• Ocean and Polar Research
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• 제36권2호
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• pp.199-208
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• 2014

Copepods are a major food source for marine fish larvae in nature. Many studies on copepods culture have been conducted to develop a new live food for the seedling production of marine fish larvae. But fish farmers still depend on rotifer and Artemia nauplii. This study was carried out to find suitable copepods as live food for the larvae in hatchery. Eight species of copepods (1 calanoid, 2 cyclpoid, 5 harpacticoid) that were fed Isochrysis galbana were examined in terms of the size of nauplii, fecundity, amino acids, and fatty acids contents. These species were divided into small (nauplii length 46-86 ${\mu}m$) and large (nauplii length 120-188 ${\mu}m$) size group. Nitokra spinipes in the small group and Tigriopus japonicus in the large group showed the highest fecundity with 151.1 and 139.6 nauplii production per gravid female, respectively. With regard to nutrients, essential amino acids were the highest with 21.2% in cyclopoid Paracyclopina nana in the small group and n-3 HUFA were the highest in calanoid Pseudodiaptomus inopinus (8.5 ${\mu}g/mg$) in the large group and P. nana (8.8 ${\mu}g/mg$). In terms of the size, fecundity, and nutritional value of copepods examined in this study, N. spinipes and P. nana seem to be suitable copepod species to develop as a new live food for small mouth fish larvae.

• 한국양식학회지
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• 제19권2호
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• pp.109-118
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• 2006

체색의 변화가 다양하여 관상용으로 가치가 높은 Hippocampus coronatus의 안정적인 번식기술을 개발하기 위하여 가막만과 여자만 부근해역의 잘피밭에서 채집 한 후 실험실에서 사육하면서 어미의 출산특성, 자치어의 성장 및 초기자어사육에 알맞은 먹이조건을 조사하었다. $7{\sim}11$월에 가막만과 여자만에서 출현한 체장(SL) $53.91{\sim}87.31 mm$ (평균 $77.76{\pm}9.84 mm$, n=12) 범위의 개체들이 실험실내에서 $6{\sim}75$ 개체(평균 39 ind./male/time)를 출산하였다. 출산 직후 자어의 크기는 $11.69{\sim}15.81 mm$(평균 $13.7{\pm}1.3 mm$, n=15)였고 몸통과 꼬리의 체륜수는 10 그리고 39개였으며 가슴지느러미, 등지느러미 그리고 뒷지느러미 줄기수는 각각 12, 14 그리고 4개였다. 출산 후 60일에는 $23.48{\sim}25.29 mm$ (평균, $24.65{\pm}0.83 mm$, n=4)로 성장하였고 일간성장률은 0.18 mm/day였다. Copepod와 Artemia를 혼합 공급하여 포식량을 조사한 결과 출산 후 5일째에 copepod nauplii의 포식량은 평균 21 ind./larva/hour였고 Artemia napulii의 포식량은 평균 3 ind./larva/hour로 copepod nauplii에 대한 선호도가 높았고 출산 후 30일째에는 copepod nauplii에 대한 포식량이 평균 5 ind./larva/hour, 그리고 Artemia napulii의 포식량이 평균 9 ind./larva/hour로 성장함에 따라 Artemia napulii에 대한 포식량이 증가하였다. 생존율을 고려한 먹이 공급방법에 있어서 Artemia nauplii와 copepod nauplii를 혼합 공급할 경우 생존율은 출산 후 50일에 48.9%로 향상된 결과를 얻을 수 있었다.

• 한국수산과학회지
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• 제49권5호
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• pp.642-647
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• 2016

Postlarval and juvenile anchovies Engraulis japonicus were collected using a gape net with wings in the coastal waters of Yeosu in July and August 2015, and their gut contents were analyzed. The size range of the postlarvae was 11.0-24.2 mm (notochord length) and that of the juveniles was 25.1-37.4 mm (standard length). Guts were empty in 64.5% of postlarvae and in 51.7% of juveniles. The dominant prey organisms in the guts of both postlarvae and juveniles were copepoda. Calanoida were important prey organisms for postlarvae and were dominated by nauplii of Paracalanus sp. and Calanus sp. as well as unidentified copepod naupill. Calanoida were also important in the diets of juveniles, whose gut contents were dominated by Paracalanus sp. and Cirripedia. Gut compositions were significantly different (P<0.05) among three body size levels in postlarvae and juveniles. As the body size of the postlarvae and juveniles increased, Calanoida constituted a larger proportion of the gut contents, while the proportion of copepod nauplii decreased.

• 환경생물
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• 제29권4호
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• pp.379-387
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• 2011

Copepod nauplii plays an important role as a linker between the microbial food web and classical food chain in marine ecosystem and is an essential food source for early stage of many larval fishes. Study on the influencing factors on the growth of copepod naupliar stages has been rarely carried out in despite of these ecological significances. Many studies have shown that food availability and temperature are major factors to influence copepod growth. However, due to the complicated environment parameters in coastal ecosystem, the relationships between growth of copepods and influencing factors are still unclear under the natural condition. Growth rates of the copepod $Acartia$ $hongi$ nauplii were measured in Kyeonggi Bay from February to December 2001. $Acartia$ $hongi$ is numerically abundant and widespread predominant species in the coastal regions of the Yellow Sea and occurs continuously throughout the year, with a maximum peak in late spring. The naupliar growth rates of $Acartia$ $hongi$ by the artificial cohort method varied from 0.03 to 0.18 $day^{-1}$, with a mean of 0.09 $day^{-1}$. The overall naupliar growth rates showed a significantly positive relationship with the variation in water temperature. However, Previous study reported that the growth rates of adult $Acartia$ $hongi$ were primarily influenced by the variation in chlorophyll-$a$. Therefore, these differences demonstrated that the influencing factors of growth did not correspond with the developmental stages. The results of this study suggest that the dissimilarity of growth between nauplius and adult female resulted from the size-dependant difference in food availability and the growth of older developmental stages containing adults are more food-dependent than juveniles.

• Animal Systematics, Evolution and Diversity
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• 제10권2호
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• pp.217-229
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• 1994

The napuliar stages of Harpaciticus nipponicus Ito reared in the laboratory are descirbed and illustrated in detail. Ovigerous females of h. nipponicus were collected from the intertidal zone of Kuryongpo Yougil-gun Kyongangbuk-do. Korea. This species passed through 6 naupilar stages before metamorphosis to the first copepodid stage. At $20^{\circ}C$, the first copepo양 stage was attained in 6-8 (mean 7 ) days after hatching. The nauplii of Harpacticus species can be distinguished from those of other Harpacticid genera by the maxillule, pereiopods, and caudal setae. The nauplii of h. nipponicus are similiar in morphology to those of H. uniremis but distinguished by the differences in naupliar size and appendage setation. A key to identify the naupliar stages of this speices is briefly discussed.

• 한국수산과학회지
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• 제35권4호
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• pp.445-450
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• 2002

A series of 29 sampling with a 330 ${\mu}$m and a 64 ${\mu}$m mesh size of nets was conducted at a fixed station in Ilkwang Bay, southeast cost of Korea, from Oct, 2, 1991 to Oct. 10, 1992, to investigate the effects of mesh size of nets on biomass estimation of copepod Acartia steueri. The catch of copepodite and nauplius stages of A. steueli taken by two nets with different mesh size was different, showing that all developmental stages of A. steueri were retained on the 64 ${\mu}$m mesh net, but only $\geq$stage 4 copepodite were caught by the 330 ${\mu}$m mesh net. Abundance and biomass in each developmental stage estimated with the 64 ${\mu}$m mesh net were significantly higher than those of the 330 ${\mu}$m mesh net, except for adult female and stage 5 copepodite in female. The body length as well as the body width is likely to affect the catch of the nets. The mean biomass of A. steueli estimated with the traditional 330 ${\mu}$m net was 2.8 times lower than the value obtained with the 64 ${\mu}$m mesh net. However, the seasonal patterns of the biomass were comparable. These results suggest that accurate sampling strategr of the entire copepods assemblage including nauplii and copepodites are essential when estimating the abundance and biomass of copepods for the better understanding of the role of copepods in marine ecosystem.

• 한국양식학회지
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• 제12권4호
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• pp.309-315
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• 1999

In order to understand the initial feeding time of larvae red marbled rockfish, Sebastiscus tertius changes of mouth size were investigated. Also, the fecundity of different size of red marbled rockfish broodstocks was measured. The broodstocks of red marbled rockfish, S. terius of $32.3\pm6.52 cm$ and larger than 45cm in total length spawned 296,648 and 600,000 larvae, respectively. About 50% was normal healthy larvae among the spawned ones. Mouth sizes calculated by the angle mouth opening and upper jaw length for newly spawned red marbled rockfish larvae were 0.263, 0.197 and 0.132 mm at D, 0.75D and 0.5D, respectively, when the upper jaw length was 0.186mm. Thereafter, 20 days old larvae showed 0.822, 0.617 and 0.411 mm in calculated mouth length at D, 0.75D and rockfish larvae grows, their upper jaw length gradually increased. 2 days old red marbled rockfish larvae possibly stated to feed rotifers and lasted their feeding for the another 35days, relatively long periods compared with other larvae fish. And 25 days old larvae possibly fed the newly hatched Artemia nauplii and lasted their feeding for the another 20 days, like other larvae fish. The proper supplying time of artificial feeds for the red marbled rockfish larvae seemed to be around 10 days after spawning.

• 한국해양학회지:바다
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• 제8권2호
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• pp.78-93
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• 2003

경기만 표영 생태계에서 미소형 및 소형 동물플랑크톤의 군집구조와 생태학적 역할을 조사하기 위하여, 1997년 12월부터 1998년 11월까지 한달 간격으로 미소형 및 소형 동물플랑크톤의 생물량 분포와 군집구조를 조사하였다. 본 조사에서 미소형 및 소형 동물플랑크톤은 2~200 $\mu\textrm{m}$ 크기의 종속영양 미세생물로 정의 하였으며, 종속영양 미소 편모류, 빈 섬모충류와 유종 섬모충류를 포함하는 섬모충류, 종속영양 와편모류와 동물플랑크톤 유생으로 구분하였다. 종속영양 미소 편모류의 현존량과 탄소량은 각각 330~4,370 cells m $l^{-1}$(평균: 1,340$\pm$130 cells m $l^{-1}$), 0.63~12.4 $\mu\textrm{g}$C 1$^{-1}$(평균: 4.35$\pm$0.58 $\mu\textrm{g}$C 1$^{-1}$)로 분포하였고, 부유 섬모충류의 현존량과 탄소량은 각각 338~44,571 cells 1$^{-1}$(평균: 3,526$\pm$544 cells 1$^{-1}$), 1.3~119.7 $\mu\textrm{g}$C 1$^{-1}$(평균: 13.7$\pm$3.0 $\mu\textrm{g}$C 1$^{-1}$)로 분포하였고, 종속영양 와편모류의 현존량과 탄소량은 각각 88~47,461 cells 1$^{-1}$(평균: 9,034$\pm$2,347 cells 1$^{-1}$), 0.04~54.05 $\mu\textrm{g}$C 1$^{-1}$(평균: 6.9$\pm$ 2.4 $\mu\textrm{g}$C 1$^{-1}$)로 분포하였고, 동물플랑크톤 유생의 현존량과 탄소량은 각각 5~546 indiv. 1$^{-1}$(평균: 83$\pm$15 indiv. 1$^{-1}$), 0.17~43.2$\mu\textrm{g}$C 1$^{-1}$(평균: 6.3$\pm$1.2$\mu\textrm{g}$C 1$^{-1}$)로 분포하였다. 조사기간 동안 미소형 및 소형 동물플랑크톤 각 군집의 생물량은 유종 섬모충류를 제외하고는 조석에 의한 차이를 보이지 않았다. 표층부터 저층까지 합산한 미소형 및 소형 동물플랑크톤의 탄소량은 124~l,635 mgC $m^{-}$$^2$/(평균: 585$\pm$110 mgC $m^{-2}$ )로 분포하였으며, 3월과 5월에 가장 높게 나타났다. 미소형 및 소형 동물플랑크톤 중에서 부유 섬모충류가 가장 우점하는 그룹으로 나타났으며, 전체 미소형 및 소형 동물플랑크톤의 평균 42.3%를 기여 하였다. 미소형 및 소형 동물플랑크톤에 대한 각각의 그룹들의 상대적인 기여도는 미소형 및 소형 동물플랑크톤의 군집구조와 계절에 따라 다르게 분포하는 것으로 나타났다. 경기만 표영 생태계서 미소형 및 소형 동물플랑크톤의 군집구조는 식물플랑크톤의 크기별 분포 양상과 유사한 경향을 보였으며, 이 결과는 미소형 및 소형 동물플랑크톤과 식물플랑크톤 간에 피식-포식자의 관계가 있음을 암시하며, 피식-포식의 관계가 미세생물 먹이망에서 중요한 조절 요인임을 보여준다.

• Parasites, Hosts and Diseases
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• 제43권4호
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• pp.141-147
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• 2005

A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a microscope slide glass on which a cover glass of $22{\times}40mm$ in size was mounted at a height of 0.8mm. The culture medium was constituted by bottled mineral water boiled with grains of Glycine max (soybean). Chilomonas paramecium, a free-living protozoan organism, was provided as live food. Growth of nauplii hatched from eggs to the first stage of copepodite took an average of 7.7 days, and the growth of copepodite 1 to the egg-bearing adult female took an average of 20.1 days in the microculture cell with an average life time of 44.7 days. Continuous passage of cope pods was successfully maintained as long as sufficient medium and food were provided. The microculture method enables an in situ microscopic observation on the growth and developmental process of helminth larvae experimentally infected to copepods as well as of copepod itself. Furthermore, it does not require anesthetization and, therefore, minimize the amount of stress exposed to cope pods during the handling process.

• 한국수산과학회지
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• 제48권4호
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• pp.498-506
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• 2015

We examined the feeding habits of larval Clupea pallasii using 148 specimens collected from December 2010 to April 2011 in the Nakdong River estuary, Korea. We found that larval C. pallasii [8.3-26.0 mm NL (notochord length)] are carnivorous, consuming mainly copepods, rotifers, cirripeds, and free-living flatworms (Macrostomida). Their diet also include small quantities of shrimp nauplii, tintinnids, cladocerans, and larval bivalves, among other food sources. To evaluate ontogenetic changes in dietary composition, we split larvae into four size groups: 8-12 mm, 12-16 mm, 16-20 mm, and > 20 mm NL. Rotifers were the most important prey items for the smallest group, but all other groups predominantly consumed copepods. The largest group frequently preyed on rotifers, cirripeds and flatworms, in addition to copepods. Larvae also showed bi-modal feeding, with peaks in the late afternoon and at midnight, wherein the mean number of prey per individual peaked at 1,800-1,900 h.