• 한국작물학회지
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• 제35권1호
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• pp.16-22
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• 1990

5월 보리와 조강 Near-isogenic line을 선발, 계통화하여 파성정도, 일장반응성 및 포장에서 출수관련형질을 조사하였다. 1. 5월보리는 밀장 006에서 조강보리는 Hiproly나 상주백과쪽에서 파성유전자가 각각 유래된 것으로 추측되었다. 2. 5월보리 #1과 #2, 조강보리 #1과 #2은 파성 I로, 5월보리 #3과 #4, 조강보리 #3과 #4은 파성III으로 각각 분류되었으며, 5월보리 #1과 #2은 1쌍의 파성 열성유전자(sh)에 의해 파성이 발현된 것으로 생각된다. 3. 동일한 유전적 배경을 가진 isogenic line의 일장반응성, 협의의 조만성 및 출수지연율은 계통간 통계적 유의성이 인정되지 않았다. 4. 실외 추파시 isogenic line간에 출수기와 성숙기의 차이가 인정되었으며, 3월 11일 이후 춘파시 춘파성정도의 강ㆍ약에 따라서 같은 계통내 출수개체와 좌지된 개체가 함께 관찰되었다.

• 한국작물학회지
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• 제46권1호
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• pp.12-16
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• 2001

In order to develop near isogenic lines (NILs) the heterozygous rice plants for alkali digestibility value(ADV) were selected and tested in every generation from $F_5$to $F_9$ of a cross, Seratus malam/Suwon 345. Finally several sets of NILs, which were six low ADV lines and four medium-high ADV lines, were selected among $F_{10}$ lines. No differences of the plant growth characteristics, amylose content and protein content of rice grain were found between low and medium-high ADV lines. Rice flour of low ADV lines showed longer gel length in gel consistency test than medium-high ADV lines, and also showed different gelatinization characteristics measured by rapid visco analyzer compared with medium-high ADV lines. The result of genetic analysis indicated that ADV-NILs developed were differed in a pair of major gene controlling ADV, and low ADV was dominant over medium-high ADV.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2000년도 춘계 학술대회지
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• pp.42-43
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• 2000

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.207-210
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• 2003

Four different backcrossing methods were designed and 23 near isogenic lines (NILs) of 22 linkage groups were obtained using Hb as recurrent parent, the mutant gene lines which held markers as donor parents. Eleven of them had been mated with the recurrent parent for 10 times, and the others for 7∼8 times. The NILs of other 6 linkage groups are under way and had been backcrossed to the recurrent for 3∼4 times. These NILs will act important roles in the construction of molecular linkage map and gene location and positional cloning.

• 한국작물학회지
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• 제31권2호
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• pp.226-230
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• 1986

wx 126조합으로부터 분리된 농녹색업과 담녹색엽 near-isogenic계통의 광합성 정도와 생장특성 및 수량관련형질들을 비교 검토한 결과를 요약하면 다음과 같다. 1. 농녹색엽계통이 담녹색영계통보다 엽록소 a, b 및 총엽록소 함량은 현저히 높았으나 엽록소 a, b의 비율은 큰 차이가 없었다. 2. 출수기 지엽에 있어서 단위엽면적당 광합성율은 농녹색엽계통이며 높았으나 단위엽녹소당 광합성량은 담녹색엽계통이 더 높았다. 3. 이앙부터 출수기까지의 CGR은 농녹색엽계통이 더 높았으며 이는 NAR이 높은 데서 기인된 것으로 해석되었다. 4. 농녹색엽게통의 주당수수, 주당영화수 및 주당수량은 담녹색엽계통에 비해 각각 높았으나 1,000입중과 등숙율은 두 계통간에 차이가 없었다.

• 한국작물학회지
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• 제45권4호
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• pp.221-226
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• 2000

This experiment was designed to assess changes in yield difference between waxy (IWR) and non-waxy (INWR) near isogenic rice by evaluating the translocation and accumulation of assimilates in different parts of the rice plants after heading stage under different fertilizer application. Nitrogen content of culm in both IWR and INWR decreased rapidly from 35 days before heading to flowering time and after then it was maintained constantly, while the nitrogen content of leaf blade was continually reduced. Sugar content of leaf blade in IWR decreased steadly by 10 days after heading, but that in INWR increased 20 days after heading. There was no difference in starch content of culum between IWR and INWR from 35days before heading to heading, but that of culm of IWR increased again after heading. Dry weight of IWR plant was less reduced during ripening as compared with INWR. The dry weight of culm was not significantly different between two isogenic lines.

• 한국작물학회지
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• 제51권4호
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• pp.318-321
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• 2006

This study was conducted in 2002 and 2003 to investigate variation on rice quality associated with cooking, and eating qualities under the three different fertilizer levels, none fertilizer level($N-P_2O_{5-}K_2O=0-0-0kg/ha$), ordinary fertilizer level($N-P_2O_{5-}K_2O=1l0-45-57kg/ha$), and heavy fertilizer level($N-P_2O_{5-}K_2O=180-90-110kg/ha$). The eight resistant near-isogenic lines(NILs) for bacterial blight in rice were examined for grain appearances, several physicochemical properties, and palatability value measured by Toyo taste meter. Significant variations in NILs(V), Years(Y), and VxY interaction were recognized in grain length and palatability value under the none fertilizer cultivation, in grain width under the heavy fertilizer cultivation, and in white belly ratio under the three different fertilizer cultivations, respectively. According to increase the fertilizer application rate, variation in grain length and grain width were not significant, but grain thickness was thinner under the ordinary and heavy fertilizer cultivations than under the none fertilizer cultivation. On the other hand, white belly ratio, protein content and Mg/K ratio increased, while amylose content and palatability value lowered. Alkali spreading value lowed under the heavy fertilizer cultivation than under the none and ordinary fertilizer cultivations. Palatability value was significantly low in the line carrying XalJ than in the other lines under the ordinary fertilizer cultivations.

• Journal of Crop Science and Biotechnology
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• 제11권4호
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• pp.263-268
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• 2008

Bacterial leaf pustule (BLP) caused by Xanthomonas axonopodis pv. glycines is a serious disease to make pustule and chlorotic haloes in soybean [Glycine max (L). Merr.]. While inheritance mode and map positions of the BLP resistance gene, rxp are known, no sequence information of the gene was reported. In this study, we made five near isogenic lines (NILs) from separate backcrosses (BCs) of BLP-susceptible Hwangkeumkong $\times$ BLP-resistant SS2-2 (HS) and BLP-susceptible Taekwangkong$\times$ SS2-2 (TS) through foreground and background selection based on the four-stage selection strategy. First, 15 BC individuals were selected through foreground selection using the simple sequence repeat (SSR) markers Satt486 and Satt372 flanking the rxp gene. Among them, 11 BC plants showed the BLP-resistant response. The HS and TS lines chosen in foreground selection were again screened by background selection using 118 and 90 SSR markers across all chromosomes, respectively. Eventually, five individuals showing greater than 90% recurrent parent genome content were selected in both HS and TS lines. These NILs will be a unique biological material to characterize the rxp gene.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2000년도 춘계 학술대회지
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• pp.300-301
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• 2000

• The Plant Pathology Journal
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• 제23권3호
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• pp.155-160
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• 2007

In order to develop chili pepper bacterial spot resistant cultivars and near-isogenic lines (NILs) to prompt the molecular mapping of the resistance gene, we have run backcross breeding program since 1994. Two resistance genes against Xanthomonas axonopodis pv. vesicatoria Bs2 from Fla. XVR 3-25 and Bs3 from our breeding line 25-11-3-2, were introduced into a land race, Chilseongcho (abbreviated to Chilseong hereafter) with good fruit guality. We report here the testing of $BC_4F_3\;to\;BC_4F_5$. We found that $BC_4F_5$ lines of the crosses were homozygous with respect to the respective genes of introduction. The lines, in which Bs2 gene was introduced, were hypersensitively resistant to both race 1 and race 3 of X. axonopodis pv. vesicatoria, whereas, those in which Bs3 was introduced were resistant to race 1.