부의 강도와 연성도를 줄어들게 한다. 중력하중이 재하되지 않은 경우, 경간길이가 길어질수록, 위험단면의 강성이 줄어들어 접합부의 강도와 연성도가 증가한다. 반면, 중력하중이 상대적으로 크게 재하된 경우, 경간길이가 짧을수록 접합부 강도가 증가하는데, 이는 동일한 크기의 전단력이 위험단면에 재하된다 하더라도 경간의 길이가 길어질수록 접합부 주변의 부모멘트로 인한 영향을 더 크게 받기 때문이다. 이와 같은 설계변수의 영향을 고려하여 접합부 강도를 산정하기 위하여 유효최대전단응력값을 제안하였으며 이 값을 사용하여 플랫플레이트-기둥 접합부의 강도를 산정할 경우, 기존 강도평가방법보다 정확한 예측이 가능함을 수치해석과의 비교를 통하여 검증하였다.

• Tuberculosis and Respiratory Diseases
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• 제60권3호
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• pp.353-356
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• 2006

폐결핵 치료중 흉부엑스선상 악화 및 갑자기 발생한 하지 마비와 감각이상으로 자기공명영상 촬영후 급성 횡단척수염 진단 및 객담 검사상 다제내성 결핵균 검출로 2차 결핵약제와 스테로이드 병합치료를 시행하여 부분적으로 호전을 보였던 증례이다. 급성 횡단척수염은 매우 드문 질환이며 균주의 직접 침범이나 면역학적 기전으로 발생하나 후자가 더 가능성 있는 기전으로 생각되어지고 있다. 아직도 결핵 및 다제내성 결핵의 유병률이 높은 국내 상황에서 드물게 결핵이 원인으로 추정된 급성 횡단척수염의 증례를 보고하는 바이다.