• 대한한방부인과학회지
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• 제27권1호
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• pp.28-40
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• 2014

Objectives: This study was to evaluate the single dose toxicity of Sobokchuko-tang (SBC) in male and female mice. Methods: Aqueous extract of SBC (yield=6.60%) was administered to female and male mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 14 principle organs were also examined. Results: we could not find any SBC treatment related mortality and clinical signs, changes in the body and organ weights, gross findings and changes in histopathology of principle organs, except for pharmacological immunomodulatory effects related findings including significant increases of submandibular lymph node weights, hypertrophy and hyperplasia of lymphoid cells in the submandibular lymph nodes restrictly detected in 2,000 mg/kg treated female and male mice with some sporadic accidental findings. Conclusions: The results obtained in this study suggest that the 50% lethal dose and approximate lethal dose of SBC aqueous extracts in both female and male mice were considered as over 2,000 mg/kg, the limited highest dosage recommended by KFDA Guidelines, and can be safety used in clinics.

• 한국임상수의학회지
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• 제38권5호
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• pp.249-253
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• 2021

We describe the case of a Jindo dog that presented with a 2-month history of an abscess-like mass on the skin of the left hip. The patient was initially diagnosed with a suspected insect bite and was treated with a topical dressing. After observing no improvements, surgical debridement was done to remove the abscess. Nine days after surgery, the patient started showing severe anorexia, vomiting, lethargy, and oliguria. Azotemia was detected, and the patient was diagnosed with acute kidney injury. Ultrasonography depicted an abdominal mass (4.6 × 7.5 cm) in the right ureter, and severe hydronephrosis in the right kidney. The patient was euthanatized. Results of the necropsy, histopathologic examination, and immunohistochemistry for tumor cells, confirmed that the patient had developed hydronephrosis and disseminated histiocytic sarcoma which had metastasized to the lungs. To the best of our knowledge, this is the first report of acute renal failure produced by ureter invasion and urinary tract obstruction resulting from the abdominal mass of disseminated histiocytic sarcoma in dogs.

• 한국임상수의학회지
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• 제36권2호
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• pp.119-122
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• 2019

The prevailing discipline notes that primary pulmonary angiosarcoma is an extremely rare malignant tumor with almost grave prognosis when presented in a dog. No cases have been reported earlier as occurring in dogs. This is the first time we are reporting a case of primary lung angiosarcoma in a 12-year-old Yorkshire terrier breed dog, that will explore the clinical as well as histopathological features of the tumor as noted in a dog. In this case, radiography revealed a well-defined large soft tissue mass in the caudo-dorsal lung field across the left hemi-thorax. After necropsy, it is noted that the lung was found to have the blood-filled nodular lesions on its surface, as determined with no such lesions on other organs. Upon the histological examination, it showed the presence of an extensive necrotic hemorrhage with anastomosing vascular space. Later, the immunohistochemistry showed strongly positive CD31 cells confirming the endothelial origin of the tumor. This is the first report of canine primary lung angiosarcoma in the Republic of Korea.

• 한국동물위생학회지
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• 제44권2호
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• pp.119-124
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• 2021

The report describes an avian mycobacteriosis in a captive wild bird. A 7-year-old female Indian peafowl (Pavo cristatus) maintained in a zoo of Korea presented a gradual cachexia and eventually was found dead. At necropsy, severely atrophied pectoral muscles exposing the keel bone were noticed. Yellowish thick nodules in varying sizes were scattered in all lobes of lungs, liver and spleen, suggesting mycobacteriosis. Histopathologically, multifocal to coalescing granulomas surrounded by multinucleated giant cells were observed. Numbers of acid-fast bacilli were revealed in granulomas. Then, a series of molecular diagnostic techniques were followed: a nested PCR, DNA sequencing and bioinformatics analysis. It resulted as Mycobacterium genavense. The identification of M. genavense as an etiological agent suggested that it might serve as a risk factor for other captive wild animals, and for a potential zoonotic risk since M. genavense have been a definite cause of disseminated mycobacterial infection in immunocompromised people. To the authors' knowledge, this is the first report of avian mycobacteriosis with M. genavense in a captive Indian peafowl.

• 한국동물위생학회지
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• 제43권4호
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• pp.251-256
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• 2020

Two suckling piglets, 4 days and 10 days of age, showed lethargy and dyspnea after birth and mortality had been increased after incoming gilts from breeding farm. At necropsy, the lungs showed diffuse fail to collapse with rubbery consistency, edematous dilatation of interlobular septa, and lobular consolidation with purple red color. Heart was diffuse pale in color and had several irregular linear-shaped macules or patches. Histopathologically, diffuse interstitial pneumonia with the proliferation of type II pneumocytes was present in the lungs of 2 piglets. Alveolar lumens contained necrotic cellular debris derived from neutrophils and macrophages. Multifocal hemorrhage and necrotizing pneumonia with protozoan tachyzoites were observed in the lungs. Severe multifocal to confluent necrotic myocarditis, necrotic encephalitis, and necrotic adrenalitis with intralesional protozoan tachyzoites were observed in piglets. According to immunohistochemical analysis (IHC), Toxoplasma (T.) gondii tachyzoites antigens were confirmed in lung, heart, brain, and adrenal gland. And porcine reproductive and respiratory syndrome virus (PRRSV) antigens were also detected in the cytoplasm of macrophages in lungs using IHC. Based on the gross, histopathologic and immunohistochemical features, two suckling piglets were diagnosed as co-infection of T. gondii and PRRSV.

• 한국동물위생학회지
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• 제44권1호
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• pp.35-43
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• 2021

Rabbit infectious hemorrhagic fever has been reported in rabbits worldwide. The disease is also frequently reported on Korean rabbit farms, and the pathological study of 9 rabbits on such disease-occurring farms was attempted to identify the pathogen. Clinical signs were torticollis and ear ulceration. Most rabbit died with bloody nasal discharges. At necropsy, multiple hemorrhages and inflammation were observed in heart, lung, liver and uterus. The main histopathologic features were hemorrhagic suppurative meningoencephalitis, fibrinous bronchointerstitial pneumonia, bacteremia, liver cell necrosis, multifocal hemorrhages in kidney and disseminated intravascular coagulopathy. The viral VP60 gene of RHDV was identified by Reverse Transcriptase PCR. Pasteurella multocida organisms were cultured, identified by biochemical test and serotyped as A by multiplex capsular typing PCR. In conclusion, the fatal hemorrhagic disease was due to combined infection with both RHDV and P. multocida in rabbits. To our knowledge, this is the first case report about co-infection with both RHDV and P. multocida in rabbits in Korea.

• 한국동물위생학회지
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• 제44권4호
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• pp.299-303
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• 2021

A captive male polar bear (Ursus maritimus) was found dead after a long-term anorexia at a Park Zoo in Republic of Korea. At necropsy, the liver lesion was prominent with hepatomegaly carrying a big mass and numerous small nodules in various sizes. The cut surface of the nodules was creamy white, firm, and solid with umbilicated appearance. Histologically, there was proliferation of hepatocellular neoplastic cells arranged in glandular-like (pseudoglandular) pattern and complete loss of hepatocellular arrangement. The immunoreactivity for neoplastic hepatocyte antigens was positive for hepatocyte paraffin 1, and negative for vimentin, cytokeratin 7 and 19, and carcinoembryonic antigen. Based on those diagnostic features, the neoplasia was diagnosed as hepatocellular carcinoma. To the author's knowledge, this is the first case report of hepatocellular carcinoma in the captive polar bear in Republic of Korea.

• 한국임상수의학회지
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• 제38권6호
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• pp.279-284
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• 2021

A 10-year-old spayed female Yorkshire terrier dog with a history of pleural effusion and dyspnea for a week was referred to the Veterinary Teaching Hospital. Radiography revealed pulmonary edema and pleural effusion. A mass infiltrating the right atrial lumen and especially the supra-tricuspid valve was identified with echocardiography. The mass was diagnosed as intracardiac neoplasia. Symptomatic treatment was prescribed to alleviate the symptoms of heart failure. Despite symptomatic treatment, the patient died a few days later. After obtaining consent from the owner, necropsy and histopathological evaluation were performed. The result was consistent with cardiac leiomyoma, and the diagnosis was confirmed by immunohistochemical staining. To the authors' knowledge, this case is the first report of intracardiac leiomyoma in a dog in Korea.

• 한국동물위생학회지
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• 제45권2호
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• pp.125-131
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• 2022

The purpose of this study was to examine antibody titers to structural protein (SP) of the foot-and-mouth disease (FMD) virus after vaccination in animals of the Seoul zoo. After the initial inoculation of FMD vaccine to the susceptible animals of the zoo, a total of 235 blood samples were collected from 42 species of zoo animals during treatment or necropsy. All samples were tested by using enzyme-linked immunosorbent assay (ELISA). The overall positive rate of SP antibodies against FMD virus was 94.0% (221/235). However, the positive rates varied according to animal species. The results of positive rates in 30 species were 100% but in 12 species were 50-94.7%. We showed that most animals that have received FMD vaccine in Seoul zoo have been reached to the level of herd immunity against FMD virus after the vaccination. To the best of our knowledge, this study would be the first report for monitoring the vaccine-induced SP antibody titers against FMD virus after vaccination in various zoo animal species in Korea.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.253-262
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• 1998

Ochratoxin A (OA) is a potent mycotoxin causing considerable health hazard and economic loss- e,i. OA is of concern as it is hepato-nephrotoxic, mutagenic, and carcinogenic to a great variety of animals. LDso of crude OA was 8.5 mgf kg.b.w., i.p. The clinical symptoms, mortalities and necropsy were recorded in rats injected with OA (LD5o, i.p.) during 10 days of daily treatment. Ginseng treatments (20 mg 1 kg. b.w., i.p.) : before, mixed with, or after OA dose, completely prevented the mortality in rats. OA-treated animals showed microcytic normochromic anaemia, lucocytosis, hypoproteinaemia and elevation of serum ALT, AST, AP, urea, and creatinine values. These findings were declined near the normal levels when ginseng injected with OA. OA (115 LDso) induced chromosomal aberrations (65.66%) compared to the control. When ginseng given 10 min before OA injection, chromosomal aberrations were reduced to be 31.66% compared to OA-treated animals. In conclusion: ginseng has a protective effect against ochratoxicosis, it has anti-genotoxic activity and it can repair the chromosomal damage induced by ochratoxin A. Key words Ochratoxicosis, Chromosomal aberrations, Mycotoxins, Ochratoxin A, Korean gin sting, Protective effect of Panax ginseng, Rat