• Title, Summary, Keyword: near isogenic line

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Breeding of Near Isogenic Lines of Silkworm (Bombyx mori L.)

  • Li, Muwang;Xu, Anying;Hou, Chengxiang;Zhang, Yuehua;Huang, Junting;Guo, Xijie
    • International Journal of Industrial Entomology
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    • v.6 no.2
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    • pp.207-210
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    • 2003
  • Four different backcrossing methods were designed and 23 near isogenic lines (NILs) of 22 linkage groups were obtained using Hb as recurrent parent, the mutant gene lines which held markers as donor parents. Eleven of them had been mated with the recurrent parent for 10 times, and the others for 7∼8 times. The NILs of other 6 linkage groups are under way and had been backcrossed to the recurrent for 3∼4 times. These NILs will act important roles in the construction of molecular linkage map and gene location and positional cloning.

The Effects of gl, gh and wx Gene on the Grain Yield and Yield Components of Rice Plant (수도의 gl, gh 및 wx유전자가 수도수량 및 수량구성요소에 미치는 영향)

  • 김현구
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.1
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    • pp.83-109
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    • 1977
  • Isogenic lines for glabrous leaf (gl), hull color(gh) and glutinous endosperm (wx) were used to clarify the effects of the gl, gh and wx gene on the grain yield and yield components in rice plant. Eight genotypes: glgl ghgh wxwx, glgl ghgh WxWx, glgl GH-wxwx, glgl GH-WxWx, GL-ghgh wxwx, GL-ghgh WxWx, GL-GH-wxwx and GL-GH-WxWx were evalutated in the backcrossed $F_2$ generation. Significant effects were measured for gl genotypes on heading date, gh genotypes on grain fertility, and wxwx genotypes on number of panicles per hill and 1, 000 grain weight. The grain yield of straw hull and non glutinous lines were higher than gold hull and glutinous lines. The interaction of three genes, gl, gh and wx was significant for heading date, but not significant in other characters. The average value of isogenic lines which were combined with two recessive genes and one dominant gene were lower and the C.V. value was higher than the isogenic lines which were combined with two dominant genes and one recessive gene in all characters except plant height and number of grains per plant. The average value of isogenic line combined with three recessive genes was lower and the C.V. was higher than the isogenic line combined three dominant genes in all characters measured.

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Near-Isogenic Lines for Genes Conferring Hypersensitive Resistance to Bacterial Spot in Chili Pepper

  • Kim, Byung-Soo;Kim, Young-Chun;Shin, Kwang-Sik;Kim, Jeong-Hoon
    • The Plant Pathology Journal
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    • v.23 no.3
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    • pp.155-160
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    • 2007
  • In order to develop chili pepper bacterial spot resistant cultivars and near-isogenic lines (NILs) to prompt the molecular mapping of the resistance gene, we have run backcross breeding program since 1994. Two resistance genes against Xanthomonas axonopodis pv. vesicatoria Bs2 from Fla. XVR 3-25 and Bs3 from our breeding line 25-11-3-2, were introduced into a land race, Chilseongcho (abbreviated to Chilseong hereafter) with good fruit guality. We report here the testing of $BC_4F_3\;to\;BC_4F_5$. We found that $BC_4F_5$ lines of the crosses were homozygous with respect to the respective genes of introduction. The lines, in which Bs2 gene was introduced, were hypersensitively resistant to both race 1 and race 3 of X. axonopodis pv. vesicatoria, whereas, those in which Bs3 was introduced were resistant to race 1.

Comparison of Major Agronomic Traits between Barley Near-Isogenic Line for Awnedness (보리까락길이 Near-Isogenic 계통의 주요 형질 비교)

  • 천종은
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.2
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    • pp.121-127
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    • 1993
  • The barley plant for whole crop forage is advantageous to have traits with higher photosynthetic rate, biomass, lodging resistance, and awnless spikes. The objective of this study is to investigate performance of near-isogenic line pairs for the awned and awnless in the field, and to observe the variations in photosynthetic rate, yield and their related traits under removal of flag leaf and awns. Grain yield was 6% higher, but biomass and photosynthetic rate were 6~16% and 6% lower in the awned line than those in the awnless grown in the field ,respectively. Defoliation of flag leaf resulted in approximately 3~4%, and 5~6% decreases in 1,000 kernels and grain weights, respectively. Deawning at the heading stage of the long awned line decreased 1,000 kernels and grain weights by 7% and 15%, respectively, The removal of both assimilative tissues caused 14 and 21% decrements in 1,000 kernels and grain weights. Apparent photosynthetic rate of leaf blade in awnless line was 6.1 -9. 7 $CO_2$$_2$ ${\mu}$mole $m^{-2}$ $s{-1}$, much higher than that in awned line. The relative rates in the second and third leaves were about 93% and 55~63%, respectively as compared with that in the flag leaf. The results of the experiments suggest that the awnless line selected for whole crop forage is a potential plant type with higher photosynthetic rate, leaf area and greater biomass.

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Comparison of Grain Quality of Bacterial Blight Resistant Near-isogenic Lines of Rice under Different Fertilization Levels

  • Shin Mun-Sik;Kim Ki-Young;Choi Yun-Hi;Shin Seo-Ho;Ko Jae-Kwon;Lee Jae-Kil
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.4
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    • pp.318-321
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    • 2006
  • This study was conducted in 2002 and 2003 to investigate variation on rice quality associated with cooking, and eating qualities under the three different fertilizer levels, none fertilizer level($N-P_2O_{5-}K_2O=0-0-0kg/ha$), ordinary fertilizer level($N-P_2O_{5-}K_2O=1l0-45-57kg/ha$), and heavy fertilizer level($N-P_2O_{5-}K_2O=180-90-110kg/ha$). The eight resistant near-isogenic lines(NILs) for bacterial blight in rice were examined for grain appearances, several physicochemical properties, and palatability value measured by Toyo taste meter. Significant variations in NILs(V), Years(Y), and VxY interaction were recognized in grain length and palatability value under the none fertilizer cultivation, in grain width under the heavy fertilizer cultivation, and in white belly ratio under the three different fertilizer cultivations, respectively. According to increase the fertilizer application rate, variation in grain length and grain width were not significant, but grain thickness was thinner under the ordinary and heavy fertilizer cultivations than under the none fertilizer cultivation. On the other hand, white belly ratio, protein content and Mg/K ratio increased, while amylose content and palatability value lowered. Alkali spreading value lowed under the heavy fertilizer cultivation than under the none and ordinary fertilizer cultivations. Palatability value was significantly low in the line carrying XalJ than in the other lines under the ordinary fertilizer cultivations.

Marker-Assisted Foreground and Background Selection of Near Isogenic Lines for Bacterial Leaf Pustule Resistant Gene in Soybean

  • Kim, Kil-Hyun;Kim, Moon-Young;Van, Kyu-Jung;Moon, Jung-Kyung;Kim, Dong-Hyun;Lee, Suk-Ha
    • Journal of Crop Science and Biotechnology
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    • v.11 no.4
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    • pp.263-268
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    • 2008
  • Bacterial leaf pustule (BLP) caused by Xanthomonas axonopodis pv. glycines is a serious disease to make pustule and chlorotic haloes in soybean [Glycine max (L). Merr.]. While inheritance mode and map positions of the BLP resistance gene, rxp are known, no sequence information of the gene was reported. In this study, we made five near isogenic lines (NILs) from separate backcrosses (BCs) of BLP-susceptible Hwangkeumkong $\times$ BLP-resistant SS2-2 (HS) and BLP-susceptible Taekwangkong$\times$ SS2-2 (TS) through foreground and background selection based on the four-stage selection strategy. First, 15 BC individuals were selected through foreground selection using the simple sequence repeat (SSR) markers Satt486 and Satt372 flanking the rxp gene. Among them, 11 BC plants showed the BLP-resistant response. The HS and TS lines chosen in foreground selection were again screened by background selection using 118 and 90 SSR markers across all chromosomes, respectively. Eventually, five individuals showing greater than 90% recurrent parent genome content were selected in both HS and TS lines. These NILs will be a unique biological material to characterize the rxp gene.

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Development of Near Isogenic Lines with Various Seed Sizes and Study on Seed Size-related Characteristics in Watermelon (다양한 종자크기를 가진 Near Isogenic 수박 계통 육성 및 종자관련 특성 분석)

  • Kim, Yong-Jae;Yang, Tae-Jin;Park, Young-Hoon;Lee, Yong-Jik;Kang, Sun-Cheol;Kim, Yong-Kwon;Cho, Jeoung-Lai
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.403-411
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    • 2009
  • We inspected seed sizes of 353 genetic accessions of watermelon to diversify functional utility related to seed size and classified them into six representative groups based on their seed sizes. Each group was named as giant seed (GS), big seed (BS), medium size (NS), small size (SS), micro seed (MS) and tomato seed (TS) from the biggest. As the seed size was getting smaller, decreased seed length and seed width, increased seed number per fruit, and decreased seed weight per fruit were observed, but seed shape did not change significantly. In order to study the effect of seed size on fruit weight and seed germination, we developed three near isogenic lines (NILs) with three different seed sizes, SS, MS and TS, from crossing between two accessions 'NT' and 'TDR', and one NIL with seed size of TS from crossing between two accessions 'S55' and 'TDR'. In the study on the fruit weight of NILs with various seed sizes, NS, SS, MS, and TS NILs produced an average of 6.4, 6.3, 5.9, and 4.2 kg fruits, respectively. The bigger seed types showed the better germination rate. NS type showed the highest germination percentage, while TS showed very low germination percentage. Fermentation treatment for 48 hrs increased the germination percentage on TS type seed, but still remained at a low level. In NS, SS, and MS material, the ratio of embryo/whole seed weight was over 50%, meanwhile that of TS was only 44.4% of which low embryo percentage would be one of the reason of low germination percentage. From this study, we concluded that watermelon has very wide genetic diversity on seed size which is somehow related to fruit sizes and germination rate.

Interspecific Competition of Paddy Rice Isogenic Lines in Plant Type with Some Perennial Weeds (수도초형(水稻草型)의 Isogenic Line과 다년생(多年生) 잡초(雜草)의 경합특성(競合特性) 연구(硏究))

  • Kim, I.K.;Guh, J.O.;Kwon, S.L.
    • Korean Journal of Weed Science
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    • v.3 no.1
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    • pp.39-49
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    • 1983
  • By use of three paddy rice lines as the near-isogenic in plant type (Broom, Open, and Spread type in tillering angle), the interspecific competition patterns of rice plants with three important weed species (Cyperus serotinus, Eleocharis kuroguwai, and Potamogeton distinctus) under the three densities of weed standing (0.25 and 50 percent of the rice plants), were observed. Under the experimented conditions, paddy yields were varied more significantly with weed competition descriptions than with plant types of paddy rice. And spread typed rice was more competitive to the detected weed species, however, the broom and spread typed rice were to Potamogeton SP, among others. The result of the clustering analysis of crop-weed competition patterns, estimated by 1-Q mode correlation coefficients, indicated that the first-order component affecting the competition patterns of crop-weed was rather the plant types of rice than either weed species or weed standing densities.

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Development of the pyramiding lines with strong culm genes derived from crosses among the SCM near isogenic lines in rice

  • Ookawa, Taiichiro;Kamahora, Eri;Ebitani, Takeshi;Yamaguchi, Takuya;Murata, Kazumasa;Iyama, Yukihide;Ozaki, Hidenobu;Adachi, Shunsuke;Hirasawa, Tadashi;Kanekatsu, Motoki
    • Proceedings of the Korean Society of Crop Science Conference
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    • pp.21-21
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    • 2017
  • Severe lodging has recurrently occurred at strong typhoon's hitting in recent climate change. The identification of quantitative trait loci (QTLs) and their responsible genes associated with a strong culm and their pyramiding are important for developing high-yielding varieties with a superior lodging resistance. To identify QTLs for lodging resistance, the tropical japonica line, Chugoku 117 and the improved indica variety, Habataki were selected as the donor parent, as these had thick and strong culms compared with the temperate japonica varieties in Japan such as Koshihikari. By using chromosome segment substitution lines (CSSLs) in which chromosome segments from the japonica variety were replaced to them from Habataki, we identified the QTLs for strong culm on chrs. 1 and 6, which were designated as STRONG CULM1 (SCM1) and STRONG CULM2 (SCM2), respectively. By using recombinant inbred lines (BILs) derived from a cross between Chugoku 117 and Koshihikari and introgression lines, we also identified the other QTLs for strong culm on chrs. 3 and 2, which were designated as STRONG CULM3 (SCM3) and STRONG CULM4 (SCM4), respectively. Candidate region of SCM1 includes Gn1 related to grain number. SCM2 was identical to APO1, a gene related to the control of panicle branch number, and SCM3 was identical to FC1, a strigolactone signaling associated gene, by performing fine mapping and positional cloning of these genes. To evaluate the effects of SCM1~SCM4 on lodging resistance, the Koshihiakri near isogenic line (NIL) with the introgressed SCM1 or SCM2 locus of Habataki (NIL-SCM1, NIL-SCM2) and the another Koshihikari NIL with the introgeressed SCM3 or SCM4 locus of Chugoku 117 (NIL-SCM3, NIL-SCM4) were developed. Then, we developed the pyramiding lines with double or triple combinations derived from step-by-step crosses among NIL-SCM1 NIL-SCM4. Triple pyramiding lines (NIL-SCM1+2+3, ~ NIL-SCM1+3+4) showed the largest culm diameter and the highest culm strength among the combinations and increased spikelet number due to the pleiotropic effects of these genes. Pyramiding of strong culm genes resulted in much increased culm thickness, culm strength and spikelet number due to their additive effect. SCM1 mainly contributed to enhance their pyramiding effect. These results in this study suggest the importance of identifying the combinations of superior alleles of strong culm genes among natural variation and pyramiding these genes for improving high-yielding varieties with a superior lodging resistance.

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