• Korean Journal of Environmental Agriculture
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• v.22 no.1
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• pp.26-35
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• 2003

An investigation was carried out to evaluate the effects of pebble size on the small-scale sewage treatment apparatus by natural purification method that consisted of aerobic and anaerobic plot. pH and EC in both plots varied minimally regardless of pebble size. DO in aerobic plot increased as the pebble size decreased. That in the anaerobic plot was slightly less in comparison with that of the aerobic plot but varied minimally, $2.4{\sim}5.1\;mg/L$ regardless of pebble size. Under all experimental conditions, removals of BOD, COD and turbidity in anaerobic plot were more than 98, 91 and 98, 98% respectively. Removals of T-N and T-P increased as pebble size decreased. Under all experimental conditions, removals of T-N and T-P in anaerobic plot were about $45{\sim}59$ and $480{\sim}96%$, respectively. Judging from the above results, it should be considered that the optimum pebble size and pebble permeability in both plot was $2{\sim}4$ and $0.1{\sim}4\;mm$, respectively.

• Korean Journal of Environmental Agriculture
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• v.25 no.1
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• pp.14-24
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• 2006

To develop small-scale sewage treatment apparatus for detached house of agricultural village, a small-scale sewage treatment apparatus by natural purification method that consisted of aerobic and anaerobic bed was constructed. To reduce the area of a sewage treatment apparatus, four different fitter media were used and each filter medium was coarse sand, broken stone, steel slag, and mixed fitter media (coarse sand : broken stone : steel slag = 1:1:1). The efficiency of sewage treatment according to the depth of aerobic and anaerobic bed and the volume ratio of aerobic to anaerobic bed were investigated in small-scale sewage treatment apparatus. The removal rate of pollutants according to the depth of aerobic and anaerobic bed in small-scale sewage treatment apparatus was high in the order of 50 cm < 70 cm < 90 cm. The removal rate of pollutants according to the ratio of aerobic to anaerobic bed in small-scale sewage treatment apparatus was high in the order of 1:1 < 1:2 $\fallingdotseq$ 1:3. Under the optimum conditions, removal rate of BOD, COD, SS, T-N and T-P were $98{\sim}99,\;95{\sim}97,\;99,\;65{\sim}66\;and\;96{\sim}99%$ respectively, in small-scale sewage treatment apparatus.

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.284-289
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• 2004

A PCR assay for the rapid detection of Vibrio vulnificus strains was developed using a virulence gene for elastase found in various Vibrio species. The DNA sequences in the elastase gene facilitated the identification of a species-specific probe for pathogenic V. vulnificus strains from both clinical and environmental sources. Using an elastase gene-based PCR reaction, a species-specific 507-bp PCR product was visualized by agarose gel electrophoresis. Three different DNA extraction methods were then compared to improve the simplicity and rapidity of detection. A PCR assay using the conventional DNA extraction or boiling method was able to detect as few as 25 V. vulnificus cells, making the detection limits at least 1-log-scale lower than that for the EDT A-treated DNA extraction method. In particular, the boiling method, which does not require purification of the chromosomal DNA, was very effective in terms of simple and rapid detection. Meanwhile, the detection limit in a mixed bacterial culture that included other bacteria, such as Escherichia coli or Bacillus subtilis, was two V. vulnificus cells, which was 1-log-scale lower than that for the control. Accordingly, when coupled with a new DNA extraction method, the elastase gene-based PCR can provide a rapid, specific, and sensitive method for identifying V. vulnificus in clinical and environmental samples.

• Computers and Concrete
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• v.26 no.1
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• pp.31-52
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• 2020

This paper investigates the size dependent effect on the vibration analysis of a porous nanocomposite viscoelastic plate reinforced by functionally graded-single walled carbon nanotubes (FG-SWCNTs) by considering nonlocal strain gradient theory. Therefore, using energy method and Hamilton's principle, the equations of motion are derived. In this article, the effects of nonlocal parameter, aspect ratio, strain gradient parameter, volume fraction of carbon nanotubes (CNTs), damping coefficient, porosity coefficient, and temperature change on the natural frequency are perused. The innovation of this paper is to compare the effectiveness of each mentioned parameters individually on the free vibrations of this plate and to represent the appropriate value for each parameter to achieve an ideal nanocomposite plate that minimizes vibration. The results are verified with those referenced in the paper. The results illustrate that the effect of damping coefficient on the increase of natural frequency is significantly higher than the other parameters effect, and the effects of the strain gradient parameter and nonlocal parameter on the natural frequency increase are less than damping coefficient effect, respectively. Furthermore, the results indicate that the natural frequency decreases with a rise in the nonlocal parameter, aspect ratio and temperature change. Also, the natural frequency increases with a rise in the strain gradient parameter and CNTs volume fraction. This study can be used for optimizing the industrial and medical designs, such as automotive industry, aerospace engineering and water purification system, by considering ideal properties for the nanocomposite plate.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1307-1314
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• 2015

Leukemia inhibitory factor (LIF) is a member of the IL-6 cytokine family, having pleiotropic actions such as maintaining stem cell pluripotency and enabling blastocyst implantation. Because the action of LIF is mediated by a ligand-receptor interaction with the LIF receptor (LIF-R), an antagonist for LIF-R has been developed to inhibit LIF-induced signaling. In this study, we present a novel method for the production and purification of an antagonist to human LIF-R (hLA). His-tagged hLA was expressed in E. coli, and simple purification methods without any endopeptidase cleavage were designed. In addition, we determined the optimal temperature conditions for enhancing the production of soluble hLA. Finally, the bioactivity of His-tagged hLA was examined using STAT3 phosphorylation and receptivity of human endometrial ECC-1 cells. Our strategy provides a rapid and efficient method to produce biologically active recombinant hLA.

• Journal of the Semiconductor & Display Technology
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• v.20 no.4
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• pp.89-95
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• 2021

As the influence of fine dust on society spreads gradually, the public's interest in indoor air is increasingly rising. Air-purifying plants are drawing keen attention due to their natural purifying function enabled by plant physiology. However, as their fine dust reduction mechanism is limited to adsorption only, vegetation bio-filters that optimize purification effects through integration with air-conditioning systems is rising as an alternative. In accordance with the relevant standard test methods, this study looked into the fine dust reduction assessment method by air-conditioning airflow volume that can be used for the industrial spread of vegetation bio-filters. In the case of PM₁₀ at 300 ㎍/m³, it was in the order of EG-B(3,500CMH, 29 min.) < EG-A (2,500CMH, 37 min.) < CG(0CMH, 64 min.) for reaching the maintenance level (100 ㎍/m³) of publicly used facilities. For reaching the WHO Guideline(50 ㎍/m³) requirement, it was in the order of EG-B (51 min.) < EG-A (160 min.) < CG (170 min.). In the case of PM_2.5, it was in the order of EG-B (26 min.) < EG-A (33 min.) < CG (57 min.) for reaching the maintenance level (50 ㎍/m³) of publicly used facilities. It was in the order of EG-B (48 min) < EG-A (140 min) < CG (158 min) for reaching the WHO Guideline (25 ㎍/m³) requirement. The findings from the analysis showed that fine dust can be reduced most efficiently when the system is operated at 3,500CMH level. The limitation of this study is that due to the absence of a way of assessing the stress of plants in vegetation bio-filters, generating optimal air-conditioning air flow of the relevant system and economics analysis against the existing facility-type air purification system have been clarified, which should be explored further though follow-up studies.

• Korean Journal of Environmental Agriculture
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• v.25 no.1
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• pp.25-33
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• 2006

Objectives of this experiment were to select of the optimum water plant and to investigate the removal efficiency of the major nutrients in the constructed wetland by natural purification method for municipal sewage treatment in the farming and fishing village. For the experiment we used "constructed wetland" which was consisted of aerobic (vertical filter system) and anaerobic systems (horizontal filter system). Both systems were filled with gravel and filter media, and grew water plants on top of them. And then, we investigated several items such as sewage treatment efficiency, growth status of water plants and the absorbed amount of inorganic element in water plants with periodical periods. In aerobic area, removal efficiencies of BOD, COD, T-N and T-P were over 92%, 74%, 25% and 57%, respectively, and then when the water is passed through anaerobic area, the efficiency was over 96%, 84%, 44% and 71%, respectively, which was increased more treatment efficiency than that of aerobic area. Absorption amount of nitrogen and phosphorous in Miscanthus sacchariflorus BENTH were the highest in the water plants as 17.7 and 2.41 g/plant in the aerobic area, respectively. Absorption amounts of nitrogen and phosphorous in Scirpus tabernaemontani GMEL were the highest in the water plants as 8.7 and 1.13 g/plant in the anaerobic area, respectively. For the selection of optimum water plants in the constructed wetland by natural purification method for municipal sewage treatment, it were observed that there were Miscanthus sacchariflorus BENTH, Phragmites japonica STEUD and Phragmites communis TRINIUS in the aerobic area and were Zizania latifolia TURCZ, Scirpus tabernaemontani GMEL, Typha orientalis PRESL, Iris pseudoacorus L and Cares dispalata BOOTT in the anaerobic area.

• Korean Journal of Environmental Agriculture
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• v.25 no.2
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• pp.129-137
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• 2006

A study was conducted to investigate the behavior of pollutant forms at each area in the sewage treatment plant by natural purification method. The sewage treatment plant by natural purification method that consisted of aerobic and anaerobic area was constructed. The efficiency of sewage treatment depending on the operation time, the loading amount of pollutant season variation were investigated. The removal amount of BOD, COD, TOC and SS in the aerobic area was significantly increased as the treatment proceeded. Decreased BOD, COD, TOC, SS, T-N and T-P depending on the loading amount of pollutants in the aerobic and ananerobic area were mostly insoluble BOD (IBOD), insoluble COD (ICOD), soluble TOC (STOC), volatile SS (VSS), dissolved T-N (DTN) and dissolved T-N (DTP) types, respectively. The removal efficiency of BOD, COD, TOC, SS, T-N and T-P in hot season (summer and autumn) were more than that in cold season (spring and winter). The removal efficiencies of BOD, COD, TOC, SS, T-N and T-P of the effluent were about 92, 89, 73, 95, 46 and 84% in all seasons, respectively.

• Archives of Pharmacal Research
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• v.22 no.4
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• pp.367-371
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• 1999

Effects of several durgs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released form cisplatin, lipopolysaccardie (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.

• Bulletin of the Korean Chemical Society
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• v.35 no.6
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• pp.1625-1632
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• 2014

A concise and efficient one-pot synthesis of chroman-2,4-dione-based HKA derivatives by three component reaction of HKAs, triethoxymethane and 4-hydroxycoumarin derivatives under solvent-free and catalyst-free conditions is described. This protocol has many advantages, in that the GAP (Group-Assistant-Purification) chemistry process is involved in this method. As a result, the experimenter can avoid cumbersome process steps such as traditional chromatography and recrystallization purifications. The desired products can be easily obtained by washing the crude products with 95% EtOH.